ObjectiveTo observe the clinical efficacy of aligned needling in treating cough in kids.MethodTotally 113 kids with cough were randomized into two groups. Fifty-sixkids in the treatment group were intervened by aligned needling; fifty-seven kids in the control group were by orally takingXiao ErKe Chuan Lingoral fluid.ResultThe total effective rate was 87.5% in the treatment group versus 66.7% in the control group, and the difference was statistically significant (P<0.05).ConclusionAligned needling and orally takingXiao Er Ke Chuan Lingoral fluid both can produce certain efficacies in treating kids’ cough, while the total effective rate of aligned needling is significantly higher.

Objective: To explore the reliability and validity of Ruminative Responses Scale in Chinese version. Methods: A total of 912 Chinese college students completed the RRS. Results: All the indexes of confirmatory analysis including ?2、RMSEA、GFI、AGFI、NNFI、CFI、IFI met the requirements of psychological assessment. The ? coefficients of the scale and subscales and test -retest reliabilities were over 0.63. There was gender difference in rumination level. Conclusion: RRS is appropriate for the assessment of rumination in Chinese colleges.

The compound Chinese materia medica is the medication pattern of the traditional Chinese medicine for the disease prevention and treatment. The single Chinese materia medica (mostly in decoction pieces) is the prescription composition of the compound Chinese materia medica. The study of the effective substance basis of Chinese materia medica should be based on the chemical compositions of the compound Chinese materia medica as an entry point considering the different status of "Monarch, Minister, Assistant, and Guide" for a certain single Chinese materia medica in the different compound Chinese materia medica while substance basis research of a certain single Chinese materia medica should be a full component analysis as well as both stable and controllable quality. Substance basis research on Chinese materia medica is one of key scientific problems of inheriting, development and innovation of Chinese materia medica.
China ; Drug Discovery ; Drugs, Chinese Herbal ; chemistry ; pharmacology ; Humans ; Materia Medica ; chemistry ; pharmacology ; Medicine, Chinese Traditional ; trends

Air Pollutants, Occupational ; analysis ; Air Pollution, Indoor ; analysis ; Chromatography, Gas ; methods ; Humans ; Organic Chemicals ; analysis ; Polycyclic Aromatic Hydrocarbons ; analysis ; Volatilization

ObjectiveTo investigate the success rate and energy requirements of ibutilide and direct current(DC)cardioversion,summarize the nursing experience.MethodsForty-six patients with persistent atrial fibrillation were enrolled and underwent DC cardioversion were selelct from May 2009 to January 2011.23 patients received pretreatment with ibutilide before DC (the ibutilide group),23 patients received non drugs before DC (the control group).The cardioversion energy started at 100 J and increased if patients could not acquire sinus rhythm.All the patients were given pertinent care before,during and posttreatment.The success rate and energy requirements of the two groups were compared.Results95.7％ of the patients were successfully cardioverted in the ibutilide group,which was significantly higher than 74.0％ of the control group.The energy required for cardioversion was ( 139.1±45.1 ) J in the ibutilide group,significantly lower than (197.8±53.3) J in the control group.Non patients terminated treatment or appeared complication because of improper nursing.ConclusionsPretreatment with ibutilide enhanced the efficacy of DC cardioversion for the patients with persistent AF at lower energy requirements.Proper nursing care can ensure the smooth process of treatment and decrease the complications.

Objective: To analyze the chemical constituents of the essential oil extracted from the seeds of Myristica fragrans Houtt.(nutmeg) in Indonesia and to provide qualities control scientific foundations.Methods: The essential oil was extracted by steam distillation and separated with GC capillary column.The relative content of every compound was determined by normalization method and the chemical compositions were evaluated by GC-MS.Results: Ninety-three chromatographic peaks were isolated and sixty-one compounds were identified,accounting for 97.62% of the total essential oil in nutmeg.Conclusion: The essential components of nutmeg in Indonesia is monoterpenoids and it's derivatives,accounting for 78.87% of the total essential oil.And phenolic compounds were the secondary composition,accounting for 16.79%.The essential oil contains safrole,which can induce genetoxicity and mutagenicity in animal,and myristicin and elemicin which can induce illusion.So,it must be careful when Myristica fragrans Houtt was used.

The prostate-specific antigen (PSA) test contributes a lot to the diagnosis and treatment of prostate cancer (PCa) and, along with imaging-guided prostate biopsy, has improved the diagnosis rate of lower-risk PCa and the accuracy of its clinical staging. However, many questions and controversies remain as to the choice of optimal biopsy strategies. Scholars differ in views about how to utilize PCa-related biomarkers to optimize the detection of initial and repeat biopsies. This review focuses on the present status of and advances in transrectal ultrasound-guided biopsy for PCa.
Humans ; Image-Guided Biopsy ; methods ; Male ; Prostate ; pathology ; Prostate-Specific Antigen ; blood ; Prostatic Neoplasms ; pathology ; Ultrasonography, Interventional ; methods

Objective To discuss the therapeutic effect of recombinant human CTLA-4Ig fusion protein (rhCTLA-4Ig) on rheumatoid arthritis (RA) patients and the change of T helper cells 17 (Th1 7 ) and regulatory T (Treg) cells.Methods Forty-eight active RA patients were randomly divided into the treatment group and the control group.Treatment group received 12 weeks of infusions of rhCTLA-4Ig ( 10 mg/kg),while the control group received 12 weeks of infusions of placebo.The changes of expression levels of Th17cell were detected by flow cytometry.The changes of expression level of FoxP3 in monocytes of the peripheral blood were examined by RT-PCR.T test and x2 test were used for statistical analysis.Results ① Eighteen of 24 cases in treatment group showed good ACR20 changes after 12 weeks,the effective rate (75％) was significantly higher than that of the control group ( 1/24,4％ ).After treatment,DAS28 score of the treatment group (3.0±0.7) was significantly lower than that of the control group(6.9±0.7) (t=-12.39,P＜0.01 ).② Compared with the Th17 monocytes of the peripheral blood from active RA patients of treatment group after therapy (0.22±0.20)％,the Th17 monocytes of peripheral blood from active RA patients of the control group (1.63±0.47)％ were higher (t=5.61,P＜0.05 ).③ Compared with the FoxP3 mRNA of the control group (0.24±0.05),that of the treatment group (0.88±0.18) was significantly higher after therapy (t=7.56,P＜0.01 ).Conclusion The clinical parameters of RA patients have improved significantly after the treatment,and the Th17 and Treg cells balance is also restored after rhCTLA-4Ig therapy.

Human FceR I a subunit extracellular domain was cloned and expressed with 2 different expressing systems and Dot blot was used to detect its biological activity to bind with IgE,providing reference for binding mechanism of human FceR I a subunit extracellular domain with IgE. It was shown that FceR I a subunit extracellular domain from pBAD/g I A expressing system could bind with IgE, but the one from PQE30 expressing system could not bind with IgE. It is suggested that FceR I a subunit extracellular domain alone is sufficient to bind with IgE without P and Y subunit. The proper space configuration and disulfide bond of FceR I a subunit is necessary for binding with IgE, but its glycosylation is unnecessary.

Objective To determine the surface expression of Fas Ag on RBL 2H3 and its function. Methods RT PCR and Western blot were used to detect the transfection and expression of Fas in RBL 2H3. Surface expression of Fas Ag was studied by immunochemistry. Apoptosis changes following treatment with anti Fas antibody were analyzed using flow cytometic analysis with annexinⅤ. Results It was successful in amplifying gene of rat Fas Ag, and a band of 32kD was detected by Western blot. The Fas Ag expression on the surface of RBL 2H3 by immunochemistry. RBL 2H3 exhibited apoptosis in response to anti Fas treatment. Conclusion Induction of rat mast cell apoptosis by activation of the Fas pathway provide the mechanism by which the number of mast cells may be regulated in the potential therapeutic strategy for anaphylactic diseases