Objective To study curative efficacy of Pramipexole in treatment of Parkinson's Disease and its effects on Serum C-reactionprotein (CRP), cystatin C, brain derived neurotrophic factor(BDNF) and oxidative stress.Methods 100 patients of Parkinson's Disease who received therapy from February 2014 to April 2016 in our hospital were selected as research objects were randomLy divided into control group and observation group, 50 patients in each group.The control group was treated with Dobasidate tablets;The observation group was given pramipexole tablets on the basis of the control group.Then the clinical effects and adverse reactions were compared.CRP, Cystatin C, BDNF and oxidative stress were measured. Results After treatment, the total effective rate of the observation group [(90.00% (45/50)] was significantly higher than the control group[74.00%(37/50)] (P<0.05);The symptoms of palpitation, depression, nausea, anorexia, diarrhea and vomiting were lower in observation group than in observation group, and the differences of depression, anorexia and vomiting were statistically significant(P<0.05);The BDNF index of the observation group (13.38 ±1.93)ng/L was higher than that of the control group (12.03 ±1.83) ng/L(P<0.05);CRP (3.53 ±0.97) mg/L and Cystatin C index (0.98 ±0.12) mg/L were lower than the control group(4.62 ±1.15)、(1.06 ±0.14) mg/L (P<0.05).After treatment, the glutathione peroxidate (GPX),superroxide dismutase(SOD),glutathione(GSH)and catalase(CAT) of oxidative stress in observation group were higher than those in control group,the difference was statistically significant (P<0.05).Conclusion Pramipexole in the treatment of Parkinson's disease is obvious, can effectively improve the serum CRP, Cystatin C and BDNF index, high security, it is worth promoting the application.

The effects of sodium salicylate on the expression of heat shock protein 27 (HSP27) during oxidative stress in tissue-cultured human lens epithelial cells were investigated. Cultured human lens epithelial cells (HLB-3) were divided into 3 groups: control group (group A), oxidation injury group (group B) and sodium salicylate group (group C). Apoptosis of human lens epithelial cells cultured in vitro was induced in the presence of 150 micromol/L H2O2. Cells viability and the expression of HSP27 were analyzed. Viability of the cells was measured by methyl thiazole tetrazolium (MTF) chromatometry. The expression of HSP27 in HLB-3 cells was detected by using immunohistochemistry and image analysis system. Sodium salicylate could induce the expression of HSP27, and the cells viability in group C was significantly higher than in group B (0.2667+/-0.01414 vs 0.2150+/-0.01080, P=0.012<0.05). The average gray value of HSP27 in group B was less than that in group C (P=0.000<0.05). The increased expression of HSP27 by sodium salicylate might play an important role in the protection of hydrogen peroxide-induced injury of human lens epithelial cells, suggesting that sodium salicylate could suppress, at least in part, the apoptosis of human lens epithelial cells.

Objective To evaluate the efficacy of uterosacral ligament amputation in combination with lesion electrofulguration under laparoscope in the treatment of endometriosis-related dysmenorrhea. Methods A total of 86 patients with endometriosis-related dysmenorrhea were treated by uterosacral ligament amputation combined with electrofulguration of lesions under laparoscope from March 2000 to August 2004 in this hospital. Among the 86 patients, enucleation of ovarian endometriotic cysts and electrofulguration of pelvic endometriotic lesions were also simultaneously conducted in 37 patients, and electrofulguration of all visible pelvic endometriotic lesions was also performed in the rest of 49 patients. The operation was accomplished with the aid of the placement of an infrared ureteral detector in 3 patients because of the thickening of the uterosacral ligament and local adhesion. Results The 86 patients were followed for 6~48 months (median, 23 months). Dysmenorrheal completely disappeared in 36 patients, partially subsided in 29, and remained unchanged in 21, the effective rate being 75.6% (65/86). Conclusions Uterosacral ligament amputation combined with electrofulguration of lesions under laparoscope is effective for the treatment of endometriosis-related dysmenorrhea.

Unusual dTDP-sugars are key intermediate in many pathogenic bacteria. In this study, negative-ion electrospray tandem mass spectrometry (ESI-MS-MS) with collision-induced dissociation (CID) was used to study the fragmentation characteristics of six unusual nucleotide diphosphate sugars. The results indicated the major fragment of the six unusual nucleoside sugars observed in the ESI-MS-MS spectra resulted from cleavage of diphosphate moiety and their characteristic fragment ions at m/z 401, 383, and 321, correspond to [TDP-H] together with fragment ions resulting from the loss of water and phosphate moiety, respectively. Furthermore, 4-position substituted change of unusual sugar rings affected the stability of two important characteristic fragment ions of [glycosyl-1"-PO3](-) and [glycosyl-1"-P2O6](-).

In recent years,nuclear medical imaging has become a routine examining technology.In general,angiographic medicine has to be injected into human body.Considering the short-coming of the manual injection,it seriously needs a injecting pump with the functions such as auto-control,push-pull injecting,synchronous injection of two injectors.

Objective To explore the clinical effect of integrated traditional Chinese and western medicine in the treatment of intractable hiccup caused by cerebrovascular disease.Methods A total of 74 patients with intractable hiccup caused by cerebrovascular disease were selected.The patients were randomly divided into observation group and control group according to random number table method.28 patients in the control group were given simple western medicine treatment.46 patients in the observation group were treated with integrated traditional Chinese and western medicine.The total effective rate and symptom improvement were observed after treatment.Results The total effective rate of observation group was 94.48%,that was 78.57% in the control group,the difference was statistically significant(x2=2.0,P<0.05).Conclusion Combined traditional Chinese and western medicine can significantly reduce intractable hiccup caused by cerebrovascular disease,improve total efficacy,improve the quality of life.

OBJECTIVE: To investigate the effects of low-level laser irradiation on acupuncture points combined with iontophoresis against brain damage after middle cerebral artery occlusion (MCAO) in rats. METHODS: Sixty-nine SD rats were randomly divided into five groups, including normal group, sham operation group, model group, electro-acupuncture group and low-level laser irradiation on acupuncture points combined with iontophoresis group (LLLI group). The cerebral ischemia-reperfusion (I/R) model was established by thread embolism of middle cerebral artery. The rats in the LLLI group, as well as the electro-acupuncture group were given treatment as soon as the occlusion finished (0 hour) and 12, 24 hours after the occlusion. We observed the changes of neurological deficit scores and the body weight of the rats at different time. The activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in the ratos brain tissue were tested. RESULTS: The neurological deficit score of the LLLI group was significantly lower than that of the model group. The body weight and the activity of SOD of the rats decreased slightly, and the content of MDA decreased significantly after the treatment. CONCLUSION: The low-level laser irradiation on acupuncture points combined with iontophoresis can prevent focal cerebral ischemia-reperfusion injury. One of its mechanisms may be increasing the activity of SOD and decreasing the damage of the oxidation products to the body.

Objective To investigate the biological characteristics of mouse erythrolenkemia cell FBL-3.Methods The morphological feature, growth characters, clone formation and immunochemistry of mouse erythroleukemia cell FBL-3 were examined by light microscope. The cell cycle distribution and expression of MHC were detected by flow cytometry. Drug sensitivity was measured by MTT assay. Tumorigenicity was evaluated after intravenous injection FBL-3 cells into C57BL/6 mice. Results FBL-3 cells were smaller,fusiform or polygon, adherence. Doubling time was 24.12 h. The clone formation rates were (35.23±1.44) %and (60.27±5.56) % at 14 th and 21 th day, respectively. The reactions for PAS and chloracetic acid were positive, while the POX, NAP and butanoic acid reactions were negative. The cell cycle distribution was as follow: G0/G1 phase (50.9±2.5) %, S phase (36.3±1.4) %, G2/M phase (13.8±0.8) %. The IC50 of FBL-3 cells to Ara-C, VDS, DDP, MMC and MTX were (0.49±0.04), (0.87±0. 09), (3.77±0.32), (1.66±0.16) μmol/L and (2.77±0.24) nmol/L, respectively. Chromosome number was at 34 to 41. MHC of FBL-3 cell was H-2b. Sexual gene Sry was positive. All C57BL/6 mice were morbidity with erythroblastic leukemia when FBL-3 cells had been intravenously inoculated. There was a linear relationship between the survival time and the number cell injected. The main targets for the leukemic FBL-3 cells were liver, spleen, kindey and lung. Conclusion FBL-3 cell has typical features of mouse leukemia cell, was easily cultured in vitro and tumorigenesised in C57BL/6 mice. FBL-3 cell could be as a satisfactory tool for the research of leukemia.

Objective To explore the relationship of the expression level of monocyte Chemotactic protein-1 and nuclear factor κB(NF κB)with atrial fibrosis of atrial fibrillation patients with rheumatic heart disease.Methods About 200 mg right atrial tissue were taken from 26 patients with rheumatic heart disease undergoing valve replacement surgery and divided into sinus rhythm (SR) group (n=12) and atrial fibrillation (AF) group (n=14).Masson staining and atrial myocardial fibrosis markers were used to determine the level of fibrosis.The mRNA levels of cytokines in atrial tissue were measured by reverse transcription polymerase chain reaction (RT-PCR)The protein level of MCP-1 and phosphorylation of NFκB in atrial tissue were detected by Western blotting.Results Compared with SR group,the AF group showed that collagen volume fraction (AF:0.42 ± 0.03;SR:0.13 ± 0.02),the mRNA levels of myocardial fibrosis markers such as transforming growth factor (TGF)-β1,type Ⅰ collagen and type Ⅲ collagen,and cytokines such as IL-1β and TNF-α,and the protein level of MCP-1 (AF:0.170±0.003;SR:0.040±0.005) and level of phosphorylation of NF-κB (AF:0.35 ± 0.02;SR:0.12 ± 0.03) were significantly increased (all P＜0.05).In addition,the expression levels of cytokines,the protein expression level of MCP1 and the phosphorylation level of NF-κB were positively correlated with collagen volume fraction of the right atrial myocardial tissue (all P＜0.05).Conclusions Activation of NF-κB may induce the expression of MCP-1 in the myocardial tissue of patients with rheumatic heart disease,and sequentially stimulate the secretion of cytokines and extracellular matrix deposition,and finally participate in the occurrence and persistence of atrial fibrillation.

Objective To investigate the clinical application value of stathmin, p16 and Ki-67 in the cervical intractable cases. Methods Immunohistochemical method was used to detect the expressions of stathmin, p16 and Ki-67 in surgical specimens of 288 cervical intractable cases, including 30 cases of cervical benign changes, 70 cases of cervical intraepithelial neoplasia (CIN)Ⅰ, 78 cases of CINⅡ, 85 cases of CINⅢand 25 cases of squamous cell carcinoma (SCC, as control group). The application value of stathmin, p16 and Ki-67 in the cervical cases were analyzed. Results The positive expression rates of Ki-67 of cervical benign changes and CINⅠwere 20.0 % (6/30) and 54.3 % (38/70) (χ2 = 3.29, P> 0.05). The expression rates of Ki-67 in CINⅡ, CINⅢ and SCC were all 100.0 %, and compared with the cervix benign changes, the differences were statistically significant (χ2= 112, P< 0.05). The expression rates of p16 in cervical benign changes and CINⅠwere 6.7 % (2/30) and 91.4 % (64/70), and there was significantly statistical difference (χ2=50.64, P<0.05). However, the expression rates of p16 in CINⅡ, CINⅢand SCC were all 100.0%, and compared with the cervix benign changes, the differences were statistically significant (χ2= 7.18, P< 0.01). The expression rates of stathmin in cervical benign changes, CINⅠ, CIN Ⅱ, CINⅢ and SCC were 3.3 %(1/30), 5.7 % (4/70), 23.1 % (18/78), 77.0 % (67/87) and 100.0 % (25/25), respectively, and there was no statistic difference in cervical benign changes, CINⅠand CINⅡ (χ2=0.68, P>0.05), but the expression rates in CINⅢ and SCC were higher than those in cervical benign change, CINⅠand CIN Ⅱ(P< 0.01). The positive expressions of stathmin, p16 and Ki-67 in each group of CIN were positively correlated (r= 0.412, P< 0.05). Conclusions Combined detection of p16 and Ki-67 can assist in the differential diagnosis of cervical intractable cases, and provide objective indicators for the classification and accurate diagnosis of CIN. Combined detection of p16 and stathmin may help to identify high-grade, low-grade CIN and cervix benign changes for the reduction of over-treatment.