Objective To investigate the clinical value of 64 slice spiral CT low dose chest scan in the diagnosis of pulmonary tuberculosis.Methods From January 2015 to January 2016,45 patients with suspected pulmonary tuberculosis were studied.All patients underwent routine chest CT scan and low dose chest CT scan.To compare the difference of radiation dose related parameters between normal dose chest CT scanning and low dose chest CT scan.To observe the display of the characteristics of lung disease by routine dose chest CT scanning and low dose chest CT scan.Results In 45 patients,there were a total of 30 patients were diagnosed with tuberculosis.The diagnostic sensitivity,specificity and diagnostic coincidence rate of conventional CT scan and spiral CT low-dose scan were 93.3% and 86.7% respectively.In low dose CT scanning radiation dose related parameters,the effective dose [(1.36±0.22)mSv vs.(5.24±0.68) mSv],CT dose index[(31.8±3.7) vs.(51.2±6.3)] and dose length product[(35.4±5.4)mGycm vs.(137.9±26.1) mGycm]were significantly lower than those of the conventional dose CT scan,the differences were statistically significant (all P<0.05).Conventional dose and low dose CT scan could show the lung lesions of pulmonary tuberculosis.Comparison between the two groups,low dose CT scanning on the burr sign and grinding glass shadow display rate was low.Compared with the conventional dose CT scanning,the difference was statistically significant (P<0.05).Conclusion In the diagnosis of pulmonary tuberculosis,the radiation dose of 64 slice spiral CT is significantly decreased,be able to clear most of the lung lesions.However,the effect is poor in the display of the grinding glass and the thin and short burr.

Objective To study relationship between the level of serum ICAM,ALCAM,VCAM and PECAM-1 in elderly patients with type 2 diabetes mellitus combined with acute cerebral infarction and its meaning.Methods From July 2014 to December 2015,60 patients with type 2 diabetes mellitus complicated with acute cerebral infarction were chosen as the research object.They were divided into three groups according to severity of atherosclerosis:no plaque in 11 cases,22 cases of stable plaques and unstable plaque 27 cases.40 cases with acute cerebral infarction patients and 40 cases of patients with type 2 diabetes were selected as a disease control;and other 40 healthy subjects were selected as healthy controls.ELISA were used to detect ICAM,ALCAM,VCAM and PECAM 1 level.Neural function evaluation was made by the U.S national institutes of health stroke scale (NIHSS).Results The ICAM,ALCAM,VCAM,PECAM 1 level in diabetic cerebral infarction group were higher than the other three groups,the difference was statistically significant (P＜0.05),while those of cerebral infarction group and diabetes group were higher than the control group,the difference was statistically significant (P＜0.05),and those of cerebral infarction group and diabetes group has no statistical significance (P＞0.05).NIHSS score of diabetic cerebral infarction patients was (7.39 ± 1.72),which was higher than that of patients with cerebral infarction group (5.33 ± 1.49),the difference was statistically significant (t=4.376,P =0.019,P＜0.05).The serum ICAM,ALCAM,VCAM,PECAM 1 level of diabetic cerebral infarction patients was a positively correlated with NIHSS score (r=0.559,P=0.007;r=0.619,P=0.000;r=0.421,P=0.018;r=0.451,P=0.007).With diabetic cerebral infarction is aggravating,the severity of carotid plaques in patients with serum ICAM,ALCAM,VCAM,PECAM-1 level is on the rise (P＜0.05).Conclusion ICAM,ALCAM,VCAM,PECAM-1 levels in peripheral blood serum of patients with type 2 diabetes mellitus complicated with acute cerebral infarction have an abnormal increase,and ICAM,ALCAM,VCAM,PECAM-1 level is closely related to the neurologic deficits and the severity of carotid artery plaque of type 2 diabetes mellitus complicated with acute cerebral infarction patients.

Cardiovascular and cerebrovascular diseases is the most fatal diseases in the world.The prevention and treatment of cardiovascular and cerebrovascular diseases are both basic and clinical focus.Aspirin has been used as a prevention medicine in cardiovascular and cerebrovascular diseases for over a century, which is the longest one in history.Aspirin use is estimated at 100 billion tablets annually as an analgesic, antipyretic and antiplatelet drugs.However, there are still many new findings about aspirin, such as aspirin resistance and aspirin hydrolase.This paper reviews the current research advances and future directions of aspirin in cardiovascular and cerebravascwlar diseases, mainly focuses on aspirin resistance and personalized medication.

Objective To analyze the clinical determination and mechanism of poststroke dementia.Methods 186 consecutively hospitalized patients who at admission after stroke,completed clinical valuation of neural-functional defect and intelligence assessments,and head CT scan.Their intelligene performances were assessed again after three months .Results Frequency of poststroke dementia was 32 5% .Poststroke dementia was related to age,level of education,residential area,hypertension ,hyperlipidemia,the location and severity of stroke,history of coronary heart disease and diabetes.But it unrelated to sex,smoking,alcohol drinking and type of stroke.Conclusions Dementia is frequently occurred after stroke,and multiple risk factors contribute to its occurrence

ObjectiveTo retrospectively analyze the relationship between clinicopathological,biological characteristics and the outcome of renal cell carcinoma(RCC) and evaluate the risk factors related to metastasis in young patients.MethodsThe data of 83 RCC patients younger than 40-year-old, treated from January 1986 to December 2007 in Tianjin Cancer Hospital,were analyzed retrospectively.The complete follow-up data of the 83 cases were collected.The operative methods included partial and radical surgery.Clinical staging were consistent with the 2004 UICC TNM classification criterion.The histological sections were reviewed.Various biological factors including VEGFR-1, VEGFR-2, VEGFR-3, PDGFR-α, PDGFR-β, c-kit and PCNA were tested by immunohistochemistry staining.The adjuvant radiation therapy, chemotherapy and immunotherapy after operation were carried out on the basis of pathological and biological features.The 83 cases were divided into two groups according to metastasis and non-metastasis status within 5 years.The prognosis related factors including clinical factors, pathological and biological factors were evaluated.Chi-square test was used for the analysis of 5-years metastasis status; and multivariate analysis was carried out using Cox proportional hazards models to assess the independent effect of the metastasis factors (the test standard α = 0.05).ResultsThe 5-year follow-up data showed that the metastasis rate in the 83 cases was 16.87％ (14/83).The Chi-square test results indicated that the patients with clinical stage Ⅲ and grade 3 had the highest metastasis rate (57.14％ and 78.57％respectively,x2 =38.042, x2 = 9.820; Ps ＜ 0.01) .The VEGFR-3 and PCNA positive expression rates were 92.86％ and 85.71％ respectively in metastasis group.The metastatic risk of early stage patients was 3.444 times as much as the advanced stage patients.ConclusionThe young patients with clinical advanced renal cell carcinoma had worse outcome.The stage,grade, VEGFR-3 and PCNA expression were the risk factors correlated with the metastasis risk for young RCC patients.TNM stage was an independent predictive risk factor of metastasis in young patients.

Objective To investigate the release and intracellular localization of high mobility group box chromosomal protein 1(HMGBl)in the peripheral blood monocytes of rheumatoid arthritis(RA) patients and the inhibitive effect of thaiidomide.Methods 19 RA patients and 20 healthy controls were included in the study.Monocytes were separated from peripheral blood with Ficoll density gradient centrifugation.Monocytes were treated with 100 ng/ml tumor necrosis factor α(TNFa)or 100 ng/ml TNFα plus 40 μg/ml thalidomide and grown in an incubator at 37℃ with 5%CO,for 24 hours.The cuIture supernatants of the monocytes were collected.HMGB1 level in the culture medium was detected with Western blot.In addition,the intraceUular localization of HMGB1 in the fflonocytes was investigated with immunocytochemical analysis. Results Without stimulation. the release of HMGBl protein was significantly increased in the culture supernatants of peripheral blood monocytes from RA patients as compared with that from healthy controls(P<0.05).TNFα(100 ng/ml)did not further increase the release of HMGBl in the monocytes from the patients with RA.Thalidomide(40 μg/ml)could inhibit the release of HMGB1 in the monocytes from RA patients stimulated with TNFα(P<0.05).In the monocytes from RA patients,HMGBl was mainly localized in the nucleus.Treatment with TNFOL(100 ng/ml)for 24 hour resulted in a cytoplasmic translocation of HMGB1,which was inhibited significantly by thalidomide. Conclusion TNFα induces the release and cytoplasmic translocation of HMGBI in the peTipheral blood monocytes of RA patients and thalidomide inhibits the release and translocation of HMGB1.

Objective To establish an assessment scale for hospital stroke patients with Delphi method,by using it to help the nurses evaluate patients' existing and potential problems,and to provide foundations for specific assessment on different patient groups.Methods The first-level indicators and second-level indicators were developed on the basis of the Omaha system.210 nursing records of hospital stroke patients were then combined with to form concrete items.Totally 22 nursing specialists were interviewed with two rounds in order to further collect the opinions and suggestions about the assessment scale.Results 4 first-level indicators,35 second-level indicators and a number of items were included in the assessment scale.Conclusions The assessment scale for hospital stroke patients established by Delphi method comprehensively shows the environmental,physical,mental and behavioral condition in hospital,and it could be used as a tool to assess the hospital stroke patients.

This article discusses how to improve teaching quality of health statistics from four main aspects: paying attention to training statistical thinking,paying attention to understanding basic concepts and methods,paying attention to modernistic teaching instrumentality,and paying attention to intercommunion between teachers and students.Practice indicates it facilitates impoving teaching quality.

Objective To provide a simple, convenient, and safe anesthesia method for the establishment of a M1 segment of middle cerebral artery occlusion model in rhesus monkey or other large laboratory animals.Method Twenty male rhesus monkeys weighing 7-11 kg (ages 7-9 years) from Academy of Military Medical Sciences were used in this study.Sumianxin injection combined with 0.1 mg/kg ketamine was given before endotracheal intubation (ID:4.5-5.5#).The animals were then transported to an interventional operation room, where the intravenous access was established and a urinary catheter was inserted into the urinary bladder.Mechanical ventilation was used during the surgery, propofol was continuously injected in a speed of 2-4 mg/kg/h, and Sumianxin-ketamine could be given if necessary to maintain adequate anesthesia depth.The dose was adjusted according to vital signs of the rhesus such as body movements, physiological parameters, and demand of surgery.Brain MRI examination was performed before and after thrombolysis.Anesthetic injection was suspended and the animals were allowed to have a spontaneous breathing every time before the MRI text.Heart rates, temperature, non-invasive blood pressure, and SpO2 were monitored during the whole surgery.Blood samples were taken from the radial artery for blood gas analysis after anesthesia induction and during operation.Results All the 20 animals underwent the operation successfully, no animal had restlessness, respiratory depression, arrhythmia and other serious complications.At the end of the study, animals awake soon.Fifteen of them survived longer than 24 hours, only 5 died from serious cerebral hemorrhage and larger cerebral infarction.Conclusions General endotracheal anesthesia is safe for rhesus monkeys during such interventional operation and MRI examination.

Objective To investigate the expression of XIAP, Smac, HtrA2 and XAF1 in the hippocampus following SE in rats and to explore the pathophysiological mechanisms of expression of XIAP and its negative regulators after SE. Methods The lithium-pilocapine model of status epilepticus was established in SD rat. XIAP, Smac, HtrA2, XAF1 and activated caspase-3 protein were examined using immunohistochemistry. Western blot was used to detect the protein levels of XIAP, Smac, HtrA2 and activated easpase-3. Results XIAP immunoreactivity diffusely distributed within the neuron after SE. Compared with the control group, the expression of CA3 XIAP protein in the SE group was increased gradually since 2 hours (0.5503±0.0172 vs 0.1507±0.0165, t=115.87, P<0.01), peaking at 8 hours (0.6221±0.0238 vs 0.1507±0.0165, t=136.69, P<0.01). The expression of CA3 Smac, HtrA2, XAF1 and activated caspase-3 protein were increased generally following SE. Western blot analysis showed a significant increase in Stoat, HtrA2, activated caspase-3 protein levels from 2 to 72 hours following SE, but no significant differences were seen in XIAP protein levels between the control group and the SE group. Conclusions The XIAP, Smac, HtrA2 and XAF1 are involved in the regulation of neuronal apoptosis and implicated in pathophysiological mechanisms of neuronal damage after SE.