Objective To establishment the model of angiotensin Ⅱ (AngⅡ) inducinged cardiomyocyte hypertrophy in H9c2 myocardial cells, and observinge the the changes of ubiquitin binding protein leveljunction protein ubiquitination level. Methods Myocardial cell isolated and subcultured H9c2 were cultured in vitro andmyocardial cell, they were divided into three groups in different AngⅡ concentration (10-8, 10-7, 10-6 mol/L), with in different hours (0, 6, 12, 24, 48, 72 h), and there is also a control group; measured the total protein contentlevel were measured useby BCA method , measured the number of cardiomyocytes in the same area were measured, and the relative surface area of myocardial cells were calculated. The expression of ubiquitin binding protein cardiomyocyte hypertrophy was time- and concentration-dependently induced by AngⅡ, wheremanifested, as total cellular protein content,relative cell surface area and the expression of ubiquitin binding proteinubiquitinated desmin significantly increasing compared with the control group (P < 0.05), which were most significant in 10-7 mol/L AngⅡtreated group for 48 h. Conclusions Cardiomyocyte hypertrophy model induced by 10-7 mol/L AngⅡ for 48 h , was successfully established , where H9c2 cardiomyocyte hypertrophy was induced by 10-7 mol/L AngⅡ in 48 h, and ubiquitin binding proteinubiquitinated desmin significantly increased in cardiomyocyte hypertrophy.

OBJECTIVE:To investigate the "drug use for pregnant women" in drug package inserts and to provide reference in standarding the drug package inserts.METHODS:375 package inserts of common drugs collected in our hospital were analyzed statistically in terms of the item of "drug use for pregnant women".RESULTS:Among the 375 drug package insert,134 stated "forbidden or contraindicated for pregnant women";51 stated "to be used with caution for pregnant women",40 had indefinite explanation about the drug use for pregnant women,and 23 had no explanation for drug use for pregnant women.CONCLUSIONS:It is advisable for the related department in our country tighten control on the safety of drug package inserts by organizing experts to conduct a regular post-marketing reevaluation on the drugs,urging drug manufactures to timely update the drug package inserts,setting and improving the standard format of drug package inserts to ensure the safety of clinical drug use.

Objective To explore the effect of MPCNL in the treatment of complex proximal ureteral calculi.Methods 90 cases were involved in treatment of complex proximal ureteral calculi in our hospital.MPCNL group were 45 cases and U RL group surgery were 45 cases.Results The operation time,bleeding,single surgery success rate,stone-free rate after surgery success for URL and MPCNL were(34.8 ± 10.5) minutes and ( 112.5 ± 12.4) minutes,(12.5 ± 4.6)ml and (51.3 ±3.7)ml,71.1％ and 95.6％,91.1％ and 97.8％ respectively(P ＜ 0.05).Conclusions The operntive time of URL is shorter,blood loss of URL is less than those of MPCNL,but the success rate of surgery and stone-free rate of URL is not higher than those of MPCNL.Both treatment methods are safe and reliable without serious complications.

West syndrome is one of the most common refractory epileptic syndromes with onsets mostly in infancy.The prognosis is generally poor.The morbidity rate reported in the literature is 0.2‰ to 0.5‰.Hormonal therapies(Adrenocorticotropic hormone and glucocorticosteroid)are recommended as preferred treatment options for West syndrome.It can effectively control spasms, ameliorate recognition impairment, and improve developmental outcomes.The incidence of side effects after hormonal therapy are high, and irritation, hypertension and infection are frequently reported.In this paper, advances in therapeutic mechanism, usage and dosage, short-term effectiveness, long-term effectiveness, prognosis and adverse effects of hormonal therapies are reviewed.

Objective:To design real-time transmission system for pre-hospital emergency electrocardiogram(ECG) so as to provide specialty and accurate diagnostic analysis about ECG before patient achieved at hospital and to shorten the waiting time for diagnosis and treatment after they achieved at hospital.Methods: 4G network and virtual private dial-up network(VPDN) were used to real-time transmit ECG data of patient from ambulance to hospital so as to doctors of ECG department and emergency room can analyze the detecting reporter, choose plan of emergency guidance and prepare operation and treatment for patientsaccording to the received ECG data in time.Results: The situations of emergency patients have been grasped and been primarily confirmed before they achieved hospital, therefore, the doctors could made relative preparation for fast and specially treating patients after they once achieved hospital.Conclusion: The real-time transmission system of pre-hospital emergency ECG can remedy the shortage of technical strength of pre-hospital emergency team, and shorten the treating time of emergency patients, and increase the success rate of rescue.

The feasibility and mechanism study on intravenously injected bone marrow mesenchymal stem cells (MSCs) improving bladder function after spinal cord injury (SCI) were discussed by review the biological characteristics of MSCs, the changes and mechanism of the lower urinary tracts after SCI, and the study on the MSCs promoting recovery of bladder function.

Objective To study the effect of capsule deficient strains CAP64 on apoptosis of mice microglia cell line (N9) cell in vitro . Methods N9 cells were co cultured with CAP64, the flow cytometry was employed to detect the N9 cellular apoptosis on different phase. Results The average apoptotic index of N9 after cultured for 2、4、8、16、24 hours with CAP64 were 9 33%、12 93%、15 37%、16 30% and 20 5%, respectively. There were significant differences between experimental group and control group. Conclusion Capsule deficient strains CAP64 can induce microglia cell line (N9) apoptosis

Objective To study the metastasis rule and influence factors of cervical central group (Ⅵ region) lymph node in patients with papillary thyroid microcarcinoma.Methods The clinical data of 195 patients with papillary thyroid microcarcinoma were analyzed retrospectively.All patients received bilateral thyroidectomy and bilateral cervical central group lymph node dissection.Results All patients died without surgery and hospitalization,the incidence of postoperative complications was 2.1％ (4/195).The total incidence of cervical central group lymph node metastasis was 35.4％ (69/195).Factors relating to cervical central group lymph node metastasis rate were:age (OR =0.924),gender (OR =0.213),the number of foci (OR =2.208) and the sum of the tumor diameters (OR =2.106).Conclusions Patients with papillary thyroid microcarcinoma may suffer from lymph node metastasis of the cervical central group,and lymph node metastasis rate increases significantly in young or male patients,and when the lesions are multifocal or the sum of the tumor diameter ＞ 0.5 cm.

0.05). The vanadium and titanium contents in surface water were higher than those in ground water (P

[Objective] To explore the effect of B-Twin intervertebral cage for degenerative lumbar instability with posterior mini-incision.[Methods]Thirteen patients(13 disc-spaces)treated with B-Twin intervertebral cage in combination with autogenous morselized bone were enrolled in this study.Among the patients,11 had degenerative instability in L4、5 and 2 in L5S1.Patients were graded postoperatively with JOA scoring system.[Results]The lower back pain and leg pain were relieved significantly in all patients after operation.All were followed-up for an average of 16.2 months,and the JOA scores inproved from 4.2 preoperatively to 14.4 at the final follow-up.The good to excelleat rate was 92.3%.The bony fusions presented in an average of 19.8 weeks postoperatively by CT scan.Neither cage loosening nor neurological injury was found.[Conclusion]B-Twin intervertebral cage combined with autogenous morselized bone insertion for lumbar degenerative instability by posterior mini-incision operation is a good technique,which could achieve satisfactory clinical results with less injury.