OBJECTIVE:To establish the quality standard of Zhizha jianwei granules.METHODS:TLC was used for the identification of Fructus Aurantii Immaturus and Fructus Crataegi.HPLC was applied for the determination of Hesperidin by which Diamonsil C18(200 mm?4.6 mm,5 ?m)chromatographic column was used and the mobile phase was acetonitrile-0.2%phosphoric acid(20∶80)with a flow rate of 1.0 mL?min-1.The column temperature was maintained at 40 ℃ and the detection wavelength was set at 283 nm.RESULTS:TLC method was proved to be specific and reproducible,and free from the interference of the negative control substance.The linear range of Hesperidin was 0.102～1.122 ?g(r=0.999 8)and its average recovery rate was 99.23%(RSD=0.20%,n=6).CONCLUSION:The established standard is suitable for the quality control of Zhizha jianwei granules.

Objective To analyze the effect of health education and caring experience on the patients of non neonatal acquired deficiency of vitamin K dependent coagulation factor deficiency (VKDCF) due to rodenticide poisoning.Methods A total of 55 patients diagnosed with acquired deficiency of VKDCF through determining anticoagulant rodenticide concentration in blood were provided with health education and rational medication for 6 months.The patients with unclear poisoning reasons and definite diagnosis were analyzed and given corresponding nursing measures.Results All patients received treatment and corresponding nursing measures.Hemorrhage improved quickly in 2 days and was under control in 1 week.No recurrence happened in half year follow-up visit.Conclusions Systematic treatment and standardized health education are clinically effective on the patients of non neonatal acquired deficiency of VKDCF,and can gain active cooperation and favorable comment of the patients.

ObjectiveTo evaluate the role of the spinal cord proteasome in chronic morphine tolerance in rats.MethodsTwenty-four healthy male SD rats in which intrathecal catheters were successfully placed without complications were randomized into 4 groups ( n =6 each):normal saline group ( group NS),chronic morphine tolerance group (group M),chronic morphine tolerance + proteasome inhibitor MG-132 group (group M + MG) and MG-132 group (group MG).Normal saline 10 μl,morphine 10 μg,morphine 10 μg+ MG-132 2.5 μg and MG-132 2.5 μg were injected intrathecally twice a day for 7 consecutive days in groups NS,M,M + MG and MG respectively.Tail flick latency was measured on day 1 before intrathecal injection and on day 1,3,5 and 7 of intrathecal injection to calculate the percentage of maximum possible antinociceptive effect (MPAE).After the last intrathecal injection,5 rats were sacrificed,and L3-5 spinal cord was removed for determination of the expression of glutamate-aspartate transporter (GLAST) and excitatory amino acids carrier 1 (EAAC1)by Western blot.Results MPAE was gradually decreased during the intrathecal injection in groups M and M + MG( P ＜ 0.05).Compared with group NS,MPAE was gradually incresed during the intrathecal injection in groups M and M + MG,and the expression of GLAST and EAAC1 in the spinal cord was significantly down-regulated in group M (P ＜ 0.05).There was no significant difference in the parameters mentioned above between group MG and group NS ( P ＞0.05 ).Compared with group M,MPAE was significantly increased and the expression of GLAST and EAAC1 in the spinal cord was significantlyup-regulatedingroupM+MG(P＜0.05or0.01).ConclusionSpinal cord proteasome is involved in the development of chronic morphine tolerance in rats.

Objective To study the plasma lactic acid and blood gas and their value in the umbilical artery of the newborns. Methods The umbilical artery blood samples of 62 newborns were obtained immediately after delivery and then blood gas analysis and plasma lactic acid values were measured within ten minutes. Results The mean value of umbilical artery plasma lactic acid was:(4.4?1.7)mmol/L,pH:7.22?0.07,Base excess:(-5.4?2.7)mEq/L. The lactic acid value had a significant negative relationship with pH values (r=-0.86,P

Neutrophils play important role in anti - tumor response of tumor-bearing host as a kind of important effector cells. In order to identify the anti-tumor mechanisms of G-CSF gene therapy, we investigated the number and functions of the peripheral neutrophils in the C-26 colon adenocarcinoma-bearing mice receiving G - CSF gene therapy and high - dose chemotherapy. After G - CSF gene therapy, the number of neutrophils in peripheral blood was increased markedly in C-26 mice receiving high - dose 5 - Fu as compared with control groups including in vivo administration of rhG - CSF. The more potent cytotoxicity to C - 26 cells could be detected. The phagocytic activity, the secretion of IL-1, TNF, NO of the neutrophils were significantly enhanced. These data showed that G - CSF gene therapy can increase the number of neutrophils, activate the functions more effectively than in vivo administration of rhG - CSF.

Granulocyte colony - stimulating factor (G - CSF) is a hematopoietic growth factor that is responsible for the differentiation and proliferation of hematopoietic progenitor cells to mature granulocyte, and can increase the number of peripheral neutrophils. It has been demonstrated that it could inhibit the metastasis of the murine tumors in spontaneous and experimental metastasis models by in vivo administration of recombinant human G - CSF. In order to examine the antitumor effect of G - CSF gene therapy on mice receiving high - dose chemotherapy, C - 26 colon adenocarcinoma - bearing mice which were prepared by S. c. injection of 1?105C-26 cells were i. p. injected with rhG-CSF (2?g/day?14day) or implanted with 1?107 collagen encapsulated NIH3T3-G-CSF cells which secrete high level of G - CSF after gene transfection. In our experiment, rhG-CSF could inhibit the tumor growth and extend the survival time of early stage C-26 bearing mice. However, G - CSF gene therapy could inhibit the tumor growth and prolong the survival both in early or middle stage C-26 mice. The results showed that both rhG - CSF and G - CSF gene therapy have exact antitumor effect and G - CSF gene therapy show more effective than rhG - CSF in vivo. Then we investigated the therapeutic effects of G - CSF gene therapy on C-26-bearing mice receiving high - dose chemotherapy (5-Fu 150mg/mice i. p.) . More effective results could be observed in C-26 - bearing mice receiving high dose chemotherapy after G - CSF gene therapy. The results also suggested that G-CSF gene therapy can inhibit the tumor growth more effectively both in C-26-bearing mice or C-26-bearing mice receiving high - dose chemotherapy.

Objective To investigate the effect of cytochrome P_ 450 2A6 (CYP2A6) genetic polymorphisms on serum concentration of sodium valproate. Methods A total of 98 epileptic patients receiving sodium valproate after a period of more than 5 half-time were recruited. The genotypes of CYP2A6 of the patients were detected by nested-primer polymerase chain reaction (PCR) to examine the alleles CYP2A6*1 and CYP2A6*4. Fluorescence polarization immunoassay (FPIA) was used to measure the serum concentration of sodium valproate. Results Of the 98 cases, 73 (74.5%) were wild genotypes, 24 (24.5%) were CYP2A6*1/*4 genotypes and 1(1.0%) was CYP2A6*4/*4 genotype. According to the genotypes of CYP2A6 the patients were divided into two groups,one was group A (CYP2A6*1/*1) and the other was group B (CYP2A6*1/*4 or *4/*4). The mean value of the serum concentration of sodium valproate of the patients in group A(4.1393?0.2793) was higher than that in group B(3.3486?0.3919) with a statistical significance (P

OBJECTIVE To know the present status of endoscopes′ cleaning and disinfection in hospitals in Wuxi,(to find) the problem of existence in endoscopes disinfection management,and puts forward the suggestion.(METHODS) According to Technical Operation Standard for Endoscopes Cleaning and Disinfection(2004)′s(requirement),a survey and sampling of disinfected endoscopes were carried out. RESULTS Every hospital all had the rules and regulations of endoscopes management,personnel training rate was 50.00%,personnel protection fitting rate was 64.71%,the rate of the room special for disinfection was 35.29%,cleaning and disinfection(installation) reaching the designated position rate was 41.18%,the standard rate of cleaning and disinfection order was(11.76%,) the fitting rate of endoscopes to preserve inside condition was 23.53%,the eligible rate of(endoscopes) disinfectants in the use was 100.00%,the disinfecting eligible rate of body surface such as attraction bottle,cleaning slot,enzyme slot and washing slot was 100.00%,and the eligible rate of disinfected endoscopes in use and in preserve were 50.00% and 36.36%,respectively.CONCLUSIONS Our hospital is developing endoscope standard including the use of disinfectants as well as the object surface disinfectiong,but all exist the problems in personnel training,personnel protection,speciaized disinfection room,basic cleaning and disinfection(installation),the process of cleaning and disinfection,preservation and disinfection effect of endoscopes,and the work(remains) further standardize.

Objective To explore the X ray imaging of the common complications after total hip replacement (THR), in order to improve the diagnosis and differential diagnosis of the complications of THR. Methods Forty six cases were revised because of the complications of THR. The value of X ray in the diagnosis of complications was assessed by observing the radiography and comparing with surgical results. Results In these cases, after revision, 34 cases had aseptic loosening of femoral component, 13 cases had aseptic loosening of acetabular component, presenting on radiographs as the lucent zone alone the component, which was ≥2 mm in width and had the same contour as the component. 4 cases had septic loosening of femoral and (or) acetabular component, presenting on radiographs as the lucent zone alone the component, and bone destruction and sinus were also observed. The lucent zone was also ≥3 mm, but had the different contour of the component. Both kinds of loosening may accompany with migration of the component or not. 6 cases had dislocation of femoral head, presenting on radiographs as the head lying out of acetabulum in 2 cases, central migrating of the head in 2 cases, and asymmetrical position of the head in acetabulum in 2 cases. Conclusion The lucent zone and component migration had the important diagnostic value of loosening, the contour of lucent zone and sinus could help to make the differential diagnosis of aseptic and septic loosening. Asymmetrical position of the head in acetabulum and the head lying out of acetabulum were the signs of dislocation.

ObjectiveTo discuss the clinical value of transcutaneous bilirubin assay and serum bilirubin assay in diagnosis and treatment of neonatal hyperbilirubinemia.MethodsSelected 180 cases of hyperbilirubinemia in term newborn infants using standardized JH20-1C transcutaneous bilirubin testing non-invasive transcutaneous bilirubin determination,and compared with serum bilirubin measurement.ResultThere was significant positive correlation between transcutaneous bilirubin[51.3-325.6 (169.3 ± 51.2) μ mol/L ] and serum bilirubin [ 68.4-338.9 ( 187.5 ± 42.5 ) μ mol/L ] (r =0.91,P ＜ 0.01 ).Conclusion Transcutaneous bilirubin measurement is a non-invasive,easy operation,reliable results,there is higher value of clinical application for the detection and diagnosis of neonatal hyperbilirubinemia.