OBJECTIVE: To study the pharmacokinetics and bioavailability of pulse controlled-release tablets of ketoprofen(KRT) in dogs.METHODS: A total of 6 dogs were randomly assigned to receive KRT(trial group,n=3) or KP enteric-coated capsules(control group,n=3) 150 mg,then a crossover trial was conducted 1 week later.Plasma samples were taken at different time points and the plasma concentration of ketoprofen in dogs was determined by HPLC.The pharmacokinetic parameters of the two KP preparations were computed and the correlation between absorption percentage in vivo and release rate in vitro was compared.RESULTS: The pharmacokinetic parameters of KRT versus those of its control preparation were as follows: tmax:(5.00?0.41) vs.(3.75?0.00) h;Cmax:(15.40?1.94) vs.(20.73?1.72) ?g?mL-1;AUC0～t(101.33?17.49) vs.(93.21?25.03) ?g?h?mL-1.The relative bioavailability of the trial preparation was 108.71%.The test preparation showed a good correlation(r=0.987) between absorption percentage in vivo and release rate in vitro.CONCLUSIONS: The KRT and KP enteric-coated capsules were bioequivalent.

Objective To investigate the relationship between expression of eofilin-1 and in vivo implantation capacity of eutopic endometrium of endometriosis.Methods Eutopic endometrium of 20 cases with stage Ⅲ or Ⅳ endometriosis were obtained by laparoscopic or laparotomy surgery (endometriosis group) matched with 20 cases of eutopic endometrium from patients with cervical cancer in situ (control group) in Department of Obstetrics and Gynecology,ShengJing Hospital.All cases' eutopic endometrium were collected and injected into abdominal cavity of nude mice to establish endometriosis animal model, then the successful rate of animal model and volume of endometriosis lesion were calculated.The expression and positive rate of eofilin-1 protein were measured by western blot and immunohistochemistry staining.Results The mean volume of endometriosis lesions (2.38 ± 0.22)mm~3 in endometriosis group was significantly bigger than (0.36 ± 0.08) mm~3 in control group (P < 0.05).The successful rate of establishing endometriosis model was 95% (19/20) in endometriosis group and 5% (1/20)in control group, which reached statistical difference(P <0.05).The expression and positive rate of cofilin-1 protein in eutopic endometrium and the expression of cofilin-1 protein in endometriosis lesion of animal model were 0.82±0.06, 90% (18/20), 0.85 ± 0.03 and 0.21 ± 0.03, 20% (4/20), 0.22 ± 0.02 in control group, which reached statistical difference(P <0.05).The successful rate of establishing endometriosis model with positive cofilin-1 in endometrium 86% (19/22) was significantly higher than 6% (1/16) of model's endometrium with negative cofilin-1 expression(P <0.05).Conclusions Implanting capacity and cofilin-1 expression level of eutopic endometrium of endometriosis were more intensive than that of normal endometrium.High cofilin-1 expression was probably related with implanting capacity of eutopic endometrium of endometriosis.

Objective To explore the effect of simvastatin and atorvastatin on the level of p 27 protein in mesangial cells of rat under high glucose .Methods Cultured rat mesangial cells in vitro were divided into four groups :normal glucose(5 .6 mmol/L glu-cose) ,high glucose(30 mmol/L glucose) ,high glucose(30 mmol/L glucose) plus simvastatin(10 μmol/L) ,high glucose(30mmol/L glucose) plus atorvastatin(10 μmol/L) .After treatment for 24 ,48 ,72 120 hours ,the cells were collected .The protein level of p27 was detected by Western blotting and the mRNA level of p27 was detected by RT-PCR .Results Compared to the normal glucose group ,the levels of p27 protein and mRNA in the high glucose group had a significant increase(P<0 .05) .Compared to the high glucose group ,the level of p27 protein and mRNA in the high glucose plus simvastatin or atorvastatin group both had a significant decrease(P<0 .05) .Conclusion By modulating the expression of P27 in mesangial cells ,statins could become the independent lipid-decreasing drug to prevent kidney .

Objective To observe the distribution of the activated cerebral regions after acupuncture stimulation of Zhongzhu(SJ3) and Waiguan(SJ5) by functional magnetic resonance imaging(fMRI) technique.Methods A total of 8 cases of volunteer healthy subjects were recruited for this study.Before and during manipulation of the needle,cerebral images were sampled separately by fMRI scanner and analyzed by AFNI software.Results During manipulation of the needle inserted in the unilateral Zhongzhu(SJ3) and Wauguan(SJ5),the highly-activated cerebral structures were cingulated gyrus,temporal gyrus,cerebellum,occipital gyrus,etc.Conclusion The effect of acupuncture analgesia was probably produces by the interaction of multiple brain structures of functional connectivity rather than through the activation of a single brain region.

[Objective]To observe the efficacy of Jianlifang granules on anti-kinetic and anti-mental fatigue by experimental research.[Methods]SPF ICR mice were randomly divided into 5 groups:negative control group,Sanlejiang group,low-dose Jianlifang granules group,middle-dose Jianlifang granules group and high-dose Jianlifang granules group.The experiments of swimming,hypoxia tolerance test,pole-jump test,sedation,analgesia and improving the ability of learning and memory were used to test the ability of anti-fatigue in mice.[Results]The time of swimming,hypoxia tolerance,pole-jump and locomotor activity was remarkably prolonged in different doses of Jianlifang granules groups and Sanlejiang group compared with the negative control;the reaction time of licking foot was delayed and the pain threshold was increased;the correct rate of water maze test was significantly improved and the time of reaching destination was shortened(P

Unusual dTDP-sugars are key intermediate in many pathogenic bacteria. In this study, negative-ion electrospray tandem mass spectrometry (ESI-MS-MS) with collision-induced dissociation (CID) was used to study the fragmentation characteristics of six unusual nucleotide diphosphate sugars. The results indicated the major fragment of the six unusual nucleoside sugars observed in the ESI-MS-MS spectra resulted from cleavage of diphosphate moiety and their characteristic fragment ions at m/z 401, 383, and 321, correspond to [TDP-H] together with fragment ions resulting from the loss of water and phosphate moiety, respectively. Furthermore, 4-position substituted change of unusual sugar rings affected the stability of two important characteristic fragment ions of [glycosyl-1"-PO3](-) and [glycosyl-1"-P2O6](-).

Objective To evaluate the possibility of slow and rapid liquid nutrient loading test (LNLT) in the proximal gastric function assessment according to the results of gastric barostat testing.Methods From April to August in 2013,20 healthy volunteer were selected.The sensitivity of gastric,basic abdominal pressure,gastric sensing threshold,gastric discomfort and pain thresholds were examined by barostat.The average gastric volume after fasting was tested by barostat,which reflected gastric accommodation.And then 200 mL liquild meal was taken in five minutes by the volunteers.The difference value between the maximum gastric volume in 60 minutes after taking the meal minus the average gastric volume after fasting was used to evaluate the proximal gastric adapted diastolic function.In LNLT,volunteers drank 100 mL/min and 15 mL/min (rapid and slow) liquid meal.The threshold value of drinking volume,satiety drinking volume and maximum drinking volume were determined.Pearson correlation analyses were performed in the results of fast and slow LNLT,correlation between LNLT and sensitivity of gastric,gastric adapted diastolic function tested by barostat.Results The basic abdominal pressure,gastric sensing threshold,gastric discomfort and pain thresholds,gastric volume after fasting,maximum gastric volume and adapted diastolic volume was (6.8±1.2) mmHg (1 mmHg=0.133 kPa),(2.7±1.9) mmHg,(5.2±2.0) mmHg,(7.3-±-2.1) mmHg,(188.7-±-80.0) mL,(505.2±133.7) mL and (316.5±87.7) mL,respectively.The maximum gastric volume after the meal was at (9.8±3.3) min after meal.In rapid LNLT,the threshold value of drinking volume,satiety drinking volume and maximum drinking volume of volunteers was (209-±-96),(501±148) and (674±199) mL,respectively,and that in slow LNLT was (107 ± 43),(340 ± 134) and (524 ± 213) mL,respectively,there was correlation between rapid and slow LNLT (r=0.768,0.825 and 0.856,all P＜0.01).The maximum drinking volume of rapid and slow LNLT was at (8.6± 1.2) and (34.9±6.4) minutes after meal.Threshold value of drinking volume in the slow drinking test was positively correlated with the gastric sensing threshold (r=0.714,P＜0.01).Satiety drinking volume in the slow drinking test was correlated with gastric sensing threshold,gastric discomfort and pain thresholds (r=0.599,0.650 and 0.622,all P＜0.01).The proximal gastric adapted diastolic volume was related with slow satiety drinking volume,rapid satiety drinking volume,slow maximum drinking volume and rapid drinking volume (r＝0.543,0.636,0.527 and 0.493,all P＜0.05).Conclusions Threshold value of drinking volume and satiety drinking volume in the slow LNLT is more suitable for evaluating the sensitivity of stomach.Satiety drinking volume in the rapid LNLT is more suitable for the evaluating of gastric adapted diastolic function.

Second primary malignancies are more likely to occur in the multiple myeloma patients .Most of them are Acute myeloid leukemia ( AML) or Myelodysplastic syndrome ( MDS) .The incidence in male or those multiple myeloma patients who is less than 60 years old are more than female or those multiple myeloma patients who are bigger than 60 years old.In recent years,studies of multiple myeloma secondary to leukemia and other tumors are gradually increasing ,but the mechanism and treatment are still unclear .These diseases have the follow-ing characteristics such as progress rapidly ,high mortality rate and low complete remission rate after chemothera-py.

Health management of college students closely focuses on the health related problems of college students during their college life.From the perspective of life care,a school-based health management is carried out to conduct real concern and care for the health of college students,and establish a healthy life value for them.Not only the growth and development of these young people are promoted for their whole life,but also the overall health of the whole community is enhanced,so that the health management of college students has a remarkable significance for the health requirements and impact of the society as a whole.

Objective To assess language function in children with benign epilepsy with centrotemporal spikes in children(BECTS) and its association with the age of epilepsy onset.Methods We assessed language function in 63 children with BECTS and 30 age and sex matched controls by the subtest of CWISC.Results Children with BECTS performed significantly worse on all language tasks as compared to controls and overall better language function was positively correlated with older age of the child.Early age at seizure onset demonstrated significant negative correlation with language dysfunction,age below 6 years being related to the lowest performance scores(P ＜ 0.05).There was no relationship between the language function and the laterality of epileptic focus,seizure treatment status,or the duration of epilepsy(P ＞ 0.05).There was significant relationship between the language dysfunction and onset age of epilepsy (P ＜ 0.05).Conclusion Children with BECTS have language difficulties that are more pronounced in younger age group.Despite better language functioning in older children with BECTS,their verbal abilities remain inferior to those of children without epilepsy.Early age at seizure onset is a significant factor predicting worse language functioning in children with BECTS.