Objective:We had analyzed the attitude of science researchers in a university to promote the development of science cause in the university. Method:We had made a prevalence survey through a questionnaire. Results:Some researchers join in science cause to acquire some profits,they often change their research orientation and they aren’t willing to cooperate with each other. Conclusion:We must initiate a kind of spirit of which advantageous to collaborate,insisting,struggling and seeking the truth of science.

Objective:To find a good culture medium for embryo development in-vitro.Methods:D4 mouse embryo was cultured with carcinoma of kidney GRC-1 growth fluid and FD+20% fetal calf serum respectively in-vitro and the rate of zona-hatching,attachment and trophoblast outgrowth were compared respectively.Results:The rate of zona-hatching,attachment and trophoblast outgrowth with GRC-1 were 89.68%,86.51 %和84.92%,respectively.These were significantly higher than those of the control group ( P

For the particularity of obstetrics and gynecology disease and conventional idea of the people,clinical education of obstetrics and gynecology is very difficult.How to increase the clinical education quality of obstetrics and gynecology and how to increase the clinical'ability of medical students have become an important problem in teaching obstetrics and gynecology.In this article,we have discussed the problems and countermeasures in the teaching of obstetrics and gynecology.

Objective To investigate the protective effect of methotrexate on probable rheumatoid arthritis (RA) patients. Methods 62 probable rheumatoid arthritis (RA) patients were randomly divided into two groups, treatment group (32 cases) accepted methotrexate and non-steroidal anti-inflammatory drug and control group only non-steroidal anti-inflammatory drug. In the 2-year follow-up visit, patients who developed to RA would withdraw and accept normal combined medicine treatment. They were assessed with X-ray changes of the hand joints. Results 8 cases of the treatment group developed RA (2 cases withdrew because of intolerance), while 17 cases of the control group developed RA. The treatment group developed RA later than the control group, with less radiographic joints injury in 2 years. Conclusion Methotrexate can delay clinical process of the development of RA in the probable RA patients and reduce joints damage.

目的探讨加强康复指导对提高患者运动功能和日常生活能力(ADL)的意义。方法选择脊髓损伤患者59例,随机分两组,1组在康复治疗过程中开展康复指导(以下简称康复指导组) ;1组常规康复治疗(以下简称对照组)。康复指导组由专人负责制订实施康复训练,并对患者及家属、陪护人员进行指导。对照组不设专人,由主管医师负责康复训练并提供随机指导。治疗前及治疗后两组患者均采用Barthel指数进行评定。结果康复指导组在患者运动功能、ADL方面明显优于对照组,差异有显著性(P<0.01)。结论脊髓损伤康复中加强康复指导,有助于提高患者运动功能和生活自理能力。

Lysyal oxidase (LOX),a cooper dependent monoamine oxidase,can stabilize the extracellular matrix and is closely related to the tumor cell differentiation,vicious transformation and invasiveness.LOX is secreted into extracellular,and it is resolved into a small fragment by sol protease,which is lysyal oxidase propeptide (LOX-PP).According to research,LOX-PP has its own structure and physiological functions,which can inhibit the growth of tumor cells.LOX-PP also plays a significant role in the generation and progression of tumor,which is likely to be a new therapeutic target.

Mesenchymal stem cells (MSC) possess an innate ability for self-renewal and can differentiate into multiple cell types.MSC have been used in cell-based therapy in various disease conditions including tumors.However,as MSC therapy gained popularity among practitioners and rescarchers,there have been reports on thre adverse effects of MSC especially in the context of tumor modulation and malignant transformation.Recent studies have shown that MSC also play an important role in the resistance to various anti-cancer drugs.This review provides an overview of the functional properties of MSC in cancer drug resistance.

Intracellular calcium is one of the crucial signalings that modulate various physiological and pathological processes.Abnormal calcium signaling is closely associated with invasion and migration of neoplasms.Calcium regulates focal adhesion turnover,cytoskeletal reorganization and other processes about tumor cell movement through contacting with multiple downstream proteins,such as calpain,PYK2 and S100 protein family.Several calcium entry channels including store-operated calcium channels and transient receptor potential channels participate in the invasion and migration of tumor cells.Pathological calcium signaling may be a potential target in anti-invasion and anti-migration therapy.

Invasive fungal infection is an important cause of mortality and morbidity in preterm infants.Extremely preterm and extremely low birth weight infants are at highest risk because of the intensive and invasive nature of the care that these infants receive.How to prevent and treat the invasive fungal infection in preterm infants has become a new hot spot in the neonatologists.This review focused the progress on treatment and prevention of invasive fungal infections in preterm infants.

Objective To investigate the serum levels of anti-BRAF antibody in patients with rheumatoid arthritis (RA) and its clinical significance.Methods Blood samples from 129 patients with RA,71 patients with other rheumatic diseases and 68 healthy controls were measured by enzyme-linked immunosorbent assays (ELISA).The serum levels of anti-BRAF antibody in patients with RA were divided into high,medium and low groups,each group had 43 cases.The clinical symptoms,immunological findings and related imaging data of all cases were recorded.We investigated the relationship between the serum levels of anti-BRAF antibody and the clinical symptoms,immunological findings and related imaging changes of the RA group.Chi-square test,Mann-Whitney U test,Spearman's correlation test were used for statistical analysis.Results ① The serum levels of anti-BRAF antibody were significantly higher in RA group [A:1.093 (0.150-3.210)] than in other rheumatic diseases group [A:0.278 (0.093-0.455)] and healthy controls [A:0.202 (0.121-0.443)] (Z=-11.223,P=0.021 ; Z=-7.839,P=0.008 7).The serum levels of anti-BRAF antibody was not significantly different between patients with other rheumatic diseases group and healthy controls (Z=-0.905,P=0.445).② The difference in positive rate of rheumatoid factor (RF) in different groups of anti-BRAF antibody levels was statistically significant (λx2=10.14,P=0.006),namely the positive rate was significantlyhigher in the low-level group (88％) than in the high-level group (58％) (x2=10.03,P=0.002).Thepositive rate of anti-cyclic citrullinated peptide antibody and anti-keratin antibody of the three groups was not significantly different (x2=0.721,P=0.697; x2=1.735,P=0.188).③ There was significant negative correlation between the levels of anti-BRAF antibody and the course of disease (r=-0.233,P=0.047),RF(r=-2.84,P=0.039),IgM (r=-0.234,P=0.038),and hemoglobin (r=-0.287,P=0.032).There was no significant correlation between the level of anti-BRAF antibody and the joint swelling index (r=-0.133,P=0.230),joint pain index (r=-0.173,P=0.100),other clinical and laboratory parameters.There was significantly negative correlation between the ESR and hemoglobin level.④ There was no significant difference of the level of anti-BRAF antibody between the normal group and patients with bone destruction in the RA group (Z=-0.642,P=0.521).⑤ There was no significant difference in the level of anti-BRAF antibody between the normal group and patients with interstitial pulmonary fibrosis group in the RA group (Z=-0.121,P=0.904).Conclusion The anti-BRAF antibody can be used for the diagnosis of RA as a new autoantibody,and it can also be used in the diagnosis of early or RF-negative RA.There is no correlation between the level of anti-BRAF antibody and bone destruction and interstitial pulmonary fibrosis.