Objective:We had analyzed the attitude of science researchers in a university to promote the development of science cause in the university. Method:We had made a prevalence survey through a questionnaire. Results:Some researchers join in science cause to acquire some profits,they often change their research orientation and they aren’t willing to cooperate with each other. Conclusion:We must initiate a kind of spirit of which advantageous to collaborate,insisting,struggling and seeking the truth of science.

Objective:To find a good culture medium for embryo development in-vitro.Methods:D4 mouse embryo was cultured with carcinoma of kidney GRC-1 growth fluid and FD+20% fetal calf serum respectively in-vitro and the rate of zona-hatching,attachment and trophoblast outgrowth were compared respectively.Results:The rate of zona-hatching,attachment and trophoblast outgrowth with GRC-1 were 89.68%,86.51 %和84.92%,respectively.These were significantly higher than those of the control group ( P

For the particularity of obstetrics and gynecology disease and conventional idea of the people,clinical education of obstetrics and gynecology is very difficult.How to increase the clinical education quality of obstetrics and gynecology and how to increase the clinical'ability of medical students have become an important problem in teaching obstetrics and gynecology.In this article,we have discussed the problems and countermeasures in the teaching of obstetrics and gynecology.

Objective To explore the double-labeling method of in 9itu hybridization(ISH) with cRNA probe and immunohistochemistry in the paraffin sections of the skin.Methods The double-standard coexistence signals by situ hybridization and immunohis- tochemistry in 15 cases with acne and folliculitis were observed.Results The blue-violet positive signals of ISH could be observed in the hair follicle epithelium,sebaceous glands and ducts of paraffin section of skin.Meanwhile,the brown dendritic positive signal also could be seen in the sebaceous glands.Conclusions The use of in situ hybridization with digoxin-labeled cRNA probe combined with immunohistochemistry could locate microbial component and epidermal cells on skin paraffin section exactly.

Objective To investigate the serum levels of anti-BRAF antibody in patients with rheumatoid arthritis (RA) and its clinical significance.Methods Blood samples from 129 patients with RA,71 patients with other rheumatic diseases and 68 healthy controls were measured by enzyme-linked immunosorbent assays (ELISA).The serum levels of anti-BRAF antibody in patients with RA were divided into high,medium and low groups,each group had 43 cases.The clinical symptoms,immunological findings and related imaging data of all cases were recorded.We investigated the relationship between the serum levels of anti-BRAF antibody and the clinical symptoms,immunological findings and related imaging changes of the RA group.Chi-square test,Mann-Whitney U test,Spearman's correlation test were used for statistical analysis.Results ① The serum levels of anti-BRAF antibody were significantly higher in RA group [A:1.093 (0.150-3.210)] than in other rheumatic diseases group [A:0.278 (0.093-0.455)] and healthy controls [A:0.202 (0.121-0.443)] (Z=-11.223,P=0.021 ; Z=-7.839,P=0.008 7).The serum levels of anti-BRAF antibody was not significantly different between patients with other rheumatic diseases group and healthy controls (Z=-0.905,P=0.445).② The difference in positive rate of rheumatoid factor (RF) in different groups of anti-BRAF antibody levels was statistically significant (λx2=10.14,P=0.006),namely the positive rate was significantlyhigher in the low-level group (88％) than in the high-level group (58％) (x2=10.03,P=0.002).Thepositive rate of anti-cyclic citrullinated peptide antibody and anti-keratin antibody of the three groups was not significantly different (x2=0.721,P=0.697; x2=1.735,P=0.188).③ There was significant negative correlation between the levels of anti-BRAF antibody and the course of disease (r=-0.233,P=0.047),RF(r=-2.84,P=0.039),IgM (r=-0.234,P=0.038),and hemoglobin (r=-0.287,P=0.032).There was no significant correlation between the level of anti-BRAF antibody and the joint swelling index (r=-0.133,P=0.230),joint pain index (r=-0.173,P=0.100),other clinical and laboratory parameters.There was significantly negative correlation between the ESR and hemoglobin level.④ There was no significant difference of the level of anti-BRAF antibody between the normal group and patients with bone destruction in the RA group (Z=-0.642,P=0.521).⑤ There was no significant difference in the level of anti-BRAF antibody between the normal group and patients with interstitial pulmonary fibrosis group in the RA group (Z=-0.121,P=0.904).Conclusion The anti-BRAF antibody can be used for the diagnosis of RA as a new autoantibody,and it can also be used in the diagnosis of early or RF-negative RA.There is no correlation between the level of anti-BRAF antibody and bone destruction and interstitial pulmonary fibrosis.

Invasive fungal infection is an important cause of mortality and morbidity in preterm infants.Extremely preterm and extremely low birth weight infants are at highest risk because of the intensive and invasive nature of the care that these infants receive.How to prevent and treat the invasive fungal infection in preterm infants has become a new hot spot in the neonatologists.This review focused the progress on treatment and prevention of invasive fungal infections in preterm infants.

Mesenchymal stem cells (MSC) possess an innate ability for self-renewal and can differentiate into multiple cell types.MSC have been used in cell-based therapy in various disease conditions including tumors.However,as MSC therapy gained popularity among practitioners and rescarchers,there have been reports on thre adverse effects of MSC especially in the context of tumor modulation and malignant transformation.Recent studies have shown that MSC also play an important role in the resistance to various anti-cancer drugs.This review provides an overview of the functional properties of MSC in cancer drug resistance.

Objective:To explore the appearances o f digital subtraction angiograph y (DSA) and therapeutic efficacy of interventional therapy of hepatic carcinoma accompanied with arteriovenous shunting (AVS). Methods: To retro spectively analy ze clinical material of 97 patients with hepatocellular carcinoma with hepatic artery-portal vein shunting(HA-PVS) , of whom, 16 had upper gastrointestinal hemorrhage, 51 had middle to l arge amounts of ascites, and 53 had varices of esophagus and fundus gastricus . All the patients were treated with transcatheter arterial chemoembolization (TACE) or transcatheter arterial infusion chemotherapy (TAI). Shunts were embolized by lipidol i n 40 patients, by lipidol and spon-gia gelatinosa particle in 34 patients, and by coi l in 6 patients. The remaining 17 were treated only with TAI. Results:Twelve (12.4%) case s had hepatic artery-hepatic vein shunting (HA-HVS),while 32 (33%)cases had portal vein tumor thrombosis (PVTT). In 70(72.2%) patients, all shunts we r e completely closed successfully, in 15(15.5%) patients, the shunts were partly closed, and in 12(12.3%)patients, the shunts were not closed. In 12 patients, the shunts reopened in later therapy, their blood flow was smaller than before and they were closed after second embolization. In 17 patients, new shunts emerged. After TACE therapy,the tumors were smaller in 57(58.7%),bi gger in 24 (24.7%),and 16(16.5%) were the same in size before and after TACE. Ascit es disappeared in 29 patients, decreased in 18 patients and had no change in 4 pati ents. Five patients who had upper gastrointestinal hemorrhage pre-operation had n ew hemorrhage. Of the 47 patients who had diarrhea, 23 improved. In 67 alph a fetoprotein (AFP) positive patients, degree of AFP in 53 patients decreased. Eighty-one patients died and their Corresp onding author’s e-mail, renjieyang2007@163.commiddle survival period was 12.9 months. No serious dama ge to hepatic function due to the treatment was observed in most patients. Concl usion:Primary hepatic carcinoma with AVS increases difficulty of interv entional therapy,but as long as we take active and proper treating measure,we could ac quire satisfactory curative effect without serious syndrome. DSA can demonstrate the type, the site and the degree of AVS completely and directly, thus having import ant value in treating primary hepatic carcinoma and improving prognosis.

Background The pre-chop technique can decrease the use of ultrasound power,and thus reducing the loss of corneal endothelial cells (CECs) and injuries of other intraocular structures.Many currently developed manual pre-chop techniques restrict the wide application of the pre-chop technique because of their intrinsic disadvantages.The present study describes a manual pre-chop technique for pre-slicing the lens nucleus by using a novel reverse chopper,which has not been reported yet.Objective This study was to compare the efficacy and safety of pre-chop technique using a reverse chopper and stop-and-chop phacoemulsification technique on Ⅳ degree hard nucleus cataract.Methods A prospective clinical-controlled interventional study was performed.Thirty-two eyes of 32 cataract patients with Ⅳ degree of nucles were enrolled in People's Hospital of Hetian District from March 2015 to January 2016.The patients were randomized into the prechop group and matched stop-and-chop group according to random number table.The self-made reverse chopper-assisted pre-chop phacoemulsification surgery and stop-and-chop phaco surgery were performed on the eyes of corresponding groups,respectively.The ultrasound power,effective phaco duration,corneal endothelium loss rate,the eye number of different grade of cornea edema after operation and vision outcome were compared between the two groups.Written informed consent was obtained prior to relevant examination and surgery.Results The mean effective Phaco durations were 42.56 (39.31,45.81) seconds and 78.63 (73.85,83.40) seconds in the prechop group and stop-and-chop group,with a significant difference between them (Z =-4.937,P =0.000).The eye number with different degree of best corrected visual acuity (BCVA) was significantly different between the two groups,at postoperative day 1 and day 3 (P =0.013,0.033).The number of corneal endothelial cells (CECs) 1 month after surgery was (2 026.05 ± 154.03)/mm2 in the prechop group,and the number was (1 866.50-± 117.16)/mm2 in the stop-and-chop group,with a significant difference between them (t =3.298,P=0.003).The CECs loss rate was (13.36±2.85) ％ in the prechop group,which was lower than (25.77±3.81) ％ in the stop-and-chop group (t=-6.996,P=0.000).The eye number with different degree of corneal edema was significantly decreased in the prechop group compared with the stop-and-chop group at postoperative day 1 and day 3 (P =0.001,0.002).Conclusions Compared with the stop-and-chop phaco technique,a reverse chopper-assisted pre-chopping technique can decrease the intraoperative complication,lighten the postoperative damage of CECs and accelerate visual rehabilitation in hard nucleus cataract eyes.

Good clinical results were shown in nasopharyngeal carcinoma (NPC) patients received radiotherapy. The patients with long term life partially had radiation induced injuries that severely affect the quality of life (QOL). The study on QOL of those patients is important. Our article reviewed the effects of NPC QOL by treatment options, health education, rehabilitation therapy, traditional chinese medicine and qigong. The current measurement scale of NPC QOL in China was also discussed.