Objective To explore the association between interleukin-10 (IL-10) gene polymorphism and susceptibility to Henoch-Schonlein purpura (HSP)in children,and to further explore the pathogenesis of HSP,provide some scientific evidence for the diagnosis,treatment and prognosis of HSP.Methods One hundred and twelve patients with HSP(HSP group),in which 39 cases were HSP nephritis (HSPN group) hospitalized in Zhengzhou Children's Hospital from December of 2012 to April of 2013 and 184 healthy children (healthy control group)were recruited in this study.The distribution of patients and the healthy control group in single nucleotide polymorphisms (SNPs) of IL-10 (rs1800872,rs3024490,rs3790622,rs1518111,rs1554286,rs3024498) was detected and compared by Seque-nom Massarray system analysis and meanwhile the haplotype analysis and linkage disequilibrium test were conducted.Simultaneously,the serum levels of IL-10 between HSP group and healthy control group were detected.Results The concentration of IL-10 in HSP group was higher than that of the healthy control group (P ＜ 0.01).No significant differences were found in gene distribution between HSP group and the healthy control group (P ＞ 0.05).For haplotypes analysis,ATCATA,AGCGCA were the most common haplotypes,followed by CTCATA,CGCGCA and ATTATA haplotypes.By the chi-square test,the levels of AGCGCA,ATCGCA,CTCATA haplotypes in HSP group were significantly higher than those in the healthy control group(P =8.41 e-012,0.003 536,1.30e-011).While the levels of ATCA-TA,CGCGCA haplotypes in HSP group were significantly lower than those in the healthy control group (P =0.003 519,4.94e-005).In the rs3790622,the allele frequency and the genotype frequency of the HSPN group were significantly higher compared with those of the healthy control group (all P =0.01).Conclusions The children with HSP are closely linked to the serum levels of IL-10.No significant associations were found between these variants and the susceptibility to HSP.For haplotypes analysis,the ATCATA,CGCGCA haplotypes may play a protective role in HSP children.In the rs3790622,the T allele and CT genotype may be the protective factor in HSPN.

Objective To observe blood pressure variability (BPV) in elderly patients with primary hypertension and carotid artery intima-media thickness (IMT),and analyze the correlation between BPV and atherosclerosis.Methods One hundred and nine patients with primary hypertension were divided into non-carotid atherosclerosis group (IMT ＜ 1.0 mm,49 cases) and carotid atherosclerosis group (IMT ≥ 1.0mm,60 cases).24 h ambulatory blood pressure monitoring (ABPM) was used to measure the 24-hour mean systolic blood pressure (24 h SBP),24-hour mean diastolic pressure (24 h DBP) ; SBP of daytime (dSBP)and nighttime (nSBP) ; DBP of daytime (dDBP) and nighttime (nDBP).And their standard deviation and coetficient of variation (CV) was calculated.Results dSBP,24 h SBPCV,24 h DBPCV,dSBPCV,nSBPCV in carotid atherosclerosis group was higher than that in non-carotid atherosclerosis group [(132.3 ± 12.1)mm Hg(1 mm Hg =0.133 kPa) vs.(122.7 ± 10.8) mm Hg,0.118±0.011 vs.0.107 ± 0.023,0.142 ± 0.058vs.0.116 ±0.028,0.129 ±0.039 vs.0.105 ±0.017,0.119 ±0.060 vs.0.109 ±0.037],and there was significant difference (P ＜ 0.01 or ＜ 0.05).There was no significant difference in 24 h SBP,24 h DBP,dDBP,nSBP,nDBP,dSBPCV,nSBPCV between two groups (P ＞0.05).Conclusions BPV of carotid atherosclerosis is higher than that of non-carotid atherosclerosis.BPV and carotid artery IMT has a certain relevance.

Giardia lamblia is an important intestinal protozoan which can cause diarrhea in humans. The detection of Giardia infection is performed through the detection methods of pathogen，immunoassay and molecular biology. Currently，the immunodiagnostic methods have good application and development prospect because of high sensitivity and specificity，simple and convenient，and time saving. In this article，we review the main progress and application of immunodiagnostic methods for Giardia infection.

ObjectiveTo investigate left ventricular (LV) radial dyssynchrony and LV rotational change of isolated complete left bundle branch block (CLBBB) and CLBBB with heart failure (HF).Methods Twenty-six isolated CLBBB patients,twenty-four CLBBB patients with HF and twenty healthy controls were studied.Two-dimensional speckle tracking imaging (2D-STI) was applied to short-axis views to calculate LV radial dyssynchrony and LV rotational and twist degree and time to peak rotational and twist degree.ResultsLV radial dyssynchrony and LV rotation and twist degree were intermediately damaged in isolated CLBBB patients and was significantly damaged in CLBBB patients with HF,compared with healthy controls.ConclusionsFor isolated CLBBB patients,even if LV ejection fraction was in the normal range,another parameters of cardiac mechanic had changed and 2DSTI could be used to evaluate these alterations.

Aim To study the effect of 2,3,4′,5-tetrahydroxystilbene-2-O-?-D glucoside(TSG) on vascular smooth muscle cells(VSMCs) proliferation induced by fetal bovine serum and explore the underlying mechanisms.Methods VSMCs were cultured from rat aorta using explant technique.VSMCs proliferation was analyzed by 5-bromo-2-deoxyuridine(Brdu) incorporation assay.Cell cycle phase distributions were determined by flow cytometry.The expression of proliferating cell nuclear antigen(PCNA) was evaluated by Western blot analysis.The level of intracellular reactive oxygen species(ROS) was estimated through fluorescence assay.The intracellular activities of superoxide dismutase(SOD),catalase(CAT),and glutathi one peroxidase(GSH-px) were measured using biochemical method.Results TSG in the experimental concentration 0.1～100 ?mol?L~-1 exhibited no apparent cytotoxocity.10 ?mol?L~-1 and 100 ?mol?L~-1 TSG significantly inhibited the proliferation of VSMCs induced by serum,cell cycle transition from G0/G1phase to S phase,PCNA expression in nucleus of VSMCs and level of intracellular ROS.100 ?mol?L~-1 TSG markedly increased the activities of SOD and GSH-px.Conclusions Certain concentration of TSG can prevent VSMCs proliferation effect mediated by serum;the mechanism about TSG effect may be associated with arresting G0/G1 to S progression,decreasing PCNA expression and improving the antioxidation of VSMCs.

Objective To explore the effect of preservation of inclined fractured permanent molars by transverse fixation with screw post,and provide a practical method for reserving the inclined fractured permanent molars.Methods Clinical data of 50 patients with inclined fractured permanent molars by transverse fixation with screw post in the Department of Stomatology of Tianjin First Central Hospital between January 2010 to December 2011 were reviewed retrospectively.After fixing the crown with steel-wire binding and root canal treatment,the molars were drilled a hole buccolingually and placed screw post.The flowable light-curing composite resin was used to seal the crack and bond screw post to prevent the relative movement.All treated molars were restored with full crown.All patients were followed up for 10-60 months.The therapeutic effects were observed.Results After 5 year-follow-up,41 cases were cured completely,6 cases were cured clinically,and 3 cases failed treatment.Conclusion The transverse fixation with screw post and bonding technique can preserve the inclined fractured molars to gain chewing function and avoid or reduce the extraction of affected teeth,which provides a better restoration effect.

AIM and METHOD: The relationship between the evolution of the reticulin fibres(RF) or the collagen fibres(CF) and the growing of hematopoietic cells in long-term bone marrow culture (LTBMC) from 15 paticnts with acute myeloid leukemia (AML) and form 6 normal subjects was observed by inverted microscope, Gomoris staining and Massons staining were used. RESULTS: (1)The amount of RF contents of 8 AMLs,with self- maintained(AMLsm) in the 1～8 weeks-old-culture was significant less than that of normal control and 7 AMLs,without self-maintained(AMLnsm) ( P

Objective To explore the relationship between plasma homocysteine levels and diabetic retinopathy (DR).MethodIn 40 DR patients and 63 non-DR patients,the indexes including plasma homocysteine concentrations,duration of diabetes,ages,blood pressure,preprandial blood glucose(PBG),plasma GHbA,e,plasma lipids,serum folie acid and semm vitamin B12 were analyzed.ResultsPlasma homocysteine level was significantly higher in DR patients (12.9μmol/L) than that in non-DR patients (7.8μ.mol/L) (P＜0.01),and the relationship remained significant correlation after adjusted for duration of diabetes,GHbA1c,ages,serum folic acid and serum vitamin B12(OR = 1.23,P＜0.05).ConclusionHyperhomocysteinimia is a risk factor and an independent predieator of DR.

Objective To observe the trend of temperature changes after ventriculoperitoneal shunt and its relation with postoperative infection.Methods A retrospective cohort study was conducted on 272 patients underwent ventriculoperitoneal shunt in our hospital from January 2015 to December 2018.Patients were divided into infected group and non-infected group according to whether intracranial infection occurred after surgery.According to the occurring time of postoperative intracranial infection,patients were divided into early infection group and delayed infection group.Temperature levels of all patients were monitored on the surgery day,and on the first,yd,5th and 7th d of surgery,and the temperature changes and fever rate of the two groups were compared.Results Among 272 patients,intracranial infection occurred in 25 (9.19％),including 21 with early infection and 4 with delayed infection,and un-infection in 247 (90.81％).As compared with those in the non-infected group,the fever rate and body temperature of the infected group were significantly higher on the 3rd,5th and 7th d after surgery (P＜0.05).There was no significant difference in fever rate and body temperature changes between the early infection group and the delayed infection group on the surgery day and on the first,3rd,5th and 7th d of surgery (P＞0.05).Conclusions The fever rate is higher and the body temperature decreases faster after ventriculoperitoneal shunt.The fever since the third d of surgery is closely related to postoperative intracranial infection,which has a certain predictive significance for postoperative infection,so we should consider anti-infection treatment in time.

ObjectiveTo explore the value of virtual touch tissue quantification (VTQ) in the evaluation of clinical pathological typing of advanced gastric cancer.MethodsFifty six patients who had been diagnosed as gastric cancer were examined using acoustic radiation force impulse.According to clinical pathological typing,all cases were divided into highly-moderately differentiated adenocarcinoma (14 cases) and non-highly-moderately differentiated adenocarcinoma (42 cases).A comparison with clinical pathologic results was made after surgery.The correlation of VTQ results and clinical pathological typing of gastric cancer was analyzed.ResultsThe VTQ value of highly-moderately differentiated adenocarcinoma was lower than that of non-highly-moderately differentiated adenocarcinoma [(1.49 ± 0.44) m/s vs (2.12 ± 0.45) m/s],with statistical significance( t =-4.53,P ＜0.05).According to the maximum area under the ROC curve,the cutoff value of VTQ was 1.795 m/s,the sensitivity and specificity were 78％ and 86％,respectively,the Youden's index was 0.64,and with high reproducibility(Kappa =0.81).ConclusionsVTQ could initial estimate the clinical pathological typing of advanced gastric cancer before operation.