Precise location of liver segments has great significance in diagnosis and treatment of hepatic disease. In recent years, medical imaging and three-dimensional imaging techniques provide more diagnostic information about the location of liver segments and research methods for finding more precise liver segment classification. The progresses of hepatic segment imaging were reviewed in this article.

Objective To investigate the expression of C-erbB-2 and anti-nuclear antigen Ki-67 in thyroid tumor, and its significance in pathologic diagnosis and prognosis the patient. Methods SP immunohistochemical technique was used to detect the expression of C-erbB-2 and Ki-67 in thyroid tissue of 90 cases of thyroid carcinoma and 20 cases of thyroid adenoma. Results High expression of both C-erbB-2 and Ki-67 in thyroid carcinoma, and their expression was weak in thyroid adenoma. The positive rate of C-erbB-2 expression in thyroid carcinoma was statistically higher than that in thyroid adenoma (48.9% vs 20.0%, P

Objective To evaluate the influences of artifical plasma substitutes on blood coagulation and fibrinoglysisMethods Forty adult patients, ASA grade Ⅰ-Ⅱ, scheduled for selective surgery ,were randomly allocated to receiving intravenous infusion of hydroxyethylh starch(HES group), polygeline (Haemaccel,HAE group), succinylated gelatin (Gelofusine,GEL group), or normal saline (control group) at the rate of 20ml?kg -1?h -1in 60 min ,respectively, with 10 cases in each groupThe venous blood samples were taken before and 1h following the infusion to determine platelet count(PLC),platelet agglutgtination test(PAG), platelet factor 3 availability test(PF 3at), activated partial thromboplastin time(APTT), prothrombin time(PT), thrombin time(TT), fibrinogen(FIB), tissue plasminogen(t-PA) and plasminogen activator inhibitor(PAI:A)Results In HAE group PAG decreased significantly, and PT was prolonged markedly after the infusion(P005)In GEL group only FIB reduced obviously after the infusion ,additionally was remarkably lower than that in control group(P

Objective To investigate the relationship between apoptcsis, expressions of VEGF and clinicopathological characteris- tics, and prognosis in esophageal squamous cell carcinoma (ESCC). Methods Sixty-one surgical specimens of primary esophageal squa- mous cell carcinomas were examined for VEGF by immunohistochemical staining (S-P). Apoptcsis was determined by TUNEL (TdT media- ting dUTP-biotin nick end-labeling) method. Clinicopathologic features were examined by histopathology. The prognostic impacts of these pa- rameters were analyzed by univariate and survival analysis. Results AI and VEGF were well correlated with differentiation, TNM stage. Lower tumor differentiation and higher TNM stage were related to decreasing AI and VEGF. In addition, VEGF in the groups of invasion be- yond muscularis and lymph node metastasis is significant higher than that in invasion reaching muacularis and no lymph node metastasis (P <0.01). But there were no significant correlation between AI and invasion( P>0.05). The simple-factor analysis results showed that the decrease of AI, VEGF, lymph node metastases, lower tumor differentiation, and invasion reaching muscularis were related to decrease of sur- vival rate. However, multivariate Cox analysis demonstrated that only AI and VEGF were the significant prognostic factors. Conclusions Apoptosis and angiagenesis participate in ESCC and promote its growth. VEGF is related to angiogenesis of ESCC. The increase of VEGF may promote invasion and lymph node metastasis. AI and VEGF are significant prognostic factors in ESCC.

Objective To observe the effect of lactoferrin supplementation combined with standard triple therapy on eradication rate of Helicobacter pylori (H.pylori) and its adverse reaction in children. Methods A total of 90 children who were diagnosed with H.pylori infection from January 2012 to January 2013 and had never received eradicative treatment were ran-domly divided into two groups. Forty-five children in control group received the standard triple therapy and another 45 children in observation group received lactoferrin supplementation combined with standard triple therapy. Adverse reaction during the treatment was recorded and eradication rate of H.pylori at 4 weeks after treatment was observed. Results The eradication rate of H.pylori in observation group (91.11%, 41/45) was higher than that in control group (73.33%, 33/45) and the adverse reaction rate in observation group (4.44%, 2/45) was lower than that in control group (20.00%, 9/45).The differences were significant (P<0.05). Conclusions Lactoferrin supplementation combined with standard triple therapy can increase H.pylori eradication rate and reduce the occurrence of adverse reaction.

Objective To explore the value of Gd-EOB-DTPA enhanced magnetic resonance imaging (MRI) in the quantitative evaluation of liver function.Methods Between June 2012 and June 2013,we prospectively included 8 patients with cirrhosis and 6 patients with primary hepatic carcinoma (PHC).The Child-Pugh classification,indocyanine green retention rate at 15 minutes (ICGR15),and Primovist enhanced MR imaging were performed in all the patients.Using the deconvolution method to calculate hepatic extraction fraction (HEF),the correlations between HEF and Child-Pugh and ICGR15 were analyzed.Results For cirrhosis,there was a significant correlation between the HEF and the ICGR15 by the Pearson correlation coefficient (r =-0.971,P ＜ 0.05).For hepatocarcinoma,there was also a significant correlation (r =-0.855,P ＜ 0.05).The average HEF of PHC was 21.80％ (14.89％ ～ 35.90％).Conclusions The Gd-EOB-DTPA enhanced MRI accurately evaluated liver function quantitatively.It may be used for patients with PHC for preoperative examination.

Objective:To explore the influence ofDan Shou Tang to fetal loss induced by APA.Methods:One hundred of10-week pregnant SD rats were divided randomly into two groups, every group were divided randomly into nourishing kidney and promoting blood flow group A, nourishing kidney group B, promoting blood flow group C, group D-APS model group and group E--blank control group.From the first day ofpregnancy, the rats in group A were given Dan Shou Tang through intragastric administration;the rats in group B were given formula to nourish kidney and rats in group C were given formula to promote blood flow, while group D and group E, as the control were given the corresponding physiological brine through intragastric administration.Then on the eighth and the twelfth day ofpregnancy, all rats ofgroup A, B, C and D were given multi-site subcutaneous injecting ofpurified ACA-IgG or LA-IgG with a dosage of15mg/ml, fats ofgroup E were injected the corresponding physiological brine.On the 15th day ofpregnancy, the rats were killed for samples.Results:Compared with APS model group, fetal absorptivity, ACA and APTT level were dramatically decreased in group A, B and C(P

Objective To investigate the relation between serum adiponectin and coronary artery calcification score (CACS), and find the risk factors for CACS in patients with chronic kidney disease (CKD). Methods Twenty-nine patients with 3-5 stage CKD were selected. The serum adiponectin was measured by enzyme linked immunosorbent assay. The heart was scanned by 64-row spiral CT, and the CACS was calculated. The blood calcium, phosphorus, intact parathyroid hormone, total cholesterol, low density lipoprotein cholesterol, albumin, urea nitrogen, creatinine, uric acid and high sensitive C reactive protein levels were measured, and the calcium-phosphorus product and estimation glomerular filtration rate were calculated. Results In 29 patients with CKD, 24 cases (83%) had coronary artery calcification with different degree (CACS>0 score), and the average CACS was 508 (0-3 363) scores. There were statistical differences in systolic blood pressure, urea nitrogen and estimation glomerular filtration rate between CKD patients with CACS≥100 scores (15 cases) and CKD patients with CACS<100 scores (14 cases):(146.00± 13.00) mmHg (1 mmHg=0.133 kPa) vs. (123.00±9.00) mmHg, (15.44±8.36) mmol/L vs. (9.71±2.52) mmol/L and (21.77 ±11.81) ml/ (min·1.73 m2) vs. (38.71 ±11.56) ml/ (min·1.73 m2), P<0.01 or <0.05. Pearson correlation analysis results showed that CACS had positive correlation with systolic blood pressure, creatinine and uric acid, and negative correlation with albumin and estimation glomerular filtration rate. Mult-stepwise regression analysis results showed that systolic blood pressure and estimation glomerular filtration rate were the independent risk factors of CACS. Conclusions The patients with 3-5 stage CKD have severe coronary artery calcification. The systolic blood pressure and estimation glomerular filtration rate are the independent risk factors of coronary artery calcification.

Objective To study the effect and mechanism of indomethacin(IN) on protein maps of human colon cancer cell line (HCT116). Methods Proteome profiles of HCT116 in IN treated and control group were separated by immobilized pH gradient based two dimensional gel electrophoresis (2 DE). Differential expression of protein and proteome analysis were identified and analyzed by silver scan using Image Scanner and ImageMaster software. Peptide mass fingerprint (PMF) was based on matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI TOF MS) and database searching. Results Clear background, well resolution and reproducible 2 DE patterns of HCT116 cells were acquired in IN treated and control group. The protein spots and match rate in control group and IN treated group were 1299?55 (94.2%) and 1208?47 (91.0%) . There were totally 45 differential protein spots, of which the expression of protein in IN treated group was down regulated in 34 spots and up regulated in 9 spots, while 2 spots of protein was expressed only in control group. Twelve differential proteins were identified by PMF, and HCT116 cells apoptosis was through BfL 1 signaling pathway. Conclusion IN can induce HCT116 cells apoptosis though BfL 1 signaling pathway.