Reverse transcription loop-mediated isothermal amplification(RT-LAMP)is a novel nucleic acid amplification technique which has been mainly used in a variety of viruses and other pathogens of gene detection.Compared with reverse transcriptase polymerase chain reaction (RT-PCR),the RT-LAMP has advantages of reaction simplicity,rapidly,cost-effective,detection sensitivity and specificity.Nowadays,RT-LAMP technology is gradually used in various medical fields,but it is still a new method especially in cancer diagnosis of tumor gene.The article reviews the application of the RT-LAMP technology in the cancer

Researches indicate that gastric carcinoma occurs where gastric stem cells settle down.Stem cells may play an important role in formative process of gastric carcinoma. Researches on relationship between stem cells and gastric stem cells, tumor stem cells and stomach neoplasms, bone marrow stem cells and stomach neoplasms indicate that gastric carcinoma may be a kind of stem cell diseases.

Objective To explore the correlation between serum HBV-DNA content and hepatic fibrosis indicators in hchronic epatitis B patients.Methods Serum HBV-DNA content was determined by using real-time fluorescence quantitative PCR and he-patic fibrosis indicators including hyaluronic acid,laminin,procollagen Ⅲ,and collagen Ⅳ which were determined by using chemilu-minescence.Results The logarithm value of serum HBV-DNA content of the 110 hepatitis B patients was 5.32±1.37.Serum hyal-uronic acid concentration was (197.81 ±85.37)mg/mL;procollagen Ⅲ concentration was (142.66 ±30.28)μg/mL.Collagen Ⅳconcentration was (90.34 ±20.53)μg/mL.Laminin was (143.96 ±31.79 )μg/mL.All these indicators were significantly higher than the control group (P <0.01).But there were no significant linear correlation between serum HBV-DNA content and the four hepatic fibrosis indicators(P >0.05).Conclusion The hepatic fibrosis indicators increased significantly in hepatitis B patients but there were no significant correlations between serum HBV-DNA content and the hepatic fibrosis indicators.

Objective To explore the establishment methods of animal models of intestinal multidrug-resistant bacterial (Pseudomonas aeruginosa, PA) infection, and to provide a good model for experimental research and treatment for infection caused by drug-resistant bacteria .Methods Male BALB/c mice were divided into four groups:Normal con-trol group, MDR-PA group, MDR-PA +antibiotics group, and MDR-PA +fasting group.They were given saline, PA suspension, PA suspension after free drinking with ceftriaxone water and PA suspension after fasting , respectively , by ga-vage.Colon tissues were collected for pathological examination and inflammation scoring .The expressions of TNF-αand INF-γin the colon tissue was assessed at the end of the experiment .Results Pathology showed different degrees of inflam-matory changes in the colon tissues .Compared with the normal control group , the expressions of TNF-αand INF-γhad sig-nificant differences with that of experimental groups .Conclusions All the three methods used in this experiment can be successfully used to establish mouse models with intestinal infection caused by multidrug-resistant Pseudomonas aeruginosa, and may be chosen for experimental studies according to different research purposes .

Objective To investigate the expressions of SOCS3 and IL‐6 in triple‐negative breast cancer(TNBC)and their clinical significance.Methods The expressions of SOCS3 and IL‐6 in TNBC and normal breast cells were detected by Western blot and fluorescence quantitative PCR.Immunohistochemical staining was used to detect their expressions in 84 TNBCs ,33 breast fibromas and 11 normal breast tissues. The correlation of their expressions in TNBC and the relationship between their expressions and clinical pathological parameters were analyzed as well.Results SOCS3 was not expressed or down‐expressed in TNBC cell lines while IL‐6 was over‐expressed. The positive rate of SOCS3 protein was 32.1% in TNBCs ,63.6% in breast fi‐bromas(P<0.01)and 72.7% in normal breast tissues (P< 0.01);and that of IL‐6 protein was 72.6% in TNBCs ,30.3% in breast fibromas(P< 0.01)and 27.3% in normal breast tissues (P< 0.01).Moreover ,a significant negative correlation was found between the expression of SOCS3 and that of IL‐6 in TNBC(rs = -0.590 ,P<0.01). The expressions of SOCS3 and IL‐6 were markedly associated with tumor size ,lymph node metastasis ,clinical stages and expressions of Ki‐67 and p53(P<0.05) , but not with age ,histological grades or menopausal status(P>0.05).Conclusion The expressions of SOCS3 and IL‐6 are asso‐ciated with the development of TNBC ,suggesting that SOCS3 and IL‐6 can serve as biological markers for evaluating the prog‐nosis ,and therapeutic targets of T NBC.

ObjectiveTo explore the roles of Toll-like receptor (TLR) 3,7 and 9 in the pathogenesis of pityriasis rosea.MethodsThis study recruited 40 patients with pityriasis rosea and 40 normal human controls.Reverse transcription-PCR was carried out to detect the expressions of TLR3,7 and 9 in peripheral blood lymphocytes of these subjects.ResultsThe mRNA expressions of TLR3,7 and 9 in peripheral blood lymphocytes were significantly higher in patients with pityriasis rosea than in the normal human controls (Z =4.239,3.745,2.658,all P ＜ 0.05).There was a positive correlation between the expression of TLR3 and TLR7(r =0.574,P ＜ 0.01 ) as well as between that of TLR3 and TLR9 (r =0.551,P ＜ 0.01 ) in the patients,while there was no correlation between TLR7 and TLR9 expression in the patients or between the expressions of TLR3,TLR7 and TLR9 in the controls.ConclusionVirus infection might be involved in the pathogenesis of pityriasis rosea via TLR pathways.

Homebox genes are known to determine cell proliferation and differentiation and subsequently regulate the occurrence,development and prognosis of many tyhpes of malignant tumors in human.Some recent studies have shown that homebox genes are abnormally expressed in the esophageal,gastric,coloreetal and other gastrointestinal tumors.In this review,we consider that homebox genes may regulate the occurrence,development of gastrointestinal tumors by interacting with transcription factors in vivo and abnormal epigenetic modifications.Therefore,homebox genes are closely related to gastrointestinal tumors.

Chemotherapy resistance is a great obstacle for effective treatment of ovarian cancer.Drug resistance of ovarian cancer is the results of combined action of multi- factors.Further study of drug resistancerelated genes can provide new ideas for reversing drug resistance fundamentally.

BACKGROUND:Studies have found that the glass fiber splint which is embedded into the groove on the lingual surface of the affected tooth plus fluid resin fixation between the gap of mobile teeth can achieve more satisfactory clinical effects on the stabilization of loose front teeth.
OBJECTIVE:To observe the clinical effects of glass fiber splints prepared using two tooth preparation methods on splinting loose front teeth and their debonding rate through a 5-year fol ow-up visit.
METHODS:Ninety-four chronic periodontal disease patients, who had received periodontal treatment, were enrol ed in this study for their loose front teeth of degree II-III. Al the enrol ed patients were good candidates for periodontal splinting strictly in accordance with the indications for periodontal splinting. According to whether the fluid resin was used to seal the gap between the mobile teeth, the 96 patients were divided into two groups:patients undergoing glass fiber splint+fluid resin between the mobile teeth served as experimental group, and those only undergoing glass fiber splint as control group. Al the patients were fol owed by regular clinical referral or telephone fol ow-up for 5 year, to evaluate the fixed effects.
RESULTS AND CONCLUSION:During the 5 years of regular clinical referral or telephone fol ow-up, seven of the 76 cases and 12 of the 18 cases were found in the experimental and control groups, respectively, to have debonding between the abutment and the splint. Compared with the control group, the probing depth and attachment loss of affected teeth were improved significantly (P<0.05), and the percentage of alveolar bone height accounting for the root length increased significantly in the experimental group (P<0.05) after 5 years of fol ow-up. Patients in the experimental group felt comfortable without foreign body sensation. The results confirmed that the glass fiber splinting plus fluid resin fixation between the gap of mobile teeth was a better treatment for loose front teeth during the 5-year fol ow-up.

Objective: Our aim was to evaluate the biocompability of porcelain compounded with nanostructured material. Methods：The proliferation of the NIH3T3 cells were investigated using a tetrazolium-based colorimetric assay(MTT assay).Maceration extract of four tested materials were added into culture plate .The samples were tested with ultraviolet spectrophotometer in 490 wavelength at 1，3，5，7 days after the addition of extract. Morphology of these cells was also observed with phase-contrast microscope. Results： There was no obvious difference among three kinds of prosthetic materials in the optical absorbance and the cells growing in the extract showed normal morphology. Conclusion: The toxicity graduation of the porcelain compounded with nanostructured material is 0. This material has a good biocompability and can be used clinically.