Objective To investigate the diagnosis and treatment of acute acalculous gangrenous cholecystitis (AAGC). Methods The clinical data of 46 patients with AAGC treated in our hospital from 1990 to 2006 were retrospocfively analyzed. Results All patients with AAGC were surgically treated when the final diagnosis was confirmed. Of the 46 patients, 32 underwent cholecystectomy, 14 partial cholecystectomy and 5 cholecystectomy, exploration of common bile duct and T-tube drainage. Tow patients died after operation due to toxic shock and multiple organ failure. Conclusions B-mode ultrasonography, laboratory examination, symptoms and clinical signs are the main methods for diagnosis of AAGC. Early diagnosis, careful preoperative preparation and surgical therapy are important to raise curative rate of the disease.

Objective To investigate changes in indicators related to inflammatory cytokines, coagulation and fibrinolysis system in patients with disseminated intravascular coagulation (DIC) and multiple organ dysfunction syndrome (MODS) resulted from sepsis. Methods In total, 97 patients diagnosed as sepsis were divided into different groups and their plasma concentrations of tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), tissue factor (TF) and tissue factor pathway inhibitor (TFPI), tissue plasminogen activator (t-PA), and plasminogen activator inhibitor 1 (PAI-1) were measured by ELISA and plasma activity of protein C (PC:A) was measured by chromogenic substrate assay. Results Plasma concentrations of TNF-α and I L-6 were (38 ± 12) ng/L and (77 ± 9) ng/L, respectively, in patients of sepsis complicated with DIC, much higher than those without DIC [ ( 17±6) ng/L and (45 ± 6), respectively], P <0.05;and (63±25) ng/L and (103±28) ng/L, respectively, in those complicated with MODS, significantly higher than in those without MODS [ (29 ± 7 ) ng/L and (48±9)ng/L, respectively], P < 0/05 and those without DIC [( 17 ± 6) ng/L and (45 ± 6) ng/L, respectively], P <0. 05;as well as significantly higher in the dead than in survivors (P <0. 05). Plasma activity of protein C was (32 ± 10) percent in those with DIC and (24 ± 12) percent in those with MODS, both significantly lower than in those without DIC [ (57±28) percent] and without MODS [ (55 ± 17) percent], respectively, P <0. 05, as well as significantly lower in the dead than in survivors, P <0. 05. Plasma concentrations of t-PA and PAI-1 were significantly higher in sepsis patients with DIC [(48±17)μg/L] than that in those without DIC [(103 ± 38)μg/L], P < 0.05. Conclusions Inflammatory cytokines play important roles in development of DIC as well as MODS in patients with sepsis. Decreased activity of protein C and increased plasma level of PAI-1 can result in deposition of fibrin on the vessel wall and thrombosis, which can be used as indicators of poor prognosis for patients with DIC.

Objective To summarize the experience of diagnosis and treatment of gastric non-Hodgkin's lymphoma of mucosa-associated lymphoid tissue.Methods Twenty-seven patients,proved by pathology,were included in the study.Results Among clinical presentations,the upper abdominal pain,intestinal bleeding,and weight loss were common.Only 1 case was diagnosed definitely from 18 cases with the examination of X-ray barium meal,84.6%(24 of 26 cases)were miss-diagnosed under gastroscopy.All cases underwent operation,among them 25 performed a radical operation.Twenty-four patients were followed up.Conclusion The multiple biopsy sampling from submueosal layer via gastroscope may improve diagnostic rate on gastric non-Hodgkin's lymphoma of mucosa-associated lymphoid tissue.Operative removal of the tumor should be the first choice of treatment.Additional chemotherapy after the surgery increases survival rate.

Objective To explore the clinical features and treatment of the cystic change of thyroid canc-er. Methods A retrospective analysis was made based on the clinical data of 25 patients with cystic change of thyroid cancer from 1994 to 2008. Results lacking the specificity in clinical diagnose, the di-agnosis accuracy of the disease before operation was very low and the misdiagnosis rate was very high. A-mong 25 cases, only one was accurately diagnosed before operation, 3 were reoperated after the accurate di-agnosis by using intra-operative frozen section examination. Conclusions Clinical physicians should raise their awareness to such a disease. To improve the accurate diagnosis of the misdiagnosis of the cystic degen-eration of thyroid cancer, clinical physicians should integrate the examinations including B-ultrasound, preop-erative fine-needle aspiration biopsy,and intra-operative frozen pathological section.

Study on Rank—Frequency Distribution of Scientific Research Papers by Chinese University famous teacher teaching in biomedicine domain from 2003 to 2009.Whose quantitative indicators of scientific papers were negative logarithmic distribution,except provincial-level teaching famous teachers' SCI papers were negative exponential distribution. The qualitative index were negative logarithmic,except state-level teaching famous teachers' SCI paper yearly average paper cited were linear distribution and provincial-level were negative exponent distribution.The magnitude of concentrations was between 0.257 and 0.728,which deviates from the Price's law (0.50). It indicates that the Rank-frequency distribution model can be applied to the teaching famous teacher.The internal gradation instrument in the top scientists is different from in science community.Scientist's gradation in the science community depends on whether the objective of scientific paper evaluation comes true.The state-level teaching famous teacher scientific paper difference is greater than the provincial-level; SCI paper difference is bigger than the CSCD paper.

The prevalence of maternal obesity has risen dramatically in recent years. Maternal obesity increases the risk of adverse pregnancy outcomes, and can lead to chronic inflammation, oxidative stress, the change of cell factor homeostasis. The metabolic status of maternal obesity in the pre-pregnancy and pregnancy can alter the level of DNA methylation in the placenta, change the fetal programming, influence the pregnancy outcomes, and increase the risk of obesity related metabolic syndrome and other chronic diseases of offspring. Actively preventing and intervening in the maternal obesity can reduce the adverse pregnancy outcomes and increase the survival quality of the offspring.

BACKGROUND:The establishment of model is very crucial in the orthodontic tooth movement experiment. The selection of force and control ing of anchorage may greatly affect experimental results.
OBJECTIVE:To establish an animal model for orthodontic tooth movement.
METHODS:Twenty Wistar rats were included in this study. The root of incisor teeth at upper jaw was dril ed and then threaded with a ligature wire (0.3 mm diameter), therefore the alveolar bone and two incisor teeth were ligated. The first molar at right upper jaw was also ligated using a ligature wire (0.2 mm diameter). The experimental teeth were randomly divided into four groups. A Sental oy closed-coil spring (10 g, 25 g, 50 g) was placed between the maxil ary incisors and the maxil ary first molar, respectively. Taking the incisor as the anchorage, the molars were given a mesial movement, and control group received no force. On day 14, the dental pulp, dentin and enamin resorption was analyzed with Image-Pro Plus software.
RESULTS AND CONCLUSION:A light force (10 g Ni-Ti coil spring) produced irregular enamel resorption and narrowed periodontal membrane space, whereas heavy force (25 g Ni-Ti coil spring) produced irregular dentin and enamel resorption, even heavier force (50 g Ni-Ti coil spring) produced apparent resorption at dental root, which affected the dentin. No statistical difference of the relative depth of dentin resorption was found between the 10 g group and the control group (P>0.05). The data between other groups showed statistical y significant differences (P<0.05). The mechanical union of the incisors and the mandibular bone efficiently prevents the continuous eruption of the upper incisors, which maintains the anchorage stability. Using Ni-Ti coil spring, a stable, scientific and reliable model of orthodontic tooth movement can be established through mesial movement of the first molars. The optimal force of 10 g is used to move the rat first molar mesial y.

Shu Xin Yi Mai Capsule combined with Western medical routine treatment was used for 22 successful cases of coronary intervention treatment ( Chinese - Western Medicine group) and 28 cases routinely treated with simple Western medicine were used as control (Western medicine group). Recurrence rales of angina pectoris before and after treatment were observed. Results indicated that the recurrence rate in the Chinese -Western medicine group was significantly lower than that in the Western medicine group GP

Animals ; Endothelium, Vascular ; Humans ; Lipid Metabolism ; Metabolic Diseases

Objective To observe the effect of PKB gene silencing on the growth of gastric cancer cell line SGC-7901 in vitro.Methods Gene transfection technique was used to transfect AKt2 siRNA into gastric cancer cells.Akt2 expression was detected by RNAi technique,Akt2 protein level was detected by Western blot,and the change of cell cycle distribution and apoptosis of SGC-7901 cells were detected by flow-cytometry,SGC-7901 proliferation was measured by MTT method.Results After SGC-7901 cells transfected with Akt2 siRNA,the expression of protein level decreased obviously(P