Objective To estabilish a HPLC method for the determination of Geniposide in Jigucao Capsule. Methods The column was Lichrospher-C18(5 ?m,4.6 mm?250 mm). The determination was carried out with acetonitrile-0.1% phosphoric acid(10∶90) as the mobile phase,a flow rate of 1.0 mL/min and detected at 238 nm,and temperature of the column was 35 ℃. Results The calibration curve showed good linearity in the range of 0.14～1.68 ?g,Y =1 552.15X-6.42,r=0.999 9. The mean recovery was 97.53%(RSD=1.24%,n =6). Conclusion The method is accurate,reliable,and can be used for quality control of the preparation.

Objective To investigate the association between renin-like activity (RA) and angiotensin Ⅱ (AⅡ) and the ovarian hyperstimulation syndrome (OHSS). Methods Blood samples were taken from 42 patients undergoing in vitro fertilization (IVF). According to the ovarian stimulating response, the patients were divided into 4 groups: group Ⅰ, low responders, 7 cases; group Ⅱ, moderate responders, 8 cases; group Ⅲa high responders without using albumin, 7 cases, group Ⅲb, high responders with albumin, 10 cases; group Ⅳ, severe and moderate OHSS, 10 cases. 5 patients for intrauterin insemination with natural cycle as control. Follicular fluid (FF) was collected at oocyte retrieval from 28 IVF patients (including 7 OHSS patients). Fluid from ascites and hydrothorax was obtained from 3 OHSS patients, and peritoneal fluid obtained from 5 infertility patients by laparoscopy and ascitic fluid obtained from 6 ovarian carcinoma patients served as control. RA and AⅡ levels were measured by radioimmunoassay. Results plasma RA and AⅡ levels at mid-luteal phase from OHSS patients [(19.9?19.0)?g?L -1 ?h -1 and (397.0?378.2) ng/L] were significantly higher than those from the other patients ( P

Astrocytes play many important roles in the nervous system.In recent years,researchers are paying attention to the roles of astrocytes in the process of stroke.This article mainly reviews the biological functions of astrocytes and the gene expression changes of astrocytes after ischemic stroke,thereby providing new potential therapeutic targets for the treatment of ischemic stroke.

Osteogenic differentiation of mesenchymal stem cells (MSCs) is an important link of bone metabolism and bone repair, the mechanism of which is usually complicated. Recent studies have found that BMP, FGF, Wnt, Hedgehog, NELL1, IGF, Notch and othersignaling molecules are involved in the regulation of osteogenic differentiation of MSCs. These signaling molecules themselves have been shown to have a strong ability to promote osteogenic differentiation. They can activate the corresponding receptors by specific ligands, thus initiating the specific signal transduction pathways in the cell, and finally promote the transcription and translation of the genes related to bone formation. Furthermore, all the signaling pathways form a complex network which regulates and influences the osteogenic differentiation ofMSCs. Further researches on the mechanism of the interaction of these signaling pathways is of great significance in promoting the development of bone tissue engineering and the treatment of bone metabolic diseases.

ObjectiveTo summarize and investigate the occurrence,clinical features and treatment of cesarean scar pregnancy.Methods33 cases of cesarean scar pregnancy were retrospectively analyzed.Results10 cases of vaginal massive hemorrhage after uterine curettage,9 cases of subtotal hysterectomy,1 case was cured by MTX treatment,13 cases were cured by conservative treatment of uterine curettage after chemotherapy treatment,10 cases of successful uterus-saving by excision of lesion and uterine repair.ConclusionTransvaginal sonography was an important method in diagnosis of cesarean scar pregnancy and,the key to success was to select the appropriate individual treatment according to classification by transvaginal sonography.

OBJECTIVE:To establish a method for the identification of Chongqing local medicinal material Heracleum hemsley-anum. METHODS:The original plant morphology,morphological characteristics,microscopic characteristics (transverse section and powder),were identified. RESULTS:H. hemsleyanum had cone shape root,brown,many-branched with special fragrance;the microscopic characteristics of taproot a transverse showed no differences with lateral root transverse section,primary xylem was di-arch,secondary phloem was more developed than secondary xylem,secondary phloem was rich in the oil chamber. Vascular tissues contained a large number of parenchyma cells. In TLC chromatrogram,the test sample showed the same color fluorescent spots with-the substance reference of osthole and columbianadin in the corresponding locations. CONCLUSIONS:The established method is simple with high accuracy,and can be used as the basis for identification of H. hemsleyanum.

Objective To discuss the feasibility of the lung transplantation as an effective treatment for end-stage pulmonary diseases domestically and to compare the major issues involving the practice of lung transplantation domestically and overseas.Methods After the foundation of group of lung transplantation in May 2002, lung transplantation models of porcine were set up. From September 2002 to April 2005, in the 18 cases undergoing lung transplantation, there were subjected to single-lung transplantion (SLT, 83.3%) and 3 bilateral sequential single lung transplants without CPB (DSSLT, 16.7%). Indications for SLT (n=15) included emphysema (n=9), pulmonary fibrosis (n=3), pneumosilicosis (n=1), lymphangioleiomyomatosis (n=1) and ventricular septal defect (VSD) induced Eisenmenger's syndrome (n=1); for DSSLT (n=3) bronchiectasis (n=1) and pulmonary emphysema (n=2). Among the 15 SLTs, there were 9 cases of left SLT and 6 right SLT. Among them, 2 cases shared one same donor's lung block, one case received contralateral lung transplantation―a left donor lung implanted in the recipient’s right thorax, and one case simultaneous right SLT and VSD repair. Results In hospital mortality (HM) was 3/18 ( 16.7%). Among SLTs, early death was due to severe rejection on the 30th postoperative day in one patient and acute rejection on the 15th postoperative day in other patients, and another patient died due to pulmonary vein embolism on the 36th day. There were 3 and 2 patients with the survival time longer than 1 and 2 years respectively. The median overall survival was 10 (2 to 32) months. Conclusions Our LT program shows similar results to those reported by the International Society for Heart and Lung Transplantation for developed countries. The key of successful operation depends on the establishment of group of lung transplantation and cooperation of multi-department. Ischemic-reperfusion injury, acute-rejection and infection are the major reasons of deaths shortly after the operations.

AIM: To find a more efficient and accurate method for medical image enhancement to resolve the problem of low local contrast, which often appears in magnetic resonance (MR) images.METHODS: Based on the investigation into the previous methods, a new algorithm was presented. The main features were as follows: Non-dual was used in the morphological operations and addition instead of multiplication was used in the contrast stretching operations. To avoid some gray-level bias, the method of normalized gray-level under condition was proposed.RESULTS: The new algorithm was tested by real MR images and simulated experiments. Compared with the previous method, the new method is more accurate, faster and less sensitive to noise.CONCLUSION: Non-dual morphological operations can achieve local contrast enhancement in a more accurate way, and the gray level bias can be eliminated by normalization.

Objective To investigate the factor relateted to the prognosis in HFRS patients with ARF oliguresis stage and the applied chance of hemodiaglysis in HFRS patients. Methods 46 cases of HFRS patients with ARF, with hemodiaglysis applied hospitalized in the department of infectious disease of Luohe centre hospital during 1996 to 2004 were retrospectively investigated and the factor related to the prognosis were analyzed,divided into three groups:successful group,delayed group, died group, and 14 sorts of clinical factors and common lab test items compared. Results Between live groups, the following factors are statistics value,such as the age, platelet,serum albution before HD,frequency of HD and the days of oliguresis stage, the difference of the other factors such as chance of HD,laboratory indicators and complication occuring rate of are no notable. The age of died group is in one′s prime, died in 3 hospitalized days, given HD only 1~2 times,so,chance of HD and days of oliguresis stage were without comparing value,but,the rate of acute complication is 100 percent,the difference is notable.Conclusions The influence of HD time is no notable,and, to prevent acute complication before HD is the key factor relateted to the prognosis.

OBJECTIVE:To provide reference for curriculum setting for postgraduates majoring in Science of Chinese materia medica. METHODS:The curriculum setting for postgraduates majoring in Science of Chinese materia medica was comprehensively analyzed from the aspects of the regional differences and unique differences of curriculum setting and correlation of curriculum set-ting combined with professional interest of students,actual needs of the professional field and relevant circumstances of industry de-velopment. RESULTS & CONCLUSIONS:The curriculum setting exists regional differences and unique differences. It should show positive correlation with professional interest of students,actual needs of the professional field and relevant circumstances of industry development. It needs further exploration and improvement in the construction of curriculum to make it more scientific and systematic,and the curriculum contents should be able to match the training mechanism and pattern for the cultivation of postgradu-ates .