Objective To investigate the influence of Buyanghuanwu (BYHW) Decoction on cerebral infarct volume and pathological changes after cerebral ischemia and reperfusion. Methods The model of focal cerebral ischemia and reperfusion was made by thread embolism method. Healthy male SD rats were divided randomly into sham-operated group, model group and BYHW Decoction group. Infarct volume was detected by triphenyltetrazolium chloride (TTC) staining and pathological changes were observed microscopically in HE stained sections. Results In BYHW Decoction group, infarct volume was significantly smaller than that in model group (P

Objective To investigate the protective effects of astragaloside Ⅳ on blood-brain barrier(BBB) and influence on expression of ZO-1 protein after cerebral ischemia and reperfusion in rats.Methods 72 SD rats were randomly divided into:sham operation group,model group and two astragaloside Ⅳ therapy groups [group A(10 mg/kg,group B(20 mg/kg)].Method of Evans blue leakage and water content in the brain tissue were used to investigate the permeability of the BBB,and method of immunohistochemistry combined with image analysis was used to measure the expression of ZO-1 protein in microvessels.Results Compared with those in the model group,Evans blue leakage and water content were less and the number of ZO-1 possitive vessels was increased in the two astragaloside Ⅳ therapy groups.There were statistical differences between therapy groups and model group(all P

Objective To investigate the cervical human papillomavirus (HPV) infection, genotypes and related high risk factors in 459 women of annual health examination. Methods A total of 459 cervical cytology specimens were de-tected with nest PCR and pyrosequencing methods. Questionnaires were collected simultaneously and the risk factors of HPV infection were analyzed. Results The detection rate of HPV-positive samples was 17.9%. Seven kinds of high-risk genotypes of HPV were found. The detection of HPV 16 was the most common (9.8%), followed by HPV58 (7.0%) and HPV18 (5.2%). The detection rates of simplex infection, dual infections and multiple infections were 9.6%, 4.8%and 1.5%respectively. The risk factors for HPV infection included alcohol consumption,income <3 000 yuan/ month, sexual part-ners>1, frequency of sexual activity>4 times/month and cervical erosion (P<0.05). Conclusion HPV DNA genotyping can provide important reference for HPV screening at early time and the application of HPV vaccines, which also provide sig-nificant evidence for the prevention, diagnosis and treatment of cervical cancer at early stage for the high risk population.

Objective: To observe the effects of tetramethylpyrazine on the content of tumor necrosis factor?(TNF?) and the activity of myeloperoxidase(MPO) after cerebral ischemia/reperfusion in rats,and to investigate its mechanism of protective effects on cerebral ischemia/reperfusion.Methods: Thirtysix male SD rats were randomly divided into shamoperated group,model group and(tetramethylpyrazine) group.The thread with embolus was(inserted) in the cerebral(middle) artery in shamoperated group,but the artery was not(embolized).The cerebral ischemia/reperfusion models of rats were established in model group and(tetramethylpyrazine) group,while in the latter group,33 mg/kg of tetramethylpyrazine was(injected)(intraperitoneally) 30 minutes before ischemia.The methods of radioimmunoassay and pectrophotometry were used respectively to detected the content of TNF? and the activity of MPO in brain tissues of rats.(Results): Compared with those in shamoperated group,the content of TNF?((0.601?0.089) ?g/L vs.(1.576?0.153) ?g/L) and the activity of MPO((0.026?0.008) U/g vs.(0.409?0.044) U/g) in brain tissues(increased) markedly in model group(both P

Objective To investigate the effects of UVB irradiation and calciu m at different concentrations on the expression of pemphigus antigens by culture d human keratinocytes. Methods Human keratinocyte cultures were treated with eit her 2 mmol/L calcium added to the serum free media, or exposure to UVB irradiat ion. Immunofluorescence staining was performed with sera from patients with pemp higus vulgaris (PV) or pemphigus foliaceus (PF) as the first antigen. Extracts f rom both epidermis and keratinocytes were run on SDS-PAGE according to Laemmli ′s method and transferred to nitrocellulose membrane for immunoblot with PV and PF sera. Results Specific staining with PV sera was always detectable on kerati nocyte culture by immunofluorescence assay with or without high concentrations o f calcium while PF antigen was detected on stratified cells only. However, expos ure to UVB irradiation could not evoke keratinocyte culture express PF antigen. The reactivities were found at 160 kd band with PF sera while at both 160 kd and 130 kd bands with PV sera. Conclusions Monolayer or stratified keratinocytes ca n produce PV antigen, by increasing the concentration of calcium in the culture media, the human cultured kera tinocytes can be induced to stratify and express PFA. Human keratinocytes can not express PF antigen after exposure to UVB in intro.

Objective To study the action and mechanism of staphylococcal exfoliative toxin A(E-TA)on pemphigus foliaceus antigen(PFA)and pemphigus vulgaris antigen(PVA)expressed on cultured human keratinocytes.Methods Stratified human keratinocytes were incubated with ETA and then stained with sera from patients with pemphigus foliaceus or pemphigus vulgaris as the first antibodies and FITC-la-beled sheep anti-human IgG as the second antibody.Total protein was harvested from the cells pretreated with ETA and run on SDS-PAGE for Western blot with the same antibodies.Simultaneously,supernatants of the keratinocytes before and after ETA treatment were collected for detection of the levels of IL-1?,IL-6with ELISA kits.The caseinolytic activities of the supernatants were tested by spectrometry in which casein was used as a non-specific substrate.Results Down-expression of PFA was shown after ETA treatment while no change of PVA expression was found.The high intensity and continuous linear appearance of fluo-rescent staining before ETA treatment became weak and discontinuous after ETA treatment,which were re-covered gradually in24hours.The degradation of proteins recognized by PF sera after ETV treatment was revealed by Western blot.The decreasing tendency of IL-1?concentration was found in the supernatants of cell culture after ETA treatment,but IL-6level was too low to be detected.Increased caseinolytic activities were found in the supernatants,and declined36hours after ETA treatment.Conclusions ETA acts on PFA expressed on keratinocytes in vitro,which is reversible along with withdrawal of ETA.The mechanism of E-TA act on PFA may be related to proteolytic action instead of promoting cytokine secretion.

Objective: To explore the correlation between the expression of C-terminal tensin-like protein (CTEN) and the prognosis of hepatocellular carcinoma (HCC). Methods: Using immunohistochemistry, we detected CTEN protein level in samples of primary lesion and adjacent non-tumor lesion collected from 240 patients with HCC. The relationship between CTEN expression and clinicopathology, 5 year recurrent-free survival, or overall survival was evaluated by Chi-square test, Kaplan-Meier, or Cox regression analysis. Results: High CTEN expression was detected in 55% of hepatocellular carcinoma tissues and 20%of adjacent carcinoma tissues (P<0.001). CTEN expression was positively correlated with tumor diameter (P=0.022), venous invasion (P=0.007) or TNM stages (P=0.022). Five-year recurrence-free survival time (P<0.001) and overall survival time (P<0.001) in patients with high CTEN expression were signiifcantly less than those in patients with low CTEN expression. Multivariate Cox regression analysis revealed that the CTEN expression was an independent prognostic marker for HCC (all P<0.05). Conclusion: CTEN protein may play a role in the genesis and development of HCC, and it can function as a prognostic marker.

Shepp-Logan head phantom is the classical model of simulation computing in Computerized Tomography. This paper proposes a new method--considering 3D Shepp-Logan head phantom as the basic reference model to carry on simulation experiment and algorithms performance evaluating in three-dimension medical image reconstruction field. The paper introduces the design and the implementation of 3D Shepp-Logan head phantom as well the computing of projection data at first, and then describes the simulation process of the three-dimension medical image. The numerical experiment gives the three-dimension medical image reconstruction simulations based on 3D Shepp- Logan head phantom. The result of this experiment indicates the accuracy of model computing and the feasibility of our this method.
Algorithms ; Computer Simulation ; Head ; diagnostic imaging ; Humans ; Image Processing, Computer-Assisted ; methods ; Imaging, Three-Dimensional ; Models, Anatomic ; Phantoms, Imaging ; Tomography, X-Ray Computed ; instrumentation

Objective To investigate the effect of alprostadil in the treatment of interventional angiography in elderly patients with coronary heart disease after contrast nephropathy prevention.Methods From February 1,2014 to January 31,2017,60 elderly patients with coronary heart disease with interventional therapy in Yuncheng Central Hospital of Shanxi Province were divided into two groups,with 30 cases in each group.The control group received hydration therapy,the observation group was given alprostadil combined with hydration therapy.The biochemical index changes before surgery and three days after surgery and incidence of contrast nephropathy were compared between the two groups.Results Before surgery and three days after surgery,the tumor necrosis factor alpha,beta 2 microglobulin, urea and creatinine in the two groups were not changed significantly.Three days after surgery,24h urine protein, superoxide dismutase,glutathione peroxidase,interleukin －6 and C reactive protein levels in the two groups were increased,the increase amplitude of the observation group was smaller.Three days after surgery,the five indicators of the observation group were (185.54 ±86.47)mg,(2.01±1.32)mg/L,(6.18 ±2.13)g/L,(135.56 ±41.58)ng/L, (1.21±1.05 )mg/L,respectively,the differences were ststistically significant between the two groups (t ＝1.21, 1.24,1.50,1.26,1.22;P＝0.03,0.03,0.04,0.03,0.04).Three days after surgery,the creatinine clearance rate of the observation group was (98.67 ±21.56)mL/min,which was higher than that of the control group(t＝2.71,P＝0.01).The incidence rate of contrast induced nephropathy in the observation group (3.33%)was lower than that in the control group(χ2＝5.19,P＝0.02).Conclusion Alprostadil can effectively prevent contrast nephropathy in elderly patients with coronary heart disease after interventional therapy.

Objective:To construct the "3+2" counterpart cut-through sectional training nursing major un-dergraduate curriculum system, which is oriented by vocational competence. Methods: The preliminary draft of"3+2" nursing undergraduate curriculum setting was established base on the literature review and expert group in-terview, and the 25 experts was conducted two rounds of expert questionnaire consultation using Delphi method. Results:Experts' opinions tended to be consistent after two rounds of consultation, the expert authority coefficient was 0 . 92 , the coordination coefficient of Kendall was 0 . 44 in the second round of expert consultation and finally established 5 curriculum groups, including total 28 courses of public elementary courses, professional basic cour-ses, professional core courses, professional oriented courses and centralized practice courses. Conclusion: It should construct the"3+2" counterpart cut-through nursing major undergraduate curriculum system, which is o-riented by vocational competence, and achieve effective connection between the knowledge structure and the quality of the nursing students, in order to provide the reference for perfecting the curriculum system of vocational educa-tion in our country.