2.Exploration of Current Status of Undergraduate Education of Rehabilitation Medicine
Chinese Journal of Rehabilitation Theory and Practice 2011;17(8):788-789
Cultivating talents of rehabilitation medicine is the most urgent affairs of developing rehabilitation medicine in China. The undergraduate education of the rehabilitation medicine is an important link of improving the school-level and the academic position of rehabilitation medical education and cultivating talents of rehabilitation medicine. This article focuses on the present situation about the undergraduate education of rehabilitation medicine, the sources of undergraduate, the location of training targets, the professional fields as well as the problems needing attention and solutions in undergraduate education.
3.Rehabilitation Staffing in Community Health Care Service Center of Guangzhou City
Chinese Journal of Rehabilitation Theory and Practice 2010;16(7):687-689
Objective To investigate the rehabilitation staffing in community health care center in Guangzhou City. MethodsThe basic personal information, work condition, work load, problems in special staff of rehabilitation for disabled persons were investigated with self-report questionnaires. Results143 staff (92.86%) had accepted higher education, 38 (24.68%) practiced beyond 3 years. The main work was the rehabilitation services for local inhabitants, and the other services have not been carried out widely. The main problems reported were lack of finance, staff and skills training. 83.77% promised that they would provide the rehabilitation services meeting the governmental standard. ConclusionStaff who work for the community health care center have more professional education and experience, compared with years ago. The services need more normalization, and the financial support is also needed.
4.The study on the proliferation and the apoptosis factors in vitro of Kölliker organ supporting cells in the cochlea of newborn rat.
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2015;29(2):152-159
OBJECTIVE:
To study the apoptosis/proliferation of Kölliker organ supporting cells and to understand the prompting apoptosis factors in vivo in the supporting cells in the Kölliker organ by changing the environment of the cultured supporting cells in the Kliker organ in vitro, via the separation, culture and purification of the supporting cells in the K6lliker organ.
METHOD:
A combinatorial approach of enzymatic digestion and mechanical separation was employed to isolate and culture in vitro pure Kölliker organ supporting cells. The purity was tested by flow cytometry assay. And K6lliker organ supporting cells were harvested to detect the rate and cycle of apoptosis by flow cytometry after Annexin V/PI staining, to test the cell growth curve by MTT assay, and to observe the differential expressions of the Bcl-2, Caspase-3, Caspase-8 and Caspase-9 through the Realtime PCR and Western blot. The calcium, potassium and glutamate concentrations in the culture medium of these cells in vitro were changed to detect the survival rate of cells by MTT assay.
RESULT:
The purity of K6lliker organ supporting cells by flow cytometry assay was 96. 56%. And these cells showed no significant difference in apoptosis, but an evident linear growth. The results of Realtime PCR and Western blot showed that the expression of Bcl-2, Caspase-3, Caspase-8 and Caspase-9 mRNA and protein in all different time points kept stable. Furthermore, the elevation of extracellular Ca2+ might contribute to decrease the cell viability of supporting cells. And K+ participated regulation of cell viability in a concentration-depending way. However, glutamate appeared to be a protective factor in high concentration.
CONCLUSION
There is no significant apoptosis in vitro of the supporting cells in the Kölliker organ of rats, showing a linear growth. The Ca2+ in high concentration might contribute to the apoptosis factor of these cells. However, the K+ and glutamate appear to be protective factors in high concentration.
Animals
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Animals, Newborn
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Apoptosis
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Caspase 3
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Cell Cycle
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Cell Proliferation
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Cell Survival
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Cochlea
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cytology
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growth & development
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Flow Cytometry
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In Vitro Techniques
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Rats
5.The Exploration of College Students'Internet Addiction
Chinese Journal of Medical Education Research 2005;0(06):-
When the Internet brings convenience to college students,it has deep influence on their behavior and mind.Internet Addiction Disorder(IAD)has drawn social attention widely.Therefore understanding the harm of IAD,finding out the causes and taking effective prevention and treatment measures are of positive significance to prevent college students' IAD.
6.A Probe into the Running of the Teaching Quality Supervision System in Institutions of Higher Learning
Chinese Journal of Medical Education Research 2006;0(12):-
The effective running of the teaching quality supervision system in institutions of higher learning is closely connected with the efficiency of the teaching quality supervision,thus affecting whether the teaching quality can be guaranteed and promoted or not.The paper,based on the formation of the teaching quality supervision system in institutions of higher learning,probes into the problem of how to effectively run the supervision for the purpose of promotion of teaching quality in colleges and universities.
7.Correlation between serum lipoprotein(a) level and carotid artery atherosclerosis in young and middle-aged patients with ischemic stroke: a retrospective case series study
International Journal of Cerebrovascular Diseases 2013;21(6):445-449
Objective To investigate the risk factors for carotid artery atherosclerosis in young and middle-aged patients with ischemic stroke and the effect of serum lipoprotcin(a) [(Lp (a)] level on young and middle-aged patients with ischemic stroke.Methods Patients with ischemic stroke between the ages of 18 to 55 were enrolled.Carotid artery ultrasonography was used to evaluate the degree of carotid artery atherosclerosis,and the concentrations of serum total cholesterol,triglycerides,high density lipoprotein cholesterol,low density lipoprotein cholesterol,very low density lipoprotein cholesterol,apolipoprotein A1,apolipoprotein B and Lp(a) were detected.According to the results of carotid ultrasound,the patients were divided into groups of without atherosclerosis,plaque without stenosis,and carotid artery stenosis.The demographic and clinical features were compared among the 3 groups.Multivariate logistic regression analysis was used to identify the independent risk factors for carotid artery atherosclerosis in young and middle-aged patients with ischemic stroke.Results A total of 106 patients with ischemic stroke (n =50 in the without atherosclerosis group,n =44 in the plaque without stenosis group,n =12 in the carotid artery stenosis group) were enrolled in the study.The constituent ratios of age (45.98 ±7.12,50.07 ±4.79,and 50.92 ± 1.83 years,respectively; F =7.169,P =0.001),hypertension (26.0%,47.7% and 58.3%,respectively; x2 =6.862,P =0.032),diabetes mellitus (22.0%,45.5% and 66.7%,respectively; x2 =10.729,P =0.005),hyperlipidemia (24.0%,40.1% and 75.0%,respectively; x2=11.372,P=0.003) and smoking (34.0%,61.4% and 75.0%,respectively;x2 =10.93,P=0.006),as well as the serum high-density lipoprotein cholesterol (1.03 ± 0.26,0.95 ± 0.26 and 0.76 ± 0.08 mmol/L,respectively; F=5.882,P =0.004) and Lp (a) (0.108 ± 0.044,0.155 ± 0.028 and 0.200 ± 0.011 g/L,respectively; F =41.556,P =0.000) levels had significant differences in the 3 groups.Multivariate logistic regression analysis showed that age > 48 years (plaques without stenosis:odds ratio [OR] 2.89,95% confidenee interval [CI] 1.20-6.96,P =0.018; carotid artery stenosis:OR 4.43,95% CI 1.19-16.57,P =0.027),hypertension (plaque without stenosis:OR 2.60,95 % CI 1.09-6.18,P =0.031; carotid artery stenosis:OR 3.99,95% CI 1.08-14.77,P =0.039),diabetes (plaque without stenosis:OR 2.96,95% CI 1.21-7.23,P=0.018; carotid artery stenosis:OR 7.09,95% CI 1.79-28.02,P =0.005),hyperlipidemia (plaque without stenosis:OR 2.19,95% CI 0.91-5.31,P =0.082; carotid artery stenosis:OR 9.50,95% CI 2.21-40.86,P =0.002),smoking (plaque without stenosis:OR 3.08,95% CI 1.33-7.16,P =0.009; carotid artery stenosis:OR 5.82,95% CI 1.39-24.38,P =0.016),and Lp (a) (plaque without stenosis:OR 4.38,95% CI 1.76-10.90,P=0.001; carotid artery stenosis:OR 12.80,95% CI 2.73-52.67,P =0.001) were the independent risk factors for carotid artery atherosclerosis in young and middle-aged patients with ischemic stroke.Conclusions Age,smoking hypertension,diabetes and Lp(a) were the independent risk factors for carotid artery atherosclerosis in young and middle-aged patients with ischemic stroke.
8.Stem cell transplantation for diabetic nephropathy:possibility, feasibility and application
Chinese Journal of Tissue Engineering Research 2014;(10):1635-1640
BACKGROUND:To control blood glucose, blood pressure, blood lipids and inhibit the rennin-angiotensin system is the main idea focused on the treatment of diabetic nephropathy, but the curative effect is unsatisfactory. Hemodialysis and kidney transplantation are suitable for serious cases, however, which is restricted because of the limited source of kidneys and high cost. Regenerative medicine research based on stem cells brings a new hope for treatment of diabetic nephropathy. OBJECTIVE:To comprehensively analyze the mechanism underlying different sources of stem cells for treatment of diabetic nephropathy and the clinical implications. METHODS:Papers addressing stem cells for the treatment of diabetic nephropathy were retrieved by computer in CNKI database and PubMed database from January 2005 to August 2013 with the key words“embryonic stem cells, bone marrow mesenchymal stem cells, induced pluripotent stem cells, diabetic nephropathyin Chinese and English. Papers published recently or in journals with high impact factor were selected. A total of 60 papers were included for review. RESULTS AND CONCLUSION:Embryonic stem cells, bone marrow mesenchymal stem cells, induced pluripotent stem cells have the potential to differentiate into renal histiocytes. A large numbers of experimental studies have shown that stem cells transplantation has a positive effect on recovery of injured kidney. Stem celltransplantation can provide a novel therapy for diabetic nephropathy.
9.Evaluation of cardiovascular function in children with septic shock
Journal of Clinical Pediatrics 2010;(1):7-9
Carefully evaluation of cardiovascular function in children with septic shock is the basis of appropriate therapy.Clinical methods of cardiovascular function evaluation include clinical manifestation,hemodynamic momtoring and laboratory test of tissue oxygenation.Evaluation of cardiovascular function should combine all results from different methods and should be dynamically monitored with the development of septic shock.
10.A randomized controlled clinical trial of acupuncture to prevent nausea and vomitingafter breast surgery
The Journal of Practical Medicine 2015;31(23):3950-3952
Objective To investigate whether acupuncture can prevent postoperative nauseaand vomiting (PONV)in patients received breast surgery. Methods Two hundred and forty patients were randomly divided into the acupuncture group and the control group. Patients in the acupuncture group were treated at bilateral Hegu (L4) and Neiguan (P6) after surgery. Patients in the control group received no any interventions. The occurrence of postoperative nausea and vomiting, the number of complete remission and the remedial treatment were record ed. Results The incidences of nausea and vomiting in the acupuncture group were lower than those in the control group from 2 h to 6 h post-operation, with less need of remedy treatment (P < 0.05, respectively). Conclusion Acupuncture in preventing nausea and vomiting after breast surgery owns a certain value in the early postoperative (6 h) stage, with effectively reducing the occurrence of postoperative nausea and vomiting.