AIM　The purpose is to investigate the Anti-inflammatory mechanism of Biota orientalis. METHODS　Using rat leukocytes and rabbit platelets as experimental material the biosynthesis of leukotriene, 12-hydroxyheptadecatrienoic acid(12-HHT) and 12-hydroxyeicosatetraenoic acid(12-HETE) in the cells was determined with HPLC. RESULTS　Biota orientalis was shown to inhibit the biosynthesis of leukotriene B4(LTB4) and 5-hydroxyeicosatetraenoic acid(5-HETE) with IC50 of 0.40 and 0.41　mg/ml crude herb, respectively. It was also displayed to inhibit biosynthesis of 12-HHT in the platelets. CONCLUSION　Biota orientalis leaves contain anti-inflammatory components and the anti-inflammatory mechanism was related with inhibiting metabolism of arachidonic acid.

There is strong relationship between self-efficacy and health promotion.And the researches about applying self-efficacy to promoting health behavior of the older adults increase as aging.This paper reviewed the self-efficacy and its relationship with health-promoting behavior of older adults.

AIM To investigate the anti-inflammatory eff ec t of total flavonoids isolated from Platychadus orientalis and its mechanism . METHODS The inflammatory models in mice and swelling of rats hind paws were induced by dimethylbenzene and carrageenan respectivel y. The biosynthesis of leukotriene and ?-glucuronidase release in the cells we re measured by HPLC and fluorescence spectrophotometer respectively. RES ULTS Total flavonoids isolated from Platychadus orientalis remarkably inhibited the ear edema induced by dimethylbenzene in mice at the dose of 12.5 ～50.0 mg?kg -1 and the swelling of rats hind paws induced by carrageenan at the dose of 25.0～50.0 mg?kg -1 . Total flavonoids at final concentrati on of 5.0～125.0 mg?L -1 inhibited 5-HETE and LTB 4 synthesis by 30.5%～ 87.3% and 27.8%～84.3% respectively. In comparision with controls, Total flavono ids at final concentration of 5.0～45.0 mg?L -1 inhibited ?-glucuronidas e release by 13.4%～32.2%. CONCLUSION Total flavonoids isolated f rom Platychadus orientalis has an anti-inflammatory effect which may be rel ated to inhibiting 5-HETE and LTB 4 production and ?-glucuronidase release.

Objective To investigate the main risk factors influencing the pre-hospital delay of patients with acute ischemic stroke (AIS) for preventing methods.Methods A retrieval was done across the epidemiologic studies on pre-hospital delay of AIS patients during 2004 to 2015 using the key words of ischemic stroke,prehospital delay,treatment delay.According to the inclusion and exclusion criteria,the literature was collected and the literature quality was assessed.The data were extracted for meta-analysis by RevMan software.Results Sixteen epidemiologic studies were included.Totally,there were 2,966 cases of prehospital delay and 2,468 cases of non-delay.The pooled OR values and 95％ CI by multivariate analysis were as follows:the degree of awareness of stroke was 0.5 (0.3,0.82),emergency medical service (EMS) was 0.49 (0.29,0.85),National Institute of Health Stroke scale (NHISS) score 0.58 (0.36,0.95),outpatient visit as first visit 4.28 (1.44,12.74),distance 0.76 (0.61,0.95),medical history of ischemic stroke 0.37 (0.18,0.79) (with significant differences in OR value,P＜0.05).Conclusions The protective factors for pre-hospital delay of acute ischemic stroke patients include:strengthened awareness of stroke,effective EMS,high NHISS score,short distance,medical history of ischemic stroke.The main risk factor is that their outpatient visit is their first visit for medical treatment.Therefore,we need to enhance the health education to the patients and their families about awareness of stroke,improve the use of EMS and build complete and efficient green path for the stroke patient.

The incidence of venous thromboembolism after spinal cord injury is high, and its diagnosis and prevention received much attention in clinical research in recent years. Because of the special characteristics, the prevention of venous thrombosis should be combined with basic prevention, physical prevention and drug prevention. According to the different situations of venous thromboembolism in patients with spinal cord injury, the prevention methods need to adjust to carry out multidisciplinary comprehensive treatment.

Objective To evaluate the values of visual inspection with acetic acid( VIA) and human papillomavirus( HPV) and thinprep cytology test( TCT) in the screening of cervical cancer.Methods VIA,HPV test and TCT in 946 women was conducted.Cervical biopsy or endocenical curettage( ECC) was performed in VIA positive women or HPV positive women or TCT positive women. The pathologic outcome was as the gold standard,and the out come more and equal to cervical intraepithelial neoplasia( CIN) Ⅱ were identified as positive.Results There was no significant difference among three groups in sensitivity rate( 73.l％,96.2,88.5％ ) and negative predictive value ( 99. 1％ ,99.9％ ,99.7％ ) ( all P ＞ 0.05 ).The specificity rate (97.5％ ) and positive predictive value ( 50.O％ ) of TCT was better than those of HPV ( 93.5％,29.4％ ),and HPV was better than those of VIA( 87.0％,13.7％ ),and the difference had statistifical significance among three groups ( x2 =71.34,26.00,22.17,8.28,all P ＜ 0.05 ).Conclusion Although the rate of missed diagnosis and misdiagnosis rate was higher than that of HPV and TCT,but VIA seems to be appropriate methods in the screening of cervical cancer owing to its low price.

Objective To explore the effect of intravenous infusion flow rebuilt on the patients’safety and nursing quality in intravenous infusion center.Methods Risk safety factors about intravenous infusion process in intravenous infusion center were analyzed and the work flow were rebuilt and optimized?The satisfaction rate,the reception time and the disinfection time for tourniquets were compared before and after work flow rebuilt.Results After work flow rebuilt,the satisfaction rate raised from 92?5%to 94?5%?There were significant differences among the reception time and the disinfection time for tourniquets before and after work flow rebuilt (all P<0?05)?After work flow rebuilt,both the reception time and the time for dealing with tourniquets are shorter than before. Conclusions The rebuilt work flow can make nurses work efficiently and enhance the patients’nursing quality?

Objective To explore the effect of multiple-formed nursing teaching ward-round.Methods Trainings and practices about muhiple-formed nursing teaching ward-round were executed to clinical instruction teachers.Results 95.9%of nursing students were satisfied with the quality of nursing teaching ward-round.After training ang practices,the evaluation to the nursing teaching ward-round and the scores of theoretic and operation examination of nursing students raised significantly(P＜0.05).Conclusions The trainings and practices improve the teachers' ability of utilizing multiple-formed nursing teaching ward-round,which not only enhance the students' study initiative,but also improve the query of nursing teaching teaching ward-round.

Objective To study TCM pattem rules of primary hyperlipemia and its relation with sex,age,body mass index(BMI).Methods A total of 1 20 patients with primary hyperlipemia were divided into 5 groups by TCM syndrome differentiation:56 patients in a group of the turbid-phlegm stagnation syndrome,35 patients in a group of yang deficiency of spleen and kidney syndrome,13 patients in a group of yin deficiency of liver and kidney,7 patients in a group of hyperactivity of yang due to yin deficiency group,and 9 patients in a group of qi stagnation and blood stasis syndrome.Statistic analysis was made on factors that may affect these syndromes,and the correlations were discussed.Results Different age showed a closer relation to the onset of primary hyperlipemia than other factors(P＜0.01):sex,clinical classification,and accompanied diseases had no relation with the five-type syndromes(P＜0.01).Conclusion TCM syndromes had a certain relation with the age of patients with primary hyperlipemia.

Aim To study the effect of resveratrol on the expression of FAK and the level of phosphorylated FAK, and to investigate its possible mechanism of resveratrol on the metastasis-associated ability of human highly metastatic ovarian carcinoma HO-8910PM cells line. Methods MTT assay was used to examine cytotoxicity of resveratrol on HO-8910PM cells after 24 h treatment; The expression of FAK protein and the level of phosphorylated FAK were assessed by Western blotting analysis. Results MTT assay showed that resveratrol had weak cytotoxicity on HO-8910PM cells after 24 h treatment, its IC50 value was 163.40?2.48 ?mol?L-1. The expression of FAK and the level of phosphorylated FAK decreased obviously in HO-8910PM cells treated with 25~100 ?mol?L-1 resveratrol for 24 h. Conclusion The mechanism of resveratrol inhibiting the metastasis-associated ability of human highly metastatic ovarian carcinoma HO-8910PM cells line involves the expression of FAK and the level of phosphorylated FAK.