OBJECTIVE:To optimize the best formula of the total alkaloid tablets of peganum harmala.METHODS:3 levels from each of the amount of four factors-excipient calcium carbonate,tween-80,starch and emollient(magnesium stearate:french chalk) were subjected to the orthogonal design with the parameter of dissolution(Td) of the harmine in tablet as an index.RESULTS:The amount of tween-80 and calcium carbonate(P

By analyzing data from a questionnaire survey on bilingual teaching, we evalueted the results of bilingual teaching for seven-year medical students and the main problems of bilingual teaching and solutions accordingly. An uneven English level on the part of teachers and students and imperfect materials etc. affect the overall results of bilingual teaching. Therefore persistent efforts need to be made in enhancing the teachers' English level, improving teaching methods and compiling proper textbooks so as to genuinely improve the bilingual teaching program.

Objective To investigate the effect of griping exercise on wrist palmar swelling after percutaneous coronary intervention. Methods A total of 90 patients of the First Affiliated Hospital of Zhengzhou University with planned percutaneous coronary intervention via radial artery were enrolled and divided into the control group, griping exercise group 1 and griping exercise group 2 with 30 cases in every group according to the sequence of admission. Preoperative education and postoperative care were performed with a conventional approach in the control group. Griping exercise in group 1: Fisting exercise with electronic grips in 15 times per min, lasted 1 min, 2 hours per day for 3 days. Griping exercise in group 2: Fisting exercise with electronic grips in 25 times per min, lasted 2 min, 2 hours per day for 3 days. The degree of swelling and pain in patients′wrist palmar 6 hours after operation (sheath tube removal) were observed. Results The pain score in wrist and palm in griping exercise group 2 was 0.83±0.39, and 4.20±1.98 in the control group, the difference was significant (t=3.912, P < 0.05), and also lower than that of the griping exercise group 1 (1.92±0.78, t=3.087, P < 0.05). The swelling value of finger circumference in griping exercise group 2 was (0.13±0.05) cm, and in the control group was (0.39±0.13) cm, the difference was significant (t=3.013, P < 0.05), also lower than (0.23±0.11) cm in the griping exercise group 1 (t=2.612, P < 0.05). Conclusions Fisting exercise with electronic grips in 25 times per min, lasted 2 min, 2 hours per day for 3 days preoperation could reduce the swelling and pain in patients′wrist and palm on the operation side.

BACKGROUND:The elderly hip fracture patients with chronic obstructive pulmonary disease (COPD) have a higher postoperative mortality than those only with hip fractures. In recent years, it has become an issue of concerns. Because the mechanism is unknown, however, there are no effective clinical interventions for these patients. OBJECTIVE:To observe the effect of bone marrow stromal stem cel s (BMSCs) on the level of pulmonary inflammation in aged rats with chronic obstructive pulmonary disease (COPD) after hip fractures. METHODS:Thirty male Sprague-Dawley rats, 12 months old, were exposed to smoking for 4 months and randomized into three groups, smoking, smoking+hip fracture+normal saline, smoking+hip fracture+BMSCs groups. Animal models of hip fracture were made in the latter two groups. Twenty-four hours after hip fracture, the lower lobe and peripheral blood samples were taken from al rats in the three groups, to evaluate the pathological changes of lung tissue and detect levels of inflammatory factors in the lung tissue and peripheral blood. RESULTS AND CONCLUSION:After closed hip fracture, aged COPD rats exhibited inflammatory cel infiltration, mucus secretion, airway stenosis or occlusion;the levels of interleukin-6, tumor necrosis factor-αand interleukin-10 in the lung tissue and peripheral blood were increased. After intravenous injection of BMSCs, the pathological changes of the lung tissue were reduced, and the levels of pro-inflammatory factors, tumor necrosis factor-αand interleukin-6, decreased, but the level of anti-inflammatory factor interleukin-10 further increased, which were significantly different from those in the normal saline group (P<0.05). These findings indicate that BMSCs can relieve acute lung injury in aged COPD rats with hip fractures.

Odontogenic keratocyst(OKC)is a common jaw cyst with a high recurrence rate.OKC combined with basal cell carcinoma as well as skeletal and other developmental abnormalities is thought to be associated with Gorlin syndrome.Moreover,OKC needs to be differentiated from orthokeratinized odontogenic cyst and other jaw cysts.Because of the different prognosis,differential diagnosis of several cysts can contribute to clinical management.We collected 519 cases,comprising a total of 2157 hematoxylin and eosin-stained images,to develop digital pathology-based artificial intelligence(AI)models for the diagnosis and prognosis of OKC.The Inception_v3 neural network was utilized to train and test models developed from patch-level images.Finally,whole slide image-level AI models were developed by integrating deep learning-generated pathology features with several machine learning algorithms.The AI models showed great performance in the diagnosis(AUC=0.935,95%CI:0.898-0.973)and prognosis(AUC=0.840,95%CI:0.751-0.930)of OKC.The advantages of multiple slides model for integrating of histopathological information are demonstrated through a comparison with the single slide model.Furthermore,the study investigates the correlation between AI features generated by deep learning and pathological findings,highlighting the interpretative potential of AI models in the pathology.Here,we have developed the robust diagnostic and prognostic models for OKC.The AI model that is based on digital pathology shows promise potential for applications in odontogenic diseases of the jaw.

Objective To discuss the clinical values of transabdominal ultrasonic diagnosis of nontraumatic hiatal gastric hernia of esophagus in middle-aged and elderly people.Methods Thirty-two cases were examined with fasting and filing methods in half-sitting,standing,supine and supine right anterior oblique positions.The findings were contrasted with X-ray barium meal examination,endoscopy and operation.Results Twenty-nine cases were of sliding hiatal gastric hernia of esophagus,3 of impacted hernia.The ultrasonic diagnosis coincidence rate was 100%.The signs and symtoms of acoustic images were widening of esophageal hiatus diaphragmatic muscle(≥1.37 cm),echoes of gastric mucosa were found in superior phrenic hernail sac and inside hernial sac.The display rates were 100%,100%,15.6%respectively.Conclusions The transabdominal ultrasonic diagnosis of nontraumatic hiatal gastric hernia of esophagus in middle-aged and elderly people is brief and accurate.

Objective To explore the microneurosurgical technique and prevention of postoper-ative complications for the fourth ventricle tumors in adults. Methods We retrospectively analyzed the clinical data of 68 patients with the fourth ventricle tumors between August 2005 and August 2007 in Xiangya Hospital after microsurgical operation. Tumors were excised by inferior vermis cere-bellar approach or cerebellomedullary fissure approach. The extent of tumor removal should take into consideration the possible injury of brain stem respiratory center, especially tumors adherent to the brain stem. Cerebral aqueduct obstructions were removed in all patients, suspending dura on the neck muscles during closing skull to eliminate scalp hydrops. Results There were 58 total tumor excisions and 10 subtotal tumor excisions. No patient died and no suboccipital hydrops took place before dis-charge in this study. Postoperative symptomatic hydrocephalus was found in 10 patients, but it was cured by ventricle-abdomen shunt. Hemorrhage in tumor lumen happened in 4 patients, who received second microsurgery. Drugs were given to 8 patients with intracranial pneumatocele, 10 with intracra-nial infection, and 18 with upper gastrointestinal hemorrhage. Five patients out of the 16 tracheoto-mies recovered well by mechanical ventilation. Conclusion Protecting the life center of brain stem and dredging the aqueduct outlet completely were the key to surgical success. Therapeutic effect could be improved by adept microneurosurgical techniques after operation. The prognosis of patients may be improved by preventing complications actively and combined therapy after the operation.

Objective To explore the effect of doctor-nurse integration health education on early off-bed ambulation of patients with hepatocellular carcinoma after surgery.Methods A total of 52 primary liver cancer patients from June 2015 to May 2016 were selected as control group by historical controlled sampling,and were given by routine method to guide off-bed ambulation,and 55 primary liver cancer patients from June 2016 to May 2017 were selected as intervention group,given doctor-nurse integration health education.The time to first bed-off ambulation and duration of postoperative ambulation,postoperative anxiety and satisfaction between the two groups were compared.Results The time to first bed-off ambulation and duration of postoperative ambulation,anxiety score and patients' satisfaction of the two groups showed statistically significant differences (P ＜ 0.05).Conclusion The doctor-nurse integration health education can effectively promote rapid recovery of patients after liver cancer surgery.

Objective To explore the effect of doctor-nurse integration health education on early off-bed ambulation of patients with hepatocellular carcinoma after surgery.Methods A total of 52 primary liver cancer patients from June 2015 to May 2016 were selected as control group by historical controlled sampling,and were given by routine method to guide off-bed ambulation,and 55 primary liver cancer patients from June 2016 to May 2017 were selected as intervention group,given doctor-nurse integration health education.The time to first bed-off ambulation and duration of postoperative ambulation,postoperative anxiety and satisfaction between the two groups were compared.Results The time to first bed-off ambulation and duration of postoperative ambulation,anxiety score and patients' satisfaction of the two groups showed statistically significant differences (P ＜ 0.05).Conclusion The doctor-nurse integration health education can effectively promote rapid recovery of patients after liver cancer surgery.

Background and purpose: Cancer stem cells (CSCs) isolated from human glioma are cancer-initiating cells and sources of tumor recurrence in brain tumors. The poor outcome of glioma is because cancer stem cells can not be eradicated. This article was aimed to explore the resistance of CSCs to chemotherapeutic agents and expression of drug-resistance enzymes in glioma cancer stem cells. Methods: Cancer stem cells from U251 were isolated by using magnetic sorting. The proliferation inhibitory effects of Vumon-26 (Vm-26), bischloronitrosourea (BCNU) and diamminedichloroplatinum (DDP) on U251-CSC and U251 were examined by drug sensitivity testing in vitro (MTT assay) and the apoptosis rates were observed by flow cytometry. Western blot was performed to examine the expression of three drug-resistance enzymes including LRP, MGMT and Topo Ⅱα. Results: Chemotherapeutic agents had a more obvious inhibitory effect on U251 than U251-CSC, as well as higher apoptosis rates. LRP, MGMT and Topo Ⅱα expression were significantly higher in U251-CSC as compared to U251, Conclusion: Glioma stem cells showed strong capability of tumor's resistance to chemotherapeutic agents including Vm-26, BCNU and DDP. This resistance is probably contributed by the CD133 positive cell with higher expression of on LRP, MGMT and Topo Ⅱα.