Zebrafish as a new animal model is gradually being recognized. Easy fed, growing rapidly,transparent embryo, strong reproduction, especially similar with human in gene structure, body regulation patterns, and the development of organs and disease, all those characteristics make zebrafish significant in development of vertebrate animal and human disease. This review focuses on discussing the applications of the major organs of zebrafish in the studies of developmental pediatrics.

OBJECTIVE:To observe therapeutic efficacy and safety of flupentixol and melitracen combined with pregabalin in the treatment of painful diabetic peripheral neuropathy(PDPN). METHODS:150 cases diagnosed as PDPN were selected and ran-domly divided into control group A,control group B and combination group,with 50 cases in each group. All patients received ba-sic therapy such as the control of blood glucose,blood pressure and blood lipid. Control group A was additionally given mecobala-mine 1 mg added into 0.9% Sodium chloride solution,qd,ivgtt,2 weeks later,given Mecobalamine tablet 0.5 mg,po,tid;con-trol group B was additionally given pregabalin 75 mg,po,bid;combination group was additionally given Flupentixol and melitra-cen tablet,10 mg,bid,on the basis of control group B. 3 groups were given 4 weeks treatment. After treatment,VAS score, HAMD score,and SNCV and MNVC of median nerve and peroneal nerve were compared among 3 groups. Clinical efficacy and ADR were observed. RESULTS:After treatment,VAS score and HAMD score of combination group were significantly lower than control group A and B,with statistical significance(P<0.05). SNCV and MNCV of median nerve and peroneal nerve in combina-tion group were significantly higher than in control group A and B,with statistical significance(P<0.05).The effective rate of com-bination group(90.0%)was significantly higher than that of control group A(56.0%)and B(74.0%),with statistical signifi-cance(P<0.05). No serious ADR was found in 3 groups during treatment. CONCLUSIONS:Flupentixol and melitracen combined with pregabalin have definite therapeutic efficacy in the treatment of PDPN with good safety.

Neuronal apoptosis is one of the important forms of neuronal death in ischemic stroke.N-methyl-D-aspartate (NMDA) receptor-mediated excitatory amino acid toxicity plays an important role in neuronal apoptosis. It is the initiator and executor of apoptosis in neuronal cells. NR2B is the major regulatory subunit of this receptor, its transmembrane segment M2 is a reflexed membrane loop region facing cytoplast toward intramembrane, forming the inner wall of ion channel, which determines the permeability of the NMDA receptor ion channel to calcium. Glutamate mediates cell injury mainly through calcium overload, therefore, NR2B subunit plays a vital role in the neuronal apoptosis in ischemic stroke.

Objective To identify the differentially expressed genes and functional enrichment pathways using bioin?formatics technology in mice who were acutely administered with Morphine. Methods First, we downloaded microarray da?tasets of mice which were acutely administered with Morphine from Gene Expression Omnibus, and assess the quality control parameters of the microarray datasets using AffyQCReport 1.42.0 package by R programming language 3.1.0 software. Subse?quently, the differentially expressed genes of the microarray datasets were identified using the linear models for microarray data of R language. Finally, functional pathways of the differentially expressed genes were enriched based on expression anal?ysis and systematic explorer. Results The microarray datasets showed preferable uniformity and intension similarity. A to?tal of 481 differentially expressed genes including Gm11627, Zfand4, Zbtb16, Pkp2 and Plin4 were identified. While 8 func?tional enrichment pathways, including pathways in cancer, melanogenesis and mitogen-activated protein kinase signaling were revealed. Conclusion The differentially expressed genes and functional pathways were the underlying biomarkers of mice who were acutely administered with Morphine.

Methamphetamine (MA) is a highly addictive psychostimulant that has effects on central nervous system.Chronic MA useis linked to serious neurotoxic effects and ultimately cognitive impairments, thus understanding the effect of chronic MA use on cognitive function can improve the treatment of MA abusers and reduce MA relapse. In the present study, we summarized the main results of animal experiments, neuropsychological studies and brain imaging studies on MA abuse and cognitive impairment, which may provide the basis for future research and clinical treatment.

Objective To study the clinical significance of reticulocyte parameters change before and after chemotheraphy in acute leukemia.Methods Thirty-two chemotherapeutic patients suffered from acute leukemia were improved after chemotherapy.The percentage of reticulocyte(RET%),reticulocyte absolute value(RET#),low fluorescent reticulocyte percentage(LFR),middle fluorescent reticulocyte percentage(MRF),high fluorescent reticulocyte percentage(HFR),white blood cells(WBC) and absolute neutrophils count (ANC) and immature reticulocyte fraction(IRF) were detected by using the Sysmex 2000 automatic blood cell analyzer.Results IRF,MFR and HFR after chemotherapy in the patients with leukemia were always declined,reached the lowest on 7 d of chemotherapy,began to recover on 14 d after chemotherapy,and recovered to the level before chemotherapy on 21 d of chemotherapy.LFR began to increase on 7 d of chemotherapy,and began to decrease on 14 d of chemotherapy.WBC and RET# were always decreased on 7,14 d of chemotherapy,and began to recover on 21 d of chemotherapy.Conclusion IRF,MFR,HFR and LFR are the sensitive indicators reflecting the bone marrow recovery and are earlier than WBC and RET# by 7 d.

To cultivate advanced nursing talents is the fundamental demand of nursing education in twenty-first century.This paper elaborated on the present application of university cooperative learning in clinical nursing practice aiming at combining the teaching theory with clinical practice,arousing nursing students' interest in learning and improving their team spirits and communication skills.Meanwhile,it is also conducive to the development of innovative experiment and further improvement of practical teaching quality.

ObjectiveTo explore the effects of brucine on multiple myeloma (MM) and to compare the effects between brucine and bontezomib on MM.MethodsMTT method was used to determine the median inhibitory concentratiom (IC50) of brucine and bortezomib on the MM cell line U266.The supernatant of cultured U266 cell line was added to the culture system for inducing the differentiation of osteoblast cell line MC3T3-E1.After aseptic assay,RT-PCR was used to determine the RNA levels of alkaline phosphatase (ALP),osteocalcine (OC),osteoprotegerin (OPG) and recepter activator of NF-κB ligand (RANKL).Results IC50 of bortezomib on U266 cell line for 48 h was 22.4 nmol/L,and that of strychnine was 0.16 nmol/L.The mRNA levels of ALP,OC and OPG in osteoblast co-intervened by brucine combined with the supernatant of MM cells were higher than those in supernatant of U266 cells,while the level of RANKL mRNA was lower (P ＜0.05).The degree of increasing or reducing was greater than the level of control group intervened only by bortezomib (P ＜0.05).ConclusionThe therapeutic effects of brucine on MM might be carried out through the regulation of osteoclast by osteoblast,and the experiment confirmed that the therapeutic effect of brucine on MM was superior to that of bortezomib.

Objective To study the expression of CXCL12 and E-cadherin in condyloma acuminatum (CA) lesions caused by low-risk type (HPV6 and HPV11) humanpapilloma viruses. Methods The expressions of CXCL12 and E-cadherin mRNA in the lesions of CA caused by HPV6 and HPV11 and in the foreskins from normal males as controls were detected by real-time fluorescent quantitative PCR. Results The expressions of CXCL12 in the lesions of CA were down-regulated (fold change = 20.23,P = 0.001), and the expressions of E-cadherin were up-regulated (fold change = 3.129,P > 0.05). Conclusions The expression of CXCL12 is lower and the expression of E-cadherin is higher in the lesions of CA caused by low-risk HPV. It suggests that HPV may suppress local immune response and change the adhesion between langerhans cells and keratinocytes , which may contribute greatly to the persistent infection of HPV and relapse of CA.

BACKGROUND:Timely and scientific food supplement or fortified food could promote intellectual development.Xinjiang is located in the westem part of China where inhabited by minority nationalities.Ethnic minorities have their own unique lifestyle and dietary habits.Whether their unique ways will affect infant and child nutrition requires further study.OBJECTIVE:Through investigating the nutrition and intellectual development of 454 infants and children from Uygur,Han,Kazak nationality,to recognize the factors affecting intellectual development of children.DESIGN:Sample investigation.SETTING:Department of Nutrition and Food Hygiene,College of Public Health,Xinjiang Medical University.PARTICIPANTS:Totally 454 infants and children from Tulufan,Urumqi and Hutubi where inhabited by Uygur,Han,Kazak nationality were enrolled in the investigation from May 2004 to April 2005 after obtaining their family members'agreement.METHODS:The method was that one card for one people in the 454 infants and children,and the questionnaires included linking index,the social factors related to intellectual development,prandium history,the kinds and timing of supplementary food added,the timing to add supplementary food and so on.Denver Developmental Screening Test was used to measure the intellectual ability.MAIN OUTCOME MEASURES:The kinds and timing of supplementary food added,feeding patterns,mean score of intelligence in infants and children.RESULTS:Among the 454 included infants and children,12 were dropped out,because of unqualified data,so 442 were involved in the result analysis.①Adding supplementary food:Kazaks took milk product as dominant food,and meat.vegetables and fruits were added after one year old. Ugyurs took a little meat as main food,while the Hans took eggs and grains as dominant food.②Comparison of timing to add supplementary food:Mean timing to add supplementary food were 6.87, 5.40 and 8.17 months in Uygur,Han, Kazak nationality, respectively,and there were significant differences (F=14.62,P<0.01).③Intellectual development:The score of the three national infantile intellectual ability test was that:Hans>Uygurs>Kazaks,only when over 24 months year old Kazaks>Uygurs.There were significant differences (P<0.01).④Effect of different feeding ways and timing to add supplementary food on the development of intellectual ability:The earlier the timing to add supplementary food,the higher the score was (P<0.05).There was no significant difference in intelligent score of infants and children of different feeding ways (P>0.05).CONCLUSION:It is good for the development of intellectual ability that parents improve the lactating mother,infantile nutritional condition and add supplementary food(weaning food)in early age.