Background and purpose:Nausea and vomiting were the most common side effects of chemotherapy and they are difficult to control especially in terms of delayed nausea and vomiting induced by CDDP. Acupuncture treatment is popularly the traditional medical method used in China,being economic and easy to carry out.This research is to study the role of acupuncture treatment in the prevention of NP regimen induced delayed nausea and vomiting. Methods:26 patients with NSCLC were enrolled into a randomized double blind crossover trial. The patients received either hydrochloride ondostrone+Dexm+acupuncture treatment(the first cycle of chemotherapy) →hydrochloride ondostrone+Dexm (the second cycle of chemotherapy)(defined as AB group) or hydrochloride ondostrone+Dexm (the first cycle of chemotherapy)→hydrochloride ondostrone+Dexm+acupuncture treatment (the second cycle of chemotherapy)( defined as BA group).Results:Twenty six patients were evaluated. The complete controls of nausea for the AB group with acupuncture treatment were 96.15%, 96.15%, 76.92%, 84.62%, 92.31% and 100.00% for day 1-6, respectively, for the BA group without acupuncture treatment were 100.00%, 76.92%, 57.69%, 34.62%, 65.38% and 76.92%, respectively. There were statistically difference in terms of the control of nausea for day 3-5(P

T-2 toxin is a secondary fungal metabolite that belongs to the trichothecene mycotoxin family,it is the most toxic in class A trichothecene mycotoxin.T-2 toxin can induce apoptosis in cells bearing high proliferating activity.The mycotoxin readily passes the placenta and is distributed to embryo tissues,which results in fetal brain damage,bone malformation,thymic atrophy and even death.This article reviewed the effects of T-2 toxin on immune system,blood system and cell toxicities in pregnant mice and generation mice.

Objective To investigate the correlation between the incidence of thyroid carcinoma and TSH, and to provide evidence for clinical application.MethodsSelect 450 cases of patients with thyroid from August 2014 to August 2016 in our hospital as the object of study in this group, the analysis of gender and age of patients, lymph nodes, tumor, laboratory examination results, to explore the relationship between TSH levels and thyroid cancer.ResultsThyroid patients with TSH was significantly higher than that in benign tumor group (P<0.05);the level of TSH and patients with lesion diameter, tumor stage, lymph node metastasis and capsule invasion was significantly correlated (P<0.05), but the level of TSH and age, gender, tumor number, vascular invasion and no significant correlation;TSH the level of thyroid cancer patients were significantly increased (P<0.05).ConclusionThe level of TSH in serum is closely related to the occurrence and development of thyroid cancer, and is closely related to lymph node metastasis and tumor stage, and the higher TSH level can increase the risk of thyroid cancer.

T-2 toxin is a secondary fungal metabolite that belongs to the trichothecene mycotoxin family.T-2 toxin can lead to the structural and functional changes of cartilage cells and cartilage cell degeneration and necrosis.With strong cytotoxicity,T-2 toxin can cause definite damage to cartilage cells.In this paper,we reviewed recent studies on the effects of T-2 toxin on chondrocyte apoptosis,ultra structural changes and extracellular matrix in vitro.

Objective:To establish a content determination method for the decoction pieces of ginseng radix et Rhizoma and investi-gate the quality of the slices by measuring the content of ginsenoside in commercially available crude materials and the decoction pieces. Methods:An HPLC method was used to determine the content of ginsenoside Rb1, Re and Rg1 in the crude materials and decoction pieces of ginseng radix et Rhizoma. Results:Significant difference in ginsenoside content between the medicinal materials and decoction pieces was found, and saponin content in the slices was decreased. Conclusion:The quality of the slices of ginseng radix et Rhizoma is changed significantly, and it is urgent to develop a agreed standard for the quality control of ginseng radix et Rhizoma during the process-ing.

Objective To assess the left ventricular(LV) regional myocardial systolic function after bone marrow mesenchymal stem cells (BMSCs) injection in rabbits with acute myocardial infarction(AMI) by 2-dimensional ultrasound speckle-tracking imaging.Methods Twenty-four healthy rabbits were randomly divided into three groups:group of sham-operated,group of masculine control (AMI was induced by ligation of left anterior descending coronary artery),and group of cell infusion(after two weeks of AMI,bone marrow mesenchymal stem cells were injected to the region of AMI).Four weeks after cell deliver two-dimensional strain images were acquired from LV short-axis view(at the levels of mitral annulus,muscle papillary and apex),radial strain rate (SrR),circumference strain rate (SrC),rotation rate (RotR) and rotation (Rot) of three levels in short-axis views were measured by speckle-tracking imaging.The conventional echocardiography indices included LV diameter of end diastole(LVEDd),LV ejection fraction(LVEF),LV fractional shortening(LVFS).The catheter indices included LV systolic pressure(LVSP),LV end diastolic pressure (LVEDP)and maximum rate of rise and descend of LV pressure(LVdp/dtmax).ResultsCompared with sham-operated group,rabbits had significantly larger LVEDd,lower LVEF and LVFS,lower LVSP and LVdp/dtmax,higher LVEDP in control group,SrR,SrC,RotR and Rot of LV anterior regional myocardial function and global myocardial function of three levels in short-axis views were lower.Compared with control group,the rabbits had significantly smaller LVEDd,larger LVEF and LVFS,larger LVSP and LVdp/dtmax,lower LVEDP,SrR,SrC,RotR and Rot of LV anterior regional myocardial function and global myocardial function of three levels in short-axis views were larger in group of cell infusion.There was no significant between group of cell infusion and group of sham-operated.Conclusions Speckle tracking imaging can evaluate the regional myocardial systolic function of the area of BMSCs transplantation accurately.

BACKGROUND: The mRNA expression of α1 chain of type I collagen (COL- Iα1)in rat osteosarcoma (ROS17/2.8) induced by bone morphogenetic protein-2(BMP-2)was reduced under simulated weightlessness. The protein kinase MEK1 in the signal pathway of mitogen-activated protein kinase (MAPK) plays an important role in the expression of COL- I αl mRNA regulated by BMP-2. But there was no report on the kinase activity of MEK1 under simulated weightlessness.OBJECTIVE: To investigate the effects of simulated weightlessness on the activity of MEK1 induced by BMP-2 in ROS17/2.8 cells.DESIGN: A non-randomly control study was conducted.SETTING: Department of Aerospace Biodynamics, Fourth Military Medical University of Chinese PLAMATERIALS: Rat osteosarcoma osteoblast-like cell METHODS: This experiment was conducted at the Laboratory of Aerospace cells and Molecular Biology Laboratory , Institute of Space Medico-Engineering between August 2002 and January 2003. ROS17/2.8 cells were cultured in 1 G control and rotating clinostat simulated weightlessness for 24 hours, 48 hours and 72 hours. The cells were divided into 7 groups as follows: Group 1 was blank control group in which ROS17/2.8 cells were cultured in 1 G condition without BMP2; Group 2, cells were cultured in 1 G for 24 hours;Group 3,in weightlessness for 24 hours; Group 4, in 1 G for 48 hours; Group 5, in weightlessness for 48 hours;Group 6,in 1 G for 72 hours;Group 7,in weightlessness for 72 hours. Cells in Group 2 to Group 7 were all cultured with BMP-2. BMP-2 (500 mg/L) was added into the medium 1 hou before the culture ended. Then the total protein of cells was extracted and the kinase activity of MEK1 was detected by means of Western Blotting.MAIN OUTCOME MEASURES: The content of total ERK1/2 and phosphated-ERK1/2 (p-ERK1/2) protein in the cells RESULTS: ①Total ERK1/2 expression induced by BMP-2 in ROS17/2.8 cells under simulated weightlessness: The total ERK1/2 expression induced by BMP-2 in cells in all the groups showed no significant differences ② Phosphated-ERK1/2 expression induced by BMP-2 in ROS17/2.8 .cells under simulated weightlessness: There was little protein of p-ERK1/2 in ROS17/2.8 cells in Group 1 cultured without BMP-2 in 1 G for 24 hours. The content of p-ERK1/2 in ROS17/2.8 cells in Group 2 cultured with BMP-2 in 1 G for 24 hours was much more than that in Group 1 (P ＜ 0.01). The level of p-ERK1/2 was much lower in simulated weightlessness groups than that in 1 G control groups at the same time point. In other words, the content of p-ERK1/2 in Groups 3, 5 and 7 was respectively lower than that in Groups 2, 4 and 6 (P ＜ 0.01).The expression of p-ERK1/2 showed-a tendency of gradually decreasing in Groups 3, 5 and 7 with the prolongation of time of simulated weightlessness(P ＜ 0.01 ).CONCLUSION: The kinase activity of MEK1 in MAPK signal pathway induced by BMP-2 is reduced under simulated weightlessness.

Objective: To investigate in vitro the biological characteristics of AdCD80-infected human lung cancer cells on the basis of generation of replication-deficient hB7-1(CD80) recombinant adenovirus. Methods: Human CD80 gene was transduced into lung cancer cells mediated by recombinant adenovirus and then the expression of the gene was detected by PCR and agarose gel electrophoresis. The biological characteristics of the above cells were analysed with electron microscope, FACS and etc. Results: The titers of rAd reached to 10 10 PFU/ml and more than 90% Anip973 lung cancer cells could be infected by 30 MOI rAd. The growth curve and cloning efficiency of rAdCD80-infected Anip973 cells showed no significant difference compared with that of the control cells. The cell proliferation cycle of rAdCD80-infected 973 cells showed no change through FACS test. Having been infected by rAdCD80, the surface structure and ultrastructure of 973 cells had a little change. Conclusion: These results will lay foundation for tumor vaccines.

ObjectiveTo observe the clinical efficacy of joint needling plus heat-tonifying needling in treating knee osteoarthritis (KOA).MethodEighty KOApatients were randomized into atreatmentgroup and a control group. Thetreatmentgroup was intervened by joint needling plus heat-tonifying needling. The control group was intervened by ordinary acupuncture. The Visual Analogue Scale (VAS) from the short-form McGill Pain Questionnaire (SF-MPQ) was adopted to evaluate the pain intensity.Result The total effective rate was 92.5% in thetreatmentgroup, versus 87.5% inthe control group, and there was a significant difference in comparing the VAS score between thetreatmentgroup and the control group after intervention (P<0.05), indicating that the treatmentgroup had advantages in the improvement of the motor function of knee joint.ConclusionIn treating KOA, joint needling plus heat-tonifying needling has advantages in improving the motor function compared with ordinary acupuncture.

Objective To comparative analysis the rabbit VX2 tumors angiogenesis in fatty liver and normal liver,and investigate the correlation of contrast-enhanced ultrasound (CEUS) parameters and their angiogenesis.Methods Rabbit models of fatty liver and normal liver with implanted VX2 tumors were established.Two groups of hepatic backgrounds and VX2 tumors were analyzed by QontraXt quantitative software of CEUS.The expression level of tumor microvascular density (MVD) and vascular endothelial growth factor (VEGF) were detected with immunohistochemical techniques and real-time fluorescence quantitative PCR (FQ-PCR).Results ①No significant difference was found in MVD,VEGF protein and gene expression between VX2 tumors in normal liver and fatty liver.The expression of VEGF gene in fatty liver parenchyma were lower than normal liver parenchyma (P ＜0.05).②There was positive correlation between MVD and peak intensity (r =0.494,P ＜0.05; r =0.591,P ＜0.01) both in fatty liver and normal liver VX2 tumors.The expression of VEGF protein were not correlated with TIC parameters (all P ＞ 0.05).③The MVD had positive correlation with expression of VEGF protein in fatty liver and normal liver VX2 tumors (r =0.508,P ＜0.05; r =0.570,P ＜0.01).Conclusions The expression of MVD and VEGF had no significant difference between fatty liver and normal liver VX2 tumors.The peak intensity of VX2 tumors CEUS had positive correlation with MVD both in fatty liver and normal liver,which can indirectly reflect MVD expression level and help to evaluate tumor angiogenesis.