Circulating tumor cells (CTCs) play pivotal roles for monitoring the tumor metastasis and prognosis.The nanotechnology provides a favourable platform for CTCs detection,and enables CTCs to be more promising for practical application.Meanwhile,the nanoscale device by virtue of nanotechnology has broad application prospects in eliminating CTCs and offers a new direction in the field of anti-cancer.

Objective To verify and evaluate the efficacy and safety of PEG-4000 electrolyte lavage solution in endoscopy preparation. Methods A multicenter open randomized controlled clinical study of 211 patients taking PEG-4000 electrolyte or mannitol in colonoendoscopy preparation. The overall evaluation of colonic preparation was the primary efficacy criterion. The investigator recorded any adverse event to assess the safety. Results The total effective rate of overall colonic preparation of study group was 92. 45% (98/ 106) , that of mannitol group was 80% (84/105). The study group showed more effective than control group with statistical significance (P = 0.016). The adverse event incidence of study group was 8.49%. There were 6 patients complained of nausea and vomiting, 3 patients showed abnormal laboratory results after administration. All the events relieved in short period and have no influence on examination. There was no serious adverse event in this group. The incidence of adverse event in mannitol group was 14. 29% (15/105 ). Four patients showed gastrointestinal symptoms and 1 showed chest suppression. Abnormal laboratory results were found in 10 patients. There was 1 serious adverse event occurred in control group. The difference between 2 groups had no statistical significance. Conclusion PEG-4000 electrolyte is a safe and effective drug in colonic preparation before clinical colonic examination or surgery.

OBJECTIVE:To investigate the prevention and treatment effect of Xiaochaihu decoction on irinotecan(CPT-11)-in-duced bloodystool in model mice with delayed diarrhea. METHODS:40 mice were randomly divided into normal control group (equal volume of normal saline),model control group(equal volume of normal saline),Xiaochaihu decoction group(1500 mg/kg, calculated by crude drug)and loperamide group(positive control,0.5333 mg/kg),ig,once a day,for 17 days. Except for normal control group,other groups were intraperitoneally injected CPT-11 to induce delayed diarrhea in 4th-10th day. Body mass changes, bloodystool rate of mice were determined,and pathological changes in large intestine and small intestine were observed. RE-SULTS:After 17 days of administration,compared with normal control group,the body mass in modeling groups was obviously declined (P<0.05),while body mass in Xiaochaihu decoction group was higher than model control group (P<0.05). In the 11th-17th day,bloodystool rate in Xiaochaihu decoction group and loperamide group were significantly lower than model control group(P<0.01),and bloodystool rate in Xiaochaihu decoction group was lower than loperamide group(P<0.05). Pathological re-sults showed,obvious diffuse necrosis and inflammatory cell infiltration were observed in mucosal epithelial cells of large intestine and small intestine in model control group,and the intestinal mucosal tissue in administration groups tended to be normal. CON-CLUSIONS:Xiaochaihu decoction can effectively reduce bloodystool rate,improve intestinal mucosa injury,and has prevention and treatment effect on CPT-11-induced bloodystool in mice,with superior effect to loperamide.

Objective To evaluate the efficacy of preoperative transarterial chemotherapy for invasive bladder cancer. Methods A total of 60 cases of invasive bladder cancer have been confirmed on open surgery and pathological studies were retrospective studied.The cases were allotted to preoperative transarterial chemotherapy group (30 cases) and operation alone group (30 cases).Preoperative transarterial chemotherapy was performed by infusing anticancer drugs into internal iliac artery.Surgery was performed in 1～2 weeks after transarterial chemotherapy.The bladder preservation rate,recurrence rate,metastasis rate and survival rate were compared between the 2 groups. Results In the preoperative transarterial chemotherapy group,the bladder preservation rate was improved and the recurrence rate dropped (P0.05). Conclusions Preoperative transarterial chemotherapy is a safe and effective method in the treatment of invasive bladder cancer.

Recurrence and metastasis is the main cause of death in breast cancer patients. Early detection and treatment of malignant breast cancer cells and metastasis are the hotspots of current resear-ches. The discovery of circulating tumor cells(CTC)may provide new ideas for the diagnosis and treatment of breast cancer. Detection of CTC will help the early detection of micrometastasis,the guidance of individ-ual treatment,and the evaluation of therapeutic effect and prognosis. Domestic and foreign research pro-gress of CTC was reviewed in this paper.

Objective To study the sub chronic toxicity of silver nanoparticles on medaka.Methods Adult fish were divided into silve nenopartides and control group.Animals were collected on 14 days after exposure, and some toxicological endpoints such as death rate, tissue distributed of silver irons, oxidative stress and histopathological damage were measured.Results There were significant difference in death rate of medaka treated with silver nanoparticles and control group.Compared with the control group, the content of silver in gill, intestine and liver of medaka treated with silver nanoparticles were increased significantly.Compared with control group, the activity of LDH in liver and SOD in liver and gill were significantly decreased(P<0.01).The content of MDA in liver of medaka treated with silver nanoparticles was significantly increased(P<0.01).The liver and gill of mekada treated with silver nanoparticles were damaged, compared with control group.Conclusion Nano silver has a certain subchronic toxicity to aquatic life.

A quail-origin subtype of the influenza virus was isolated from a human-infecting H7N9 subtype of the avian influenza virus found in a live poultry market and was given the name A/Quail/Hangzhou/1/ 2013 (H9N2). We analyzed the whole genome of this virus and its biologic characteristics. Sequence analyses suggested that the: HA and NS genes belonged to a CK/BJ/1/94-like lineage; NA, NP, PA and PB1 genes belonged to a SH/F/98-like lineage; M and PB2 genes belonged to a G1-like lineage. Analyses of key amino acids showed that the cleavage site in HA protein was PSRSSR ↓ GL, and that the HA protein had a human receptor-binding site with Leu226. Deletion of amino acids 69 - 73 was detected in the stalk of NA protein, the M2 protein had an Asn31 mutation, and the NS1 protein had two mutations at Ser42, Ala149. The intravenous pathogenicity of this virus was 0.36. A study in chickens suggested that all inoculated birds shed the virus from the trachea and cloaca on the third day post-infection (p. i. ) until 11 days. All chickens that had direct contact shed the virus on the second day p. i. until 8 days. Results of virus reisolation suggested that lung and tracheal tissues could shed the virus in 5 days, whereas the other organs could shed the virus in 3 days. These results suggest that this virus strain is H9N2 subtype LPAIV, whose lineage is prevalent in mainland China. This research provides evidence on how to monitor and prevent the H9N2 subtype of the avian influenza virus.
Animals ; Chick Embryo ; Chickens ; China ; Genotype ; Influenza A Virus, H9N2 Subtype ; classification ; genetics ; isolation & purification ; Influenza in Birds ; virology ; Phylogeny ; Quail ; virology

Total caudate lobectomy via anterior hepatic transection is still a new technique to resect the tumor in the caudate lobe,which is mastered only by few surgeons.The procedure was successfully performed on a 21-year old patient with focal nodular hyperplasia in caudate lobe.The right and left lobes were first mobilized,then the short hepatic veins were dissected to detach the caudate lobe from the retrohepatic vena cava.Then the liver was split anteriorly and the partial middle lobe was resected.With this process,the tumor was in the sight and we dissected it from the liver parenchyma.The inflow blood was occluded 3 times with a period of 29,27 and 27 minutes,respectively,with an interval of 5 minutes.The total blood loss during operation was 1000 ml.The patient recovered quickly without any complications.The technique for caudate lobectomy via anterior hepatic transection can improve the success rate and safety of caudate lobectomy and deserve clinical consideration.

Objective To investigate the role of fluid therapy in treatment of septic shock in children.Methods A total of 48 pediatric patients with septic shock were enrolled.Twenty-seven patients were treated with volume expansion and 21 patients were treated with fluid resuscitation.There were no differences in use of antibiotics,inotropic and vasoactive agent in two groups.The time needed for haemodynamic stability,occurrence of pulmonary edema.length of PICU stay,and mortality were compared between two groups.Results Compared with volume expansion group,the time needed for haemodynamic stability significantly decreased in fluid resuscitation group ((216.10± 168.13) minutes vs (121.63 ± 75.59) minutes) (P < 0.05). There were no significant difference in occurrence of pulmonary edema between volume expansion group and fluid resuscitation group (14.8% vs 9.5% . P > 0.05) .Compared with volume expansion group,length of PICU stay significantly decreased in fluid resuscitation group ((6.188 ±3.250) days vs (3.944±2.711) days,P < 0.05).Compared with volume expansion group,mortality significantly decreased in fluid resuscitation group (40.7% vs 14.3%,P < 0.05) . Conclusions Rapid fluid resuscitation was associated with early reach of haemodynamic stability,short stay in PICU,improved survival rate and no increase in the risk of complication in pediatric patient with septic shock.

Totally 1136 individuals aged over 40 underwent health check up in March to May 2009. Fasting blood glucose, 2-h post-challenge blood glucose, glycated hemoglobin A1c ( HbA1c), total cholesterol, triglyceride, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol and serum uric acid were measured. The diagnosis of metabolic syndrome was based on the International Diabetes Federation Criteria. The results shows that total body fat, region body fat indices, blood pressure, blood glucose and serum uric acid levels increased with the age ( P < 0.01) . The prevalence rate of metabolic syndrome in this group of individuals were 15. 1% (171/1136) , and increased with age (P<0.01). The most common combination of metabolic syndrome was central obesity-hypertension-dyslipidemia (40. 9% , 70/171). Unconditional logistic regression revealed that waist-hip-ratio, body mass index and uric acid were the risk factors for metabolic syndrome.