Objective To summarize the perioperative nursing of undergoing radical cystectomy with ileal neobladder for the treatment of bladder cancer? Method The nursing measures included preoperative psychological care,bowel and body preparation as well as postoperative nursing of drainage tube,ileal bladder irrigation,bladder functional exercise and basic nursing care? Results No patients suffered from postoperative complications such as infections,urinary fistula and anastomotic leakage? Twenty patients recovered autonomous urination within 3-6 months after the operation? One patient got minor urinal incontinence? Conclusions Cystectomy with orthotopic ileal neobladder trauma contributes to more complications due to large surgical trauma? Therefore,good perioperative care is important for the successful operations?

Objective To analyze the clinical characteristics of Candida parapsilosis septicemia of prematurity and provide a reference for both diagnosis and treatment.Methods The clinical data of 9 premature suffering with candida parapsilosis septicemia were retrospectively analyzed.Results All 9 cases (0.73 percent in the corresponding period) were premature infants with gestational age less than 37 weeks.The average weight of the patients was (1 621.00 ±372.22) g,among which 5 cases were of very low birth weight and 4 cases were of low birth weight.The average time of the fungi infection was (22.00 ± 7.58) d.Of the 9 cases,7 cases had been treated with broad spectrum antibiotics previously,9 cases were given nutrition intravenously,7 cases were treated with mechanical ventilation,7 cases adopted peripherally inserted central catheter(PICC) and 1 case received surgery.The symptoms included poor response,unstable serum oxygen saturation,jaundice,poor perfusion,abdominal distention,fever and no bodyweight increase.Laboratory tests results presented platelet decrease(6 cases),C-reaction protein increase (6 cases),high plasma (1-3)-β-D glucan level(9 cases) and white blood cells in the normal range(6 cases).All 9 infants recovered after receiving fluconazole and supporting treatment.Two cases failed to complete the hospital treatment but after discharge from hospital continued treatment by administering prescribed medicine,and through regular follow-up was done,the prognosis of all cases turned out to be good,and no case died.Conclusions Gestational age,birth weight,application of broad spectrum antibiotics,mechanical ventilation and peripherally inserted central catheter are common high risk factors of Candida parapsilosis septicemia in premature infants.The disease was featured with normal white blood cell,low platelet,high serum levels of C-reaction protein and (1-3)-β-D glucan.Anti-fungi therapy with fluconazole may be a good choice for it.

Objective:To study the influence of Sinapine on the formation of microvessels in chick embryo chorioallantoic membrane(CAM).Methods: Sinapine was purified by the procedure of pressurized-solvent extraction from Semen Sinapis Albae.The inhibition effect of Sinapine on the chorioallantoic vessel was detected by the chick embryo chorioallantoic membrane model.Results: Compared with physiological saline group,large areas of blood vessels in Sinapine groups(5.0g/L and 10.0g/L) had heavy inhibition of angiogenesis on CAM,accompanied by the decreased density of vessels(P0.05).Conclusions: Sinapine can prevent the formation of microvessels of CAM and the effects depend on the concentration.

Objective To explore the impacts of magnetic fields of different intensities on the superoxide dismutase (SOD) activity and malondialdchvde (MDA) levels in rat pancreatic islet cells under normal and hypoxic conditions.Methods Rat pancreatic islet cells were culured,and after 3 days were subjected to a magnetic field of either 44.8 mT,90.6 mT or 182.1 mT under either normal or hypoxic conditions.Control cells received no magnetic field exposure,SOD activity and MDA level were measured after 72 hr.Results The cultured cells grew linearly with optical density (OD) of 0.067 ± 0.021 after 2 days and 0.449 ± 0.113 afier 5 days.SOD activity was significantly lower in the three magnetic field intervention groups than in the control group.Under hypoxic culture conditions,in all the magnetic field intervention groups SOD activity increased at first and then deereased.Under normal culture conditions,MDA content was significantly higher in the 182.1 mT group than in the control group.In the other two groups it was significantly lower.Conclusion Magnetic field exposure can cause oxidative damage to pancreatic islet cells,at least rat cells in culture.Under hypoxic culture conditions a magnetic field can inhibit such damage.

Objective To establish neonate rate models induced by high-fat diet so as to explore the effect of high-fat diet before and during pregnancy on growth and serum insulin-like growth factor-1 (IGF-1) level in neonate offspring.Methods Forty female SD rats were divided into high-fat group and control group that were fed with 35％ high-fat diet and standard chow,respectively.After 8 weeks,8 rats from each group were euthanatized for liver pathology and the other female rats were mated with male rats and fed continuously with 35％ high-fat diet and standard chow throughout gestation,respectively.Offspring from both groups were studied within 24 hours after birth.Their birth weight and body length (from apex nasi to end of tail) were measured.Enzyme-linked immunosorbent assay was used to detect serum IGF-1 level.Serum biochemical indexes were tested by biochemical analyzer.Liver pathologic changes were observed under the light microscope.The expression of IGF-1 in liver samples was detected by Western blot.Results 1.Rats in the high-fat group showed a significant less birth weight and shorter body length compared with those in the control group(all P ＜ 0.05).2.The level of serum IGF-1 of offspring in the high-fat group decreased by 20.1％ in comparison to offspring in the control group,but there was no significant difference between the 2 groups (P ＞ 0.05) because of the limited sample sizes.3.The aspartate transaminase(AST) of offspring in the high-fat group increased compared with those from control group(P ＜ 0.05),while other serum biochemical indexes between the 2 groups showed no significant differences(all P ＞0.05).4.Fatty degeneration could be found in livers of both dams and offspring in the high-fat group under light microscope,while the samples of both dams and offspring from the control group all showed normal.5.The IGF-1 expression in livers of offspring from high-fat group increased in comparison to that from control group(P ＜ 0.05).Conclusions A maternal high-fat diet before and during pregnancy decreases the birth weight and body length of offspring in utero,which may be associated with the decreased IGF-1.However,the pathogenesis of decreased IGF-1 in this study can not been found.Further study is needed to clarify the mechanism of intrauterine growth retardation induced by high-fat diet.

Objective To investigate the feasibility of Antituberculotic?loaded bone cement (ATLBC) prepared by mix?ing the anti?TB drugs Rifampicin (RFP), Isoniazid (INH), Pyrazinamid (PZA), Moxifloxacin (MFX) with Palacos R PMMA bone cement in Total Joint Arthroplasty treatment for Joint Tuberculosis. Methods Forty grams of Palacos R bone cement powder without antibiotics was mixed with 1 or 2 grams of RFP, INH, PZA and MFX respectively. According to ISO 5833:2002 stan?dard, 8 groups of ATLBC standard test specimen were prepared as experiment group and Palacos R PMMA bone cement with?out antibiotics was prepared as control group. Physical properties (such as the average dough time, curing time, maximum tem?perature), mechanical strength (such as the compressive strength, the bending resistance strength, the modulus of elasticity) and the concentrations of eluant drug in different time points of ATLBC were detected. Results In RFP (1 g), RFP (2 g), INH (1 g) and INH (2 g) group, the average dough time and curing time were longer than those in control group, which exceeded the standard scope of ISO, while the average maximum temperature was significantly lower than that in control group. The INH ( 1 g) group and INH (2 g) group hardened after mixing for 14 days. The RFP (1 g) group and RFP (2 g) group hardened after mixing for 30 days. Twenty minutes after mixing and hardening, the compressive strength, bending resistance strength and modulus of elastic?ity were significantly lower than the specified values of ISO standard. The physical properties and mechanical strength in PZA ( 1 g) group, PZA (2 g) group, MFX (1 g) group, MFX (2 g) group and control group were in accordance with the specified values of ISO standard, and they hardened after 20 minutes. In RFP (1 g) group, RFP (2 g) group, INH (1 g) group, INH (2 g) group, PZA (1 g) group, PZA (2 g) group, MFX (1 g) group and MFX (2 g) group, the concentration of eluant could maintain for 3 days, 7 days, 90 days, 90 days, 45 days, 60 days, 60 days and 60 days respectively. Conclusion RFP and INH mixing with Palacos R PMMA bone cement can hinder the aggregation of bone cement so they are unsuitable for preparing ATLBC. PZA and MFX mixing with Palacos R PMMA bone cement do not affect the physical properties of bone cement, with excellent mechanical strength and elu?tion properties. Because the minimal inhibitory concentration of PZA is higher and its antimicrobial activity maintains shorter time, while MFX maintains longer time in antimicrobial activity, it's more suitable for the preparation of ATLBC.

Objective:Using a combination of 2D and 3D methods to evaluate the difference of lip changes between two maxillary anterior segmental osteotomy methods.Methods:30 patients who visited the Department of Orthognathic Surgery of Tianjin Stomatological Hospital from January 2015 to January 2017 with maxillary protrusion [8 males and 22 females, aged (25.0 ± 0.6) years old] were divided into two groups. One group was treated with cupar maxillary anterior segmental osteotomy. One group underwent modified method. 4 osseous tissue measurements, 8 soft tissue measurements and 7 lip measurements were collected in three-dimensional model, lateral cephalometric radiographs and frontal photos at one week before surgery and one year after surgery to observe the difference between the two groups in the lip area and calculate the retreat ratio of upper incisor to upper lip.Results:The wounds of all 30 patients were healed in one stage without infection and bone necrosis. There was no recurrence of the bone protrusion at 1 year after the operation. After treatment, the bone relationship was normal and the dental arch and occlusal curve were normal. The occlusion relationship and the lip-tooth relationship were good. The shape of the lower third of the face was significantly improved, and the relationship between nose, lips and chin was coordinated. The change of the horizontal position of the lip and the position of the neck of upper incisor tooth (UJ) had a significant positive correlation. In traditional group R= 0.768 and in modified group R= 0.771. The sella-nasion-A point decreased and the nasolabial angle increased in both groups. The difference was statistically significant before and after surgery ( P<0.05). The amount of retraction of the neck point and incision point of the upper incisor and the amount of retraction of the upper lip were significantly reduced. The difference before and after surgery was statistically significant ( P<0.05). There was an increase in the thickness of the upper lip before and after surgery, a decrease in the distance between the upper and lower lip, and a decrease in the distance between the upper incisor and the upper lip. The differences were statistically significant ( P<0.05) compared with those before surgery. The increase of upper lip height in the modified group was statistically significant ( P<0.05), but not in the traditional group. Upper lip height showed a statistically significant increase in the modified procedure group. The retreat ratios of the point Pro of incisor and the upper lip were 1.19∶1 and 0.81∶1 in traditional group and modified group. In frontal view, it was observed that the midline lip thickness became more significantly smaller although the limited change amount and the upper lip line tended to be M-type. Conclusions:These two surgical procedures have obvious effects on maxillary protrusion deformity. The change of the horizontal position of the lip and the position of the neck of upper incisor tooth (UJ) had a significant positive correlation. The lip height increased in modified group compared to traditional group.

Objective:Using a combination of 2D and 3D methods to evaluate the difference of lip changes between two maxillary anterior segmental osteotomy methods.Methods:30 patients who visited the Department of Orthognathic Surgery of Tianjin Stomatological Hospital from January 2015 to January 2017 with maxillary protrusion [8 males and 22 females, aged (25.0 ± 0.6) years old] were divided into two groups. One group was treated with cupar maxillary anterior segmental osteotomy. One group underwent modified method. 4 osseous tissue measurements, 8 soft tissue measurements and 7 lip measurements were collected in three-dimensional model, lateral cephalometric radiographs and frontal photos at one week before surgery and one year after surgery to observe the difference between the two groups in the lip area and calculate the retreat ratio of upper incisor to upper lip.Results:The wounds of all 30 patients were healed in one stage without infection and bone necrosis. There was no recurrence of the bone protrusion at 1 year after the operation. After treatment, the bone relationship was normal and the dental arch and occlusal curve were normal. The occlusion relationship and the lip-tooth relationship were good. The shape of the lower third of the face was significantly improved, and the relationship between nose, lips and chin was coordinated. The change of the horizontal position of the lip and the position of the neck of upper incisor tooth (UJ) had a significant positive correlation. In traditional group R= 0.768 and in modified group R= 0.771. The sella-nasion-A point decreased and the nasolabial angle increased in both groups. The difference was statistically significant before and after surgery ( P<0.05). The amount of retraction of the neck point and incision point of the upper incisor and the amount of retraction of the upper lip were significantly reduced. The difference before and after surgery was statistically significant ( P<0.05). There was an increase in the thickness of the upper lip before and after surgery, a decrease in the distance between the upper and lower lip, and a decrease in the distance between the upper incisor and the upper lip. The differences were statistically significant ( P<0.05) compared with those before surgery. The increase of upper lip height in the modified group was statistically significant ( P<0.05), but not in the traditional group. Upper lip height showed a statistically significant increase in the modified procedure group. The retreat ratios of the point Pro of incisor and the upper lip were 1.19∶1 and 0.81∶1 in traditional group and modified group. In frontal view, it was observed that the midline lip thickness became more significantly smaller although the limited change amount and the upper lip line tended to be M-type. Conclusions:These two surgical procedures have obvious effects on maxillary protrusion deformity. The change of the horizontal position of the lip and the position of the neck of upper incisor tooth (UJ) had a significant positive correlation. The lip height increased in modified group compared to traditional group.

Objective To explore the influence of field tracing defect situational simulation teaching on new nurses' critical thinking. Methods From 2015 to 2016, the new nurses from a general Class Ⅲ hospital were selected by cluster sampling method. The 2015 new nurses were assigned as control group (n=75), and the 2016 new nurses were assigned as observation group (n=83). Traditional situational simulation teaching was adopted in the control group, while the field tracing defect situational simulation teaching was applied in the observation group. The effects of training was evaluated with Chinese Version of Critical Thingking Disposition Inventory. Results The results of critical thinking ability of both the groups increased after training (P<0.05). The result of the observation group was (265.87±44.19), which was higher than that of the control group (243.39±37.25), and the difference was statistical significant (t=3.438, P=0.001). Conclusions The field tracing defect situational simulation teaching can effectively improve new nurses' critical thinking ability.