Objective To check the change of content of serum beta-apoliprotien(?-AP) and insulin(INS) in the patient with Alzheimer disease, and to reflect the effect of using different drug. Methods 60 cases of this disease and 30 cases of the control group were been detected by radioimmunoassay(RIA). Results The content of ?-AP in Alzheimer disease(AD) was obviously higher than that in control groups(P

Objective To investigate the changes of plasma IL-6 and IL-8 level and the inhibitory effects of preoperative administraction of bile salt or anti-endotoxin antibody against endotoxin in cases of obstructuve jaundice.Methods 50 cases with obstructive jaundice were randomized into OJT1 group taking bile salt orally,OJT2 group receiving intravenous anti-endotoxin antibody and OJ group receiving general treatment.The plasma levels of endotoxin(ET),interleukin-6(IL-6) and interleukin-8(IL-8) were determined in all the cases.Results The plasma ET level was significantly lower in the patients of OJT1 and OJT2 groups than in those of OJ group preoperatively and was further decreased postoperatively(P

Objective To explore the relationship between the expression of basic fibroblast growth factor (bFGF) and microvessel density(MVD)in non-small cell lung cancer.Methods The expression of bFGF and MVD were observed in 54 eases of NSCLC were detected with in situ hybrldization and immunohistochemical detection.Resuits The expression of bFGF and MVD was greater in adenecarcinomas than in squamous cell carcinomas of NSCLC (P＜0.05).The expression of bFGF was significantly different among the three groups of both squamous cell carcinomas and adenocarecnomas with varying differentiation (P＜0.05).There was hisher bFGF expression and greater MVD in NSCLC patients with regional lymplmode involvement and those with laterdistant metastasis(P＜0.05).Condusion bFGF may play an important role in tumor angiogenesis and lymphatic metastasis of human NSCLC,and detection of bFGF may be a good metastasis and prognostic predictors for human NSCLC.

Objective To investigate the prevalence and its major risk factors of NAFLD among enterprises employees of Rizhao region. Methods 8 259 employees aged over 18 were selected by multi-stage stratified random cluster sampling method in the enterprises of Rizhao region. The data were analyzed, such as height, weight, blood pressure,blood lipids,blood sugar,hepatitis series,and ECG,abdominal ultrasound,chest χ ray were conducted in the fasting state. The diagnosis of NAFLD was in line with diagnostic criteria adopted by China Institute of Liver Disease and alcoholic liver disease group, and the prevalence and major risk factors of NAFLD were investigated. Results The prevalence of NAFLD was 18. 1% (male 23.0% ,female 7. 6%;the ratio of male to female:3. 03:1) ,The prevalence of NAFLD reached a peak from 40 to 50 in male and after the age of 70 in female,and there was no statistically difference after the age of 50 between male and female. The close correlation was demonstrated by Logistic regression analysis between sex, hyperuricemia, HDLC, overweight or obesity, hyperglycemia, hypertriglyceridemia and NAFLD. Conclusion The prevalence of NAFLD was high, especially in older persons in enterprises employees of Rizhao region, and sex,hyperuricemia,HDLC,overweight or obesity,hyperglycemia and hypertriglyceridemia were closely associated with NAFLD.

It had some advantages when microwave induced hyperthermia were used to treat inferior femora and superior shinbone tumor.The device of circulation water,which could induce hypothermia during the treatment procedure needed to be used.Large amount of normal saline at the room temperature was perfused through this device,so the temperature of surrounding normal tissues and organs was reducd and the functions of nerve,vessel and knee were protected.It is safe and convenient measure that keep the operation go on smoothly.Summarized 54 cases of superior shinbone tumor that were used this device in the operation and experienced that it provided an effectiveness support for the operation.

It had some advantages when microwave induced hyperthermia were used to treat inferior femora and superior shinbone tumor.The device of circulation water,which could induce hypothermia during the treatment procedure needed to be used.Large amount of normal saline at the room temperature was perfused through this device,so the temperature of surrounding normal tissues and organs was reducd and the functions of nerve,vessel and knee were protected.It is safe and convenient measure that keep the operation go on smoothly.Summarized 54 cases of superior shinbone tumor that were used this device in the operation and experienced that it provided an effectiveness support for the operation.

Objective To investigate the effect of nifedipine on renal ischemia-reperfusion injury (I/R) in rats and the possible mechanism. Methods Forty-two male SD rats weighing 220-250 g were randomly divided into 3 groups with 14 animals in each group: sham operation group (group S); I/R group and I/R + nifedipine group (group N). The renal ischemia was induced by occlusion of bilateral renal arteries and veins for 45 min followed by reperfusion. In group S the renal arteries and veins were only exposed but not occluded. In group N, nifedipine 0.1 mg/kg was injected via the tail vein 15 min before ischemia and 15 min before reperfusion, while the equal volume of solvent was given in group I/R. The urine was collected to detect the N-acetyl-β-D-glucosaminide (NAG) activities at 6 and 24 h of reperfusion. Arterial blood samples were taken at 6 and 24 h reperfusion for determination of concentrations of serum creatinine (Cr), malondialdehyde (MDA) and NO. The rats were then sacrificed and the kidney was removed to detect the apoptotic cells in renal cortex and expression of heat shock protein 70 (HSP70) and endothelin- 1 (ET-1). Results The concentrations of serum Cr and MDA, NAG activity, expression of HSP70 and ET-1 and apoptosis rate were significantly increased in group I/R compared with group S (P ＜0.05). The concentrations of serum Cr and MDA, NAG activity, ET-1 expression and apoptosis rate were significantly decreased, while the serum NO concentration was significantly increased in group N compared with group I/R (P ＜ 0.05). Conclusion Nifedipine can attenuate renal I/R injury and the underlying mechanism may be related to the inhibition of ET-1 expression.

Objective To evaluate the reliability and validity of the Chinese-version Successful Aging Inventory (C-SAI).Methods The C-SAI was translated according to the Brislin translation model,and its reliability and validity was tested in 181 old adults.Results The content validity index for the scale (S-CVI/Ave) was 0.975.Five factors were extracted by principal components analysis which contributed 58.035％ to the variance.The Cronbach α and split-half reliability was respectively 0.832 and 0.871 for the total scale.Conclusions The C-SAI has good psychometric quality and can be used as a measurement tool for the successful aging.

Objective To provide a valid tool for assessment of the interpersonal climate of research teams by develo -ping a team interpersonal climate questionnaire .Methods Based on literature research and panel study , a pilot question-naire was constructed and handed out to a sample of 378 research graduate students in research teams .The analysis of va-lidity and reliability was conducted by SPSS 16.0 and Amos 17.0 for the questionnaire.Results The interpersonal climate in research teams is a two-facet concept , including mentor-mentee dyadic interpersonal relationship .The peer interpersonal relationship.The mentor-mentee dyadic interpersonal relationship consists of five dimensions: identification, support, developmental direction, individualized care , effectiveness of relationship , with the alpha coefficient ranging fron 0.931 to 0.959, while peer interpersonal relationship has only one dimension .Conclusion ①The interpersonal climate is a two-facet, six-dimension concept .②The self-developed interpersonal climate questionnaire achieves satisfactory validity and reliability .

Objective To screen for proteins interacting with novel gene AngRem104 and to identify the putative interaction of novel gene AngRem104 and Bardet-Bied1 syndrome 2 (BBS2) protein in mammalian cells. Methods The yeast strain AH109 was transformed with AngRem104pGBKT7/c-myc and yeast-mating was utilized to screen for interacting proteins with AngRem104 in pretransformed human kidney cDNA library. The human embryonic kidney (HEK 293T) cells were transformed with two recombined plasmids,AngRem104-pcDNA3.1/V5-His and BBS2-pCMV/c-myc. Mouse anti-human V5 monoclonal antibody and mouse anti-human c-myc monoclonal antibody were used in immunoprecipitation and immunoblot analysis, respectively. Results Seven proteins that interact with AngRem104, including BBS2 were identified. The AngRem104-V5 and the BBS2-c-myc fusion protein were detected respectively in the immunoprecipitation by anti-c-myc and anti-V5 antibody. Conclusion The novel gene AngRem104 may interact with BBS2 protein in mammalian cells,which provides insights as to the function exploration of novel gene AngRem104 and the pathogenesis investigation of Bardet-Biedl syndrome.