Objective:To find a good culture medium for embryo development in-vitro.Methods:D4 mouse embryo was cultured with carcinoma of kidney GRC-1 growth fluid and FD+20% fetal calf serum respectively in-vitro and the rate of zona-hatching,attachment and trophoblast outgrowth were compared respectively.Results:The rate of zona-hatching,attachment and trophoblast outgrowth with GRC-1 were 89.68%,86.51 %和84.92%,respectively.These were significantly higher than those of the control group ( P

Objective:We had analyzed the attitude of science researchers in a university to promote the development of science cause in the university. Method:We had made a prevalence survey through a questionnaire. Results:Some researchers join in science cause to acquire some profits,they often change their research orientation and they aren’t willing to cooperate with each other. Conclusion:We must initiate a kind of spirit of which advantageous to collaborate,insisting,struggling and seeking the truth of science.

For the particularity of obstetrics and gynecology disease and conventional idea of the people,clinical education of obstetrics and gynecology is very difficult.How to increase the clinical education quality of obstetrics and gynecology and how to increase the clinical'ability of medical students have become an important problem in teaching obstetrics and gynecology.In this article,we have discussed the problems and countermeasures in the teaching of obstetrics and gynecology.

Anticoagulants ; therapeutic use ; Atrial Fibrillation ; complications ; Humans ; Thromboembolism ; etiology ; prevention & control

Mr. MA Ji-xing has devoted himself into the study of acupuncture medical history for more than 70 years. As a result, a great work of Zhenjiuxue Tongshi (see text), History of Acupuncture-Moxibustion) has been completed. The author has expensively studied for history of acupuncture medicine in time and space. Base on abundant historical materials, deliberate textual research as well as strategically situated academic view, it is considered as a masterpiece of acupuncture on real significance. It is worthwhile to note that the book has a systematic and profound explanation on Bian-stone therapy, unearthed literature relics of acupuncture, the bronze figure or illustration of acupoint as well as special topics of Japan and Korea acupuncture history. Filled several gaps of the field, and explored some significant new paths of study, it laid the groundwork for the profound study and unscramble of traditional acupuncture theory as well as the investigation of the academic history, which is considered to have a profound and persistent influence. The careful sorting and profound digging of many distinguish thoughts and methods of Mr. MA Ji-xing in the study of acupuncture medical history has significant meaning in references and enlightenment of the future research on acupuncture medical history.
Acupuncture ; history ; instrumentation ; methods ; Acupuncture Therapy ; history ; instrumentation ; methods ; History, 20th Century ; History, 21st Century ; Humans

BACKGROUND: Resistin decreases insulin's action. Therefore might be a key molecule in the pathogenesis of type 2 diabetes mellitus in obese subjects.OBJECTIVE: To investigate the correlation of resistin gone expression and insulin sensitivity index (ISI) in type 2 diabetic rat models, and to explore the effect of rosiglitazone on resistin gone expression in a rat diabetic model.DESIGN: Randomized controlled animal study.SETTING: Department of Geratology, the First Affiliated Hospital of Zbengzhou University.MATERIALS: Thirty 2-month-old healthy female Wistar rats were provided by Animal Center of Tongji Medical College,Huazhong University of Science and Technology. Common diets were provided by Animal Center of Tongji Medical College, Huazhong University of Science and Technology, and the gross calorific value was 14.88 J/g (mass fraction: 0.2protein, 0.61 carbohydrate, 0.17 fat); furthermore, high-fat diets were mixed with common diets, saccharobiose, rendered lard, egg, and mild powder, and the gross calorific value was 20.083 J/g (mass fraction: 0.09 protein, 0.51 carbohydrate, 0.38fat). Resistin and β -actin were synthesized in Beijing Salbaisheng Company.METHODS: This study was performed in the First Affiliated Hospital of Zhengzhou University from October 2006 to October 2007. The experimental for animal manipulation conforms to criteria of the university's ethnic committee. Two weeks after adaptability, rats were randomly divided into normal chew group (n=8) and high-fat diet group (n=22). Rats in the high-fat diet group underwent vena caudalis injection with streptozotoein (25 mg/kg), and two days later they were continued fed with high-fat diet. For normal chew group rats were injected with sodium citrate-citrate buffer solution (1 mL/kg) and continued normal chew. After 12 weeks, 15 rats in the high-fat diet group were hyperglycemic and were randomly divided into rosiglitazone (n=8) and high-fat diet diabetic group (n=7). Rats were lavaged with rosiglitazone (2 mg/kg/d) in the rosiglitazone group and with distilled water (8 mL/kg/d) in high-fat diet diabetic group; for normal chew group rats were lavaged with the same volume of distilled water. Rats were studied for four successive weeks.MAIN OUTCOME MEASURES: Fasting serum glucose was measured with glucose oxidase method; insulin by magnetic separation enzyme linked immunosorbent assay; triglyceride and total cholesterol levels by enzymic enzymatic method; ISI was calculated as [1/(fasting serum glucose × fasting insulin)]; resistin mRNA expression in greater omental adipose tissue was examined by RT-PCR; correlation between resistin gene and ISI analyzed by Spearman correlation and multivariate stepwise regression.RESULTS: Fifteen high-fat diet rats and eight normal chew rats were included in the final analysis. Resistin gene expression in the greater omental adipose tissue (A value) was 0.27±0.031 in the high-fat diet diabetic group (0.15±0.018) in the normal chew group, and (0.20±0.024) in rosiglitazone group (P < 0.01). Spearman correlation analysis and multiple stepwise regression analysis showed that resistin gene expression was positively correlated with fasting serum glucose (r=0.271, P < 0.01), fasting insulin (r=0.283, P < 0.01), and ISI (r =0.323, P < 0.01), apparent effects of resistin gene expression on ISI was 0.081.CONCLUSION: High-fat diet and diabetes increases resistin gene expression and rosiglitazone ameliorate its expression.The expression of resistin is correlated with ISI.

Malignant tumors and the relative medical treatment of western medicine greatly affect the quality of life. This paper described the history and current situation of using acuponcture treating malignant tumor, and pointed out predominance of traditional Chinese medicine in treating tumor is treament based on syndrome differentiation and treatment according to the time of a day, which provides new ideas for acupuncture treatment of malignant tumors.

Mesenchymal stem cells (MSC) possess an innate ability for self-renewal and can differentiate into multiple cell types.MSC have been used in cell-based therapy in various disease conditions including tumors.However,as MSC therapy gained popularity among practitioners and rescarchers,there have been reports on thre adverse effects of MSC especially in the context of tumor modulation and malignant transformation.Recent studies have shown that MSC also play an important role in the resistance to various anti-cancer drugs.This review provides an overview of the functional properties of MSC in cancer drug resistance.

Intracellular calcium is one of the crucial signalings that modulate various physiological and pathological processes.Abnormal calcium signaling is closely associated with invasion and migration of neoplasms.Calcium regulates focal adhesion turnover,cytoskeletal reorganization and other processes about tumor cell movement through contacting with multiple downstream proteins,such as calpain,PYK2 and S100 protein family.Several calcium entry channels including store-operated calcium channels and transient receptor potential channels participate in the invasion and migration of tumor cells.Pathological calcium signaling may be a potential target in anti-invasion and anti-migration therapy.

Invasive fungal infection is an important cause of mortality and morbidity in preterm infants.Extremely preterm and extremely low birth weight infants are at highest risk because of the intensive and invasive nature of the care that these infants receive.How to prevent and treat the invasive fungal infection in preterm infants has become a new hot spot in the neonatologists.This review focused the progress on treatment and prevention of invasive fungal infections in preterm infants.