1.The Study of Tumor Suppressor Gene PTEN Expression and Its Correlation with the P53 Expression in Cholangiocarcinoma
Yanguang WEN ; Huihuan TANG ; Weijia SUN
Journal of Chinese Physician 2001;0(09):-
Objective To investigate the expressions of phosphatase and tensin homolog deleted on chromosome 10(PTEN) and P53 protein in cholangiocarcinoma and evaluate their possible relationship and clinical significance. Methods The expressions of PTEN and P53 in 36 cases of cholangiocarcinoma, eight cases of normal biliary duct and 30 cases of para-neoplastic tissue were detected by S-P immunohistochemistry method. Results The positive rate of PTEN protein expression was 63.89%(23/36) in cholangiocarcinoma, which was significantly lower than that in normal biliary duct (100%,8/8) and that in para-neoplastic tissue(100%,30/30), and which had no relationship with tumor locus, but was significantly correlated with the degree of differentiation and metastasis. The positive rate of P53 protein expression was 44.44%(16/36) in cholangiocarcinoma, which was significantly higher than that in normal biliary duct( 0%, 0/8) and that in para-neoplastic tissue (0%, 0/30), and it had no relationship with tumor locus and metastasis,but was significantly correlated with the degree of differentiation. The PTEN protein expression had correlation with the expression of P53. Conclusion The expression of PTEN protein was significantly lower in cholangiocarcinoma and significantly correlated with the degree of differentiation and metastasis other than the locus of tumor. The expression of P53 protein was associated with the degree of differentiation, but not with the tumor locus and metastasis. Moreover, The PTEN protein expression had correlation with the P53 expression.
2.The inhibitory expression of VEGF in antisense blocking of PTTG gene on human cholangiocarcinoma cell line QBC939
Ling CHEN ; Huihuan TANG ; Chao FEN ; Yanguang WEN ; Zhiyuan JIAN
Chinese Journal of General Surgery 2001;0(10):-
Objective To investigate the effect of expression of pituitary tumor transforming gene(PTTG) on the expression of inhibitory of vascular endothelial growth factor(VEGF).Methods The constructed(recomibinant) vector,pcDNA3.1-PTTGas that contained full-length antisense PTTG,was transfected into the cholangiocarcinoma cell line QBC939 in different quantity.After 48h incubation,the variation of expressions of PTTG and VEGF mRNAs and proteins were observed by RT-PCP and Western-blot method.Results(After) successful transfecting the recomibinant vector into QBC939,the mRNAs and proteins of PTTG and VEGF were all inhibited.Linear correlation analysis showed positive correlation between descendent extents of PTTG and VEGF(P
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