Objective Better drying methods of Forsythia suspense leaves was studied to provide some basis for the development and utilization of Forsythia suspense leaves. Methods A RP-HPLC method was used on XTerraTMRP8 (3.9 mm?150 mm, 5 ?m) column with acetonitrile-0.1% acetic acid solution (12 : 88) as mobile phase for forsythoside. The detection wavelength of forsythoside was at 328 nm and detection temperature was 30 ℃. Results The contents of forsythoside was higher when dry, microwave drying, absorbent paper drying, steaming 2～10 min, cooking 2～10 min was used to deal with Forsythia suspense leaves. The contents of forsythoside was lower when dried, drying, infrared drying, vacuum drying was used to deal with Forsythia suspense leaves. Conclusion Steaming 2～10 min, cooking 2～ 10 min is more appropriate for drying of Forsythia suspense leaves, when factors of the contents of forsythoside, the cost and ease of operation were considerd.

Objective Chromatography fingerprint of Radix Codonopsis of different age from Shanxi province was studied to provide the reference for the science appraisal of intrinsic quality of Radix codonopsis. Method A RP-HPLC method was applied on GraceSmart-RP18 (250 mm?4.6 mm, 5 ?m) column with acetonitrile-0.1% acetic acid gradient elution as mobile phase, and the flow-rate was 0.6 mL/min. The detection wavelength was at 268 nm and the column temperature was at 30 ℃. Result Chromatography fingerprint of Radix Codonopsis can demonstrate obviously the difference and the similarity of Radix Codonopsis of different age. The RSD were lower than 3% in the precision, stability and the repetitiveness trial, and the codonopsis pilosula alkyne glucoside can be used as the reference to identify the active composition of Radix Codonopsis. Conclusion The method was simple, reproducible and reliable. The integrity of fingerprint can be used to supply the reference for the quality control of Radix Codonopsis from Shanxi provice.

Object To study IR absorption spectra of the extracts from chicken bile possessed excellent anti inflammatory and anticancer activity so as to develop this valuable resource.Methods Bile samples from chickens and various animals were dried in the dark and subjected to IR analysis respectively. Results By comparison, significant difference was found between IR spectra of chicken bile and other animals' bile, but the IR spectra of various chicken bile showed very good consistency. Conclusion The IR spectra of chicken bile is independent of raising places, species, feeding fashion, sampling season and age of sampled chicken. It was suggested that the bile of various chicken contained same chemical composition and this result was confirmed by UV and TLC analysis.

Objective:To investigate the differences of psychological condition of erectile dysfunction (ED) patients in different age groups.Methods:The emotion, personality and interpersonal relationship outpatients with ED were evaluated by the method of Symptom Checklist-90 (SCL-90), State Trait Anxiety Inventory (STAI) and Eysenck Personality Questionnaire (EPQ). A retrospective analysis was conducted and a total of 401 ED patients (aged 20-60) were divided into 4 groups [20-29(n=158), 30-39(n=182), 40-49(n=38), 50-60 (n=23)years old] from July 2018 to July 2019. The erectile function of the patients was evaluated by the international erectile function scale (IIEF-5). The symptoms of hostility, anxiety, psychotic, horrible, paranoid, obsession, somatization, interpersonal relationship and depression were evaluated by SCL-90. Furthermore, STAI was used to distinguish whether the patients had state or trait anxiety. Then EPQ was used to analyze the personality types of patients. The differences of SCL-90, STAI and EPQ between the patients and the national norm group were analyzed by the two sample t-test. The variance analysis was conducted to compare the score differences of scales among different age groups of ED patients. The chi square test was conducted to compare the distribution differences of personality types among different age groups. Results:The scores of hostility(1.64±0.67, t=4.81, P<0.001), anxiety(1.58±0.66, t=6.83, P<0.001), psychotic(1.62±0.68, t=11.87, P<0.001), paranoid(1.55±0.66, t=3.58, P=0.0004), obsession(1.95±0.70, t=9.56, P<0.001), somatization(1.43±0.58, t=2.10, P=0.036), interpersonal relationship(1.74±0.74, t=2.79, P=0.005), depression (1.66±0.74, t=4.50, P<0.001)and the total scores (1.53±0.63, t=3.07, P=0.002)of SCL-90 in the patient group were significantly higher than those of the national norm group, and there were significant differences in the scores of interpersonal relationship among different age groups(1.72±0.78, 1.65±0.69, 1.58±0.92, 1.43±0.59, F=2.84, P=0.038). The scores of state anxiety( t=7.35, P<0.001), trait anxiety ( t=6.31, P<0.001)and the total scores ( t=8.41, P<0.001)of STAI in the patient group were significantly higher than those of the national norm, and there were significant differences in the scores of state anxiety( F=5.29, P=0.001), trait anxiety ( F=5.54, P<0.001)and total scores ( F=5.66, P<0.001)among different age groups. There were significant increases in the scores of psychoticism( t=30.56, P<0.001), emotion( t=45.94, P<0.001), extraversion and introversion( t=11.72, P<0.001), concealment factors ( t=29.16, P<0.001)and total scores ( t=30.56, P<0.001)in the patient group. The proportion of depressive personality was highest in the ED patients(n=183; 45.64%), but there was no significant difference in the distribution of personality types among different age groups[20-29(depressive, choleric, mucinous and sanguine): 76, 35, 26, 21; 30-39: 87, 40, 32, 23; 40-49: 14, 10, 6, 8; 50-60: 6, 9, 2, 6; χ 2=10.65, P=0.30]. Conclusions:ED patients have a series of abnormal emotions, somatized discomfort, sensitive interpersonal relationships, introverted and unstable emotionally personality characteristics. In addition, the younger the patients are, the more serious their anxiety are, the more sensitive their interpersonal relationships are, which may be related to their introverted personality characteristics and emotional instability.

Objective Cyclin D1 gene plays a significant role in regulating cell cycle progression.It is reported that over-expression of cyclin D1 gene is intimately associated with origination,development and prognosis of tumor and is associated with tumor cells resistance to chemotherapy drug.Suppression of cyclin D1 protein expression leads to cellular chemosensitization.This study was to determine whether this effect also existed in chronic leukemia cell line K562 by inhibiting the expression of cyclin D1 protein through RNA interference.Methods Plasmid vectors expressing small hairpin RNA (shRNA) targeting at cyclin D1 gene were constructed and transfected into K562 cells by chitosan.Cyclin D1 protein was examined using Western Blotting analysis.The cell cycle and apoptosis were determined by flow cytometry.Cellular chemosensitization was evaluated by MTY assay.Results Expression of cyclin D1 protein was markedly down-regulated after transfection with pshRNA-419 and pshRNA-575 at 48 h.Down-regulation of cyclin D1 protein could affect the redistribution of cell cycle,induce apoptosis of K562 cells,decrease 50％inhibitoryconcentration (IC50) of adriamycin and enhance cellular chemosensitization.But there had no above biological effects observed after transfection with blank vector and control vector of m-pshRNA-790 at 48 h.ConclusionK562 cells could be chemosensitized by the down-regulation of cyclin D1 expression through RNA interference.

Objective: To develop a new determination method of astrogaloside in s erum by spectrophotofluorimetry. Methods: The fluorescence char acteristics of astragaloside anisic reaction product were used to determine astragaloside in serum. Re sults: The reaction product of astrogaloside and anisic acid had excitatio n and emission maxima were at 320 and 387 nm, respectively. The linear range of determ inati on was between 0.2 and 40.0 mg*L －1. The detection limit was 0.02 mg*L －1.　The recovery of measurement was between 98.8% and 102.4%. Conclusion: The propos ed method has advantages of high sensitivity, low detection limit and no interference. It has been used to determine astragaloside in serum successfully.

Objective To evaluate the clinical effect of laparoscopy in the modified nerve painesparing radical hysterectomy (NPSRH) of early stage cervical cancer.Methods From January 2014 to December 2015 in our hospital,38 patients with early cervical caucer were enrolled and randomly divided into NPSRH group and laparoscopic radical hysterectomy (LRH) group in the study.The surgery general situations of two groups were compared,and postoperative intestinal and bladder function of these patientswere also assessed.Results All of 38 patients were successfully completed the operation,without significant intraoperative or postoperative complications.There were no significant differences in the bleeding volume,pelvic lymph dissection number,cut length of main ligament and sacral ligament,cut length of anterior vaginal wall,and posterior vaginal wall between two groups (P ＞ 0.05).The mean operation time in NPSRH group was longer than in LRH group,but the duration of postoperative hospital stay was shorter than the latter (P ＜ 0.01).Compared to control group,the catheter retention time,residual urine volume,maximal micturition volume,and Qmax in the NPSRH group recovered better,and with lower rate of nocturia mnd dysuria (P ＜ 0.05).The exhaust time and anal defecation time in the NPSRH group were significantly shorter than the LRH group,and lower rate of constipation additionally (P ＜ 0.05).Conclusions The laparoscopy in the modified nerve paine-sparing radical hysterectomy is a safe and effective measurement for the treatment of early-stage cervical cancer patients.It can significantly reduce the duration of postoperative hospital stay,promote the functional recovery of bladder and rectum,and improve the patients'postoperative quality of life.

Objective To compare the efficacy and safety of ivermectin with metronidazole in patients with intestinal acariasis.Methods 24 patients with typical intestinal acariasis were selected and divided into two groups,each group had 12 members.Double-blind,randomized clinical trial of ivermectin versus metronidazole was assigned.Results 24 patients completed 3 courses.The total cure rate of ivermectin and metronidazole were 100%(12/12) and 25%(3/12),respectively.There was statistical difference between the two groups(P

To study the photosynthetic physiological characteristics of Cultivars Prunus Humilis bge. in the region of shandong, analyze on the relationship between the quality of seeds and photosynthesis, In order to formulate reasonable cultivation measures and improve the yield quality of seed production. LI-6400 portable photosynthesis apparatus was used to determine light response curve, diurnal change of photosynthetic parameters of Huadong 1 and Huadong 2. Using vernier caliper and electronic balance to measure the quality data of two varieties of eucalypis, the quality difference was analyzed. Two kinds of Prunus Humilis bge, cultivating in Shandong, had higher photosynthetic rate and higher light saturation point, and the photosynthetic rate were "unimodal" type curve. The change of the two peaks all appeared at11: 00, and had no obvious "lunch break" phenomenon. The photosynthetic rate, the utilization of strong and weak light, and the efficiency of absorption and conversion of light energy of Huadong 2, were all higher than that of Huadong 1, which had better quality. The quality of seed of Prunus Humilis bge is related to its photosynthetic capacity. When planting Prunus Humilis bge., we should formulate reasonable cultivation measures according to its features such as photosynthesis, transpiration, water use efficiency.

Objective To understand the dynamic rules of schistosomiasis endemic situation before and after reaching the criteria of schistosomiasis transmission controlled or transmission interrupted,so as to provide the evidence for improving schis-tosomiasis control. Methods Wuling District,Xihu District and Linli County were selected and investigated retrospectively to collect the schistosomiasis epidemiological information 10 years before they reached the criteria of transmission controlled and the subsequent years until 2008. A database of retrospective investigation was established for analyzing the trends and rules of changes of the Oncomelania hupensis snail status and infection status of cattle and human. Results In Wuling District,the en-demic situation was declining,and no schistosome infection persons,animals and snails were found after 1974. There was no re-bound until 2008. In Xihu District,the endemic situation reached the criteria of transmission controlled in 1997,and the endem-ic situation was stable. The human infection rate was positively correlated with the area of infection snails(r=0.584,P<0.05). In Linli County,there were no snails,no infected persons and cattle twice,but 2 endemic rebounds,and there were positive corre-lations between the densities of living snails and the infection rates of human and animal during the endemic rebound period. Conclusion The snail status is an important indicator of schistosomiasis endemic rebound. Therefore,the snail control is one of the most important schistosomiasis control measures.