HIV Infections ; prevention & control ; Humans

Objective To establish and verify a multiplex fluorescence quantitative PCR method for detection of CHO host cell residual DNA content and fragmentation degree, so as to provide a reliable method for safety detection of related vaccines.Methods Two sets of primers(Alu-L and Alu) and universal probe were designed for clone250 and clone49c, members of Alu sequence family of CHO cells. The UBI3 gene of capsicum was introduced as external standard gene, and corresponding primers and probe were designed. Multiplex reaction system of Alu sequence and external standard gene was established, and the residual DNA of CHO cells in samples was quantified by the multiplex fluorescence quantitative PCR. The linear range,limit of quantitation(LOQ), specificity, robustness, precision and accuracy of the method were verified. In addition, the DNA fragmentation degree of CHO cells was determined by comparing the △Ct [Ct(Alu-L)-Ct(Alu)] of the amplification curves of long and short primers of Alu sequences. Results The multiplex reaction standard curve had a good linear relationship in the range of 0. 001-0. 1 ng/μL, R~2≥ 0. 99. The addition of external standard gene had no effect on the detection ability for CHO target, and the LOQ was 0. 5 fg/μL. The DNA of human, E.coli, rats and mice had no effect on the detection, the primers targeting residual DNA of CHO cells had no specific amplification in the genomes of seven animal cell lines, and the fragmented DNA samples showed no effect on the detection results. The CVs of precision verification were all less than 15%, and the recoveries of simulated samples in normal saline and PBS containing 1% BSA were in the range of 70%-130%. The△Ct of Alu and Alu-L amplification curves increased with the degree of sample fragmentation, and the fitting curve R~2≥ 0. 99.Conclusion The multiplex fluorescence quantitative PCR detection method established in this study can rapidly and accurately quantify the residual DNA of CHO cells and determine the fragmentation degree of samples according to △Ct, which can be used for the safety detection of the related vaccines.

Liquichip technology utilizes different fluorescent coding microbead as carriers of bio-probes and flow cytometry as optical detection method,to accomplish bio-molecules reaction in suspension liquid system.This article introduces the application of liquidchip technology in the analysis of autoimmune disease,cytokine,contagious disease,endocrinopathy,neural disease,tumor marker detection and so on.With the advantages of high sensitivity,high speed,high flux,multi-analyte,high accuracy and excellent repeatability,liquichip technology has promising prospect to be widely used as advanced biomedical detection platform.

Antipsychotic Agents ; therapeutic use ; Brain ; physiopathology ; Cognition Disorders ; genetics ; Gene-Environment Interaction ; Genetic Variation ; genetics ; Humans ; Schizophrenia ; genetics ; physiopathology ; Schizophrenic Psychology ; Social Behavior ; Thinking ; physiology

Cochlear Implants ; Magnetic Resonance Imaging ; Positron-Emission Tomography

Aim To study whether or not the green tea catechins(EGCG)has physiological benefits and the underling protective mechanism.Methods The subunit protein levels of ?4、?7 of nAChR were detected by BCA protein assay,Dot Blot assay and MTT assay.Results The results showed that the green tea catechins can significantly reduce the subunit protein levels of nAChR and decrease the cell activity induced by A?1-40.Conclusions EGCG can provide neuroprotection in vitro by up-regulating nAChR sununit levels and inhibiting the neurotoxin of A?1-40.

Objective To study the inhibitory effect of yan xiao capsules on hepatocarcinoma mice. Methods Forty mice were randomly divided into control group ,CTX group,ADM group,trial group. Observe tumour weight ,the span time of tumour-bearing mice, tumour morphology and the mortality rate of ascites cancer cell by direct killing respectively. Results The tumour weight markedly decreased in trial group, the rate of inhibition tumour was 52.0 %(P

Expansive type of hepatocellular carcinoma (HCC) is a subtype of HCC. Correlation between the preoperative sonography and postoperative pathology was studied in 35 cases of HCC. According to the classification of nakashima, 21 cases belonged to the expansive type whereas the other 14 cases the infiltrative type. Different from the infiltrative type. the expansive type of HCC had four specific sonographic appearances: hypoechoic ring, hypoechcic halo, pedunculated and small nodular echo which had different pathological bases. Expansive tyje of HCC was not so commonly seen as infiltative type but its rate of surgical resection was mu~h higher than that of infiltratine type (JP< 0. 01). Thus, it is the kind of HCC with significant clinical importance. Preoperative ultrasonographic diagnosis help to decide the way of treatment.

At present clinic study and use of anti-tumour platinum drug are wide and active. The paper reviews progression of clinic study and use of anti-tumour platinum drug and expects its prospect.

Objective To investigate the relationship of self-management skills and the quality of life among solitary elderly patients with hypertension.Methods 110 cases of solitary elderly patients with hypertension were measured with self-designed Self-management of Hypertension Scale and Short Form Health Survey (SF-36) and the correlation of them was analyzed.Results The levels of self-management skills of elderly hypertensive patients was at a middle level,and the mean score of self-management skills was (78.6 ± 5.2) points.The total score of SF-36 of patients with good self-management skills was higher than those with bad self-management skills.The therapy management,catering management,psychosocial management,sports management and overall quality of self-management of elderly patients with hypertension were positively correlated with survival quality.The factors influencing self-management ability of patients incuded education,gender,social resource utilization,psychological conditions.Conclusions Community health nurses should pay attention to self-management skills such as diet,exercise,therapy,social and psychological aspects in elderly hypertensive patients in order to improve their quality of life.Furthermore community nurses should strengthen intervention for solitary eldedy males with lower education level,poor utilization of social resources to improve their self-management skills.