OBJECTIVE To compare the detection rates of Ureaplasma urealyticum(Uu),by 4 kinds rits and find out the best one.METHODS Using four kinds of mycoplasma detection kits,namely are French mycoplasmaIST2,Zhuhai Livzon′s ICS,Dark horse′s IES and Shanghai Its Prachanda,under the same conditions,the mycoplasma of the Clinical specimens for 260 cases were tested.RESULTS The detection rate of mycoplasma(Uu) for 48 hours by four kinds of brands kits about mycoplasma liquid culture was as follows 45.4% for French MycoplasmaIST2,36.2% for Zhuhai Livzon′s ICS,37.7% for Dark horse′s IES and 36.8% for Shanghai Its prachanda.The results of susceptibility test: the reagent of IST2 had.the lowest resistance to josamycin,doxycycline,clarithromycin,ofloxacin and azithromycin,and had the better results than the other three kinds of reagents.Mycoplasma reagents form different companies had significant differences in resistance rates to five kinds of antibiotics.CONCLUSIONS The results suggest that when we cultivate clinical mycoplasma should be careful to select of the mycoplasma liquid culture identification kit.

Objective To study the effect of long‐acting nitrate on cardiac function and expression of AngⅡreceptor (ATR)subtypes in kidneys of chronic heart failure (CHF)rats after myocardial infarction .Methods Ninety male Wistar rats aged 10 weeks were randomly divided into control group (group A ,n=9) ,sham operation group (group B ,n=8) ,HF model group (group C ,n=9) , low Elantan dose group (group D ,n=9) ,high Elantan dose group (group E ,n=9) ,olmesartan group (group F ,n=9) ,and combined high Elantan dose and olmesartan group (group G ,n=8) .A HF model was established by ligating the left anterior descending artery .The animals received gastric drugs for 6 weeks .Their cardiac function was assyed by ultrasound echocardiography and expressions of AT1 R and AT2 R were detected by RT‐PCR and Western blot ,respectively .Results The PRA and AngⅡexpression levels were significantly higher ,the AT1 R expression level was significantly higher and the AT2 R expression level was significantly lower in group C than in group B (P<0 .01 ,P<0 .05) .The PRA and AngⅡexpression levels were significantly lower ,the AT1 R expression level was significantly lower and the AT2 R expression level was significantly higher in groups E‐G than in group C (P<0 .05 ,P<0 .01) .The receptor expression levels were much higher in group G than in group F (P<0 .05) .Conclusion Long‐term use of long‐acting nitrate can effectively improve cardiac function and protect renal function .

Objective To observe the effect of red light facial synthesis combined with comprehensive facial therapy on corticosteroid dependent dermatitis (hormone dependent dermatitis) and summarize the nursing strategies. Methods About 160 patients with hormone dependent dermatitis hospitalized in our hospital from June 2015 to March 2016, were equally divided into control group and study group:the control group was treated with conventional comprehensive therapy, while the study group with 30 min red light irradiation together with the nursing measures in the control group. The total symptom scores of the two groups were evaluated before treatment and 4 weeks after treatment. Results Before treatment, the total symptom scores of the control group were no significant difference between them (P>0.05). After treatment,the treatment effect of the study group was significantly better than that of the control group and the scores of ISS SSRI were lower than those of the control group . Conclusion The red light radiation combined with comprehensive facial therapy is effective in the treatment of corticosteroid dependent dermatitis and related nursing should be done well to promote patients relovery.

Objective To analyze the genotype and plasmid transfer of Enterobacteriaceae carring blaNDM‐1 with blaIMP‐4 or blaKPC‐2 .Methods From April 2012 to October 2014 ,a total of 33 non‐repeatitive carbapenem‐resistant Enterobacteriaceae ( including Imipenem‐resistant , meropenem‐resistant or Ertapenem‐resistant) were isolated from 5 hospitals in Wenzhou and Hangzhou . Identification and antimicrobial susceptibility test were performed using Vitek 2 Compact automatic microbiology analyzer . Phenotypes of carbapenemase were screened using modified Hodge test and ethylenediamine tetraacetic acid‐disk synergy test .Extended spectrum βlactamase test was determined by the double disk combination test which was recommended by Clinical and Laboratory Standards Institute .AmpC activity was tested by a three‐dimensional Cefoxitin method .Drug resistant genes including blaNDM‐1 and linkage of ISAba125‐NDM were detected by polymerase chain reaction (PCR) .The purified PCR products were cloned and sequenced .Plasmid conjugation experiment and elimination method were carried out to test partial bacterial strain and K . pneumoniae carrying blaNDM‐1 with blaIMP‐4 or blaKPC‐2 .Results Of the 33 non‐repeatitive carbapenem‐resistant Enterobacteriaceae ,28 were strains of K .pneumoniae ,1 strain of K . oxytoca,2strainsof Escherichiacoli,1strainof K.planticolaand1strainof E.cloacae.Thirteenstrains were isolated from Hospital of Sir Run Run Shaw of Zhejiang University ,thirteen from Wenzhou Hospital of Traditional Chinese Medicine ,one from Wenzhou People′s Hospital ,three from the First Affiliated Hospital of Wenzhou Medical University and three from Wenzhou Hospital of Integrated Traditional Chinese and Western Medicine .Thirty‐one strains were confirmed as carbapenemase‐producing with 24 of blaKPC‐2 ,3 of blaNDM‐1 ,1 of blaNDM‐5 and 3 of blaIMP‐4 .Among them ,one strain carried blaNDM‐1 with blaIMP‐4 and one strain carried blaNDM‐1with blaKPC‐2 ,respectively .The plasmid transfer and conjugation experiment was performed between strains carrying blaNDM‐1 and Escherichia coli EC600 or K . pneumoniae ATCC13833 and genes of blaNDM‐1 and ISAba125‐NDM were obtained .Conclusions blaKPC‐2 gene is the popular carbapenemase genotype .blaNDM‐1 or blaNDM‐5 may be correlated with linkage gene of ISAba125‐N DM .Coexistence of blaNDM‐1 carrying blaIMP‐4 or blaKPC‐2 is detected in the same strain , respectively . Enough importance should be attached to the strains ,because most of them are multiple drug resistance with related genes located in the plasmid which is easily spread between strains .