1.Application of antibody cocktail method in the immunohistochemistry.
Hong YANG ; Ke LI ; Dan-dan DONG
Chinese Journal of Pathology 2005;34(3):182-183
Actins
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immunology
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metabolism
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Antibodies, Monoclonal
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analysis
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Antigens, CD34
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immunology
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metabolism
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Breast Neoplasms
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metabolism
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Female
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Humans
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Immunohistochemistry
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methods
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Lung Neoplasms
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metabolism
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Membrane Proteins
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immunology
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metabolism
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Tumor Suppressor Protein p53
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immunology
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metabolism
2.Effect of low concentration Aβ1-42 monomer/oligomers and CORM-2 on livability of SN56 cells
Man JIN ; Peiyuan LU ; Ling LI ; Yanghong DONG ; Hongjuan YUE ; Jinling LI ; Mingyue FAN
Chinese Journal of Behavioral Medicine and Brain Science 2010;19(12):1096-1098
Objective To observe the effect of low concentration Aβ1-42 monomer/oligomers and CORM-2 in different concentration on livability of SN56 cells. Methods SN56 cells were cultured in the 96-well plate with uniform concentration, and were divided into control group, Aβ1-42 group, Aβ1-42 + CORM-2 50μM group, and Aβ1-42 + CORM-2 100 μM group. Three lines of cells in Aβ1-42 group were cultured in the surroundings of 10nM,100nM and 1 μM Aβ1-42monomer/oligomers, respectively. Aβ1-42 + CORM-2 50μM group and Aβ1-42 + CORM-2 100μM group had the same culture condition as group Aβ1-42 ,except contain 50μM, and 100μM CORM-2, respectively. Control group didnt have any effect factor. Three days later,the livability of different groups was compared with MTT method. Results The livability of group Aβ1-42 with the increasing concentration of Aβ1-42 was (79.73 ±0.94)% ,(67.99 ±0.79)% ,(60.42 ±0.62)% , respectively. The higher the concentration of Aβ1-42 was,the lower the livability of SN56 cell was. The livability of group Aβ1-42 + CORM-2 50μM/100μM was( 75.15±0.096)%,(63.20 ±0.17)%, (55.33 ±0.19)%; (73.20 ±0.27)%, (64.34 ±0.11 )%, (54.17 ±0.12)% , respectively. Both were lower than group Aβ1-42. And different CORM-2 concentration had discrepancy in the ability of decreasing the cell livability. Conclusion Low concentration of Aβ1-42 can reduce the livability of SN56 cells, and higher concentration has more significant effect; CORM-2 in different concentration both can decrease the livability of SN56 cells,and there is a discrepancy in the intensity.