Sentinel lymph node (SLN) detection in endometrial cancer is still in the exploration stage. But a good many studies have confirmed the feasibility of SLN detection in patients with early endometrial cancer. Higher detection rate of SLN can be achieved by the combination of blue dye and radiocolloid. Compared with intracervical injection, more injections at different sites of the uterine corpus and hysteroscopic injection may improve SLN detection rate and para-aortic SLN detection rate. SLN detection rates are also related to some other factors such as the amount of tracers, the interval between injection and detection, and the depth of myometrium invasion. Serial-step section of SLN combined with immunohistochemistry staining with anti-cyto-keratin may improve the detection of micrometastasis (MM) in SLN. SLN procedure can detect MM which cannot be found by traditional pathological techniques. The ultrastaging will allow better stratification of patients at intermediate risk and can provide valuable informaiton for the selection of adjuvant therapy, thus decreasing the recurrent rate.

Thirty New Zealand albino rabbits of both sexes were used for the experi-ments which was (1) To observe the febrile response induced by microinjection of TNFinto the lateral ventricle; (2) To measure the PGE_2 & cAMP contents of the plasma andcerebrospinal fluid. The results showed that injection of TNF (50ng/each) into the lateralventricle did induce a definite raise of body temperature (T=0.79?0.14℃). The PGE_2concentration increased markedly in c. s. f. while that in plasma remain unchanged ascompared with the control. However cAMP lever in both, plasma and c. s. f. had nochange. These results suggested that (1) The injection of TNF into the lateral ventriclecaused a raise in PGE_2 synthesis in the brain while there was no alterations of PGE_2found in peripheral blood (2) The increase in PGE_2 didn't followed by an increase incAMP which indicated that the fever induced by TNF must have a different mechanismfrom that induced by leucocyte pyrogent (LP or In-1). PGE_2 might be involved in thefever inducing mechanism but these results don't support the hypothersis that the raiseof PGE_2 level may increase the cAMP content which in turn reduced the fever.

Objective:To report one case of hilar hepatic tumor treated by ultrasound-guided percutaneous puncture 125 I radioactive seed implantation,and to review the associated literatures.Methods:One patient’s CT and ultrasonic examinations were taken,and the plan of TPS was taken.The patient was treated by ultrasound-guided percutaneous puncture 125 I radioactive seed implantation according to the plan of TPS.The needles were parallel, and the distance between the seeds were 1.0 to 1.5 cm. Results:The operation was successfully completed.No postoperative complications were encountered during 1 month follow up.The volume of tumor was obviously reduced 1 month after operation detected by ultrasound and CT. The local control effect of tumor was good.Conclusion:Ultrasound-guided percutaneous puncture 125 I radioactive seed implantation is a safe and effective method in treating hilar hepatic tumor.

This article outlined the necessity of integrated information literacy education,ex-pounded the connotation of information literacy and integrated education and analyzed the situation of integrated information literacy education in China's medical colleges. In the end,the measures of inte-grated information literacy education were promoted from the following perspectives:improving the level of information literacy of professional teachers,designing the curriculum system of information literacy,integrating with medical professional courses,and establishing a network learning platform with professional education and information literacy education.

Objective Primary myocardial cell culture , as an important technique for in vitro experiment , provides an essen-tial basis for the establishment of models of cardiovascular diseases .This study aimed to investigate the influence of digestion time on primary myocardial cell culture and to find some simple primary culture methods for obtaining myocardial cells with high survival rate , purity, and activity. Methods We used only one collagenase for repeated digestion of myocardial tissue for 8 and 10 minutes, re-spectively , and obtained high-purity cardiomyocytes by differential adhesion and Brdu inhibition .We observed the morphological chan-ges of the cardiomyocytes under the optical microscope , measured their vitality according to the beating frequency in different regions , and counted the living cells after typan blue staining. Finally, we established a model of myocyte hypertrophy for verification of cell activity and compared the cell surface area between the experimental ( angiotensinⅡ) and control ( 10%FBS DMEM [H +] medium ) groups. Results After cultured for 48 hours, the primary cardimyocytes in the 10 min group mostly extended pseudopodia , in-terwoven into a network and gradually forming cell clusters , with obviously powerful beating and contraction .After isolation and purifi-cation, the average survival rate of the cells was >90%, significantly higher in the 10 min group than in the 8 min ([95.4 ±0.8]%vs [93.0 ±0.8]%, P<0.05).The positive expression rate of cardiac α-actin was >95%in both of the groups.After cultured with angiotensinⅡfor 48 hours, the 10 min group showed a significantly increased level of atrial natriuretic peptide (ANP) (20.8 ±0.67 vs 15.8 ±0.48, P<0.05) and cell surface area ([34.77 ±8.43] μm2 vs [14.11 ±4.29] μm2, P<0.05) as compared with the 8 min group. Conclusion Primary culture of cardimyocytes with 8 min digestion is simple and time-saving, and what is most important , can obtain high-quality cardiomyocytes .

Objectives:To observe the therapeutic effect of Cuochuang Misture on acne vulgaris and study the mechanism by external bacteria repressing experiment.Methods:34 cases of acne of syndrome of dampness and heat in lung and stomach were treated with Cuochuang Misture and 28 cases with Dangguikushen Pill as controls,and their curative effect were compared;Different concentration of Cuochuang Misture were used to inhibit Propionibacterium acnes(P.ance)and Malassezia furfur(M.furfur).Results:The clinical total effective rates were 94.12%in the treatment group,and 89.29%in the control group.The scores of skin lesions and syndromes reduced obviously between two groups in the end of the treatment(P

Objective To study the effect of seven sevoflurane or propofol combined with remifentanil anesthesia on patients undergoing laparoscopic surgery.Methods In Taizhou Tumor Hospital from January 2015 to December 2016 were 100 cases of patients with laparoscopic surgery as the research object in the course of the study, were randomly divided into control group and experimental group two were 50 cases each.Patients in the control group were treated with propofol and remifentanil anesthesia.The experimental group was given seven sevoflurane and remifentanil anesthesia.Comparative analysis of the experimental group and the control group of patients with anesthesia effect.Results After the corresponding anesthesia, patients in the experimental group extubation time, recovery time was significantly better than the control group, with statistical difference(P<0.05).In the experimental group, 15 minutes after extubation, the Steward recovery score was(5.04±0.65), while the control group score was(4.12±0.54).Can be obtained, the quality of patients' recovery was significantly higher than that of the control group, with statistical difference(P<0.05).The cognitive function scores of the experimental group were significantly higher than those of the control group after extubation(2 h), and the difference was statistically significant(P<0.05).Conclusion Compared with propofol remifentanil anesthesia, seven sevoflurane remifentanil anesthesia effect is good, can improve the patient's recovery quality, to help with the recovery of cognitive function, with further clinical promotion and application significance.

Objective To study the effect of different doses of dexmedetomidine combined with propofol and remifentanil in patients undergoing abdominal surgery. Methods 200 cases of abdominal operation in our hospital from January 2015 to August 2016 were selected as the subjects, and were randomly divided into the experimental group and the control group, with 100 patients in each group. Patients in the control group received 0.4μg/kg, dexmedetomidine hydrochloride injection combined with propofol and remifentanil anesthesia, and the experimental group received 0.8μg/kg, Dexmedetomidine Hydrochloride Injection anesthesia. The adverse reactions, extubation time and recovery time of the experimental group and the control group were compared and analyzed. Results After the corresponding dose of dexmedetomidine, the patients in the experimental group were 6 cases of nausea and vomiting, 7 patients with chills, 13 cases of adverse reactions, the total incidence was 13%. In the control group, shivering occurred in 23 cases, nausea and vomiting occurred in 12 patients, and the number of adverse reactions in 35 cases, the incidence of adverse reactions was 35%. The probability of adverse reactions in the control group was significantly higher than that in the control group, with statistical difference (P<0.05). The recovery time and extubation time of the experimental group were significantly lower than those of the control group, with statistical difference (P<0.05). Conclusion 0.8μg / kg dexmedetomidine hydrochloride Iinjection combined with propofol and remifentanil anesthesia in abdominal surgery is better anesthesia effect, can significantly shorten the time of patients with tube drawing, high safety, reduce the pain and risk of surgery patients has clinical significance in the further promotion and application.

The preventive maintenance of medical instruments is very important in field hospital. Because of effective preventive maintenance,the ultrasonic instrument ran normally and efficiently in field hospital. The method of preventive maintenance are introduced,including quakeproof,power supply,hot & moist resistance,dustproof,antijam,probe maintenance,etc. These maintenance methods can provide reference for diversified military affairs.

Influence on diagnosis equipment in field hospital for earthquake relief are stated such as weather, voltage, noise and interference. It is necessary to maintain diagnosis equipment in field condition.