Objective To discuss the nursing effect and application value of refinement process of nursing management mode in hospital infection control in the operating room.Methods 620 patients who will carry out surgi-cal treatment were selected as the research subjects.310 cases without detailed process of nursing management were included in the control group,and 310 patients with refinement process of nursing management were selected as obser-vation group.The clinical nursing effects of two groups were recorded.Results Observation group surface 88.94%, medical staff hand 92.54%,disinfectant 95.65%,100.00% sterile items.The control surface 81.38%,80.00% of medical personnel hand,disinfectant 75.93%,90.00% sterile items,the monitoring qualified rate between two groups had statistically significant differences (χ2 =5.021,4.596,7.581,6.725,all P <0.05).The sores of nursing safety, operating room management,form filling,ward nursing in the observation group were (95.37 ±4.66 )points, (94.27 ±5.21 )points,(96.13 ±3.29)points,(95.03 ±4.87)points.Those in control group were (90.12 ± 2.03)points,(89.13 ±2.26)points,(90.03 ±1.88)points,(88.17 ±2.25)points,the differences between two groups were statistically significant (t =18.185,15.935,28.343,22.514,all P <0.05).The scores of nurses and patients communication,nursing procedures,ward environment,care attitude in the observation group were (24.19 ± 0.87)points,(24.03 ±0.64)points,(24.51 ±0.45)points,(24.28 ±0.36)points.Those in the control group were (21.54 ±1.65 )points,(20.88 ±1.72)points,(20.95 ±1.66)points,(21.09 ±0.89)points,the differences between two groups were statistically significant (t =25.013,30.220,36.443,58.502,all P <0.05).Conclusion Refinement process of nursing management mode application helps to control nosocomial infection in operating room of hospital infection control,improve the quality of clinical nursing services,improve the clinical nursing satisfaction, which is worthy of popularization and application in clinic.

Objective To investigate the expression of long-chain non-coding RNA gastric cancer high expression transcription factor 1 (GHET1) in hepatocellular carcinoma (HCC) and the correlation with prognosis,cell proliferation,migration and invasion.Methods 20 HCC patients who underwent surgery from Fuzhou General Hospital of Chinese People's Liberation Army from March to May 2016 were included.The HCC tissue and adjacent normal tissue of 182 patients from June 2012 to December 2013 were retrospectively collected.According to the median value of GHET1 expression,it was divided into GHET1 high expression group and low expression group,91 cases each.Huh7 and HepG2 cells were divided into:blank control group (Con) with serum-free medium,siRNA-GHET1 group transfected with siRNA-GHET1,and negative control group (siRNA-NC) transfected with negative control sequence.The expression of GHET1 was detected by real-time fluorescence quantitative polymerase chain reaction,and the effect of GHET1 on HCC cells was analyzed by CCK-8,Transwell assay and Western blot.Results Compared with adjacent normal tissue,the relative expression of GHET1 mRNA in HCC tissues was significantly increased.Compared with LO2 cells,the mRNA expression of GHET1 in Huh7 and HepG2 cells was higher (P＜0.05).The GHET1 high expression group had tumor＞5 cm,vascular invasion,AFP＞400 μg/L,Edmonson grade Ⅰ,and the tumor-free ratio was lower in the expression group (P＜0.05).Survival analysis showed that HCC patients with high GHET1 expression had a poorer prognosis than patients with low expression.Multivariate Cox regression analysis showed that high expressed GHET1,vascular invasion (HR=2.067,95％ CI:1.350 to 3.162),and without tumor capsule are independent predictors of recurrence in HCC patients.After transfection with Huh7 and HepG2 cells,the proliferation of siRNA-GHET1 group was significantly decreased comparing with Con and siRNA-NC groups.Compared with siRNA-NC group,the migration and invasion ability of siRNA-GHET1 group decreased,and E-cadherin expression increased.The expression of fibronectin and vimentin decreased,and the difference was statistically significant (P＜0.05).Conclusions The expression of GHET1 in HCC tissue is higher comparing with normal tissue,which increases the proliferation,migration and invasion of hepatoma cells.It is an independent predictor of prognosis in HCC patients and a potential target for clinical treatment.