Objective:To explore the dynamic expression and migration of the novel testis- and sperm-specific protein FSCB in mouse spermatogenesis and observe the morphological localization of FSCB on normal and abnormal mouse sperm. Methods: For exactly staging of the mouse tubule sections, Periodic acid-Schiff staining was performed at first, and then the dynamic expression of FSCB on mouse testis and sperm by indirect Immunofluorescence was observed. Results:FSCB is a post meiotic protein initially expressed at step 11(stage Ⅺ)of mouse spermatogenesis in the elongating spermatids, and it’s expresson gradully become stronger and stronger from step 11(stage Ⅺ) through step 15 (stage Ⅵ). It subsequently begin migrate into the flagellar at step 15, and at last totally localize to the sperm flagellar at step 16(stage Ⅷ)accompanied with its disappearing from the cytoplasma of sperm. The exact localization of FSCB on normal sperm is in the flagellar principal piece. Its expression and localization on abnormal sperm was variablely changed:a.No expression on the whole flagellar;b. The expression segment on the flagellar was shift to near the head and become short, suggest this sperm defect middle piece;c. Its distribution on the flagellar showed beads like shape. Conclusion: FSCB is an important constituent protein of the fibrous sheath, is assembled at the surface of fibrous sheath core structure and constitutes the final assembly step of fibrous sheath at the later stages of spermiogenesis. The expression of FSCB in abnormal sperm was significantly changed. The relationship between the abnormal expression of FSCB and the sperm motility need to be further studied.

With the rapid development at home and abroad,mobile learning has become the fo-cus in the education field. In response to the current medical English teaching situation, this paper ana-lyzed the prospects that mobile learning can promote medical English teaching from the perspectives of improving students' learning interests,creating collaborative learning environment and enhancing immer-sion learning experiences and drew the conclusion that mobile learning can enlighten medical English teaching from aspects of changing teaching philosophy,updating teaching mode and training teachers.

Objective To evaluate diagnosis value of PP65 antigenaemia assay(AA) on cytomegalovirus(CMV) infection in febrile children.Method One hundred and twenty-eight febrile children with febrile duration between one to two weeks,their blood preparations and urine aliquots were screened,indirect IF staining(antigen PP65),ELISA(CMV-IgM) and light microscope(urinary cytomegalic inclusion).Three methods above mentioned were used in all patients.Result The positive rate of PP65 antigenaemia,CMV-IgM and urinary cytomegalic inclusion was 17.2%,8.6%,6.3%,respectively.The positive coincidence rate of PP65 antigen with CMV-IgM and PP65 antigen with urinary cytomegalic inclusion was 50%(11/22),36%(8/22),respectively.Nineteen childrens antigenaemia PP65 became negative with patient′s condition improved.Three children′s PP65 antigenaemia remained positive,when they were clinic cured.Two of them became negative after two weeks and one after three weeks.Conclusion Antigenaemia PP65 is effective in early diagnosis of active CMV infection,predicting patient′s condition and providing early intervention and treatment.

2500mg/kg ( ig ) gave no any toxic symptom nor death. In long-term toxicity test, 50, 1000 and 2000mg/kg?d-1 ig on 30~90 consecutive days to rats and 280 and 560mg/kg?d-1 ig on 90 consecutive days to dogs respectively, revealed no significant toxicity in general physical signs, body weight, appearance. behavious, routine urine test, routine blood test, liver and kidney function tests, histological examinations of important organs, blood biochemistry tests and electrocardiogram, except that the platelet numbers of both animals increased.

Objective To observe the effect of Pingyi(PY) Mixture combined with chemotherapy to Ⅱ～Ⅲ patients after tumour surgery which can resist transference and recurrence of tumour.Method According to the random principle,120 patients who had suffered the surgery of commonly seen tumour were divided into observed group and matched group.Observed group adopted PY Mixture combined with chemotherapy and matched group adopted simple chemotherapy.After 3 periodic treatments,the effect of the patients after tumour surgery was observed including the ratio of recurrence and transference of observed group within 1 or 2 years,survival ratio,quality of life,body weight,immunological indexes,the toxic and sub-effect,the blood rheology behavior,and compared with simple chemotherapy.Results The ratio of recurrence and transference of observed group within 1 or 2 years was respectively 25.74% and 31.43%,which was obviously lower than that of control group,which was 32.00%,52.00%(P

Objective To construct a finite element analytical model of multiloop edgewise arch wire(MEAW),and study its mechanical characteristics.Methods Three-dimensional(3D) model of dentition-bracket-arch wire was set up in an initial graphics exchange specification(IGES) format complex by Pro/E software.The multiloop edgewise arch wire was spliced on the lower dentition.After dividing the arch wire and brackets with quarter-tetrahedron element method,the model was analyzed by ANSYS(an engineering simulation software).Results The finite element analytical model of multiloop edgewise arch wire was successfully built up.Based on the model,when multiloop edgewise arch wire was subjected to a heavy strength in certain parts(such as tooth 3,4),the anti-force strength on the other brackets(such as tooth 1,2,5,6,7) was not so heavy.The subjected strength was light and soft,especially on the first molar.Conclusion The results suggest that the finite element analytical model of multiloop edgewise arch wire built up in the experiment may be used to study the integrated mechanical characteristics,reveal the orthodontic specialty,and discuss the orthodontic mechanisms of multiloop edgewise arch wire techniques.

Objective To study the differences between diet induced obesity (DIO) and diet induced obesity resistance (DIO-R) during insulin resistance (IR) in rats. Method Male SD rats were fed by high-fat diet 1 (45 % fat kJ) after adaptation. At 3rd week, rats were divided into 3 groups according to their body weight: rats belonging to the middle 1/3 of their body weights were the control group, and was fed by control diet (10 % fat kJ). The heaviest 1/3 and the lightest 1/3 belong to DIO and DIO-R group, respectively, and were fed by high-fat diet 2 (60 % fat kJ). Five rats were selected randomly from each group and sacarified at 3rd, 6th, 8th, 10th and 11th week. The energy intake, body fat contents, fasting blood glucose (FBG), serum lipid, serum insulin, insulin sensitive index(ISI)and leptin of rats were monitored dynamically. Results DIO rats were significantly different from DIO-R and control rats in energy intake, body fat content, FBG, lipid, serum insulin and leptin. Conclusion Fed by the same high-fat diet, DIO rats are more sensitive than DIO-R rats to become IR.

OBJECTIVE To observe the teratogenecity of ethylene oxide sterilized materials to cells which are used for biotherapy.METHODS Three kinds of cells were cultivated in ethylene oxide sterilized culture flasks which were conserved for different time and high temperature/high pressure sterilized vitric culture flasks.NK/CIK cells induced from cord blood,human mesenchymal stem cell and CD34+ cells were cultivated in ethylene oxide sterilized culture flasks which were conserved for one day(group A),for three months(group B),for more than six months(group C) and cultivated in high temperature/high pressure sterilized vitric culture flasks(group D).After ten days,not only the growth and morphology of cells but also the breakages fragmentations and gaps in chromosomes and replication of chromosome in nucleus were observed.According to these data,the malformation rate was calculated.RESULTS Compared with groups C and D,the inductivity of groups A and B obviously degraded(P0.05).CONCLUSIONS Compared with the control group,the materials which were sterilized by ethylene oxide and conserved for less than one day and three months can bring damage to cells.However,the damage is not discovered in the materials which were conserved for more than six months.Ethylene oxide can induce cell aberrant and mutation.Therefore,it is suggests that materials be conserved for more than six months to ensure safe and effective enforcement of biotherapy.

Traditional sequences of magnetic resonance imaging (MRI) such as T1-weighed imaging, T2-weighed imaging, and fluid-attenuated inversion recovery (FLAIR) imaging can sensitively detect the subacute and chronic intracranial hemorrhage; gradient echo sequence can detect various stages of intracranial hemorrhage, but there is no correlation between the signal intensity of the hematoma and the time of the presence of hematoma. Diffusion-weighted imaging and apparent diffusion coefficient may provide more information about the center and surrounding areas of hematoma, as well as the hemorrhagic tendency after infarction.

Objective To study the expression of PTEN et al and their significance in T lymphoblastic lymphoma/leukaemia (T-LBL/ALL). Methods Seventy-six cases of T-LBL/ALL were studied by using immunohistochemistry (EnVision method) for CD3, CD7, CD10, CD20, CD23, CD43, CD45RO, CD99, TdT, MPO and PTEN. Follow-up was included. Results In the Seventy-six cases of T-LBL/ALL, the percentages of tumor cells expressing TdT, CD99, CD3, CD7, CD45RO and CD43 were 93.42%, 94.74%, 67.12%, 92.11%, 36.85%,and 51.33%, respectivly while MPO, CD20 and CD23 were all negative. The index of Ki-67 expression higher than 80 % was found in 27 and ≤80% in 49 cases. The expression of PTEN (64.47 %) in T-LBL/ALL was lower than that of in reactivated lymphoid tissue (100%, P<0.05). PTEN was reversely correlated with stage,Ki-67 expression and LDH level (P<0.05). CD3-positive cases were more than CD3-negative cases in abnormal LDH level (P<0.05). Follow-up data was obtained in 50 cases, ranging from 1 to 108 months. The overall survival rate was 35.8 %, with median survival time 330 days. No prognostic significance was found in the expression of PTEN, CD3, CD7, CD10, CD43, Ki-67 and CD45RO (P >0.05). Conclusion The antibodies of CD3,CD7, CD10, CD20, CD43, CD45RO, CD99, TdT, MPO and Ki-67 were very helpful for the diagnosis of T-LBL/ALL.Down-regulation of PTEN may play an important role on the development of T-LBL/ALL.