The theory of planned behavior is used to study physician's behavior of clinical pathway compliance,on which the model of decision making mechanism for such compliance was built.This aims at exploring the relationship between the behavioral risk factors,behavioral belief and behavioral intention, and the occurrence,and recommend on further regulation of physicians'behavior and policies in hospital management.

By searching relevant data from the PubMed database, Chinese National Knowledge Infrastructure (CNKI) database and Wanfang database, a comprehensive analysis and review regarding acupuncture for cerebral ischemia reperfusion injury (CIRI) in recent 10 years were performed. The results showed that acupuncture could inhibit the inflammatory reaction, reduce oxidative stress injury, restrain brain edema formation, inhibit apoptosis, promote neural and vascular regeneration, etc. Acupuncture methods used included electroacupuncture, scalp acupuncture, eye acupuncture and "consciousness-restoring resuscitation needling", etc. The existing problem was that the intervention action of acupuncture was mainly focused on inhibiting inflammatory reaction and oxidative stress injury, and the study on apoptosis and neural and vascular regeneration was needed. It is suggested that from the aspect of multiple target points, the intervention mechanism of acupuncture for CIRI should be systemically studied in the future, which could provide new idea for clinical diagnosis and treatment on ischemic cerebrovascular diseases.
Acupuncture Therapy ; history ; Animals ; Apoptosis ; Brain Ischemia ; history ; surgery ; History, 21st Century ; Humans ; Oxidative Stress ; PubMed ; Reperfusion Injury ; etiology ; history ; physiopathology ; therapy

Objective: To study the effect of down-regulation of eukaryotic translation initiation factor 4E (EIF 4E ) induced by the siRNA expression plasmid on the proliferation and cell cycle of human breast cancer cell line MDA-MB-231. Methods: The siRNA expression plasmid pGPU6/GFP/Neo-EIF4E was constructed and then transfected into breast cancer MDA-MB-231 cells by LipofectAMINE2000. The expressions of EIF4E and cyclin D1 mRNAs and proteins were examined by RT-PCR, Western blotting and the immunocytochemistry method, respectively. The MTT method, plate colony formation assay and flow cytometry were performed to detect the proliferation ability, cell colony formation rate and cell cycle distribution of MDA-MB-231 cells transfected with siRNA expression plasmid. Results: The siRNA expression plasmid pGPU6/GFP/Neo-EIF4E was constructed successfully. The expressions of EIF4E and cyclin D1 mRNAs and proteins were all decreased markedly after transfection with pGPU6/GFP/Neo-EIF4E (P <0.05). The growth of MDA-MB-231 cells in the pGPU6/GFP/Neo-EIF4E-tranfected group was inhibited and the cell colony formation rate was statistically decreased (P<0.05) as compared with those in the blank control and the pGPU6/GFP-transfected groups. The result of flow cytometry showed that the percentage of cells in G1 phase in the pGPU6/GFP/Neo-EIF4E-tranfected group was higher than that in the blank control group (71.30%±0.47% vs 53.10%±0.43%); however, the percentage of cells in S phase was signifcantly lower (12.53%±0.13% vs 26.47%±0.38%, P <0.05). Conclusion: EIF 4E siRNA can downregulate EIF 4E gene expression in human breast cancer MDA-MB-231 cells, suppress cell proliferation to some extent, and increase the number of cells in G1 phase with a decreased number of cells in S phase. EIF 4E gene may become one of the important molecular targets to breast cancer. Copyright© 2011 by the Editorial Board of Tumor.

Adolescent ; Child ; Epidural Abscess ; diagnosis ; surgery ; Female ; Humans ; Male ; Spinal Diseases ; diagnosis ; surgery

Objective : To explore expression of related genes in breast cell line T47D induced by heregulin β1. Methods: Suppression subtractive hybridization was performed using cDNA sub traction kit to detect expression of heregulin-responsive genes in T47D cells. Results: ATP syn thase 6 was up-regulated by heregulin β1 in T47D cells at 1 and 6 hours. Conclusion: Heregulin β1 participates in the regulation of expression of both ATP synthase 6 and oxidative phosphoryla tion of T47D cells.

Objective Retrospective analysis of 56 cases of the surgical humerus neck of children that treated with abduction brace combined with manipulative reduction,and discuss the significance of abduction brace combined with manipulative reduction in treatment of fracture of the surgical humerus neck about children.Methods This study consisted of 56 children with fracture of the surgical humerus neck,among these,41 were males and 15 were females,9 were road accident and 47 were falling injury,average 7.67 years.They were all treated with abduction brace combined with manipulative reduction,after 1 month fixation,dismantled the abduction brace and did the functional exercise.Results Follow-up study was carried out in 56 cases,followed 6 months to 5 years,average 2.96 years,accoding to the standard of fracture healing,excellent result was obtained in 35 cases and good in 21 cases,the excellent-good rate was 100%.Conclusions Abduction brace combined with manipulative reduction is an effective treatment for fracture of the surgical humerus neck in children.

Objective To analyze the complications during and after the surgical treatment for long bone fractures in children with titanic elastic intramedullary nail(TEN),in order to improve the technique and potential shortcomings in these surgeries,and reduce the incidence of complications.Methods A retrospective study was done on the 182 children with long bone fractures treated with TEN from Jan.2005 to Jan.2008.A summary and analysis was made on the complications of these cases.Results All the 182 cases had the bones healed.During the operation,split in bone cortex was found in 3 cases,penetration into epiphysis by nails was found in 1 case.After operations,irritated skin and exposed nail was found in 3 cases,prolonged bone healing in 2 cases,limitation of range of motion in the knee in 1 case,bent nail in 1 case,difficulty in retracting the nail in 10 cases,shortening in 2 cases,second fracture of the healed long bone in 1 case.The results were excellent in 173 cases,good in 6 and fair in 3,and the rate of excellent and good was 98 %.Conclusions Though it has certain complications,the technique of TEN in the treatment of children's long bone fractures has more advantages over other internal fixations.Carefully performing the procedure according to the principal of TEN can decease and prevent most of these complications.

Objective To analyze the clinical presentation and maternal neonatal outcome of 30 cases with HELLP syndrome and to discuss the diagnosis, treatment and prognosis of the disease. Methods 30 cases of HELLP syndrome were collected retrospectively including 19 cases of complete HELLP syndrome and 11 partial HELLP syndrome. The blood test, clinical presentations, complications and pregnant outcomes were analyzed. Results LDH value in complete HELLP group(CHG) [(622?481)U/L] was significantly higher than that in partial HELLP group(PHG) [( 369?101) U/L, P

Age-related macular degeneration (AMD) is the mainirreversible blinding eye disease in the elderly.Its main pathogenic factors include age, genetic variation and lifestyle, but the specific pathogenesis has not been fully elucidated.As an emerging research method, proteomics technology has been gradually applied in the field of ophthalmology in recent years.A large number of studies about proteomic analysis of blood, tears, aqueous humor, vitreous humor, retina and choroid, drusen and RPE cell samples from AMD patients have been carried out to screen AMD biomarkers and explore the mechanism of AMD.These results can not only help us to make a more accurate diagnosis of AMD, but also play a guiding role in the selection of treatment targets and prognosis.

Objective To inverstigte the feasibility of video-assisted thoracoscopic surgery(VATS) be used in grass-roots hospital. Methods 46 patients were treated with video-assisted thoracoscopic surgery or video-assisted minithoracotomy surgery (VAMT) including bullectomy, lobectomy or wedge resection of lung, lung volume reduction surgery, esophageal tumor resection, clearance of hemathorax,partial pericardial,et al. Results There was no operative mortality. Complications occurred in 2 cases(4.3%). All patients were perfectly recovered by treatment. The overall follow-up was 6 months. Conclusion VATS is a safe,effective and new technique of thoracic surgery. More attentions must be payed to prevent and reduce complication in order to generalize the use of grass-roots hospital.