Reverse transcription loop-mediated isothermal amplification(RT-LAMP)is a novel nucleic acid amplification technique which has been mainly used in a variety of viruses and other pathogens of gene detection.Compared with reverse transcriptase polymerase chain reaction (RT-PCR),the RT-LAMP has advantages of reaction simplicity,rapidly,cost-effective,detection sensitivity and specificity.Nowadays,RT-LAMP technology is gradually used in various medical fields,but it is still a new method especially in cancer diagnosis of tumor gene.The article reviews the application of the RT-LAMP technology in the cancer

Researches indicate that gastric carcinoma occurs where gastric stem cells settle down.Stem cells may play an important role in formative process of gastric carcinoma. Researches on relationship between stem cells and gastric stem cells, tumor stem cells and stomach neoplasms, bone marrow stem cells and stomach neoplasms indicate that gastric carcinoma may be a kind of stem cell diseases.

Objective To explore the correlation between serum HBV-DNA content and hepatic fibrosis indicators in hchronic epatitis B patients.Methods Serum HBV-DNA content was determined by using real-time fluorescence quantitative PCR and he-patic fibrosis indicators including hyaluronic acid,laminin,procollagen Ⅲ,and collagen Ⅳ which were determined by using chemilu-minescence.Results The logarithm value of serum HBV-DNA content of the 110 hepatitis B patients was 5.32±1.37.Serum hyal-uronic acid concentration was (197.81 ±85.37)mg/mL;procollagen Ⅲ concentration was (142.66 ±30.28)μg/mL.Collagen Ⅳconcentration was (90.34 ±20.53)μg/mL.Laminin was (143.96 ±31.79 )μg/mL.All these indicators were significantly higher than the control group (P <0.01).But there were no significant linear correlation between serum HBV-DNA content and the four hepatic fibrosis indicators(P >0.05).Conclusion The hepatic fibrosis indicators increased significantly in hepatitis B patients but there were no significant correlations between serum HBV-DNA content and the hepatic fibrosis indicators.

Objective To investigate the effects of Astragalus membranaceus and Angelica sinensis on the hemodynamics and pathological features of arterial wall in restenosis of blood vessel in rats in order to find the potential mechanism of the effect of Astragalus membranaceus and Angelica sinensis in preventing restenosis after ablation of intima. Method Thirty SD rats were randomly divided into 5 groups (6 each):control group (C),model group (M),Astragalus membranaceus treatment group (H),Angelica sinensis treatment group (D),and combined Astragalus membranaceus with Angelica sinensis treatment group (HD). The restenosis model was reproduced by denuding rat's aorta endothelium with balloon catheter in all the groups except group C. After intramuscular injection of Astragalus membranaceus 0.42ml/(kg?d) in group H,Angelica sinensis 0.21ml/(kg?d) in group D,and Astragalus membranaceus 0.42ml/(kg?d) together with Angelica sinensis 0.21ml/(kg?d) in group HD for 21 days,the changes in hemodynamics and the pathological features of arterial wall were observed in all the groups. Result Compared with rats of group C,in which the intima was smooth and the smooth muscle cells (VSMCs) in media were orderly arranged,the intimal thickness of aorta,pulsatile index (PI) and resistance index (RI) increased,while the diameter of lumen,blood velocity decreased remarkably in group M. Compared with the rats in group M,the extent of hyperplasia and thickness was notably reduced,RI and PI were lower while blood velocity was lowered in group H,D and HD after being treated for 21 days. Conclusion Hyperplasia induced by remaval of endothelial cells may be reduced and blood circulation may be improved to different extents by individually or simultaneously use of Astragalus membranaceus and Angelica sinensis,thus preventing restenosis of the aorta after removal of its intima.

Objective To observe the dynamic expression and location of TGF-β1 and TGF-β receptor Ⅰ ,Ⅱ at different time in an experimental model of rat molars reversible pulpitis, to evaluate the role of TGF-β1 and TGFβ receptor Ⅰ , Ⅱ on pulp injury and repair. Methods The reversible pulpitis animal model was established by drilling the enamel without water cooling and then acid etching dentine. The maxillary and teeth of each animal were made 5μm thick serial slides. The slides were stained with hematoxylin-eosin and SABC immunehistochemical technique and then studied under the light microscope and under image analysis, all the data were statistically analyzed by analysis of variance (ANOVA) and t-test. Results Positive staining of TGF-β1 and TβR Ⅰ ,TβR Ⅱ was found in odontoblasts, pulp fibroblasts and vascular endothelial cells. In pulpitis stage,the expressions of TGF-β1 and TGF-β receptor Ⅰ , Ⅱ were increased markedly, and showed dynamic changes at different time;TGF-β1 show a peak at 5d and followed by decreased significantly. TGF-β receptor Ⅰ ,Ⅱshowed the highest at 3d ,and then decreased slightly. From 0d to 3 d,TGF-β1 and TGF-β receptorⅠ,Ⅱ showed uptrends, but their uptrends had no direct proportion relationship. Conclusion The results presented here suggested that TGF-β1 and TGF-β receptorⅠ,Ⅱ might play an important role in pulp wound and repair. The inflammatory reaction in response to TGF-β1 was mediated by TGF-β receptorⅠ, Ⅱ; Although TGF-β1 showed a possible function for the TGF-β receptor,but a direct proportion relationship couldn't be concluded.

Useful pharmacodynamic changes occur when some Chinese medicine are used together with some Western medicine, namely enhanced curative effect, lowered adverse reactions, reduced dosages, shortened treatment courses, enlarged indications scope, improved compliance of treatment and rational medication, which could be explored to provide scientific bases for further improving diagnosis and treatment levels and rational use of drugs.
Drugs, Chinese Herbal ; metabolism ; Humans ; Medicine, East Asian Traditional ; Pharmaceutical Preparations ; metabolism ; Pharmacokinetics ; Phytotherapy

ObjectiveTo explore the roles of Toll-like receptor (TLR) 3,7 and 9 in the pathogenesis of pityriasis rosea.MethodsThis study recruited 40 patients with pityriasis rosea and 40 normal human controls.Reverse transcription-PCR was carried out to detect the expressions of TLR3,7 and 9 in peripheral blood lymphocytes of these subjects.ResultsThe mRNA expressions of TLR3,7 and 9 in peripheral blood lymphocytes were significantly higher in patients with pityriasis rosea than in the normal human controls (Z =4.239,3.745,2.658,all P ＜ 0.05).There was a positive correlation between the expression of TLR3 and TLR7(r =0.574,P ＜ 0.01 ) as well as between that of TLR3 and TLR9 (r =0.551,P ＜ 0.01 ) in the patients,while there was no correlation between TLR7 and TLR9 expression in the patients or between the expressions of TLR3,TLR7 and TLR9 in the controls.ConclusionVirus infection might be involved in the pathogenesis of pityriasis rosea via TLR pathways.

Homebox genes are known to determine cell proliferation and differentiation and subsequently regulate the occurrence,development and prognosis of many tyhpes of malignant tumors in human.Some recent studies have shown that homebox genes are abnormally expressed in the esophageal,gastric,coloreetal and other gastrointestinal tumors.In this review,we consider that homebox genes may regulate the occurrence,development of gastrointestinal tumors by interacting with transcription factors in vivo and abnormal epigenetic modifications.Therefore,homebox genes are closely related to gastrointestinal tumors.

Chemotherapy resistance is a great obstacle for effective treatment of ovarian cancer.Drug resistance of ovarian cancer is the results of combined action of multi- factors.Further study of drug resistancerelated genes can provide new ideas for reversing drug resistance fundamentally.

Objective To investigate the expressions of SOCS3 and IL‐6 in triple‐negative breast cancer(TNBC)and their clinical significance.Methods The expressions of SOCS3 and IL‐6 in TNBC and normal breast cells were detected by Western blot and fluorescence quantitative PCR.Immunohistochemical staining was used to detect their expressions in 84 TNBCs ,33 breast fibromas and 11 normal breast tissues. The correlation of their expressions in TNBC and the relationship between their expressions and clinical pathological parameters were analyzed as well.Results SOCS3 was not expressed or down‐expressed in TNBC cell lines while IL‐6 was over‐expressed. The positive rate of SOCS3 protein was 32.1% in TNBCs ,63.6% in breast fi‐bromas(P<0.01)and 72.7% in normal breast tissues (P< 0.01);and that of IL‐6 protein was 72.6% in TNBCs ,30.3% in breast fibromas(P< 0.01)and 27.3% in normal breast tissues (P< 0.01).Moreover ,a significant negative correlation was found between the expression of SOCS3 and that of IL‐6 in TNBC(rs = -0.590 ,P<0.01). The expressions of SOCS3 and IL‐6 were markedly associated with tumor size ,lymph node metastasis ,clinical stages and expressions of Ki‐67 and p53(P<0.05) , but not with age ,histological grades or menopausal status(P>0.05).Conclusion The expressions of SOCS3 and IL‐6 are asso‐ciated with the development of TNBC ,suggesting that SOCS3 and IL‐6 can serve as biological markers for evaluating the prog‐nosis ,and therapeutic targets of T NBC.