Objective: The paper inquires into a new external temporary cardiac pacing method. Method: The research uses a microcomputer-based multi-functional external temporary cardiac-pacing device made by the authors for sending pacing signals. The pacing mode and pacing rate of the device can be adjustable. The pacing pulse width and pacing current of the device can also be adjustable. It inserts a syringe necdle-like negative pacing pole into the chest muscle in the prior-cardiac area of a male adult mongrel dog; it pastes a positive pacing pole on the back area near heart of the dog. It takes intravenous injection isoptin 5mg into the male mongrel dog. After 5 minutes, the heartbeat of the dog suddenly stops; it does pacing experiment for the dog at this time and observes the results of the pacing experiment. Results: After temporary cardiac pacing, the heartbeat of the dog returns, pacing ECG of the dog appears, the pacing for the dog is active. Conclusions: It is a new and benefit external temporary cardiac pacing method.

Bacterial translocation is a major contributor to sepsis and multisystem organ failure.This paper reviews the studies in recent years.We will briefly introduce the advance in bacterial translocation,and expound its pathogenesis,prognosis,diagnosis,therapy,as well as significance and prospects.

Objective To explore the feasibility of two-port laparoscopic drainage in the treatment of liver abscess. Methods A total of 18 patients with bacterial hepatic abscess underwent twoport laparoscopic drainage.Two incisions were made at the superior border of the umbilicus and 4～5 cm below the right costal margin near the anterior axillary line,respectively.The abscess was cut through in "┼" or "╫" shape.The pus cavity was debrided bluntly,and a double-lumen drainage tube was placed.Results The laparoscopic drainage was performed successfully in all the 18 patients.The operative duration was 30～75 min(mean,45 min).A bile leakage for 80～100 ml per day occurred in 1 patient.The drainage tube was removed on the 12th postoperative day and the patient was discharged from hospital on the 14th postoperative day.Of the 18 patients,the hospital stay after operation was 7～14 d(mean,8.5 d).All the patients were followed for 5～12 months(mean,9 months) and no residual abscess or recurrence was found. Conclusions Two-port laparoscopic drainage for liver abscess is an ideal approach with advantages of simple manipulation,slight contamination inside abdominal cavity,little trauma,low complication rates,and rapid recovery.

Objective To investigate the types of commonly used anti-hypertensive drugs and hypoglycemic drugs and treatment schemes to provide a basis for rationally treating hypertension complicating type 2 diabetes mellitus(T2DM).Methods The medication situation in 191 patients with hypertension complicating T2DM in our hospital were analyzed and investigated.The use of anti-hypertensive drugs and hypoglycemic drugs and treatment scheme were statistically analyzed.Results In 191 patients with hypertension complicating T2DM,the single drug use in the anti-hypertensive scheme accounted for 9.42 ％,two-drug combined use accounted for 64.91％,three-drug combined use for 17.8 ％ and four-drug combined use for 7.85 ％.The medication of ARB+CCB had the highest use frequency;the anti-hypertensive total effective rate was 96.86 ％.In the hypoglycemic schemes,the insulin use accounted for 30.37％,and the use frequency of metformin+ gliclazide was highest,the hypoglycemic total effective rate was 98.43％.Conclusion The medication for the patients suffering from hypertension complicating T2DM is relatively reasonable,and worth of being popularized and applied in clinical practice.

One of the important therapeutic measures in the acute stage of cerebral hemorrhage is to treat cerebral edema and lower intracranial pressure. Mannitol, an osmotic dehydrant, is most widely used in clinical practice. Its major effects include the reduction of intracranial pressure and cerebral edema and prevention of cerebral herniation. Besides, mannitol scavenges oxygen free radicals and protects brain cells. So it has extremely important significance in reducing the mortality of intracerebral hemorrhage.

BACKGROUND:Transient receptor potential protein channel is distributed in many kinds of tissue and cells in human body, and takes part in many physiologic functions and pathological changes of human body. However, up to now, transient receptor potential melastatin 8 (TRPM8) has not been reported in human cartilage.
OBJECTIVE:To study the expression of TRPM8, a subfamily member of transient receptor potential gene, in human knee joint cartilage.
METHODS:Fifteen samples were selected from patients after amputatioin due to severe trauma. Total protein was extracted from 300 mg cartilage tissue of every patient. To detect the distribution of TRPM8 in human
cartilage, cartilage sections were col ected in the same region. The expression and distribution of TRPM8 in human cartilage was detected by western blot analysis and immunofluorescence respectively.
RESULTS AND CONCLUSION:Western blot analysis showed that specific TRPM8 band was visible at molecular weight of 130 000, and as the age increased, TRPM8 expression in human cartilage was gradual y decreased. Immunofluorescence staining results showed that TRPM8 was evenly located in the cellmembrane of chondrocytes. TRPM8 is expressed in the human knee cartilage, specifical y in member of chondrocytes.

Objective To study expression of matrix metalloproteinase‐9(MMP‐9) protein and its mRNA in breast cancer and e‐valuate its significance in the occurrence ,development and metastasis of breast cancer .Methods The protein expression of MMP‐9 breast cancer were detected by using immunohistochemistry and the expression of mRNA were detected by reverse transcription polymerase chain reaction(RT‐PCR)Results The positive rate of MMP‐9 protein expression in 56 cases of breast cancer was 69 .6% (39/56) ,while in benign breast diseases was 20% (6/30) ,which were significantly different(P<0 .05) .The levels of MMP‐9 mRNA were significantly higher in the patients with breast cancer than those in benign breast diseases(P<0 .05) ,which were 0 .914 2 ± 0 .108 1 and 0 .379 4 ± 0 .0428 respectively .Conclusion The MMP‐9 protein and mRNA expression in human breast cancers are positively correlated with the stage and lymph node metastasis .Expression of MMP‐9 could be used as an indicator for the ability of invasion and metastasis of breast cancer .

Objective To investigate the residues of antibiotics such as quinolones in fishes from Guangzhou aquatic products market.Methods The residues of three kinds of quinolones(NFLX,CPFX and ENFX) in four kinds of fishes collected from five agriculture products markets in Guangzhou were determined by RP-HPLC.Results NFLX was detected in all samples,ranged from 2.19-192.8 ng/g with the maximum concentration of 192.8 ng/g,the detection rate of CPFX was 40% with the average concentration of 4.34 ng/g,ranged form 0-25.36 ng/g,the detection rate of ENFX was 45% with the average concentration of 6.83 ng/g.Three kinds of quinolones were found with high detection rate in Micropterus salmoides and Anguilla japonica,the average detection rates of NFLX,CPFX and ENFX in muscle tissues of Micropterus salmoides were 100%,40% and 100% respectively,that in Anguilla japonica were 100%,40% and 80% respectively.Low rate of ENFX was found in Monopterus albus and Ctenopharyngodon idellus muscle tissues,the average detection rates were 20% and 10%.Higher pharmaceuticals concentrations were detected in the liver than those in the muscle tissue.Conclusion The residues of quinolones in the muscle tissue of fish sold in Guangzhou is considered eligible based on the related standards.

Objective To investigate the effect of non-hazard treatment of feces on control of intestinal infectious diseases and parasitosis. Methods During Nov, 2003-May, 2004, we selected 6 counties as observational points by the character of geographical position and the types of non-hazard treatment in Hunan province. The number of fecal coliform and the sedimentation rate of parasite ova were measured, the methods of questionnaire and retrospective study were used and the prevalance rate of intestinal infectious diseases, the incident rate of infectious diarrhea and infectious rate of parasite were analyzed. Results Among three types of non-hazard treatment, the eligibility rates of fecal coliform were 95.0%, 93.5%and 91.7% respectively,and the sedimentation rates of parasite ova were 90.0%, 90.3% and 87.5% respectively. Compared with the villages where the latrines had not been improved, the infectious rates of parasite decreased by 67.0%,the prevalance rate of intestinal infectious diseases decreased by 58.3%, and the incidence rates of infectious diarrhea decreased by 56.3% in villages where the latrines had been improved. Conclusion Non-hazard treatment of feces will play an important role in preventing of intestinal infectious diseases, infectious diarrhea and parasitosis.