1.Bioinformatics analysis of genes related to the progress of cervical intraepithelial neoplasia
Chinese Journal of Clinical Oncology 2016;43(19):840-844
Objective:To investigate the potential genes associated with cervical intraepithelial neoplasia (CIN) progression through mi-croarray expression profiling data analysis and bioinformatics approaches. Methods:mRNA expression microarray data related to CIN progression were screened from GEO database for the first time. They were re-analyzed by bioinformatics analysis. Results: Two mRNA expression microarray datasets were obtained from the GEO database. Pathway enrichment analysis of the common differen-tially expressed genes identified 3 signaling pathways associated with CIN progression, including Wnt, Endocytosis, and Vibrio cholerae infection. Fourteen differentially expressed genes were also identified. Biological annotation and text mining showed that 3 genes were directly related to CIN progression, and 9 other genes were associated with tumor progression and recurrence. GeneMania tool analysis demonstrated the protein interaction network formed between all the differentially expressed genes and the 24 reported genes. CCND2 and TGFBR2 formed direct interaction with many reported genes. Conclusion:Three signaling pathways and 14 differen-tially expressed genes were associated with CIN progression, as indicated by microarray data analysis results.
2.Clinical significance of MMP-2、MMP-9 expression in gastrointestinal stromal tumor
Xiaobo YU ; Ruizheng MIAO ; Yanming JIANG
Chinese Journal of Current Advances in General Surgery 1999;0(02):-
Objectives:To evaluate the clinical significance of MMP-2,MMP-9 expression in gastrointestinal stromal tumor.Methods:54 of GIST were studied for MMP-2,MMP-9 by immunohistochemical staining,and the relationship of MMP-2,MMP-9 with each pathological factor were analyzed.Results:The positive expression of MMP-2,MMP-9 was 85.2%(46/54) ,83.3%(43/54) respectively.The expression of MMP-2,MMP-9 increased significantly according to the malignant increasing(P
3.Dose distribution of low dose and half-body irradiation in simulated manikin
Yaqin QU ; Yongfeng PIAO ; Xin JIANG ; Yanming YANG ; Zhiming CHEN
Journal of Jilin University(Medicine Edition) 2006;0(03):-
Objective To study the security of low dose and half-body irradiation by 60Co ?-rays as a new method of clinical radiotherapy.Methods The simulated manikin was used to simulate human body and two radiation modalities of facing and backing on radioactive source were adopted.Half-body irradiation was done by 60Co ?-rays with doses of 9,10 and 11 cGy.The exposure dose of every layer and important target organs in the simulated manikin were detected,and the security of low dose and half-body irradiation as a therapeutic method was evaluated.Results The exposure dose of every layer and sensitive organs were all within safety margin,when simulated manikin facing or backing on the radioactive source was irradiated by 60Co ?-rays with doses of 9,10 and 11 cGy.Further,the exposure dose of sensitive organs in the simulated manikin backing on the radioactive source was lower than that in those facing the radioacive source.Conclusion The method of low dose and half-body irradiation as a radiotherapeutic method is safe and feasible and the radiation modality of backing on the radioactive source is more safe.
4.The clinical study on prophylactic radiation therapy on sacral lymph nodes after radical resection of cervical cancer
Yaqin QU ; Yubao HE ; Xin JIANG ; Xiaojing JIA ; Yanming YANG ; Shaohua WANG
Tumor 2010;(1):57-61
Objective:To study whether sacral lymph nodes should be included in the target volume delineation for those patients with early (stageⅠB-ⅡA) uterus cervix cancer during postoperative radiotherapy. Methods:Forty-six patients with early uterus cervix cancer were given postoperative three dimensional conformal radiation therapy (3D-CRT) in our department for one month after radical resection. The patients were randomly divided into two groups. The sacral lymph nodes were not included in the target volume delineation in the treatment group. But they were delineated in control group. All the patents had no radiotherapy-related contraindications and signed the consent agreement. The patients were followed up. The local controlling rate and the incidence and degree of radioactive proctitis were compared between the two groups. Results:The local controlling rate of the two groups had no significant difference(t=0.000, P=1.000). The doses received by the 5% and 95% volume of the rectum(V_5, V_95), the average dose, and the minimum dose had significant difference between the two groups(t_(V5)=2.169, P_(V5)=0.041; t_(V95)=4.036, P_(V95)=0.001;t_(mean)=2.236, P_(mean)=0.036; t_(min)=2.265, P_(min=0.034), but the maximum dose received by the rectum had no obvious difference (t_(max)=0.518, P_(max)=0.610). The incidence of radioactive proctitis had significant difference between the two groups(t=2.174, P=0.190). Conclusion:For the early uterus cervix cancer patients who have recurrent risk after radical surgery, sacral lymph nodes should not be included in the delineation of target volume during 3D-CRT in order to decrease the incidence of radioactive proctitis.
5.Mutation analysis of the PTCH1 gene in a pedigree with nevoid basal cell carcinoma syndrome
Di GAO ; Ershun HUANG ; Tingting SUN ; Huimin YAN ; Yanming YOU ; Wei JIANG
Chinese Journal of Dermatology 2014;47(12):860-863
Objective To analyze mutations in the PTCH1 gene in a pedigree with nevoid basal cell carcinoma syndrome (NBCCS).Methods Blood samples were collected from a 58-year-old male proband with NBCCS (Ⅱ 5),his brothers (Ⅱ 1 and Ⅱ 3) and son (Ⅲ4),and 50 unrelated healthy human controls.DNA was extracted from these blood samples.PCR and direct DNA sequencing were performed to determine mutation sites in the PTCH1 gene.According to the mutation sites,allele-specific oligonucleotide primers were designed and used to confirm the pathogenic mutations in this pedigree through PCR.Results A nonsense mutation (c.2137C),which leads to the substitution of CAG by TAG with the generation of a premature termination codon (Q714X),was identified in exon 14 in one allele of the PTCH1 gene in the proband and his son,but in none of the healthy human controls.Conclusion The nonsense mutation (c.2137C > T) in the PTCH1 gene may be a specific mutation causing the clinical symptoms in the patient with NBCCS.
6.A retrospective study after treatment of proximal humeral fractures with locking plates
Junzheng DONG ; Haibo SHEN ; Yanming LI ; Limin WANG ; Yiming ZHU ; Chunyan JIANG
International Journal of Surgery 2010;37(8):521-523
Objective The purpose of our study was to evaluate the treatment of proximal humeral fractures with AO Philos plate. Methods From June 2007 to May 2009, 29 cases of proximal humeral fractures were fixed with AO Philos plate. According to Neer classification, 11 cases were two-part fractures, 16 cases three-part fractures and 2 cases four-part fractures. Results Twenty-nine cases were followed 9-16 months (averaging 11.3 months). The results were evaluated with Neer's evaluation system: 13 patients were graded as excellent,12 as good, 4 as fair. Conclusion AO Philos plate is less invasive comparing with traditional method. It can provide solid fixation, permit early functional rehabilitation and lead to a compromising clinical result.
7.Expression of cyclooxygenase-2 and vascular endothelial growth factor in gastrointestinal stromal tumor and its clinical significance
Ruizheng MIAO ; Yueping LI ; Xiaobo YU ; Jianfeng LI ; Naiqing LIU ; Yanming JIANG
Chinese Journal of General Surgery 2001;0(09):-
Objective To investigate the relationship of expression of cyclooxygenase-2(COX-2) and vascular endothelial growth factor(VEGF) with the growth pattern,malignant degree,metastasis and prognosis of gastrointestinal stromal tumor(GIST).Methods The expression of COX-2 and VEGF was examined with immunohistochemistry from paraffin-embedded tissue sections of 54 patients with GIST,and special attention was given to analyze the relationship between COX-2、VEGF expressions and clinicopathologic factors of the neoplasm.Results The expression of COX-2 and VEGF was significantly higher in malignant GIST than in benign and potentially malignant GIST(P
8.The protective effects of angiotensin Ⅱ receptor blocker irbesartan on kidney function in diabetic rats
Yanming CHEN ; Longyi ZENG ; Weikang WU ; Guochao ZHANG ; Boquan JIANG ; Hanchua LUO
Chinese Journal of Pathophysiology 2000;0(11):-
AIM: To investigate the effects and mechanisms of irbesartan,one of the angiotensin Ⅱreceptor blockers,on kidney function in diabetic rats. METHODS: Forty adult male Wistar rats were randomly divided into four groups: control group,diabetes group,irbesartan group and captopril group. At the end of 12 weeks,the rats were sacrificed. Urine volume,body weight,kidney weight/body weight,plasma,glucose,glycosylated hemoglobin (HbA_1c),urinary ?_2-microglobulin (?_2-MG) excretion,urinary albumin excretion rate (UAR),creatinine clearance (Ccr) were measured. Nitric oxide (NO) and endothelin-1 (ET-1) levels in plasma,urinary and renal tissues were determined. RESULTS: Urine volume,kidney weight/body weight,plasma glucose,HbA1C,UAR,Ccr,urinary ?_2-MG excretion,NO and ET-1 levels of urinary,blood and renal tissue in diabetic rats were significantly higher than those of normal controls ( P
9.Clinical research of Yiqi Sanju Formula in treating central obese men at high risk of metabolic syndrome
Chunyan HE ; Wenjian WANG ; Bin LI ; Desheng XU ; Weihua CHEN ; Jiang YING ; Yanming HE
Journal of Integrative Medicine 2007;5(3):263-7
OBJECTIVE: To observe the therapeutic effects of Yiqi Sanju Formula (YQSJF), a compound Chinese herbal medicine, on central obese men at high risk of metabolic syndrome (MS). METHODS: Compared with 30 healthy male volunteers, 45 central obese men were separated randomly into two groups and received the interventions with YQSJF and placebo respectively for 10 weeks. Baseline characteristics, insulin resistance, inflammation cytokines and plasminogen activator inhibitor-1 (PAI-1) and tissue plasminogen activator (t-PA) were evaluated before and after treatment. RESULTS: The score of homeostatic model assessment for insulin resistance (HOMA-IR) and the levels of C reactive protein (CRP), free fatty acid (FFA) and PAI-1 in obese men were higher than those in the control group, while t-PA was lower. After treatment, compared with placebo group, body mass index, waist, and waist-to-hip ratio were decreased significantly in subjects who received YQSJF (P<0.01). The score of HOMA-IR and the levels of CRP, FFA and PAI-1 were decreased significantly, and the level of t-PA was increased significantly (P<0.01). CONCLUSION: YQSJF can reduce obesity and insulin resistance in central obese men at high risk of MS and improve inflammation and fibrinolysis, which indicates that it can reduce the risk of atherosclerosis.
10.Performance evaluation of 20 blood glucose meters for home use testing
Junhan WANG ; Gang HUANG ; Rong LU ; Hong JIANG ; Min FENG ; Wei YAO ; Yanming CHEN ; Hongyun HU
International Journal of Laboratory Medicine 2015;(6):808-810
Objective To evaluate the precision and accuracy of blood glucose meter(BGM ) for community home use testing . Methods According to the In Vitro Diagnostic Test Systems‐Requirements for Blood Glucose Monitoring Systems for Self‐Testing in Managing Diabetes Mellitus in DIN EN ISO15197 :2013 .Capillary blood and venous blood were tested by BGM and the laborato‐ry biochemical analyzer respectively .The measurement results of each BGM were compared with the results of the biochemical ana‐lyzer for conducting the bias analysis .Results In evaluated 20 BGM ,none of them met the requirements of ISO15197 :2013(when blood glucose concentrations < 5 .5 mmol/L ,the bias in 95% of detection results is within the range of ± 0 .83 mmol/L ;when blood glucose concentrations ≥ 5 .5 mmol/L ,the bias in 95% of detection results is within the range of ± 15% .Only 11 imported BGM (55% ) met the state standard .Conclusion The bias of 20 BGM ranged - 28 .7% - 3 .8% ,with the average bias of - 12 .2% . These detection results will bring the large risk of therapy in the diabetic patients .The regular and standardized evaluation of BGM performance may ensure the quality of blood glucose self - monitoring .