Objective To analysis the hot spots of innovative technology of Chinese medicine industry in Shanghai during the recent years.Methods Patent Database of State Intelletual Property Office was retrieved by IPC class numbers combined with keywords and the IPC classification of the Chinese medicine patents in Shanghai area during 2002-2013 were under statistical analysis.Results The hot spot distribution of therapeutic activity of TCM preparations:Antineoplastic is the most important activity of Chinese medicine development, followed by the activities of treatment of inadequate blood flow or cardiovascular system diseases, such as hyperglycemia, high cholesterol, and so on. The activity of treatment of the digestive tract or digestive system diseases, followed by the activities of anti infection, antiviral, antibacterial, anti-inflammatory treatment, etc. The hot spot distribution of dosage:Researches on new dosage of Chinese medicine were mainly reflected in the improvement of traditional dosage form and development of new formulations. The capsules, tablets, oral liquid, and injections were the new dosage forms under the most researches. The distribution of medical resources:Medicinal plants or animals mainly came from “angiosperm”. Medicinal animals were less than medicinal plants, which mainly belonged to insecta.ConclusionThe hot spots of innovative technology of Chinese medicine industry in Shanghai are mainly distributed in the medicine of anti-tumor, cardiocerebral vascular diseases, digestive system diseases and so on in recent years. The researches of new formulation focus on the traditional dosage form improvement and new formulations development. “Angiosperm” is the key to the development of medicinal plants.

Objective To explore whether the inhibited expression of fibrocystin by RNA interference can increase epidermal growth factor (EGF)-induced cell proliferation and its possible mechanism . Methods A stable PKHD1-silenced HEK 293 cell line was established . Cell proliferation rate, intracellular Ca2+ concentration and extracellular signal-reguhted kinase 1/2(ERK1/2) activity were assessed after treatment with EGF, verapamil and Bay K8644 . Results The proliferation rate of PKHD1-silenced HEK-293 cells was found to be significantly higher after EGF stimulation compared to the control HEK 293 cell (231 .5% vs 152 .8%, P＜0 .01) . PKHD1-silencing lowered the intracellular Ca2+ concentration and caused EGF-induced ERK1/2 overactivation in the cells(P＜0 .01 ) . When cells were treated with verapamil for 4 hours to lower the intracellular Ca2+ concentration, the cell proliferation rate was significantly increased after 20 ng EGF for 24 hours . The verapamil treatment increased the level of activated ERK1/2 in EGF-treated cells . An increase of intracellular Ca2 + in PKHD1-silenced ceils repressed the EGF-dependent ERK1/2 activation and the hyperproliferative response to EGF stimulation . Conclusions Inhibition of fibrocystin can cause EGF-induced excessive proliferation through decreasing intracellular Ca2+ resulting in EGF-induced ERK1/2 activation . The loss of fibrocystin may lead to abnormal proliferation in kidney epithelial cells and cyst formation in ARPKD through modulation of intracellular Ca2+ concentration .

BACKGROUND: The coating induced by conventional simulated body fluid (SBF) and 1.5-folds SBF as biomimetic method has the shortcomings of slowly growth with weeks or months growth cycle. OBJECTIVE: To evaluate Ti-6Al-4V alloy surface deposition of hydroxyapatite coating induced by 3-folds SBF. DESIGN, TIME AND SETTING: In vitro biological analysis. The experiment was performed at the Institute of Metal Research, Chinese Academy of Sciences from November 2008 to March 2009. MATERIALS: Ti-6A1-4V alloy was supported by Boda Metel Materials Co., Ltd. METHODS: The pretreated Ti-6Al-4V alloy was immersed in 37 'C 3-folds SBF. The SBF was replaced every 24 hours for 3 days. MAIN OUTCOME MEASURES: The morphology, phase and elemental composition of calcium phosphate coating were analyzed by scanning electron microscopy, X-ray diffraction and infrared spectroscopy analysis. RESULTS: The scanning electron microscopy exhibited that the surface of Ti-6A1 -4V alloy was covered with deposited film with granular crystal on local areas at day 1 after immersion. The deposited film covered surface of Ti-6A1-4V alloy at day 3 after immersion, and granular or scale-shaped crystal could be seen. The crystal deposition displayed sustained growth with certain area as center. The product comprised Ca, P, O, C elements without Al, V. X-ray diffraction and infrared spectroscopy analysis revealed that the main component of the coating was hydroxyapatite. CONCLUSION: The 3-folds SBF can accelerate the deposition as well as shorten formation duration of hydroxyapatite coating on Ti-6A1-4V alloy.

BACKGROUND:At present, the study to balance ability of functional ankle instability patients is less. The
balance ability deficiency may also be one of the important reasons for ankle repeated injury, so the research can effectively reduce the damage probability.
OBJECTIVE:To explore the static balance ability characteristics of functional ankle instability patients.
METHODS:Ten male subjects with unilateral functional ankle instability were enrol ed as the experimental group in the study, and other 10 healthy match-paired subjects were served as the control group. Their static balance ability was measured under double feet standing and single foot standing using win-pod system, the double feet testing lasted for 30 seconds and the single foot testing lasted for 10 seconds. Evaluation parameters of balance ability were obtained.
RESULTS AND CONCLUSION:During double feet testing, there were no significant differences in balance
ability parameters between the experimental group and the control group whether eyes be opened or closed (P>0.05), but the index values of double feet testing were obviously less than those of single foot testing. So we
concluded that in the double feet standing, there was no significant difference in the static balance ability between functional ankle instability patients and normal persons. Moreover, the static balance ability of double feet was
better than that of single feet. In the single foot standing of the affected foot, the static balance ability of functional ankle instability patients was slightly worse than that of the normal persons, especial y with eyes closed.
Comparison of the average swing between the X-axis and Y-axis found that in the process of maintaining balance posture, the swing direction of the body center of gravity was mainly in the anteroposterior direction.

Objective To explore the effict of Jiegu-Qili tablet combined with intramedullary interlocking nails in the treatment of the fracture of tibia and fibula. Methods A total of 80 patients with fracture of tibia and fibula were selected, and were divided into two groups according to odd and even number of hospitalization. The surgery group (39 patients) was given intramedullary interlocking nails, the surgery and drug group (41 patients) was given Jiegu-Qili tablet combined with intramedullary interlocking nails. The perioperative parameters, the rate of fracture healing, knee scores and complications were evaluated during 12 months. Results The hospitalization (14.43 ± 2.47 d vs. 21.63 ± 3.16 d, t=11.385, P<0.01) and fracture healing time (5.22 ± 1.05 m vs. 9.75 ± 0.97 m, t=20.016, P<0.05) of the surgery and drug group was shorter than that of the surgery group. The fracture healing rate of surgery and drug group (92.7%vs. 76.9%,χ2=3.894, P<0.05) was higher than that of the surgery group. After 6 months surgery, the pain, activity, stability and KSS scores (21.60 ± 1.60 vs. 19.50 ± 1.90, 22.7 ± 2.8 vs.18.70 ± 2.40, 23.50 ± 2.40 vs. 19.40 ± 1.80, 89.20 ± 9.30 vs. 78.00 ± 8.30) of the surgery and drug group were higher than that of the surgery group (P<0.05). There was no statistical significance on walking ability between two groups (21.40 ± 2.50 vs. 20.40 ± 2.20, P>0.05). During 12 months follow-up, there were no statistical significance on complications between two group (7.3%vs.15.4%,χ2=1.303, P>0.05). Conclusions In summary, the Jiegu-Qili tablet combined with intramedullary interlocking nails had a good therapeutic effect on the fracture of tibia and fibula. It could increase the speed of fracture healing, shorten the hospitalization, reduce the postoperative pain and improve the activity of patients.

Objective To investigate the clinical manifestation, pathological features and pathogenesis of Holmes-Adie syndrome (HAS). Methods The clinical data of one HAS patient with sweating abnormality and combined with literatures were analysed. Results The patient had the typical characteristics of HAS, including Adie pupil, areflexia of lower limb and the symptoms of autonomic nerve dysfunction. The characteristic pupil of HAS was proved by slit lamp of ophthalmology. Cranial MRI showed lacunar infarction. A partial or total loss of neurons of the ciliary ganglion was the main pathological characteristic of HAS. Conclusions The etiology of HAS remains unclear, and has characteristic pupil finding, which was associated with autonomic nerve dysfunction to a certain extent. HAS still need to differentiate from Ross syndrome and Harlequin syndrome.

AIM: To prepare the asperosaponin—phospholipid compound to improve the bioavailability of asperosaponin in rat intestine in situ. METHODS: Ratio of asperosaponin to phospholipid was chosen as index to screen the optimal proportion based on the orthogonal design.Rat intestine perfusion was use to compare aspersaponin absorption under with and without phospholipid. RESULTS: Asperosaponin—phospholipid compound showed the markedly improvement in absorption capacity and absorption rate. CONCLUSION: The experiment recommends that asperosaponin in the combination with phospholipid is better than only used asperosaponin concerning bioavailability.

OBJECTIVE: Simulateous determination of ten psychosedatives in serum was made to help doctors make effective rescue. METHOD: The qualitative separation was done by TLC. The quantitative determination was made by TLC scanning. RESULTS: Ten psychosedatives were separated very well by one mobile phase. The recovery, accuracy and stability of TLC scanning on varied wave-lengths were satisfatory. CONCLUSION: This method is simple, rapid, and accurate. It can be used in poison analysis and therapeutic drug monitoring.

Objective To investigate the diagnostic and theraputic problems in patients with smooth muscle tumors of the duodenum.Method The clinical data from 7 cases smooth muscle tumors were analyzed retrospectively which were treated surgically and confirmed pathologically in our hospital from 1987 to 2001.Results Of all the cases,4 had a leiomyoma in the duodenum and 3 leiomysarcoma.This disease is lack of pathognomonic sign and difficult for early diagnosis.All the cases were treated surgically and got good results.Conclusions Upper gastrointestinal bleeding is common in patients with this disease.Upper gastrointestinal tract examination and gastrduodenoscopy and CT scan are the main methods for diagnosis.Operation is the best choice for treatment.

From the soon-to-be-completed Human Genome Project to Combinatorial Chemistry and High-Throughput Screening combined with bioinformatic tools, scie n tific advances are poised to revolutionize drug discovery and even health care. The research directions in the State Key Laboratory of Natural and Biomimetic Dr ugs of Peking University mainly focus on the study of interaction between biolog ical targets and active compounds with structural diversity including endogeno us and exogenous substances, especially the natural products that come from anim als, plants and inorganic medicines. The new lead compounds have been designed and s ynthesized based on the structure-activity relationship and computer modeling. T he research activities are involved in 76 national research projects. From 1996 -20 00, 310 papers have been published, 8 patents obtained an d 14 prizes of scientific achievements awarded by the Chinese government and min istries.