OBJECTIVE:To understand the main diseases research area and tendency of the literatures published on top-five global biomedicine journals,and provide reference for topics and designs of domestic medical research. METHODS:Studies pub-lished on NEJM,JAMA,BMJ,Lancet and PLoS Medicine from 2004 to 2013 were searched via Web of Science;types of disease were classified by using International Classification of Diseases(version 10)(ICD-10);research types were analyzed according to the rules in clinical epidemiology. RESULTS:Most of studies(25.8%)were published on BMJ;USA(39.1%),UK(25.7%) and Canada(5.1%)were top-three countries for the first authors;circulation system disease,infectious and parasitic diseases,can-cer,endocrine,nutritional and metabolic disease and respiratory system disease were top-five in disease categories list,accounting for 61.7%;ischemic heart disease,HIV-related disease,diabetes,other heart disease and influenza and pneumonia were the main top-five specific diseases;and randomized controlled trials were main study type. CONCLUSIONS:The proportion of the first au-thor for the Chinese literature in the host country is very low. Top-five specific diseases are placed on circulation system disease,in-fectious and parasitic diseases,endocrine,nutritional and metabolic disease and respiratory system disease,with the main study types of randomized controlled trial,cohort study and systematic review.