Objective To study the in-vitro anti-tumor activities of physalin B (PhB) extracted from Physalis angulata L.. Methods Th e effect of PhB on the cell proliferation and the morphology changes of tumor cell strains HepG2 and SGC7901 was observed by methyl thiazolyl tetrazolium (MTT) assay and by phase contrast microscopy respectively, and the results were compared to positive control anti-cancer drug hydroxycamptothecine (HCTP) . Apoptosis of HepG2 and SGC7901 cells induced by PhB was determined by 4’ , 6-diamidino-2-phenylindole ( DAPI) staining method. Results MTT results showed that the growth of HepG2 and SGC7901 was significantly inhibited by PhB in dose-and time-dependent manner, and the inhibitory effect of high concentration of PhB was better than the positive control medicine HCTP. Phase contrast microscopy results showed that the cellular morphology was improved by PhB, which was accorded with the results showed by MTT assay. DAPI staining results showed that the apoptosis of HepG2 and SGC7901 was enhanced by PhB. Conclusion PhB extracted from Physalis pubescens L. exerts certain inhibitory effect on the proliferation of HepG2 and SGC7901 cells.

With the development of health care, the use of modern medical devices and the improvement of hospital manage-ment system, Intensive care unit( ICU) emerges.ICU is a treatment-nursing ward, which is a collection of modern medical care tech-nology.With ICU information constructive practice of Nanjing General Hospital of Nanjing Military Region as the background, this pa-per introduces the target base、functional requirements、business processes of the intensive care information system management mode of operation from the perspective of clinical application.Furthermore, this paper describes the realization of the function of the mode of operation with cases as evidence.

Objective The aim of this paper was to develop the Mobile Nutrition Order System based on the technology of hospital information system to adapt the nutrition and food security management model in the new situation, so that the unified informa-tization management of diet for patients could be implemented by the Department of Nutrition. Methods The Mobile Nutrition Order System, with the functions of information entry, nutrition recipe, order making and data transmission, could be constructed by the com-bination of technologies between informatization and digital order. Results The automation of nutrition order in hospital was a-chieved, and the nutrition recipe and nutritional therapy could be realized in hospital, the effect was very obvious. Conclusion Since the application of Mobile Nutrition Order System, the labor intensity of servers has been reduced, saving working hours effective-ly.Meanwhile, the management of nutritional therapy was improved and the quality of logistics services in hospital and nutritional ther-apy ascend to a new high.

Nowadays,the study of tumor stem cells has become the heatpoint in cancer research.Many experiments have successfully demonstrated the existence of tumor stem cells,which have been isolated from some solid tumors.As the research on the origin of tumor stem cell is developing,the knowledge of the occurrence and development of tumor has become more clear,which will influence the diagnosis and treatment for tumor significantly.Moreover it will bring benefit to the following-up after surgical operation and giving hopes to cancer sufferers.

[Objective] To analyse the constituents of Viscum ovalifolium DC. [Methods] The chemical constituents of Viscum ovalifolium DC were isolated by silica gel column chromatography, purified by crystallization and identified by spectroscopy method. [Results] Lupeol acetate, ?-amyrin and oleanolic acid were isolated and identified. [Conclusion] Lupeol acetate, ?-amyrin and oleanolic acid are isolated from Viscum ovalifolium DC for the first time.

Objective:To study the inhibitory effects of an adenovirus(Ad)-based short hairpin RNA(shRNA) expression system on expression of vascular endothelial growth factor receptor(VEGFR) and on growth of colon carcinoma cells in vitro and in vivo.Methods: RNA interference pAd-Easy/VEGFR vector was used to transfect 293 cells via Lipofectamine 2000.The adenoviral vector carrying VEGFR was used to transfect CW2 cells and the transfection efficiency was determined by fluorescent microscope and flow cytometry.The expression of VEGFR was examined by RT-PCR and Western blotting.The cell growth was observed with MTT method and the growth curve was plotted.Nude mouse was transplanted with CW2 cells to establish colon cancer tumor model and the growth of tumor was observed daily.Micro-vessel density(MVD) was detected by immunohistochemistry.Results: The recombinant pAd-Easy carrying shRNA against VEGFR was verified by sequencing.Flow cytometry showed that the transfection efficiency of CW2 cells was 99.7%.RT-PCR and Western blotting showed that the expression of VEGFR in pAd-Easy/VEGFR group was obviously decreased.The growth curve showed that the cell growth in the pAd-Easy/VEGFR group was obviously slowed down.We also found that the tumor growth in the nude mouse model was obviously inhibited and the MVD was also decreased.Conclusion: pAd-Easy/VEGFR-mediated VEGFR shRNA can effectively inhibit the expression of VEGFR in CW2 cells and suppress tumor growth in vivo.

Objective Abstract:Objective To explore the effects of arsernic trioxide(As2O3) on telomerase activity and cellular apoptosis of human colon cancer cell line CCL-187.Methods CCL-187 cells were divided into control group,1.0 ?mol?L-1As2O3 group and 2.5 ?mol?L-1As2O3 group,all groups were cultivated for 1 to 6 d.The inhitory rate of cell growth was detected by MTT assay.The morphologic changes of CCL-187 cells were observed under electron microscope.The expression of hTERT at mRNA level was analyzed by RT-PCR.Telomerase activity was determined by TRAP-PCR-ELISA.Results The differentiation of cells was found in 1.0 ?mol?L-1 As2O3 group,but apoptosis in 2.5 ?mol?L-1 As2O3 group,and at the same time,the telomerase activity was reduced and the hTERT-mRNA expression was downregulate in 1.0 ?mol?L-1 As2O3 group and 2.5 ?mol?L-1 As2O3 group in a time-dependent manner.There were significant differences compated with control group(P

Objective To investigate the changes and clinical significance of platelets aggregation rate of patients with coronary heart disease before and after percutaneous transluminal coronary stenting Methods There are 13 patients underwent coronary angiography and 20 patients underwent percutaneous transluminal coronary stenting Their blood samples were taken from the coronary sinus and coronary artery before and after interventional therapy Results The level of platelets aggregation rate in percutaneous transluminal coronary stenting group was higher after interventional therapy ( P

Objective To investigate advantages and disadvantages of endoscopic thyroidectomy.Methods A total of 61 consecutive patients with thyroid diseases from December 2003 to August 2004 in this hospital were freely divided into two groups: the Endoscopic Group(30 patients) and the Open Group(31 patients).The operating time,blood loss,hospital stay,postoperative pain assessment,jugular sensation 3 months after operation, swallowing function,and cosmetic outcomes between the two groups were compared.Results The operating time in the Open Group was significantly shorter than that in the Endoscopic Group(78?34 min vs 126?53 min;t=4.125,P=0.000).The intraoperative blood loss was less in the Endoscopic Group(20?13 ml) than in the Open Group(34?19 ml)(t=-3.313,P=0.002).The postoperative pain in the Endoscopic Group was slighter than that in the Open Group 24 and 48 hours after operation(t=3.255,P=0.002;t=-2.598,P=0.012).Three months after operation,jugular hypesthesia and discomfort at swallowing occurred in 1 and 2 patients in the Endoscopic Group,respectively,whereas jugular hypesthesia or paresthesia occurred in 11 patients(?~2=10.594,P=0.001) and discomfort at swallowing happened in 9 patients((?~2=5.773),P=0.016) in the Open Group.All patients in the Endoscopic Group were satisfied with the cosmetic results,but 19 patients in the Open Group complained of unsatisfactory cosmetic outcomes(?~2=52.141,P=0.000).Conclusions Endoscopic thyroidectomy gives little blood loss,mild postoperative pain,and good cosmetic results,being a feasible and safe procedure.

Proteins from dried roots of three medicinal Panax species (P. Quinquefolius, P. ginseng and P. nologinseng) were analyzed by SDS-PAGE and Western-blot. The protein fin- gerprints of Panax species were confirmed. From 28KD to 58KD and 55KD are in common. Very specific fingerprints of P. quinquefolius are found below 28KD. The fingerprints above 58KD seemed to be peculiar to P. ginsing. Hence the Western-blot approach can be used as a means to authenticate the concerned Panax species, especially for identification of P. quinquefolius.