AIM: To establish the content determination method of matrine and phillyrin in Jieyinjing Suppositories(Radix Sophorae Flavescentis,Fructus Forsythiae,Cortex Phellodendri Chinensis,Radix Stemonae,etc.) by HPLC. METHODS: HPLC was performed to determine matrine on ODS column,the mobile phase consisting of methanole-0.02% triethylamine(55∶45) and detection wavelength at 220 nm and the phillyrin of mobile phase consisting of acetonitrile-water(25∶75) and its detection wavelength at 277 nm. RESULTS: The linearity of matrine was in the range of 9.8 ?g?mL~(-1)-49.0 ?g?mL~(-1)(r=0.999 96),and the average recovery was(99.48%,) RSD=1.49%;The linearity of phillyrin was in the range of 5.4 ?g?mL~(-1)-54.0 ?g?mL~(-1)(r=(0.999 9),) and the average recovery was 100.07%,RSD=1.34%. CONCLUSION: This method is simple,sensitive and accurate,and available for control of Jieyinjing Suppositories.

Objective To establish a method for the content determination of naringin in Bogu Pill by HPLC. Methods At room temperature and by using ultrasonic extraction, HPLC was performed to determine naringin content on ODS chromatographic column.The mobile phase consisted of a mixture of acetonitrile and 8 % acetic acid (15 ∶ 85)and detection wavelength was at 283 nm.Results The linearity of naringin was in the range of 5.2 ? g / mL～ 31.2 ? g / mL (r=0.9999) and the average recovery was 98.72 % , RSD=1.55 % . Conclusion This method was simple, sensitive and accurate, and can be used for the quality control of Bogu Pill.

Objective To investigate the effect of puerarin on preeclampsia-like rat placental tissue hypoxia inducible factor (hypoxia-inducible factor,HIF)-1αexpression and study the mechanism of protective effects in rats.Methods 60 SD rats were randomly divided into control group,drugs intervention groups(low-dose,middle-dose,high dose)and model group.The rats in drugs intervention groups and model group were adopted endotoxin for induction of preeclampsia model.On the basis of the model group,low,medium and high dose groups were given 20.0,40.0,60.0 mg/kg puerarin by intraperitoneal injection,respectively.The blood pressure of tail artery,urine protein concentration were observed at different points and the expression of HIF-1αwere detected by PT-RCT in each group.Results The systolic blood pressure ,urine protein and HIF-1αprotein in model group were higher than control group(P<0.05 );serum NO concentration was lower than control group (P<0.05 );after the puerarin intervention,systolic blood pressure and urine protein concentration in drugs intervention groups were lower than model group (P<0.05 ),plasma NO concentration was higher than model group (P<0.05 )and HIF-1αprotein was lower than model group (P<0.05 );the placenta weight,weight and body length of newborn rats in drug intervention group were higher than model group (P<0.05 ).Conclusion Puerarin can reduce systolic blood pressure and urine protein levels,promote the level of NO in plasma and reduce HIF-1αlevel in placenta of rats.Puerarin can offer protection to rats with preeclampsia by decreasing the expression of HIF-1αin placental.

Neurobiology is a cross subject,in which"Language and Attention"is an impor-tant chapter as a hotspot involved with physiology,psychology,anatomy and biology.Inorder to im-prove the teaching efficiency,it is necessary to apply the techniques such as arranging the content logically,giving prominence to the emphases and breaking through the difficulty,using interactive teaching method and collocating the assistant method rationally. Through this way,it is easier for the students to get interested in this chapter and grasped the content.

Objective To investigate changes in serum bilirubin and internal environment,etc.of neonatal hyperbilirubinemia that before and after the treatment of peripherally arteriovenous synchronous exchange transfusion. Methods In the strict monitoring of vital signs,hyperbilirubinemia peripheral vessels exchange transfusion was applied in five cases of neonatal,and the serum bilirubin,routine blood test,electrolytes before and after the exchange transfusion were detected.Results By comparing the data before and after the exchange transfusion,the total bilirubin and indirect bilirubin decreased obviously,respectively[(414.02 ±56.04)μmol/L,(225.72 ±53.54)μmol/L,t =5.433 P =0.01,(381.82 ±44.68)μmol/L,(199.22 ±57.37 )μmol/L,t =5.615 P =0.01 ],the difference was statistically significant,and also the platelet count were decreased obviously,respectively [(199.00 ± 42.01)×109 /L,(102.00 ±17.72)×109 /L,t =4.758,P =0.001],the difference was statistically significant.After the exchange transfusion,white blood cells,hemoglobin,potassium,sodium,chloride,calcium,glucose,blood pressure, heart rate and blood PH value had decreased or increased slightly,there was no significant difference P >0.05.Cere-bral palsy and other complications did not occur after 12 months of follow -up of the five cases.Conclusion The outer peripheral arteriovenous synchronous exchange transfusion therapy for neonatal severe hyperbilirubinemia can quickly reduce serum bilirubin,and has less impact on the body environment,it is an important and effective salvage treatment of neonatal hyperbilirubinemia,thereby reducing damage to the nervous system of bilirubin.

Objective To explore the clinical effect of systemic nursing intervention on constipation after coronary heart disease. Methods 145 patients were randomly divided into intervention and control group. Patients in invention group were given systemic nursing intervention, including diet, exercise, health education and so on. The patients in control group were treated with cathartic.Results There was significant difference between intervention and control group in incidence of constipation or related complication(P<0.05). Conclusion Systemic nursing intervention is helpful to reduce constipation and prevent the related complication.

Objective To investigate the protective effects of astragaloside Ⅳ on blood-brain barrier(BBB) and influence on expression of ZO-1 protein after cerebral ischemia and reperfusion in rats.Methods 72 SD rats were randomly divided into:sham operation group,model group and two astragaloside Ⅳ therapy groups [group A(10 mg/kg,group B(20 mg/kg)].Method of Evans blue leakage and water content in the brain tissue were used to investigate the permeability of the BBB,and method of immunohistochemistry combined with image analysis was used to measure the expression of ZO-1 protein in microvessels.Results Compared with those in the model group,Evans blue leakage and water content were less and the number of ZO-1 possitive vessels was increased in the two astragaloside Ⅳ therapy groups.There were statistical differences between therapy groups and model group(all P

The importance of information resources organization under E-Learning environment was analyzed ac-cording to the new features and technologies of E-Learning environment with the development trends in information resources organization summarized .

Cancer/testis(CT) antigens,of which more than 40 kinds have now been identified,are encoded by genes that are expressed normally in the human germ line,and are also expressed in various tumor types,including melanoma,and carcinomas of the bladder,lung and liver.These immunogenic proteins are being vigorously pursued as targets for therapeutic cancer vaccines.In this paper,the classification,expression and function of CT antigens are reviewed.

is of STC.Exogenous GDNF may improve the colon motor function by up-regulation of GDNF.