Ocular ischemic syndrome (OIS) is a disease seen in cardiology,ophthalmology,neurology,and neurosurgery,which can lead to brain and ocular symptoms induced by carotid artery obstruction or stenosis.In local and general manifestation,ocular symptoms usually appear first.Ocular symptoms show the pre-warning effect of other ischemic damage.Ophthalmologists should pay attention to the clinical manifestation and damages of OIS.The establishment of multidisciplinary diagnosis and treatment patterns for OIS is a pivotal issue for several disciplines.

Mammalian gonadotropin-releasing hormone(termed GnRHⅠ) is a decapeptide that plays key role in the process of reproduction.In addition to its well-known endocrine function, it has become evident that GnRHⅠ and the second GnRH subtype(termed GnRHⅡ) are potentially important autocrine and/or paracrine regulators in placental trophoblast cells.The effects of GnRHⅠ and GnRHⅡ on trophoblastic cell invasion were investigated in human choriocarcinoma cell line(JEG-3) by the methods of invasion assay, RNA interference, Western blot and Real-time PCR.The data revealed that exogenous native peptide of these two forms of GnRH significantly induced the expression of typeⅠ GnRH receptor(GnRHRⅠ) in JEG-3 cells.The specific siRNA for GnRHR Ⅰ was capable of blocking the invasion-promoting effect of GnRHⅠ, but did not influence the effect of GnRHⅡ.The data suggested that the two hormones may be elicited by distinct receptors.Interference of the activities of ERK and JNK was capable of attenuating GnRH Ⅰ and Ⅱ-induced MMP-2 expression and trophoblast invasion, indicating that activation of ERK and JNK are required in both GnRH Ⅰ and GnRH Ⅱ-mediated MMP-2 production and invasion-promoting effect in human trophoblastic cells.

Objective To study the relationship between red cell distribution width (RDW ) and short-term outcomes in acute coronary syndrome (ACS) patients .Methods A totoal of 1654 ACS patients were divided into RDW ≤12 .1% group(n=419) ,RDW=12 .2% -12 .8% group(n=364) ,RDW=12 .9% -13 .2% group (n= 463) and RDW ≥13 .3% group (n= 408) .Their bio-chemical indicators and incidence of 1-month cardiac death ,heart failure ,recurrent myocardiac in-farction were compared .Relationship between RDW and 1-month adverse cardiac events was as-sessed .Results The incidence of cardiac death ,heart failure ,recurrent myocardiac infarction in-creased with the increasing RDW .Logistic regression analysis showed that RDW ,BNP>100 ng/L and LVEF< 40% were the independent risk factors for 1-month cardiac death in ACS patients (OR=2 .116 ,95% C I:1 .427-3 .137 ,P=0 .000;OR=3 .510 ,95% C I:1 .221 -10 .093 ,P=0 .020 ;OR= 4 .149 ,95% CI:2 .001 -8 .602 ,P= 0 .000 ) ,and that RDW ,age> 65 years ,diabetes and LVEF<40% were the independent risk factors for 1-month heart failure and recurrent myocardi-ac infarction in ACS patients (OR= 2 .134 ,95% CI:1 .602 -2 .844 ,P= 0 .000 ,OR= 2 .010 , 95% C I:1 .135 -3 .560 ,P=0 .017 ;OR=2 .279 ,95% C I:1 .345 -3 .862 ,P= 0 .002 ;OR=5 .009 , 95% CI:2.694-9 .316 ,P=0 .000) .Conclusion RDW is an independent risk factor for the short-term outcomes in ACS patients .

Objective To investigate changes in serum bilirubin and internal environment,etc.of neonatal hyperbilirubinemia that before and after the treatment of peripherally arteriovenous synchronous exchange transfusion. Methods In the strict monitoring of vital signs,hyperbilirubinemia peripheral vessels exchange transfusion was applied in five cases of neonatal,and the serum bilirubin,routine blood test,electrolytes before and after the exchange transfusion were detected.Results By comparing the data before and after the exchange transfusion,the total bilirubin and indirect bilirubin decreased obviously,respectively[(414.02 ±56.04)μmol/L,(225.72 ±53.54)μmol/L,t =5.433 P =0.01,(381.82 ±44.68)μmol/L,(199.22 ±57.37 )μmol/L,t =5.615 P =0.01 ],the difference was statistically significant,and also the platelet count were decreased obviously,respectively [(199.00 ± 42.01)×109 /L,(102.00 ±17.72)×109 /L,t =4.758,P =0.001],the difference was statistically significant.After the exchange transfusion,white blood cells,hemoglobin,potassium,sodium,chloride,calcium,glucose,blood pressure, heart rate and blood PH value had decreased or increased slightly,there was no significant difference P >0.05.Cere-bral palsy and other complications did not occur after 12 months of follow -up of the five cases.Conclusion The outer peripheral arteriovenous synchronous exchange transfusion therapy for neonatal severe hyperbilirubinemia can quickly reduce serum bilirubin,and has less impact on the body environment,it is an important and effective salvage treatment of neonatal hyperbilirubinemia,thereby reducing damage to the nervous system of bilirubin.

HIV /AIDS related lymphoma (ARL)are a group of heterogeneity of neoplasms,and they have poor prognosis.The factors of pathogenesis elucidated in recent years include immune injury caused by HIV,EB virus infection and genetic changes.There are four pathological types of ARL,including diffuse large B cell lymphoma,Burkitt lymphoma,plasmablastic lymphoma and primary effusion lymphoma.Morphological characteristics,immunophenotype markers and clinical data should be combined to make diagnosis and differen-tial diagnosis,which will facilitate timely treatment and improve prognosis.

[Objective]To compare long-term conversion between fresh and cryopreserved osteochondral allografts in order to further provide theoretical foundation for treatment of articular cartilage defect using osteochondral allografts. [Methods]Articular cartilage defect models of 5 mm in diameter and depth were established for 32 New Zealand rabbit’s knees and treated by fresh and cryopreserved osteochondral allografts. Allografts were harvested and assessed by observing and analysing proteoglycan content,chondrocyte viability and ultrastructure of articular cartilage using Alcianblue staining and BCECF-AM /PI fluorescent staining,respectively,at 12 and 18 months postoperatively.[Results]Proteoglycan content and percentage of viable chondrocytes of osteochondral allografts were obviously decreased.Results of fresh osteochondral allografts were significantly better than those of cryopreserved osteochondral allografts at different time points. Ultrastructure of chondrocytes demonstrated degenerative changes.[Conclusion]In treatment of artificial articular cartilage defect ,serious degenerative changes were found for both fresh and cryopreserved osteochondral allografts at a long period,suggesting that fresh,especially cryopreserved osteochondral allografts are not feasible to treat articular cartilage defect.

Objective Ganglouyuchuang lotion was used to dress the wound for Anal fistula in Crohn's disease, control inflammation and promote wound healing.This paper studied the extraction techonology of ganglouyuchuang lotion.Methods Four factors of ganglouyuchuang lotion, including volume of water, extraction time, extraction times and liquid ratio, were studied by the orthogonal test, and three levels were selected for each factor.The content of sodium danshensu, the active component contained in Chinese herbal medicine, was regarded as evaluating indicator, and the content of Danshensu Sodium was determined by HPLC.The water extraction and alcohol precipitation technology of ganglouyuchuang lotion was optimized according to the results of measurement.Results The optimum extraction technology of ganglouyuchuang lotion was as follows: four herbs, including Salvia miltiorrhiza, Radix Astragali, Radix sanguisorbae and Senecio were added with8 times amount of water overnight and decocted 3 times with 2 h, 1.5 h, and 1.5 h respectively, and then the extraction was concentrated to the ratio of herbs and concentrate of 1∶1.5.The results showed that the contents of Danshensu Sodium from the three examples were 0.520, 0.498, and 0.521 mg/mL, and the RSD were 0.34%, 0.41%, and 0.29%.Conclusion The optimum extraction technology is feasible and applicable for the preparation of ganglouyuchuang lotion.

In the present study, electron microscopic and cytochemical localization of succinic dehydrogenase (SDH) of the tumor-like growth tissue in the Tiger-head goldfish (Carassius aurtus) were attempted. The SDH was demonstrated according to the modified method described by Kerpel-Fronius and Hajos (1968). The main results are as follows:When the goldfish were twenty-months old, there was not only a highly active positive reaction of SDH on the periphery of jejune vesicle, but also on the mitochondria, cristae and cytoplasm membrane in the cells of the hyperplasitic tissue. At the same time, there were much crystal formation because cell disintegration. When the Tiger-head goldfish were ten and thirty-months old, the active reaction of SDH was not so strong as in the corresponding positions of cytodeme as When it was twenty-months old. The crystal formation were lesser than the others. But the control groups displayed the negative reaction of SDH in the corresponding positions.

Objective To investigate the impact of hepatic steatosis on the outcome of adefovir therapy in chronic hepatitis B patients with HBeAg positive. Methods The medical records of 154 patients who were diagnosed with CHB positive for hepatitis B e antigen (HBeAg) and received adefovir therapy for 24 weeks between January, 2011 and December, 2013 were analyzed retrospectively. Baseline status and virologic response, biochemical response and serology response between patient with (n = 68) and without (n = 86) steatosis who were proven by ultrasound. Both groups were given oral adefovir dipivoxil 10 mg/d for 24 weeks , recovery rate in ALT/AST, negative conversion rate of HBV DNA and HBeAg were recorded and compared. Results Recovery rate in ALT/AST in observation group was 67.7%, which is lower than control group (83.7%), and the difference is significant(P < 0.05). Negative conversion rate of HBV DNA and HBeAg in control group is 81.4%and 83.7%, which is significantly higher than observation group (whose values are 58.8% and 44.1%, P < 0.05). Conclusion Hepatic steatosis has significant impact on the outcome of adefovir treatment.

Objective To explore the application method and effect of mind map in practice of clinical nursing courses among nursing undergraduates. Methods In the teaching of clinical nursing courses such asAdult Nursing and Maternal and Perinatal, 70 nursing undergraduates received the teaching method by using mind map in clinical practice. A self-designed questionnaire was used and a symposium was held after clinical practice, to collect and file the feedback on the effect of using mind map teaching from 70 nursing students. Results The score of the attitude and effectiveness towards mind map was high ranged from (3.69±0.73) points to (4.29±0.57) points. Conclusions The mind map is an effective clinical teaching method, applying mind map in nursing clinical practice can improve the teaching effect. The students show positive attitudes to the teaching method.