Ten cases of type I diabetes mellitus were treated with intracerebral pancreas islet transplantation. In 7 cases insulin has been totally stopped for 2 to 13 months, in other 2 cases the dosages of insulin had a decrease of 37% and 64% than that before transplantation respectively, and good control was achieved in those 9 patients. Only one case had not any improvement. The results showed that it is an effective therapy for type I diabetes mellitus and indicated that the pancreas islet was survived in patient's cerebrum and secreted insulin. No evidence of immunological rejection was found. Long-term follow-up is necessary to find how long the allogenic graft can survive.

Objective To investigate the role of islet B cell dysfunction in the pathogenesis of type 2 diabetes mellitus. Methods FBG and fasting plasma insulin of 49 healthy subjects and 125 first-degree relatives of type 2 diabetes mellitus were determined. HOMA model was used to calculate HOMAIR as an index of insulin resistance and HOMAB as an index of B cell function of pancreatic islet.The insulin sensitivity index(ISI) and early-stage secretion index of islet ?cell(△I_ 30 /△G_ 30 )were calculated. Results The HOMAB and △I_ 30 /△G_ 30 of the group of normal first-degree relatives were higher (P

Objective To investigate the effect on prevention and treatment autoimmune diabetes mellitus in NOD mice by oral administration porcine insulin.Methods One hundred and twenty female NOD mice were divided randomly into insulin group(n=60) and control group(n=60).Beginning from 6 week old, NOD mice were fed porcine insulin 500ul or phosphate buffered saline(PBS) 500ul seperately till 40 week old. At 6 ?12 ?20 ?25 ?30 week old,serum insulin of healthy mice was measured and pancreas were used for histology.Results Oral administration of insulin can marked decreased incidence of diabetes ( P

Clinical epidemiological studies have shown that vitamin D deficiency is associated with infectious diseases, which is confirmed by recent studies implying the role of vitamin D as a key regulator of host defense against infections. Bioactive vitamin D triggers antimicrobial pathways against pathogens in mucous membrane and cells of the human innate immune system. In addition, vitamin D also attenuates excessive inflammation and acquired immunity, and thus limits collateral tissue damage. The observed effects of vitamin D on infectious diseases, particularly airway infections, should be addressed in the management of viral infections. Findings from cross-sectional studies should be confirmed by prospective research. In this review, we summarize the current knowledge regarding the effect of vitamin D on innate and acquired host defense against infections.

Objective　To observe the effect of high glucose on retinal capillary pericytes in vitro and research the pathogenesis of diabetic retinopathy (DR). Methods　Retinas dissected from eyes of fresh killed cattle were separated and retinal capillary pericytes were cultured. The effect of high glucose on pericytes was evaluated by measurement of lactate dehydrogenase(LDH),3H-thymidine incorporation and liquid scintillation techniques.Morphological changes were observed under microscope and electric microscope.Results　There was no significant difference in LDH activity in the supernatants between high glucose(10、20、40mmol*L-1) and normal glucose(5mmol*L-1)cultured for 48h or 96h. High glucose had an effective inhibition on pericytes' proliferation cultured for 48h,96h and 144h, and the inhibition rate increased when the glucose concentration rised and time passed by. The inhibition rate of pericytes was 78.09% after cultured in high glucose (40mmol*L-1) for 144h. Conclusion　High glucose has an effect inhibition on pericytes' proliferation, and the inhibition rate increase when the glucose concentration rises and time passes by.

Objective To investigate the association between a 894 G→T mutation at exon 7 of eNOS gene and diabetic nephropathy in type 2 diabetes mellitus of northern chinese Methods A case control study for 228 chinese subjects (including 143 type 2 diabetes mellitus with or without nephropathy and 85 normal control)was performed The number of the 894 G→T mutation alleles were determined by polymerase chain reaction and restriction fragment length polymorphism(PCR RFLP) method combined by DNA sequencing Results The frequency of the T allele and TG genotype at exon 7 were significantly higher in DN+ group than in DN group and control subjects(P

ObjectiveTo observe the effect of dehydroepiandrostrone (DHEA) on retinal capillary pericytes’ proliferation under high glucose and research the prevention and treatment of diabetic retinopathy (DR).MethodsRetinas were dissected from eyes of fresh killed cattle and retinal capillary pericytes were separated and cultured.The effect of DHEA (10、50、100、500、1 000nmol/L) on pericytes’ proliferetion was evaluated by 3H-thymidine incorporation and li-quid scintillation techniques.ResultsThere was significant difference between DHEA (50、100、500、1 000nmol/L) and control culturing for 48h.The increasing rate was 42.67% in DHEA of 500nmol/L.ConclusionDHEA could promote pericytes’ proliferation under high glucose.This provided reliable evidence for selecting effective drugs for prevention and treatment of DR clinically.

Objective To investigate the effects of captopril on structure and function of sciatic nerve in diabetic rats. Methods Streptozotocin induced diabetic male Sprague Dawley rats were treated with 0.1% captopril in drinking water for 12 weeks, then advanced glycation end products (AGEs), cAMP content, and the nerve conduction velocity of sciatic nerve in diabetic rats were measured, morphometric quantitative analysis and ultrastructure of sural nerve were also performed. Results Captopril reduced accumulation of AGEs, restored synthesis of cAMP in sciatic nerve, improved nerve conduction velocity partialy, and ameliorated morphology abnormalities in myelin nerve fibre. Conclusion These findings suggest that captopril may exert a therapeutic effects on diabetic peripheral neuropathy.

To investigate the insulin resistance and insulin secretion of the first degree relatives of T2DM with normal glucose tolerance(NGT). The levels of FIns and HOMA-IR in the groups of the parental (n=35) and the maternal (n=58) were higher than that in the group of the paternal (n=42), bwt the IAI and △I_ 30 /△G_ 30 were lower than that in the group of paternal. This suggests the first-degree relatives of type 2 diabetes have insulin resistance and impaired first phase insulin secretion in NGT.

0.05),and the distribution of genotypes was in Hardy-Weinberg equilibrium.Data analysis showed Met326Ile variant did not impact on FIns,FPG,HOMA index,BMI and gene expression of PI3-K,though the expression of PI3-K gene was lower in type 2 diabetes than in control group.Conclusion The Met326Ile variant of the p85? regulatory subunit of PI3-K is likely to be functionally normal in type 2 diabetes and health people.