Zebrafish as a new animal model is gradually being recognized. Easy fed, growing rapidly,transparent embryo, strong reproduction, especially similar with human in gene structure, body regulation patterns, and the development of organs and disease, all those characteristics make zebrafish significant in development of vertebrate animal and human disease. This review focuses on discussing the applications of the major organs of zebrafish in the studies of developmental pediatrics.

OBJECTIVE:To observe therapeutic efficacy and safety of flupentixol and melitracen combined with pregabalin in the treatment of painful diabetic peripheral neuropathy(PDPN). METHODS:150 cases diagnosed as PDPN were selected and ran-domly divided into control group A,control group B and combination group,with 50 cases in each group. All patients received ba-sic therapy such as the control of blood glucose,blood pressure and blood lipid. Control group A was additionally given mecobala-mine 1 mg added into 0.9% Sodium chloride solution,qd,ivgtt,2 weeks later,given Mecobalamine tablet 0.5 mg,po,tid;con-trol group B was additionally given pregabalin 75 mg,po,bid;combination group was additionally given Flupentixol and melitra-cen tablet,10 mg,bid,on the basis of control group B. 3 groups were given 4 weeks treatment. After treatment,VAS score, HAMD score,and SNCV and MNVC of median nerve and peroneal nerve were compared among 3 groups. Clinical efficacy and ADR were observed. RESULTS:After treatment,VAS score and HAMD score of combination group were significantly lower than control group A and B,with statistical significance(P<0.05). SNCV and MNCV of median nerve and peroneal nerve in combina-tion group were significantly higher than in control group A and B,with statistical significance(P<0.05).The effective rate of com-bination group(90.0%)was significantly higher than that of control group A(56.0%)and B(74.0%),with statistical signifi-cance(P<0.05). No serious ADR was found in 3 groups during treatment. CONCLUSIONS:Flupentixol and melitracen combined with pregabalin have definite therapeutic efficacy in the treatment of PDPN with good safety.

Objective To identify the differentially expressed genes and functional enrichment pathways using bioin?formatics technology in mice who were acutely administered with Morphine. Methods First, we downloaded microarray da?tasets of mice which were acutely administered with Morphine from Gene Expression Omnibus, and assess the quality control parameters of the microarray datasets using AffyQCReport 1.42.0 package by R programming language 3.1.0 software. Subse?quently, the differentially expressed genes of the microarray datasets were identified using the linear models for microarray data of R language. Finally, functional pathways of the differentially expressed genes were enriched based on expression anal?ysis and systematic explorer. Results The microarray datasets showed preferable uniformity and intension similarity. A to?tal of 481 differentially expressed genes including Gm11627, Zfand4, Zbtb16, Pkp2 and Plin4 were identified. While 8 func?tional enrichment pathways, including pathways in cancer, melanogenesis and mitogen-activated protein kinase signaling were revealed. Conclusion The differentially expressed genes and functional pathways were the underlying biomarkers of mice who were acutely administered with Morphine.

Methamphetamine (MA) is a highly addictive psychostimulant that has effects on central nervous system.Chronic MA useis linked to serious neurotoxic effects and ultimately cognitive impairments, thus understanding the effect of chronic MA use on cognitive function can improve the treatment of MA abusers and reduce MA relapse. In the present study, we summarized the main results of animal experiments, neuropsychological studies and brain imaging studies on MA abuse and cognitive impairment, which may provide the basis for future research and clinical treatment.

To cultivate advanced nursing talents is the fundamental demand of nursing education in twenty-first century.This paper elaborated on the present application of university cooperative learning in clinical nursing practice aiming at combining the teaching theory with clinical practice,arousing nursing students' interest in learning and improving their team spirits and communication skills.Meanwhile,it is also conducive to the development of innovative experiment and further improvement of practical teaching quality.

Objective To discuss the factors that affect the examination of urine formed elements by IQ200 automated uri-nalysis analyzer causing false positive and look for the solution methods. Methods 2,319 urine samples were tested by IQ200 automated urinalysis analyzer and microscopy. The results were contrasted and the false positive reasons were ana-lyzed. Results When using IQ200 automated urinalysis analyzer,the false positive rate of red cell is 14.7%. Crystals,bac-teria,yeast like fungus sometimes are regarded as red cell by analyzer. The false positive rate of white cell is 15.75%. Small round cells,lots of bacterium can cause false positive; the false positive rate of cast is 38.58 % and viscose rayon is main agent. Conclusion Many factors can affect the examination in IQ200 automated urinalysis analyzer and cause false positive,so IQ200 can only be used for scanning,which is very important to observe sediments by microscopic examination when pathologic ingredients in urine are increased and at the same time all above interferences are positive and manifold.

AIM: To establish the method of determining five anthraquinone components in Shaotangshang Gel(Rhizoma Coptidis,Cortex Phellodendri Chinensis,Radix et Rhizoma Rhei,etc.). METHODS:HPLC was used to determine emodin,chrysophanol,rhein,physcion,alone-emodin in Shaotangshang Gel.The separation was performed on Yilited-C_(18) Column with MeOH-phosphate acid(0.1%)(80∶20) as the modile phase,the flow rate was 1.0 mL/min and detection wavelength was set at 430 nm. RESULTS: The resolution and the linearity of the method were good with the average recoveries and RSD values of emoldin,chrysophanol,rhein,physcion,aloe-(emodin) of(98.0%),1.3%;97.1%,1.8%;97.8%,1.5%;97.4%,1.7%;96.7%,1.2% respectively.CONCLUSION: This method is sensitive,accurate and can be used for the quality control of Shaotangshang Gel.

Objective To study the expression of CXCL12 and E-cadherin in condyloma acuminatum (CA) lesions caused by low-risk type (HPV6 and HPV11) humanpapilloma viruses. Methods The expressions of CXCL12 and E-cadherin mRNA in the lesions of CA caused by HPV6 and HPV11 and in the foreskins from normal males as controls were detected by real-time fluorescent quantitative PCR. Results The expressions of CXCL12 in the lesions of CA were down-regulated (fold change = 20.23,P = 0.001), and the expressions of E-cadherin were up-regulated (fold change = 3.129,P > 0.05). Conclusions The expression of CXCL12 is lower and the expression of E-cadherin is higher in the lesions of CA caused by low-risk HPV. It suggests that HPV may suppress local immune response and change the adhesion between langerhans cells and keratinocytes , which may contribute greatly to the persistent infection of HPV and relapse of CA.

BACKGROUND:Timely and scientific food supplement or fortified food could promote intellectual development.Xinjiang is located in the westem part of China where inhabited by minority nationalities.Ethnic minorities have their own unique lifestyle and dietary habits.Whether their unique ways will affect infant and child nutrition requires further study.OBJECTIVE:Through investigating the nutrition and intellectual development of 454 infants and children from Uygur,Han,Kazak nationality,to recognize the factors affecting intellectual development of children.DESIGN:Sample investigation.SETTING:Department of Nutrition and Food Hygiene,College of Public Health,Xinjiang Medical University.PARTICIPANTS:Totally 454 infants and children from Tulufan,Urumqi and Hutubi where inhabited by Uygur,Han,Kazak nationality were enrolled in the investigation from May 2004 to April 2005 after obtaining their family members'agreement.METHODS:The method was that one card for one people in the 454 infants and children,and the questionnaires included linking index,the social factors related to intellectual development,prandium history,the kinds and timing of supplementary food added,the timing to add supplementary food and so on.Denver Developmental Screening Test was used to measure the intellectual ability.MAIN OUTCOME MEASURES:The kinds and timing of supplementary food added,feeding patterns,mean score of intelligence in infants and children.RESULTS:Among the 454 included infants and children,12 were dropped out,because of unqualified data,so 442 were involved in the result analysis.①Adding supplementary food:Kazaks took milk product as dominant food,and meat.vegetables and fruits were added after one year old. Ugyurs took a little meat as main food,while the Hans took eggs and grains as dominant food.②Comparison of timing to add supplementary food:Mean timing to add supplementary food were 6.87, 5.40 and 8.17 months in Uygur,Han, Kazak nationality, respectively,and there were significant differences (F=14.62,P<0.01).③Intellectual development:The score of the three national infantile intellectual ability test was that:Hans>Uygurs>Kazaks,only when over 24 months year old Kazaks>Uygurs.There were significant differences (P<0.01).④Effect of different feeding ways and timing to add supplementary food on the development of intellectual ability:The earlier the timing to add supplementary food,the higher the score was (P<0.05).There was no significant difference in intelligent score of infants and children of different feeding ways (P>0.05).CONCLUSION:It is good for the development of intellectual ability that parents improve the lactating mother,infantile nutritional condition and add supplementary food(weaning food)in early age.