Objective To observe the distribution of the activated cerebral regions after acupuncture stimulation of Zhongzhu(SJ3) and Waiguan(SJ5) by functional magnetic resonance imaging(fMRI) technique.Methods A total of 8 cases of volunteer healthy subjects were recruited for this study.Before and during manipulation of the needle,cerebral images were sampled separately by fMRI scanner and analyzed by AFNI software.Results During manipulation of the needle inserted in the unilateral Zhongzhu(SJ3) and Wauguan(SJ5),the highly-activated cerebral structures were cingulated gyrus,temporal gyrus,cerebellum,occipital gyrus,etc.Conclusion The effect of acupuncture analgesia was probably produces by the interaction of multiple brain structures of functional connectivity rather than through the activation of a single brain region.

【Objective】 To observe the effect of acupuncture on immune organs(thymus and spleen) and T lymphocyte subtypes of CD3,CD4 and CD8 in rats with dysmenorrhea.【Methods】Thirty SD rats were randomized into normal control group,model group and electroacupunture(EA) group.Except the normal control group,rats in other groups received intraperitoneal injection of diethylstilbestrol and oxytocin to induce dysmenorrhea.EA group received EA on bilateral acupoints of Sanyinjiao and Guanyuan additionally.After 10-day treatment,body-stretching rate,the pathological changes of thymus and spleen,and blood CD3,CD4 and CD8 levels in the three groups were observed.【Results】In the model group,body-stretching rate was 100%,the pathological changes of thymus and spleen were obvious,and blood CD3 and CD4 levels were decreased as compared with the normal control group(P

Objective:To evaluate the effectiveness of multi-disciplinary treatment (MDT) for reducing carbapenem-resistant Enterobacteriaceae (CRE) infections in intensive care unit(ICU).Methods:Patients admitted in the emergency ICU (EICU) and neurosurgical ICU (NICU) of the First People’s Hospital of Lianyungang during January 2018 to December 2019 were enrolled in the study, which was analyzed by historical control study. For patients admitted in 2018, the conventional method was adopted for prevention and control of drug-resistant bacteria (control group, n=1, 076), and for patients admitted in 2019, the MDT was adopted for the prevention and control of CRE (intervention group, n=1, 237). Chi-square test was used to compare the incidence rate of CRE infection, CRE detection rate, rate of rational antibiotic use and compliance with implementation of prevention and control measures between two groups. Results:Compared to control group, the incidence rate of CRE infection in EICU and NICU decreased from 3.45% (14/406) and 3.58% (24/670) to 1.65% (9/547) and 2.32% (16/690) in intervention group, respectively; while the detection rate of CRE decreased from 66.21% (96/145) and 57.72% (86/149) to 41.11% (51/124) and 33.06% (40/121), the pathogens were mainly carbapenem-resistant Klebsiella pneumoniae (CRKP). The rational medication rate of carbapenem antibiotics was significantly increased from 65.00%(78/120) in 2018 to 92.73%(319/344) in 2019 ( χ2=55.382, P<0.05). In addition, the single room isolation rate, the rate of specialized nursing care, the cleaning and disinfection quality of bench surface and the special use rate of articles were also significantly improved( χ2=21.646, 18.116, 39.869 and 19.713, P<0.01). Conclusion:The establishment of multi-department collaborative management based on MDT can effectively improve the prevention and control effect of CRE in ICU and significantly reduce the prevalence of CRE infection.

Objectives: . The mitochondrial ribosomal protein L14 (MRPL14) is encoded by a nuclear gene and participates in mitochondrial protein translation. In this study, we aimed to investigate the role of MRPL14 in thyroid cancer. Methods: . We investigated the association between MRPL14 expression and clinicopathological features using The Cancer Genome Atlas (TCGA) and Chungnam National University Hospital (CNUH) databases. Functional studies of MRPL14, including proliferation, migration, invasion, mitochondrial oxidative phosphorylation and reactive oxygen species (ROS) production, were performed in papillary thyroid cancer (PTC) cell lines (B-CPAP and KTC-1). Results: . Based on the TCGA dataset, PTC tissues lost mitochondrial integrity and showed dysregulated expression of overall mitoribosomal proteins (MRPs) compared with normal thyroid tissues. Of 78 MRPs, MRPL14 was highly expressed in thyroid cancer tissues. MRPL14 overexpression was significantly associated with advanced tumor stage, extrathyroidal extension, and lymph node metastasis. MRPL14 increased cell proliferation of thyroid cancer and promoted cell migration via epithelial-mesenchymal transition-related proteins. Moreover, MRPL14 knockdown reduced the expression of oxidative phosphorylation complex IV (MTCO1) and increased the accumulation of ROS. Cotreatment with a ROS scavenger restored cell proliferation and migration, which had been reduced by MRPL14 knockdown, implying that ROS functions as a key regulator of the oncogenic effects of MRPL14 in thyroid cancer cells. Conclusion . Our findings indicate that MRPL14 may promote cell growth, migration, and invasion by modulating ROS in thyroid cancer cells.