A comparative observation about histological and histochemical study were made onthe preservating methods of local ischemic kidney of dogs.The methods of this exp-eriment were divided into five groups:A.Local hypothermia;B.Hypothermic perfu-sion solution 500ml,4℃,containing Procaine 450 mg and Heparin 100 mg,infusedinto renal artery;C.Hypothermic perfusion solution 500ml,4℃,containing 20% Ma-nniton 32ml,25% MgSO_4 0.36 ml,Heparin 100 ml,infused into renal artery;D.Localhypothermia and heparin 30 mg injected into the same vessel.E.Local room tem-perature.The specimens were taken from the kidneys of the experimental dogs atvarious period after treated with every method.The results were as follows;A,D and E group presented irreversible histological changes within 4 hoursaftertreatment.Four hours later,the reaction of SDH,ATPase,AlPase werenegative.It suggested that the kidney damage was serious,as well as expressed thatthe effect of these preservating methods for ischemic kidney are not satisfactory.In B group,the histological and histochemical alteration is very light and almostreversible,during 60 days after treatment.In C group,hypothermic perfusionsolution containing Mannitol,MgSO_4 etc either during experiment or follow observa-tion for 60 days,no histological and histochemical alterations were found.It indica-ted that this method is an ideal perservating technique for ischemic kidney.

Objective: To investigate the epidemiological characteristics and the causes of two outbreaks of upper respiratory tract infection in our city. Methods: The epidemiological investigation was carried out on the outbreaks and the pharyngeal swabs were collected to do the pathophysiologic examination by using real-time PCR. Hexon gene of adenovirus was amplified by PCR. Then the positive products were sequenced and BLAST searching was done in GenBank. Results: The clinical symptoms of the two cases were high fever, sore throat and cough, etc, while the main signs were pharyngeal congestion and tonsil swelling; 32 students of a class in Yunxing primary school had respiratory infection related symptoms, of which the incidence rate was 55%; while 12 of 38 students in a class of Fangcun primary school were infected. All these students were on school in the same class, and the incidence was aggregate without proliferation. The duration of the disease was about 5 days and the prognosis was good, no death occurred. The positive result of real-time PCR showed adenovirus. BLAST search analysis on hexon gene showed AdV14. Conclusions According to epidemiological investigation and laboratory test results, the cause of these two outbreaks of upper respiratory tract infection was adenovirus type 14.

Objective To explore the effect of newly graduated nurses'(NGRNs) career adaptability on transition shock .Methods Career adapt-abilities scale ( CAAS ) and transition shock of NGRNs scale were used to investigate career adaptability and transition shock of 223 NGRNs in 6 third-rate hospitals of Hu′nan Province , and their correlation was analyzed .Results Average score of dimension of career confidence was the highest(3.61 ±0.53) in career adapt-abilities while the dimension of career control was the lowest (3.29 ± 0.74).In transition shock scale, the average of health condition was the highest (3.72 ±0.84) while the social culture and development was the lowest (3.24 ±0.76).Career adaptability of NGRNs was negatively related to transition shock (r=-0.440,-0.224,-0.258,-0.238;P<0.05).Multiple stepwise regression analysis indicated that career concern , career curiosity , career control and career confidence had significance influence on newly graduated nurses′transition shock .Conclusions Hospitals managers should focus on career concerns and control of NGRNs and take measures to improve NGRNs′career adaptability , help them finish role transition smoothly to stabilize the nursing team and improve nursing quality finally .

Objective To investigate the prevalence,subtypes and infected population of influenza (flu) viruses in Xuzhou city from 2010 to 2015,providing scientific supports for influenza prevention and control.Methods Molecular detection and virus isolation were carried out with the throat swab samples collected from influenza-like illness(ILI) cases of national sentinel hospitals and the flu outbreaks.Results The influenza has obvious epidemic characteristics seasonally,two concentrated outbreaks in every December to next March and August to September.The positive rates were different among age groups,the highest was in less than ten years old group (64.36％),then 10-19 years old group (10.69％),and the lowest was in 50-59 years old group (3.77％).A total of 15 245 swab specimens were collected in which 1 459 strains were detected and identified for influenza viruses with total positive rate of 9.57％.Among these positive samples,593 samples were H3 subtype (40.64％),627 samples were B subtype (42.97％),237 samples were H1 N1 subtype (16.24％).Four hundred and fouty strains were isolated fromMDCK cells the relatively isolate rate was 30.16 ％ (440/1 459),fifty nine strains from embryonated chicken (13.78％).Conclusions The epidemic types of influenza in Xuzhou from 2010 to 2015 were alternation between H3 and B.There were epidemic peaks of H1N1occurred occasionally and unstably.

Objective:To detect the expression of methyl methanesulfonate and UV sensitive gene clone 81 (Mus81) in hepatocellular carcinoma(HCC) and to observe the effects of Mus81 on the migration, invasion and metastatic ability of human HCC cells.Methods:Thirty-two tissue specimens were selected from HCC tissues and corresponding paraneoplastic tissues of patients with HCC who underwent surgical resection in Guangzhou Red Cross Hospital Affiliated to Jinan University from January 2020 to June 2021. The expression levels of Mus81 in 32 HCC specimens, 374 HCC samples from the cancer genome atlas database, human normal liver cell line HL-7702 and human HCC cell lines JHH-7, Huh-7 and Hep3B were analyzed. Mus81 knockdown in JHH-7, Huh-7 and overexpressed in Hep3B HCC cell lines were constructed, and the effects of Mus81 on HCC cells were observed by scratch assay, Transwell migration and invasion assay and tail vein injection transfer assay in nude mice.Results:The expression of Mus81 was higher in HCC tissues or cell lines than which in paraneoplastic tissues or normal hepatocyte lines (all P<0.05). The migration rate, metastatic and invasive cell numbers of Mus81-knockdown Huh-7 HCC cells were 22.24%±2.16%, 49.04±5.62, 3.81±1.08, the negative control group were 26.89%±1.15%, 86.81±4.79, 19.78±3.30, and the differences between the two groups were statistically significant ( t=4.24, 26.59, 23.92, all P<0.01). The migration rate, metastatic and invasive cell numbers of Mus81-overexpressed Hep3B HCC cells were 80.57%±5.12%, 18.74±8.07, 33.81±8.44, which were significantly higher than those of the empty vector group 64.17%±7.20%, 10.96±5.32, 3.04±1.13, and the differences were statistically significant ( t=4.15, 4.18, 18.78, all P<0.01). Tail vein transfer experiments in nude mice showed that the total fluorescence expression, weight of metastatic tumors, and the metastatic rates in kidney, vertebral column, neck, axilla and subcutis in nude mice injected with Mus81-knockdown JHH-7 cells were significantly lower than those in the control group (all P<0.05). Conclusion:Mus81 gene expression is upregulated in HCC and promotes the migration, invasion and metastatic ability of HCC cells, suggesting that Mus81 may be a potential therapeutic target for HCC.

Objective:To understand the epidemiological characteristics of human adenovirus(HAdv)in Xuzhou area.Methods:A total of 7 257 swab samples from influenza-like illness children in Xuzhou children’s Hospital were collected during 2013-2019. Real-time PCR was used to detect adenovirus, and then the positive specimens were subjected to amplification for the hexon gene by polymerase chain reaction (PCR) for genotypic analysis. Adenovirus 55 was cultured by Hep-2 cells and analyzed by whole gene sequencing.Results:A total of 227 strains of adenoviruses were detected out of 7 257 samples, and the detection rate was 3.13%. Infections were mainly found in children aged 3 to 6, with a peak of detection in May and June. Typing data showed that the infection was mainly adenovirus type 3 and type 7, and the dominant type of adenovirus in different years alternated between type 3 and type 7. Complete gene sequencing showed only a few point mutations in genes of adenovirus type 55 we isolated, and no other gene mutation and recombination were found.Conclusions:The main types of adenovirus were types 3 and 7 among children with inluenza-like illness in Xuzhou from 2013 to 2019, and the infected population was mainly preschool children, and the detection peak was in spring and summer.