Objective To evaluate the short-term effect of open and laparoscopic cholecystectomy surgery on body.Methods Forty patients with cholecystectomy were divided into open surgery group and laparoscopic surgery group by random digits table method with 20 cases each.The clinical index was compared between two groups.The immunoglobulin G(IgG) and procalcitonin (PCT) before surgery and 4 d after surgery were determined.Results The operative blood loss,anal exhaust time,length of stay was (135.80 ±26.74) ml,(1.6 ±0.9) h,(6.7 ±3.2) d in open surgery group,which was better than that in laparoscopic surgery group [(63.41 ± 15.06) ml,(0.4 ± 0.3) h,(2.8 ± 1.9) d],and there was significant difference (P ＜ 0.05).The incidence of heat was 15％(3/20) in open surgery group,which was higher than that in laparoscopic surgery group (0),and there was significant difference (P ＜0.05).There was no significant difference in the IgG and PCT between two groups before surgery (P ＞ 0.05).The IgG and PCT at 4 d after surgery was (13.93 ± 1.98) g/L,(2 153.2 ± 1 452.1) mg/L in open surgery group,which was higher than that in laparoscopic surgery group[(9.23 ± 2.87) g/L,(768.0 ± 237.0) mg/L],and there was significant difference (P ＜ 0.05).Condmion Laparoscopic cholecystectomy has smaller short-term effects on body,while the body of the humoral and cellular immune function in mild,conducive to the body's recovery.

Objective:To evaluate the single dose and dosage frequency of analgesic drugs in 9 Chinese cancer hospitals in 2012. Methods:Using a retrospective method, the utilization of analgesic drugs in 9 cancer hospitals in the year of 2012 was analyzed statis-tically. Results:Most of single dosage of 15 analgesic drugs was consistent with the package inserts except for flunarizine and pethidine oral preparations with single overdose up to 50%. Most of dosage frequency of 21 analgesic drugs was consistent with the package in-serts except for oxycodone/acetaminophen, dihydrocodeine/paracetamol and dezocine with inconsistent dosage frequency ratio up to 30%. Conclusion:Most of single dose and dosage frequency of analgesic drugs is consistent with the package inserts. There is some unreasonable usage that should be paid attention to. Clinicians should be more cautious in analgesic drug use to make correct and ra-tional decision on dose and frequency, meanwhile, drug use in patients should be monitored to ensure the medication safety.

Patients with long-standing history of ulcerative colitis have an increased risk of developing colitis-associated colorectal cancer.The risk factors include longer duration of colitis,greater anatomic extent of colitis,degree of inflammation of the bowel,family history of colorectal callcer,the presence of primary sclerosing cholangitis.etc.The two kinds of molecular pathogenesis that contribute to colon carcinogenesis are chromosomal instability and microsatellite instability.In order to reduce cancer morbidity,colonescopic surveillance and chemoprevention should be carried out in patients with ulcerative colitis especially in those high risk patients.New endoscopic techniques increase the sensitivity and shed a new light on tumor surveillance.

Objective To investigate the correlation between mental status of patients with stroke and their quality of life. Method One hundred and three stroke patients were measured by HRSD,HMAM and SS-QOL to explore the correlation between mental status and quality of life.Results Among the 103 patients,68 developed depression, taking up 66.02%,with a total score of 31.62±12.58;52 anxiety,taking up 50.49%,with a total score of 22.17±5.38.The level of depression and anxiety were negatively correlated with the life quality(P<0.01).Conclusions The occurence rate of depression and anxiety in stroke patients is higher. The higher the scores on depression and anxiety,the worse the quality of life.

Humeral condylar fracture is one of the common clinical fractures,the treatment is difficult for it often accompanied with their joint surface injury,Learn from past experience combined with our department for humeral condylar fracture treatment,this article was designed to review the different treatment of humerus fractures,analyze the advantages and disadvantages of each treatment and indications for detail,with an emphasis on the application of artificial elbow Joint replacement in the humeral fractures.With a view to enhance the understanding to the treatment of Humeral condylar fracture.

Long non-coding RNAs (lncRNAs) are non-coding RNAs that have transcript lengths exceeding 200 bp and do not have the capacity for protein coding because of having no open reading frame. In the human genome, lncRNAs play important regulato-ry roles in the process of epigenetic, transcription and post-transcription, so they have become the focus of research followed by mi-croRNAs. The abnormal expression of lncRNAs in cancer usually represents different functions, such as the function of oncogenes or tumor suppressor genes, which promote or inhibit tumor growth. Lung cancer is a common malignant tumor with a five-year survival rate of 17%. Previous literature shows that MALAT1, H19, lincRNA p21, UCA1, and BC200 are closely related to the development of lung cancer. They could promote cancer growth, invasion and metastasis, apoptosis and induce drug resistance, and so on. This review aims to provide assistance in the diagnosis, treatment, and prognosis of lung cancer by clarifying the functions and mechanisms of ln-cRNAs.

Objective To investigate the clinical value of simplified Chinese version NU-DESC in assessment of ICU delirium in domestic ICU,and provide easy ICU delirium assessment tool for clinical nurses.Methods We used simplified Chinese version NU-DESC to assess patients in domestic ICU from July to September 2011,and compared with the gold standard DSM-IV diagnosis results.Their validity,reliability and effectiveness were evaluated to analyze the application value of NU-DESC in ICU.Results The area under the ROC curve of simplified Chinese version of Nu-DESC was 0.951,the sensitivity was 82％,the specificity was 90％,the false negative rate was 18％,the false positive rate was 10％,Youden's index was 0.72,the positive likelihood ratio was 7.99,the negative likelihood ratio was 0.20,the agreement rate was 87％,Kappa value was 0.71,the positive predictive value was 77％,the negative predictive value was 92％.Conclusions The simplified Chinese version NU-DESC can easily be understood,used and accepted by nurses.They can be used as an assessment tool for nurses to judge ICU delirium for ICU patients.

Objective To explore the effect of risk management on the perioperative nursing of elderly patients undergoing cardiovascular surgery. Method Sixty-two elderly patients undergoing cardiovascular surgeries were managed by management, which included establishing risk management team, identity and evatuating risk factors, applying risk manadement. Result There was no nursing risk events among them. Conclusion For elderly patients with cardiovascular surgery, risk management can enhance nurses′ability of discerning, assessing and managing the nursing risks so that it is effective in avoiding possible risks, reducing nursing adverse events during perioperative period, improving nursing quality and ensuring the safety of surgery.

[ ABSTRACT] AIM:To compare the expression of SIRT2 in ovarian surface epithelial ( OSE) cell line and serous ovarian carcinoma ( SOC) cell lines, and to investigate the effects of SIRT2 on the cell proliferation, migration and inva-sion.METHODS:The expression levels of SIRT2 in the OSE cell line and the SOC cell lines were determined by Western blot.The SIRT2 siRNAs and overexpression construct were designed and verified.Transient transfection of SIRT2 siRNAs or overexpression construct was performed, and the effect of SIRT2 on the cell proliferation, migration and invasion was e-valuated.RESULTS:SIRT2 levels in the 5 strains of SOC cell lines were significantly lower than that in the OSE cell line.SIRT2 knockdown in HOSEpiC cells significantly enhanced the ability of cell colony formation and accelerated the cell growth rate.On the contrary, overexpression of SIRT2 in HO8910 cells dramatically repressed the number of cell colonies and cell activity.SIRT2 significantly changed the ability of ovarian cell migration.Knockdown of SIRT2 facilitated the cell invasion.CONCLUSION:The expression of SIRT2 in the SOC cells is significantly down-regulated.In the OSE cells, SIRT2 acts as a tumor suppressor and mediates the inhibition of cell proliferation, migration and invasion.

Objective:To observe the therapeutic efficacy of acupuncture plus heat-sensitive moxibustion in treating chronic pelvic inflammatory disease (CPID), for selecting an effective acupuncture-moxibustion method in treating chronic pelvic inflammation. Methods:Eighty CPID subjects were enrolled and divided into two groups by randomized single-blind method, 40 in each group. The observation group was intervened by acupuncture plus heat-sensitive moxibustion, while the control group was by acupuncture plus box-moxibustion, once every other day, 10 sessions as a treatment course, but the period time was skipped. The therapeutic efficacy was observed after 3 treatment courses. Results:The recovery rate was 45.0% and the total effective rate was 95.0% in the observation group, versus 20.0% and 80.0% in the control roup. There were significant differences in comparing the recovery rate and the total effective rate between the two groups (P<0.05). Conclusion:Acupuncture plus heat-sensitive moxibustion can produce a higher therapeutic efficacy in treating CPID than acupuncture plus box-moxibustion, and this is an easy-to-operate and safe method without adverse effect.