Inducible costimulatory molecule (ICOS) is one of the costimulatory molecules CD28 superfamily and is upregulated on the surface of T cells following T cell activation.ICOS initiates costimulatory signals after binds with its ligand (ICOSL) and ICOS/ICOSL promote activation of T cells proliferation,differentiation and cytokine production.Clinical and laboratory researches documented that ICOS/ICOSL plays significant roles in various autoimmune diseases,similarly,it plays critical effects on the outcome and evolution of autoimmune uveitis (AIU) and Behcet disease.To further study the influence and mechanism of ICOS/ICOSL pathway on AIU is of helpful for finding out a new therapeutic approach of AIU.This paper reviewed the basic concept of ICOS,the relationship between ICOS/ICOSL pathway and effector cell,the outlook of ICOS/ICOSL in the treatment of AIU.

Thirty-eight cases of allergic rhinitis has been treated by puncturing the points of Yintang (Ex-HN 3), Yingxiang (LI 20), Feishu (BL 13), Fengchi (GB 20), Shangxing (GV 23), Hegu (LI 4) and so on, combined with massaging the head, face and trunk. The result showed that cure occurred in 16cases, marked effectiveness in 18 cases and ineffectiveness in 4 cases.

ObjectiveTo investigate the effects of HIF-1α,on mitochondrial function of cardiomyocytes stimulated by lipopolysaccharide (LPS).MethodsCultured neonatal cardiomyocytes were randomly (random number) distributed to four groups (n =5).Control group:the neonatal cardiomyocytes were cultured in normal condition without any treatmeat; LPS group:cardiomyocytes were exposed to 1 μg/ml LPS for 2 h; YC-1 + LPS group:cardiomyocytes were exposed to 4 μmol/L YC-1 for 8 h before LPS treatment; YC-1 group:cardiomyocytes were exposed to 4μmol/L YC-1 for 8 h.As indexes of mitochondrial function,MMP,the content of ATP and the activity of cytochrome oxidase were measured,COX1/2 protein expression was determined by Western blotting.Results Compared with control group,LPS decreased MMP,the content of ATP and the activity of COX obviously ( P ＜0.05 ),depressed the expression of COX- 1 protein ( P ＜ 0.05 ),induced the expression of COX-2 protein ( P ＜ 0.05 ).Compared with the LPS group,MMP,the content of ATP and the activity of COX were markedly attenuated ( P ＜ 0.01 ),the expression of COX-1 was significantly increased and the expression of COX-2 was decreased in theYC-1 +LPS group (P ＜ 0.01 ).ConclusionsYC-1 attributes to the dysfunction of mitochondrial of myocyte by alterating the expression of COX-1 and 2, HIF-1α may attenuate the mitochondrial dysfunction of cadiomyocytes stimulated by LPS.

Adult ; Aged ; Case-Control Studies ; Female ; Humans ; Leukemia, Myeloid, Acute ; metabolism ; pathology ; Male ; Middle Aged ; NF-kappa B ; metabolism ; Neovascularization, Pathologic ; pathology ; Tryptases ; metabolism ; Vascular Endothelial Growth Factor A ; metabolism ; Young Adult

Bdellovibrio bacteria BDF-H16 was isolated from the gut of Carassius auratus gibelio with Aeromonas sobria.Its shape was ob- served by light microscopy,phase-contrast microscopy,electron microscopy and some of its biological characteristics were also studied.It was demonstrated that BDF-H16 was an gram-negative bacterium and had a bacilliform or arc bacilliform shape with a flagellum at one end.Its size was mostly 0.2?m～0.5?m?0.8?m～1.2?m.It had a wide prey area and could lyse all tested gram-negative bacteria and some gram-positive bacteria.The best lysis conditions to Escherichia coli were 6.75?10~9 cfu/mL of prey bacteria concentration,pH7.0～7.5,28℃.It could grow in the solid culture added 0.85%～5.00% NaCl and was inhibited by enrofloxacin and norfloxacin.

Objective: To discuss the therapeutic method of apoplectic patients with language disorders.Methods: Sixty cases of apoplexy were classified into electroacupuncture group of 30 cases and acupuncture group of 30 cases. In electroacupuncture group, acupoints Lianquan (CV 23), Tongli (HT 5) and lower 2/5 part of motor area and sensory area were selected. In acupunc -ture group, acupoints Lianquan (CV 23) and Tongli (HT 5) were selected. Results and conclusions: After 2 courses of treatment, the total effective rate in electroacu -puncture group was 93.3% and that in acupuncture group was 76.6%. There was a significant difference in the therapeutic effect between the two groups (P＜0.05).

The aim of this study was to investigate the expression of basic fibroblast growth factor (b FGF) and its relationship with tumor microvasogenesis and biological behavior of human renal carcinoma. Gene expression of b FGF was assayed in 20 cases of renal carcinoma tissue using RNA dot blot hybridization. Meanwhile, the protein expression of b FGF and microvasular density (MVD) was assayed in 35 cases of renal carcinoma tissue with immunohistochemical assay. It was found that the expression of b FGF was significantly higher in renal cancer tissue than in normal renal tissue or normal tissue adjacent to the tumor, There was significant correlation between b FGF expression in cancer tissue and clinical stages as well as clinical grades.Similar results were also observed between MVD and b FGF expression in renal cancer tissue. It seemed that b FGF played an important role in angiogenesis in renal carcinoma. Taken together, the results suggest that MVD and the expression of b FGF in renal cancer tissue might be related to the prognosis of renal carcinoma patients.

AIM: To investigate the relationship between alteration of CHOP/GADD153 protein expression and cardiomyocyte apoptosis induced by angiotensin Ⅱ (AngⅡ), and inhibitory effects of CHOP/GADD153 antisense oligodeoxynucleotide (anti-ODN) on apoptosis in vitro.METHODS: Cultured neonatal cardiomyocytes were exposed to AngⅡ with or without preincubation of CHOP/GADD153 anti-ODN. Variability of myocytes was measured by MTT assay, the lactate dehydrogenase (LDH) release was also detected, the percentage of annexin V positive myocytes was monitored by flow cytometry as apoptosis rate, CHOP/GADD153, Bcl-2 and Bax expressions were determined by Western blotting. RESULTS: Compared with control group, the expression of CHOP/GADD153 was obviously increased from (0.20?0.02 to 0.75?0.06) in AngⅡ group (P

Purpose:To analyze retrospectively the clinical manifestation, treatment and prognosis. Methods:From 1978 to 1996,16 patients with olfactory neuroblastoma were treated.4 cases were stage A,6 cases were stage B and 6 cases were stage C. Six patients were treated with radiotherapy alone or combined with chemotherapy, 9, with surgery and postoperative radiotherapy and 1,with operative alone. The radiation dosage ranged from 40～70Gy with 2 Gy per day. Results:The 1 , 3 and 5 year survival rates were 75%, 75% and 69%.Conclusions:Olfactory neuroblastoma is sensitivity for radiotherapy, for the patients with stage A, radiotherapy alone is effective for stage B and C olfactory neuroblastoma , combined modality of surgery and postoperative radiotherapy should be the treatment of choice.

AIM: To observe the efficacy of intravitreal injection with ranibizumab combined with laser photocoagulation for macular edema ( ME ) secondary to macular branch retinal vein occlusion( MBRVO) .
METHODS:A retrospective analysis included 33 patients (33 eyes) with ME secondary to MBRVO were taken. All patients received intravitreal injection of 0. 5mg ranibizumab ( 0. 05ml ) at first visit. The continue PRN treatment and laser photocoagulation were based on the visual acuity changes and optical coherence tomography findings. The changes of best corrected visual acuity ( BCVA) , central macular thickness( CMT) , and amplitude density and latency of P1 wave in mfERG were observed before treatment and 6mo after treatment.
RESULTS: Before the treatment, logMAR was 0. 68±0.35, 6mo after treatment was 0. 34±0. 23, BCVA was improved obviously ( P < 0. 01 ), BCVA in 21 patients ( 63.64%) were improved in two rows among all the patients. CMT before treatment was(487. 30±63. 58) μm, after treatment was(238. 84±52. 66) μm(P<0. 01). The amplitude densities of P1 wave in ring 1, ring 2 and ring 3 after treatment were significantly increased(all P<0. 01), and the latencies were decrease ( all P < 0. 05 ). The conjunctival hemorrhage was observed in 2 eyes after treatment.
CONCLUSION: Intravitreal injection with ranibizumab combined with laser photocoagulation for ME secondary to MBRVO can reduce the CMT and improve visual function.