Objective To discuss the clinical value of aspartate aminotransferase mitochondrial isoenzyme(m‐AST ) in serum to alcoholic liver diseases .Methods 61 cases of patients with alcoholic liver diseases and 60 cases of patients with nonalcoholic fatty liver disease in our hospital from Jan 2015 to Dec 2015 were selected as research subjects ,and 60 cases healthy volunteers in the same period were selected as control group ,and then their venous blood samples were collected .ELISA was used to detect the levels of aspartate aminotransferase mitochondrial isoenzyme (m‐AST ) ,aspartate amino transferase (AST ) ,alanine aminotransferase (ALT) and gamma glutamyltransferase (GGT) in serum ,the differences between patients and volunteers were compared .Results The levels of m‐AST ,AST ,ALT and GGT in group A and group B were significantly higher than those in healthy control group (P<0 .05);After 3 months treatment ,the difference of m‐AST ,AST ,ALT and GGT in patients with fatty liver ,viral hepatitis and liver cirrhosis was significant(P<0 .05);The positive rate of m‐AST were significantly higher than other index(P<0 .05) .Conclu‐sion The detection of m‐AST is significance to diagnosis and treatment for alcoholic liver diseases .

Hospital culture and ethics in voluntary dependence quit institution relate to the implement of its function.Medical Ethics which base on the modern bioethics and social ethics is one of the important foundations of cultural construction in voluntary dependence quit institution.It also plays an important role in the fight of drug.

Objective To investigate the effect of Sufentainil on myocardial ischemia-reperfusion injury and caspase-3 to rat heart. Methods Ninety Wistar rats weighing 200-300g were randomly divided into 3 groups(n=30):sham group(group S), ischemia-reperfusion group (group I/R)and Sufentainil group (group SF). Each group was divided into 3 subgroups according to the time phases at 30, 60 and 120min(n=10).In group I/R and group S the animals were received normal saline intravenously. In group SF sufentainil 5μg/kg NS 20ml was given. After 24 h, all animals were anesthetized with intraperitioneal 20% urethane 1 mg/kg, intubated and mechanically ventilated. The chest was opened and heart exposed via thoracotomy.In group S , left artery anterior descending artery(LAD) was not ocluded. While in I/R and SF group myocardial ischemia was introduced by clamping LAD for40 min. The animals were killed at 30, 60 and 120 min of reperfusion respectively(n=10each). Cardiac tropoin I(cTnI), expression of caspase-3 and apoptosis of cell in heart tissues were measured at the same time.Ultrastructure of myocardium taking the from apex was examined by electron microscopy at the end of 120 min reperfusion. Results Compared with group S, the concentration of cTnI was increased in the other 2 groups(P<0.05).cTnI was significantly lower in group SF than in group I/R(P<0.05). Expression of caspase-3 and apoptosis index induced by myocardial reperfusion were attenuatated in group SF(P<0.05). The damage of myocardial ultrastructure caused by myocardial I/R were also significantly improved in group SF. Conclusion Sufentainil may relieve the heart cellular apoptosis by decreasing caspase-3expressing.

The brain computer interface (BCI) can be used to control external devices directly through electroencephalogram (EEG) information. A multi-linear principal component analysis (MPCA) framework was used for the limitations of tensor form of multichannel EEG signals processing based on traditional principal component analysis (PCA) and two-dimensional principal component analysis (2DPCA). Based on MPCA, we used the projection of tensor-matrix to achieve the goal of dimensionality reduction and features exaction. Then we used the Fisher linear classifier to classify the features. Furthermore, we used this novel method on the BCI competition II dataset 4 and BCI competition N dataset 3 in the experiment. The second-order tensor representation of time-space EEG data and the third-order tensor representation of time-space-frequency BEG data were used. The best results that were superior to those from other dimensionality reduction methods were obtained by much debugging on parameter P and testQ. For two-order tensor, the highest accuracy rates could be achieved as 81.0% and 40.1%, and for three-order tensor, the highest accuracy rates were 76.0% and 43.5%, respectively.
Algorithms ; Brain-Computer Interfaces ; Electroencephalography ; Humans ; Principal Component Analysis

Objective To assess voice parametric characteristics of normal children in different age?sex?and phonation conditions. Methods Voice data of 240 children were collected and analyzed with Dr. Speech software. Acoustic signal data were measured in three phonation conditions: the lowest modal voice, natural voice, the highest modal voice. Results Perturbations and normalized noise energy from the highest value to the lowest one by one were that of the lowest modal voice, natural voice, the highest modal voice. The sequences of standard deviation of fundamental frequency were that of the highest modal voice, the lowest modal voice, natural voice. Conclusion The voice quality of normal children had distinctive characteristics in different phonation conditions.

Objective To determine the sites of action of propofol and isoflurane on somatosensory pathway using median nerve somatosensory evoked potential(MnSSEP) .Methods Twenty-six ASA I - II patients aged 20-50 yrs were randomly divided into two groups: I propofol group( n = 13) and H isoflurane group( n = 13) .In propofol group patients received propofol infusion at a rate of 10 mg?kg-1?h-1 .Oxygen was administered via mask and respiration was assisted or controlled to maintain SpO2 at 96%-100% and PETCO2 at 35-45 mm Hg. The propofol infusion was continued until the patient failed to respond to verbal command. Six minutes later if the patient was still breathing spontaneously, the rate of propofol infusion was increased to 20-40 mg?kg-1?h-1 . In isoflurane group anesthesia was induced with propofol 2 mg ? kg-1 and intubation was facilitated by succinylcholine and anesthesia was maintained with isoflurane inhalation and intermittent iv boluses of vecuronium. The end-tidal isoflurane concentration was maintained at 0.5,1.0 and 1.5 MAC. Each concentration was maintained for 15 minutes. MAP, HR, SpO2,PCTCO2, T0(naso-pharyngeal) and BIS were continuously monitored. MnSSEP( Viking IV D type) was measured and recorded before induction of anesthesia(baseline) and in propofol group when patients became unconscious and apneic; in isoflurane group when end-tidal isoflurane reached 0.5,1.0 and 1.5 MAC.Results In group I there was no change in both latencies and amplitudes of N9 and N13'.With increasing infusion rate, propofol gradually prolonged the latencies and decreased the amplitudes of N60, P45, N35, N20 and P25 waves. In isoflurane group there was no change in the latencies and amplitudes of N9. There was no change in the latencies of N13', but the amplitudes decreased at 1.0 and 1.5 MAC. With increasing concentration, isoflurane gradually prolonged the latencies and decreased the amplitudes of P45, N60, N35, N20 and P25 waves. At 1.5 MAC the inter-peak latencies between N13'-N20 and N13'-P25 were prolonged. Conclusions The sites of action of different infusion rates of propofol and different concentrations of isoflurane are different on somatosensory pathway. The higher the doses, more widespread are the sites of action.

glycerol,and the best penetrable concentrations for it were 0.5%,0.5% and 0.2%,respectively.No obvious penetration efficacy was noted for glycerol.CONCLUSIONS: 4 kinds of penetrations enhances can increase the percutaneous absorption of DH under the certain concentration.

OBJECTIVE:To establish the quality standard of Jinwu jiangu capsule. METHODS:Microscopy was used to identi-fy Panax notoginseng;TLC was used to identify Paeonia lactiflora and Sinomenii Caulis;HPLC was used to determine the content of paeoniflorin. The column was ZORBAX SB-C18 with mobile phase of acetonitrile-0.1% phosphoric acid(13∶87,V/V)at a flow rate of 1.0 ml/min,detection wavelength was 230 nm,column temperature was 30 ℃ and injection volume was 10 μl. RESULTS:Microscopy showed P. notoginseng had obvious features;P. lactiflora and Sinomenii Caulis in preparation were well-separated with clear spots. The linear range of paeoniflorin was 0.014-1.412 μg/μl(r=0.999 2);RSDs of precision,stability and reproducibility tests were lower than 2%;recovery was 95.73%-99.48%(RSD=1.54%,n=6). CONCLUSIONS:The method is simple,accurate and reproducible,and can be used for the quality control of Jinwu jiangu capsule.

Objective To explore the effects of TMPRSS2 promoted suicide gene CD-5-FC combined with phytochemicals on the proliferation and apoptosis of prostate cancer (PCa) specific cell-line 22RV1.Methods From March 2016 to October 2016 TMPRSS2-VISA-CD/UPRT'vector was used to deliver pro-drug 5-FC (5-Fluorocytosine) in to PCa specific cell-line 22RV1.Transfection effect was verified by Western-blotting.5-FC,5-FU (5-Fluorouracil) and four phytochemicals epigallocatechin gallate (ECGC),Genistein,Daidzein,and Equol were selected in this study.MTS assay was performed to select dosages,which can kill 10％-40％ of 22RV1 cells,from their pre-set drug concentrations,respectively.According to their select concentrations,either 5-FC plasmid or 5-FU in combination with one of four phytochemicals were added in 22RV1 cells in orders,and then cultured them together.Besides,these four phytochemicals were added in 22RV1 cells individually regarding their selecting concentrations and cultured at the same time.Cell viability was detected by MTS assay on 24hrs,48hrs and 72hrs.Comparing the cell killing rates between the combination groups and each single drug groups on cell-line 22RV1.Results TMPRSS2-VISA-CD/UPRT'plasmid with 5-FC was transfected on PCa specific cell-line 22RV1 successfully.The most obvious transfection occurred on 48hrs.The selected concentration of the pro-drug 5-FC were 83.32 μmol/L and 833.20 μmol/L,5-FU were 76.87 mol/L and 768.70 μmol/L.The concentrations of four phytochemical agents were 2.00 μmol/L,10.00 μmol/L and 20.00 μmol/L.In term of the cell killing numbers,results showed that the combination treatment of 83.32 μmol/L 5-FC/ 76.87μmol/L 5-FU and 10.00 μmol/L/20.00 μmol/L four phytochemicals compared with 10.00 μmol/L/20.00 μmol/L single treatment of four phytochemicals,respectively,there was no significant difference (P ＞0.05),while the combination treatment of 833.20 μmol/L 5-FC/768.70 μ mol/L 5-FU and 2.00 μmol/L/10.00 μmol/L four phytochemicals compared with 2.00 μ mol/L/10.00 μmol/L single treatment of four phytochemicals,respectively,there was a significant difference (P ＜ 0.05).In addition,this study proved that 83.32 μmol/L 5-FC and 76.87 μmol/L 5-FU,833.20 μmol/L 5-FC and 768.70 μmol/L 5-FU reached the same cell killing effect,and there was no statistical difference (P ＞ 0.05).Thus the suicide gene transfection was successful,and its function was the same as the effect of cytotoxic drugs.Conclusions This study has proved that this plasmid with 5-FC suicide gene system can be successfully and efficiently transfected into PCa cells 22RV1.As the pro-drug of 5-FU,5-FC got similar treatment effect with 5-FU,and inhibited cell proliferation.There was no synergistic reaction of enhancing cell apoptosis after combined phytochemical drugs EGCG,Genistein,Daidzen,Equol with suicide gene therapy on 22RV1 cell-line.

Objective Ryanodine receptor (RyR) is one of the Ca2+ release channels on the intracellular Ca2+ stores. RyR induced-Ca2+ release is activated by the voltage-dependent Ca2+ entry, that is, calcium-induced calcium release (CICR). Intracellular free Ca2+ concentration (ECa2+]i) plays a key role on cochlear function. Our study is to investigate the differential expression of RyR in the developing rat cochlea, and to analyze the relationship between the expression of RyR and auditory functional development. Methods Immature SD rats, which were 1 day (P1), 5 days (P5), 10 days (P10), 14 days (P14), 28 days (P28) after parturition, and adutl rats(5 rats for each age) were included in the study. Frozen cochlea sectioning and immunofluorescence were applied to observe the differential expression of RyR. Results The RyR expression in the Corti's organ increased during the cochlear development. It's not significant that the stain was observed on the hair cells and supporting cells in the Corti's organ of P1 and P5 rats. The appearance of the Corti's organ of P10 rats trended to maturity. In P14 rats the RyR expression on hair cells located in the synaptic area against the afferent or efferent nerve, and the strain on supporting cells was extensive. There was little different between the strain on cochlea of P14 and P28 rats. In postmature rat spiral ganglion neurons (SGN), the RyR expression verged gradually from extensive whole cell soma to the synaptic area near to the plasma membrane. Conclusion The RyR expression peaked the 14th day after parturition, which was close to that in the mature cochlea. The time course of the RyR expression during the cochlear development was coincident with that of the auditory functional development. The RyR expression on both hair cells and SGNs located in the synaptic area near to the plasmolemma, implying that RyR induced-CICR was related to the auditory functions such as neurotransmission. Extensive RyR expression in the soma of SGNs at the early stage possibly involved in apoptosis of SGNs during neuron development.