1.The Descriptive Analysis of Exercise Participation of Residents in China
Guangsheng MA ; Dechun LUAN ; Yanpin LI
Chinese Journal of Prevention and Control of Chronic Diseases 2006;0(01):-
Objective To describe the exercise participation of residents in China,and to provide the scientific basis for developing intervention strategies.Method The data of 2002 China National Nutrition and Health Survey was used for the analysis.66 682 subjects(male 32 287,female 34 395) aged 6 years and above were involved.Results The overall rate of exercise participation of people in China was 15.7%,while that of urban residents(27.5%) was higher than that of the rural counterparts(11.1%).of all age-groups,18~years group had the lowest rate of exercise participation.8.0% people engaged in regular exercise while 7.7% people in occasional exercise.More urban residents(16.8%) engaged in regular exercise than their rural counterparts did(4.5%).The rates of regular exercise of residents in 6~years and 13~years groups were lower than those of occasional exercise,but the rates of regular exercise of people in 18~yrs,45~yrs and over 60~yrs groups were higher than those of occasional exercise.The types of exercise varied obviously among different age groups.Conclusion The rate of exercise participation of people in China is quite low.The guideline for physical activity of different target population in China should be developed in order to improve their participating rate of exercise and improve their health.
2.Screening of Protein Interacting with HCMV UL130 Protein by Yeast Two Hybrid from Human Fetus Brain cDNA Library
Gaowei REN ; Xin CUI ; Ying QI ; Yanpin MA ; Qiang RUAN ; Zhengrong SUN
Journal of China Medical University 2010;(6):413-416
Objective To screen the human proteins interacting with human cytomegalovirus(HCMV)UL130 from human fetus brain cDNA library by GAL4 two-hybrid system 3 technique and analyze the corresponding coding sequences.Methods The "bait plasmid"(named as pGBKT7-UL130)was constructed.By using HCMV UL130 as the bait,a human fetus brain cDNA library was screened and the proteins interacting with UL130 protein were searched.The positive clones were sequenced and analyzed by bioinformatic methods.Results Nine clones interacting with HCMV UL130 were identified,two of them were synaptosome-associated protein(SNAP).Conclusion Some proteins interacting with HCMV UL130 in human fetus brain cDNA library were successfully screened.SNAP might play an important role in HCMV infection pathogenesis.
3.Effect of interferon-α and thymopentin on the mRNA expression of APOBEC3A and APOBEC3B in HepG2.2.15 cells
Fang XIONG ; Yao GAO ; Yanpin MA
Journal of Clinical Hepatology 2020;36(1):76-79
ObjectiveTo investigate the effect of synergistic intervention of interferonα (IFNα) and thymopentin (TP5) on the mRNA expression of apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A (APOBEC3A) and apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3B (APOBEC3B) in HepG2.2.15 cells. MethodsHepG2.2.15 cells were divided into blank control group, IFNα treatment group, TP5 treatment group, and IFNα+TP5 treatment group, and at 12, 24, 48, and 72 hours of treatment, quantitative real-time PCR was used to measure the mRNA expression of APOBEC3A and APOBEC3B in HepG2.2.15 cells. An analysis of variance was used for comparison of continuous data between multiple groups, and the least significant difference t-test was used for further comparison between two groups. ResultsCompared with the blank control group, the IFNα treatment group and the IFNα+TP5 treatment group had a significant increase in the mRNA expression of APOBEC3A at 12, 24, 48, and 72 hours of treatment (all P<0001). Compared with the IFNα treatment group, the IFNα+TP5 treatment group had a significant increase in the mRNA expression of APOBEC3A at these four time points (all P<0.001). TP5 treatment had no significant influence on the mRNA expression of APOBEC3A at each time point (all P>0.05). There was no significant difference in the mRNA expression of APOBEC3B between the blank control group and the treatment groups (all P>0.05). ConclusionIFNα combined with TP5 can significantly upregulate the mRNA expression of APOBEC3A in HepG2.2.15 cells.