Objective To investigate the therapeutic effect and nursing ways of pulmonary surfactant in premature babies with respiratory distress syndrome. Methods From July 2008 to January 2011,63 cases of premature babies with respiratory distress syndrome were admitted to our department, pulmonary surfactant was dripped through trachea into these patients, the treatment effect after medication was observed. Results Among these premature babies, one case died of respiratory failure, one case died from pulmonary hemorrhage, sixty-one cases were cured, the cure rate was 96.8%. After treatment, the arterial blood pH, partial pressure of oxygen(PaO2) and oxygen saturation(SaO2) of the 61 patients was significantly higher than before treatment, the arterial blood partial pressure of carbon dioxide (PaCO2)、 total carbon dioxide (TCO2)、actual bicarbonate (AB)was significantly lower than before treatment. Conclusions Pulmonary surfactant combined with appropriate nursing ways is able to cure premature babies with respiratory distress syndrome effectively.

Objective To observe the clinical therapy of hand-foot-and-mouth disease, and to summarize the method of nursing intervention. Methods A retrospective analysis was carried out in 112 pediatric patients with hand-foot-and-mouth complicated with viral encephalitis disease of our hospital from February 2009 to May 2010. The method of nursing intervention during treatment was summarized. Results According to the incidence of pediatric patients, regardless of during or after treatment,various nursing interventions were taken. And ultimately, patients were all cured, after discharge they had no significant sequelae. Conclusions There are seasonal and age differences among pediatric patients,pertinent nursing intervention should be taken in order to make patients recovery early.

Objective To analyze the impact factors and their importance on academic reputation of medical research institutions in China.Methods Seventy medical researchers and 70 medical graduate students were selected by convenience sampling method.Brainstorming and expert consultation were applied to determine the 6 factors affecting the academic reputation of medical research institutions in China.A Face-to-face or e-mail interview was conducted using a designed questionnaire.Six affecting factors were nominated analyzed.Chi-square test was used to cross exam nominated rate and affecting factors;Bonferroni method was also applied to make paired comparisons.Results There is significant difference in nomination rates among 6 impact factors (P ＜ 0.001).Pairwise comparison showed that information obtained by academic exchange (70.49 ％) and the scientific understanding generated from research practice (67.21％) are top 2 impact factors to the medical research institutions;followed by all kinds of ranking list (44.26％),and the celebrity effect (42.62％).Conclusions Scientific communication and research practice are two most important factors affecting the academic reputation of medical research institutions in China.

Objective To investigate alexithymia and perceived stress in nursing staff.Methods Nurses who were 18 to 45 years old were selected with clustered sampling method and finished the Toronto Alexithymia Scale (TAS-20) and the Perceived Stress Scale (PSS).Results TAS total sore ranged from 25 to 84.The incidence of critical state group and alexithymia group was respectively 38.5％ and 26.0％.There was no significant difference of alexithymia in nursing staff with different ages,education background and marital status.The total score of TAS-20 was significantly higher in general nurses than in nursing cadres,and the score of alexithymia factor was significantly higher in senior nurses than in junior nurses and in-charge nurses.The perceived stress was rising in non-alexithymia group,the critical state group and alexithymia group and the scores had significant difference.Conclusions There is a high incidence of alexithymia in nursing staff and more stress is perceived in nursing staff with alxithymia.More attention should be paid to and more psychological intervention should be given to younger nurses.

0.05). Conclusion Comparedwith DSA and operation,there is excellent correlation among 3D contrast-enhanced moving-bed MRA,DSA and operation.MRA canaccurately and entirely assess occlusive disease of the arteries of the lower extremity.It is a ono-invasive,reliable and potential newtechnique.

Mink plasmacytosis, caused by Aleutian Mink Disease Virus (AMDV), poses a threat to the development of the animal fur industry. Neutralizing antibodies against AMDV may result in a persistent infection rather than providing protection for minks. To date,no specific methods to prevent or cure this disease have been developed. In order to eliminate mink plasmacytosis, antibody detection technology has been used globally as a dominant approach to screen for AMDV-positive minks. This paper introduces the classical technology, counterimmunoelectrophoresis and emerging technology in terms of AMDV antibody detection,and provides a glimpse into the future development of these technologies.
Aleutian Mink Disease ; diagnosis ; immunology ; virology ; Aleutian Mink Disease Virus ; immunology ; isolation & purification ; Animals ; Antibodies, Viral ; immunology ; Immunoassay ; instrumentation ; methods ; Mink

or physicians and specialists other than psychiatric professionals in general hospitals should be enhanced together with psychiatric consultation.

Objective: To explore the correlation between the expression of C-terminal tensin-like protein (CTEN) and the prognosis of hepatocellular carcinoma (HCC). Methods: Using immunohistochemistry, we detected CTEN protein level in samples of primary lesion and adjacent non-tumor lesion collected from 240 patients with HCC. The relationship between CTEN expression and clinicopathology, 5 year recurrent-free survival, or overall survival was evaluated by Chi-square test, Kaplan-Meier, or Cox regression analysis. Results: High CTEN expression was detected in 55% of hepatocellular carcinoma tissues and 20%of adjacent carcinoma tissues (P<0.001). CTEN expression was positively correlated with tumor diameter (P=0.022), venous invasion (P=0.007) or TNM stages (P=0.022). Five-year recurrence-free survival time (P<0.001) and overall survival time (P<0.001) in patients with high CTEN expression were signiifcantly less than those in patients with low CTEN expression. Multivariate Cox regression analysis revealed that the CTEN expression was an independent prognostic marker for HCC (all P<0.05). Conclusion: CTEN protein may play a role in the genesis and development of HCC, and it can function as a prognostic marker.

Objective To evaluate the influence of ultrasound-mediated contrast agent microbubbles carrying kallidinogenase targeted therapy on neurogenesis and angiogenesis after experimental acute cerebral infarction.Methods Kallidinogenase-loaded microbubbles were prepared using mechanical shaking method.Middle cerebral artery occlusion (MCAO) model was established in male Wistar rats by sutureoccluded method.MCAO rats (n =120) were randomly divided into 5 groups:ultrasound-mediated kallidinogenase-loaded microbubbles group (group 1),ultrasound-mediated microbubbles group (group 2),ultrasound-mediated saline group (group 3),kallidinogenase group (group 4),saline group (group 5).Medication was given through tail vein daily for 2-6 consecutive days starting 24 h after MCAO.Ultrasound groups (groups 1,2 and 3) were given 2 MHz pulse ultrasonic irradiation on the ischemia lateral skull for 10 min after injection.Cell proliferation was examined using 5'-bromo-2'-deoxyuridine (BrdU,50 mg/kg).Infarction volume and neurological function were evaluated on the 3rd,7th day after MCAO respectively.Doublecortin (DCX) + cells in the subventricular zone and laminin + in the peri-infarction region were observed at the same time.Results The number of DCX + cells in the subventricular zone (under 20 × ocular) of groups 1-5 was 251.8 ± 13.1,125.7 ± 11.6,130.2 ± 13.7,234.5 ± 12.4 and 123.7 ± 10.0 respectively.The percentage of laminin + cells in the peri-infarction region (× 10 objective) of groups 1-5 was 10.0％ ± 0.8％,5.2％ ± 0.7％,5.0％ ± 1.0％,8.0％ ± 1.8％ and 5.0％ ± 0.9％ respectively.The number of DCX + cells in the subventricular zone and laminin + cells in the peri-infarction region of the group 1 and the group 4 was significantly more on the 7th day after MCAO compared with those of the other three groups (DCX:t values of group 1 vs groups 2,3,5 were 17.88,17.17 and 18.16,all P ＜ 0.01 ; t values of group 4 vs groups 2,3,5 were 15.42,14.78 and 15.70,all P ＜0.01.Laminin:t values of group 1 vs groups 2,3,5 were 7.01,6.71 and 7.11,all P ＜ 0.01 ; t values of group 4 vs groups 2,3,5 were 4.23,3.94 and 4.33,all P ＜0.01).Moreover,the number of DCX+ cells in the subventricular zone (t =2.46,P ＜ 0.05) and laminin + cells in the peri-infarction region (t =2.78,P ＜ 0.05) of the group 1 was much more than those of the group 4.Neurologic scores on the 7th day of groups 1-5 were 1.00 (0.75,1.25),2.0 (2.00,3.00),2.0 (1.00,2.00),1.5 (0.75,2.00),2.0 (2.00,2.50).Compared with other four groups,group 1 showed better functional improvement after stroke (U values of group 1 vs groups 2-5 were 2.0,4.0,7.5 and 2.5,all P ＜ 0.05).While there was no significant difference in infarction volume among five groups at all the time points after MCAO.Conclusions Our study demonstrates ultrasound-mediated kallidinogenase-loaded contrast agent microbubbles targeted therapy promotes neuroblasts proliferation and vascular regeneration compared with mediated and non-medicine-loaded microbubbles therapy,which attributes to functional improvement after MCAO.Therefore,ultrasound-mediated ultrasound contrast agent microbubbles carrying drugs targeted therapy may have a perspective on ischemic stroke.

Objective To investigate clinical and radiological characteristics of reversible posterior leukoencephalopathy syndromes(RPLS) in preeclampsia/eclampsia and to summarize prognosis of patients and fetus in order to provide the basis for early diagnosis and early treatment.Methods Clinical and radiological characteristics, and treatments and outcomes of 33 pregnant women with RPLS in preeclampsia/eclampsia were analyzed retrospectively.All patients were treated in Guangzhou Medical Center for Critical Pregnant Women during January 2013 and July 2015.Results Clinical symptoms :23 of 33 patients were with headache, 19 cases with visual changes, 15 with seizures, and 12 cases with conscious or mental disorder besides 3 cases with nausea and vomiting, and 4 cases with chest tightness and shortness of breath symptoms.Imaging features: 33 cases were all checked with radiological examination in acute period, which suggests edema of white matter.It involved the most common parts of occipital lobe(24 cases), 13 cases in the parietal lobe,9 cases in temporal lobe and basal ganglia,and 6 cases in frontal lobe.Moreover, the common parts involving the brain stem occurred in 4 cases, and 2 cases of cerebellum.Clinical outcomes: 33 cases of pregnant women in prenatal emergency termination of pregnancy include 31 cases of cesarean section, and 1 case of natural delivery.After active treatment, the prognosis was good, except 1 case complicated with systemic lupus erythematosus (SLE) died, the rest were good.Fetal prognosis : stillbirths occurred in 8 cases, the rest were good.Conclusions The clinical presentations of RPLS in patients with eclampsia or preeclampsia are typical, including headache, epileptic seizures, visual changes, conscious, or mental disorder.The imaging features which involved the most common parts are occipital lobe and parietal lobe.The early diagnosis and timely intervention lead to a sound prognosis.