Objective To study the protective effect of leuprorelin particle on ovarian reserve function applied before the laparoscopic cystectomy of ovarian endometriosis.Methods 106 patients with endometriosis treated from January 2015 to December 2015 in our hospital were selected.The subjects were divided into two groups according to the random number table, 53 cases of each group.The control group adopted the laparoscopic surgery;the observation group took the leuprorelin particle before the laparoscopic surgery.The operation condition, ovarian hormone and function were observed. Results The operation time and bleeding amount in observation group was better than control group ( P <0.05 ) .After surgery, the exhaust time between two groups had no significantly different.After surgery, the level of follicle stimulating hormone (FSH) in observation group was lower than control group; the level of estradiol (E2) in observation group was higher than control group (P <0.05); the level of luteinizing hormone (LH) between two groups had no significantly different.After surgery, the ovarian volume and antral follicle count ( AFC) in observation group was better than control group (P<0.05).Conclusion Before the laparoscopic cystectomy of ovarian endometriosis, the leuprorelin particle protects the ovarian function and morphology and has less impact in the level of hormone.

According to the anatomic classification,refractive surgery is divided into keratorefractive surgery,phakic refractive surgery and scleral refractive surgery.Most procedures for the correction of ametropia are performed on the cornea,with laser-assisted in situ keratomileusis(LASIK) being the most common one.LASIK has shown its limitations,however.For the correction of higher myopic and hyperopic refractive error,the procedure has considerable undesired effects,such as loss of contrast,glare,and iatrogenic keratectasia.Therefore,a variety of intraocular lens,known as phakic refractive surgery,have been designed to achieve full correction of high myopia and hyperopia without loss of accommodation.Phakic refractive surgery is different from kerato-or scleral refractive surgery in that it involves phakic intraocular lens,non-phakic intraocular lens and clear lens extraction +introcular lens.The author will outline recent progress in phakic intraocular lens and provide unifying concepts that are accepted by ophthalmologists.

BACKGROUND:Wear particles factually serve as a medium carrying extensive information on the wear process involved in hip implants.Moreover,it has been demonstrated that wear particles contribute to the failure of Charnley total hip replacement.OBJECTIVE:To investigate the morphological characteristics of wear particles generated by couples of ultrahigh molecular weight polyethylene(UHMWPE) cups and Al2O3 femoral heads,mounted in a simulator in the presence of distilled water and explore the wear mechanism of UHMWPE cup.DESIGN:Wear tests were performed on a ball-in-socket hip simulator.SETTING:Department of Mechanical Engineering,Xuzhou Institute of Technology.MATERIALS:The molecular weight of UHMWPE selected as cup was 3 000 000.Its density was 0.935 g/cm3,hardness 30 HB,fracture strength 35 MPa,and water absorption 450 MPa,and tenacity 7 MPaM1/2.METHODS:The experiment was performed in the laboratory of tribology,China University of Mining and Technology from December 2003 to July 2005.Hip simulator was used to assess the tribological performance of Al2O3-UHMWPE at room temperature,100 r/min,784 N load for 1.5?106 cycles.Distilled water was used as the lubricant.Wear of UHMWPE acetabulum was measured every 3?105 cycles by taking out the acetabulum from simulator,cleaning in distilled water for 3 minutes in an ultrasonic bath,putting in an oven for 40 minutes at 80 ℃,and cooling off in a desiccator.A BT211D electronic balance(reciprocal sensibility,0.01 mg) was used to measure the weight loss of UHMWPE acetabula according to the reference sample method to calculate the wear.A sample(20 mL) of distilled water lubricant used in test was obtained when the simulator was stopped for gravimetric analysis.Morphology of wear particles in samples and surface of worn UHMWPE acetabula were observed by using scanning electron microscope(SEM).The components of wear particles were determined by X-ray.Detailed measurements of the wear particles distribution were conducted by LS100(R) Laser Particle Size Analyzer(including LS SVM Plus).MAIN OUTCOME MEASURES:Morphology and size distribution of wear particles;Wear mechanism and wear rate of UHMWPE acetabula.RESULTS:The wear particles showed a wide range of particle sizes,varying from 0.3 to 180 ?m,most of which ranged from 5 to 20 ?m.More than 99% of wear particles were UHMWPE particles,which were classified into five groups in terms of their morphological characteristics:spherical particle,tearing particle,lamellar particle,fatigued flaking and rod-shaped particle.Lamellar or cube was the predominant form of Al2O3 ceramic particles with flat surface structure and upright edges.The worn surfaces of UHMWPE cup showed evidence of multidirectional scratching,adhesive and micro-contact fatigue wear,and plastic deformation.CONCLUSION:①The dominant wear mechanisms acting in the UHMWPE cup are abrasive wear,adhesive wear,microcosmic fatigue wear and flaking associated with plastic deformation.②The size of particles accumulating mostly is tiny among the particle groups.

Yersinia pestis is the pathogen of plague which has caused three pandemics in human history. Understanding the evolution of Y. pestis in different plague foci from the genomic point of view is of deep significance in improving detection, identification, prevention and treatment of plague. The results of the study of genomic evolution of Y.pestis and their practical implications are discussed in this paper. [HS(1*2/3]

Objective To investigate the changes of cervical vagal efferent discharge induced by lipopolysaccaride in rats. Methods Ten rats were randomly divided into two groups, receiving lipopolysaccaride (5 mg/kg) or normal saline through intravenous injection. Frequency of cervical vagal efferent discharge was recorded at 30 min, 1, 2 and 4 h after the injection. Results The frequency of cervical vagal efferent discharge was significantly increased after LPS injection (P

Objective To investigate the role of chemokine in inducing peripheral arthritis in spondyloarthropathy(SpA).Methods Microarray was used to screen the candidates of inducing peripheral arthritis in SpA,and enzyme linked immunosorbent assay(ELISA)was used to verify the results.Peripheral blood mononuclear cells(PBMC)from 5 healthy subjects were co-incubated 1.5 hours with synovial fluid of 5 patients with SpA,total RNA were then extracted,and the samples were screened for the target genes by using superarray techniques.ELISA was used to determine CXCL1 protein levels of 54 ankylosing spondylitis(AS)patients and 30 healthy controls,also to determine the CXCL1 levels of synovial fluids from 33 SpA patients and 14 osteoarthritis(OA)patients,and to determine CXCL1 protein levels by ELISA before and after treatment with infliximab.Results The expression of CXCL1,CXCL2,CXCL3 and IL-8 were more than 2 fold higher after stimulated by synovial fluids.However,there was no change in other chemokines and cytokines,such as TNF?,IL-6 and IL-1 after stimulation.The protein level of CXCL1 was significant higher in serum of patients with AS than that of healthy control(P

Objective To discuss the feasibility and safety of minimally invasive direct coronary artery bypass grafting (MIDCABG). Methods A total of 33 patients underwent small incision, off-pump, single-vessel direct coronary artery bypass grafting in this hospital from March 2001 to September 2003. Left anterior small thoracotomy was adopted in 14 patients, whereas partial midline sternotomy was conducted in 19 patients. The left internal mammary artery was anastomosed to the anterior descending artery in 31 patients, construction of the aorta root-anterior descending artery anastomosis using the great saphenous vein was carried out in 1 patient, and the right gastroepiploic artery was anastomosed to the posterior descending artery in 1 patient. Results The operative mortality was a zero. The intraoperative blood loss was 163?120 ml, and the postoperative drainage, 193?169 ml. The assisted respiration time after operation was 6 4?5 5 hours and the duration in ICU was 17 8?4 4 hours. Follow-up for 14 7?7 4 months found no deaths. Conclusions MIDCABG is a safe and reliable procedure, offering minimal invasion, less blood loss and fewer complications.

Objective To clone, locate and differentially analyze the transcription factor Relish gene from Anopheles stephensi, and to examine its signals-modulating action on prophenoloxidase cascade and melanization of Plasmodium yoelii oocysts. Methods Relish cDNA of total mosquitoes was amplified by RT-PCR with degenerated primers. Target PCR product was purified,cloned,sequenced and identified. Special Relish gene was amplified with specific primers from hemocytes or midgut,respectively. Semi-quantitative analysis was made under different feeding conditions. Relish message ribonucleic acid was identified with hybridization in situ. Results One cDNA segment of Relish similar to An.gambiae was acquired from An. stephensi. The same Relish gene was also manifested in the hemocytes and midgut. Marked up-regulation expression of Relish was observed at 6,12,24 or 48 h of Plasmodium yoelii infection and at 12 and 24 h after sucking nitroquine-acetate sucrose solution,that was before inducible oocyst melanization. Relish was also expressed in the hemocytes and midguts by ISH. Conclusion Transcription factor Relish of An. stephensi might play a role in signal modulation of Plasmodium yoelii infection and oocyst melanization.

Objective To investigate the significance of myelography for selecting operation methods in microendoscopic discectomy(MED).Methods A total of 196 patients with lumbar disc herniation diagnosed by CT/MRI received a myelography before MED.According to their clinical manifestations,signs,and outcomes of myelography,the patients were given three different operation methods.①Simple discectomy was performed in 105 patients with patent spinal canal,lateral impression at intervertebral level,and shift of the nerve root sleeve;②Discectomy with spinal decompression was performed in 70 patients with patent spinal canal and compression of the nerve root sleeve;③Discectomy with semilaminectomy was performed in 21 patients with patent spinal canal,semispherical compression of the nerve root sleeve,and 7-shaped nerve root.Results The operation time was 30~90 min(mean,50 min) and the intraoperative blood loss was 30~120 ml(mean,50 ml).No intraoperative complications occurred.Conversions to open surgery were required in 21 patients,including partial spinal stenosis in 16 patients and thickened vertebral plate in 5 patients.Follow-ups were carried out in 196 patients for 3~58 months(mean,34 months).According to the Japanese Orthopedic Association(JOA) scoring system,the final scores significantly increased postoperatively(25.86?2.96) than preoperatively(13.21?4.96)((t=)38.24,P=0.00).According to the Macnab and Watts standard,the rate of excellent or good results was 93.7%(164/175).Conclusions Myelography has an important reference value for selecting operation methods of microendoscopic discectomy in patients with lumbar disc herniation.

In order to solve the problems about the lack of investing capital and to evade investing risk,a military hospital can achieve better economic benefit by introducing cooperative medical items.This method can not only make full use of its own good invisible assets and medical resource,but also help to raise its level of medical service and expand its market of medical service.To make a good item of cooperative medical service,special attentions must be payed to the following aspects such as prophase demonstrating,contract signing,partitionning of income and expenditure,financial accouting and daily accouting.