Objective To analysis the hot spots of innovative technology of Chinese medicine industry in Shanghai during the recent years.Methods Patent Database of State Intelletual Property Office was retrieved by IPC class numbers combined with keywords and the IPC classification of the Chinese medicine patents in Shanghai area during 2002-2013 were under statistical analysis.Results The hot spot distribution of therapeutic activity of TCM preparations:Antineoplastic is the most important activity of Chinese medicine development, followed by the activities of treatment of inadequate blood flow or cardiovascular system diseases, such as hyperglycemia, high cholesterol, and so on. The activity of treatment of the digestive tract or digestive system diseases, followed by the activities of anti infection, antiviral, antibacterial, anti-inflammatory treatment, etc. The hot spot distribution of dosage:Researches on new dosage of Chinese medicine were mainly reflected in the improvement of traditional dosage form and development of new formulations. The capsules, tablets, oral liquid, and injections were the new dosage forms under the most researches. The distribution of medical resources:Medicinal plants or animals mainly came from “angiosperm”. Medicinal animals were less than medicinal plants, which mainly belonged to insecta.ConclusionThe hot spots of innovative technology of Chinese medicine industry in Shanghai are mainly distributed in the medicine of anti-tumor, cardiocerebral vascular diseases, digestive system diseases and so on in recent years. The researches of new formulation focus on the traditional dosage form improvement and new formulations development. “Angiosperm” is the key to the development of medicinal plants.

BACKGROUND: The coating induced by conventional simulated body fluid (SBF) and 1.5-folds SBF as biomimetic method has the shortcomings of slowly growth with weeks or months growth cycle. OBJECTIVE: To evaluate Ti-6Al-4V alloy surface deposition of hydroxyapatite coating induced by 3-folds SBF. DESIGN, TIME AND SETTING: In vitro biological analysis. The experiment was performed at the Institute of Metal Research, Chinese Academy of Sciences from November 2008 to March 2009. MATERIALS: Ti-6A1-4V alloy was supported by Boda Metel Materials Co., Ltd. METHODS: The pretreated Ti-6Al-4V alloy was immersed in 37 'C 3-folds SBF. The SBF was replaced every 24 hours for 3 days. MAIN OUTCOME MEASURES: The morphology, phase and elemental composition of calcium phosphate coating were analyzed by scanning electron microscopy, X-ray diffraction and infrared spectroscopy analysis. RESULTS: The scanning electron microscopy exhibited that the surface of Ti-6A1 -4V alloy was covered with deposited film with granular crystal on local areas at day 1 after immersion. The deposited film covered surface of Ti-6A1-4V alloy at day 3 after immersion, and granular or scale-shaped crystal could be seen. The crystal deposition displayed sustained growth with certain area as center. The product comprised Ca, P, O, C elements without Al, V. X-ray diffraction and infrared spectroscopy analysis revealed that the main component of the coating was hydroxyapatite. CONCLUSION: The 3-folds SBF can accelerate the deposition as well as shorten formation duration of hydroxyapatite coating on Ti-6A1-4V alloy.

BACKGROUND:At present, the study to balance ability of functional ankle instability patients is less. The
balance ability deficiency may also be one of the important reasons for ankle repeated injury, so the research can effectively reduce the damage probability.
OBJECTIVE:To explore the static balance ability characteristics of functional ankle instability patients.
METHODS:Ten male subjects with unilateral functional ankle instability were enrol ed as the experimental group in the study, and other 10 healthy match-paired subjects were served as the control group. Their static balance ability was measured under double feet standing and single foot standing using win-pod system, the double feet testing lasted for 30 seconds and the single foot testing lasted for 10 seconds. Evaluation parameters of balance ability were obtained.
RESULTS AND CONCLUSION:During double feet testing, there were no significant differences in balance
ability parameters between the experimental group and the control group whether eyes be opened or closed (P>0.05), but the index values of double feet testing were obviously less than those of single foot testing. So we
concluded that in the double feet standing, there was no significant difference in the static balance ability between functional ankle instability patients and normal persons. Moreover, the static balance ability of double feet was
better than that of single feet. In the single foot standing of the affected foot, the static balance ability of functional ankle instability patients was slightly worse than that of the normal persons, especial y with eyes closed.
Comparison of the average swing between the X-axis and Y-axis found that in the process of maintaining balance posture, the swing direction of the body center of gravity was mainly in the anteroposterior direction.

Objective To explore whether the inhibited expression of fibrocystin by RNA interference can increase epidermal growth factor (EGF)-induced cell proliferation and its possible mechanism . Methods A stable PKHD1-silenced HEK 293 cell line was established . Cell proliferation rate, intracellular Ca2+ concentration and extracellular signal-reguhted kinase 1/2(ERK1/2) activity were assessed after treatment with EGF, verapamil and Bay K8644 . Results The proliferation rate of PKHD1-silenced HEK-293 cells was found to be significantly higher after EGF stimulation compared to the control HEK 293 cell (231 .5% vs 152 .8%, P＜0 .01) . PKHD1-silencing lowered the intracellular Ca2+ concentration and caused EGF-induced ERK1/2 overactivation in the cells(P＜0 .01 ) . When cells were treated with verapamil for 4 hours to lower the intracellular Ca2+ concentration, the cell proliferation rate was significantly increased after 20 ng EGF for 24 hours . The verapamil treatment increased the level of activated ERK1/2 in EGF-treated cells . An increase of intracellular Ca2 + in PKHD1-silenced ceils repressed the EGF-dependent ERK1/2 activation and the hyperproliferative response to EGF stimulation . Conclusions Inhibition of fibrocystin can cause EGF-induced excessive proliferation through decreasing intracellular Ca2+ resulting in EGF-induced ERK1/2 activation . The loss of fibrocystin may lead to abnormal proliferation in kidney epithelial cells and cyst formation in ARPKD through modulation of intracellular Ca2+ concentration .

Objective To explore the clinical application effect of Shuxuening combined with ozagrel sodium in the treatment of cerebral infarction patients with psychological intervention.Methods120 patients with cerebral infarction were randomly divided into observation group and control group from January 2015 to June 2016.The control group was treated with intravenous ozagrel sodium and psychological intervention.The observation group was treated with control group based on the use of Shuxuening.ResultsThe clinical efficacy in the observation group was significantly higher than in the control group (P<0.05).The incidence of complications in the observation group was significantly lower than that in the control group (P<0.05).ConclusionThe combination of Shuxuening combined with ozagrel sodium and psychological intervention in the treatment of cerebral infarction, can effectively improve the patient's treatment efficiency, improve the physiological and psychological status of patients, and effectively reduce the treatment of patients with complications.

Objective To discuss the correlation between the dysmenorrhea and TCM constitutions among the junior high school girls of Shanghai suburb; To analyze the influencing factors; To provide references for the prevention of dysmenorrhea. Methods By using cluster sampling during December 2015 to January 2016, the questionnaire surveys which included the general situation, the classification and determination of TCM constitution self-test and COX Menstrual Symptom Scale were used for the junior high school girls from the suburb area of Shanghai to analyze the relationship between dysmenorrhea and TCM constitutions and the influencing factors. Results The incidence rate of dysmenorrhea was 29.52% (93/315) in junior high school girls from the suburb area of Shanghai. There was statistical significance in body height and menarche age among girls with and without dysmenorrhea (P<0.05). The rank correlation coefficient of dysmenorrhea and gentleness type was -0.130 (P=0.021). The rank correlation coefficients of dysmenorrhea and pathological constitution (except for special diathesis type) were all greater than 0 (P<0.05). Univariate analysis showed that there was statistical significance in the incidence of different coagula in menses, different inheritance of dysmenorrhea, different sleep quality, different learning pressure, different emotional state and different physical exercises (P<0.05). Logistic regression analysis showed that influence factors of dysmenorrhea were inheritance of dysmenorrhea, learning pressure and yin-deficiency constitution. Conclusion The pathological constitution is the susceptible constitution of dysmenorrhea of junior high school girls. Yin-deficiency constitution, inheritance of dysmenorrhea and learning pressure can increase the incidence of dysmenorrhea. It is suggested that the corresponding preventive measures should be taken to achieve the purpose of prevention dysmenorrhea by regulating and improving yin-deficiency constitution.

Objective To analyze the incidence and related risk factors of chronic obstructive pulmonary disease (COPD) patients with deep venous thrombosis (DVT) in the respiratory intensive care unit (RICU).Methods We recruited 204 patients from RICU of our hospital between January 2015 and December 2015.The basic clinical data of the patients were also collected.Bedside venous ultrasonography of double lower limbs was taken 24-48 hours after admission.The patients were divided into DVT group and non-DVT group.The clinical data of the two groups were analyzed and compared using Chi-square test or t test,Logistic regression analysis was used to analyze risk factors,and ROC curve analysis was used to identify the disease.Results Among the 204 patients,39 were identified with DVT,with the overall incidence rate of 19.1％.D-dimer in DVT group was significantly higher than that in non-DVT group (P＜0.001).The two groups did not significantly differ in age,sex,smoking history,underlying diseases,or other laboratory test results.The area under the ROC curve of D-dimer was 0.787,and the cut off value was 1.985 mg/L (sensitivity of 0.641,specificity of 0.817).Conclusion D-dimer is closely associated with increased incidence of DVT in COPD patients.

Objective To investigate the clinical effect of laparoscopy combined with leuprolide on patients with mild endometriosis and infertility and effects on fertility function of patients. Methods A total of 60 patients with mild endometriosis and infertility in our hospital were randomly divided into the control group (n=30) and the observation group (n=30). The control group was treated with laparoscopic surgery, the observation group was treated with leuprorelin treatment, and the levels of FSH, E2, luteinizing hormone and PROG were tested by automatic biochemical analyzer between two groups before and after treatment, the pregnancy rate as assessment of reproductive function in patients. Clinical curative effect and effect on fertility of 2 groups were compared. Results Levels of FSH, E2, LH and PROG in the observation group were lower than those in the control group (P<0.05) in 3 months after treatment, and the pregnancy rates in the observation group after 1 months, 2 months and 3 months were higher than that in the control group (P<0.05). Conclusion The treatment of Laparoscopy combined with Leuprolide for mild endometriosis and infertility in patients has ideal effect, and is worthy of popularization and application.

Objective To investigate the expression and effect of zinc-finger protein kuppel-like factor4(KLF4) in leukemic cell line K562. Methods The lever of mRNA and protein of KLF4 is determined with real-time PCR and Western blot in human peripheral monoeyte/macrophage and K562 cells. Results Compared with control group (0.43±0.03), the expression of KLF4 mRNA in K562 cells (0.02±0.01) wassignificant different(P＜0,05). The expression of KLF4 protein in human peripheral monocyte macrophage is higher than that in K562 cells. Conclusion The low expression of KLF4 was present in leukemic cell line K562,which may suggest that KLF4 gene maybe a tumor inhibitor factor, and partially involved in leukemogenesis.