Objective To develop a blooding device for blood sucking insects. It's designed for researches, such as artificial breeding of indoor medium insects, evaluation of vermicides'effect, and artificial infection of pathogenic microorganisms. Methods This device was developed with AT89C51 MCU. Results The desired instrument could provide blood which could be adjusted from 30℃to 38℃. The error of temperature could be controlled within 0.5℃. Conclusion The instrument is easily-operated and stable. It can replace the artificial blood-breeding.

Ambulatory blood pressure monitor (ABPM) was done on 103 hypertensive patients according to clinical stages and left ventricular measure (LVM) was randomly done on a portion of the patients. The results showed that the variability of stage-Ⅱ-group's 24 hour systolic pressure and stage-Ⅲ-group's systolic and diastolic pressures was significantly higher than that of stage-Ⅰ-group (P

Objective To determine the content of Ginsenoside Rb1 by RP-HPLC. Methods HPLC was employed with a column of Diamonsil ODS-C18 and a mobile phase of Acetonitrile-H2O (29∶71), the detection wavelength at 203 nm and the column temperature at 25 ℃. Results The linear correlation is available between 0.776 and 7.760 ?g/mL of Ginsenoside Rb1, and the correlation coefficient was 0.999 99, the average recovery was 99.69% and RSD was 2.04% (n=6). Conclusion The method is simple and accurate, and applicable to control the quality of Chanshu Granule.

[Objective]To discuss the clinical results of reconstruction of block bone deficit knee(lower femur and upper tibia) by transplantation of patella and present surgery protocols.[Methods]There were 7 patients who have giant cell tumor of bone in the lower femur or upper tibia at the knee in this report.The case history was from 4 to 12 months,average 5.8 months.Four were male and three were female.The age ranged from 18 to 40 years,average 31.8 years.Four had lesions at the lower femur and the other three had lesions at the upper tibia.According to Ennecking grade,five cases were in grade 2 and two cases were in grade 3.When the cartilage of the knee was damaged by bone tumors,the author supplemented it by autogenous patella to fill the defect.[Results]All cases of patella grafts were presented,with follow-up from 3 to 10 years(average 4.9 years),three cases were followed up more than 5 years.During the follow up,there was no case with recurrence、metastasis or necrosis and infection of graft.All the grafts had good union.According to the function evaluation standard of the international protecting extremities institute,3 excellent,4 good.The main complications were less range of movement of the knee(movement range 40～110 degree,average 88 degree).Weakness of the extensor apparatus was not obvious.One case had slight collapse of tibia plateau and another case sufferd from joint pain 6 years after operation.[Conclusion]When the cartilage of the lower femur and the upper tibia is damaged by bone tumors,the bone tumors and its affected condyle cowld be incised,then apply patella grafts to repair and reconstruct the defect of the knee.

Objective To investigate the feasibility and clinical effect of hip joint arthrodesis with the application of Kirschner wire and reconstruction plate in the treatment of advanced tuberculosis in total hip joint.Methods A total of 30 patients with advanced tuberculosis of the hip in our hospital from March 2014 to April 2016 were divided as Kirschner wire group and reconstruction plate according to different surgical methods,with 15 cases in each group.The operation time,bleeding volume during operation (intraoperative and postoperative),fusion time,postoperative complications of two groups were observed and compared.Results Patients of two groups reached bony fusion.Compared with reconstruction plate group,Kirschner wire group had less blood loss,shorter operative time,longer fusion time,the differences were statistically significant (P ＜ 0.05).Conclusion Both Kirschner wire fixation and reconstruction plate arthrodesis are effective for patients with advanced tuberculosis of total hip joint,but the clinical efficacy of Kirschner wire fixation in hip arthrodesis was significantly better than the reconstruction plate.

Objective:To compare the oncology effect and risk between hepatopplenectomy and conventional hepatectomy for hepatocellular carcinoma patients with hypersplenism, so as to provide guidance for clinical application.Methods:One hundred and eighteen patients with hepatic carcinoma with hypersplenism in the Private hospitals of Zhejiang Province Yiwu were studied retrospectively. Patients were divided into 2 groups according to different operation methods: combined hepatosplenectomy group and conventional hepatectomy group with 59 cases in each group. Blood routine, liver function, complication and tumor effect (local recurrence, distant metastasis and 1, 3, 5 years survival rate) was compared between two group.Results:There was no significant difference in operation time, intraoperative blood loss and hospitalization time between the two groups ( P>0.05). Compared with that of the control group, the clinical total effective rate of the combined group was significantly increased: 94.92% (56/59) vs. 79.66% (47/59), P<0.05; there was no significant difference in the incidence of complications between the two groups ( P>0.05); the two groups were followed up to August 1, 2019. The median survival time of the two groups was significantly higher than that of the open group (29 months vs 22 months) ( P<0.05); the local recurrence rate, distant metastasis rate, 1, 3 and 5-year survival rate between the two groups were statistically significant ( P<0.05). The platelet and white blood cells in the combined group were significantly higher than those in the control group after 7 days′ treatment: (264.48 ± 75.37) × 10 9/L vs. (153.53 ± 42.11) × 10 9/L, (7.86 ± 1.43) × 10 9/L vs. (3.48 ± 1.32) × 10 9/L, P<0.05. Compared with that of the control group, alanine aminotransferase and aspartate aminotransferase were significantly decreased: (49.42 ± 31.88) U/L vs. (84.22 ± 43.95) U/L, (36.50 ± 21.50) U/L vs. (49.98 ± 35.63) U/L, P<0.05. Conclusions:Compared with conventional hepatectomy for hepatocellular carcinoma patients with hypersplenism, combined hepatosplenectomy can effectively promote the increase of peripheral blood PLT and WBC and the recovery of liver function without increasing the risk of surgery, and the long-term follow-up results are more satisfactory, which has high clinical application value.

Object To investigate the berberine in Coptis chinensis Franch. processed with various quantity of Evodia rutaecarpa (Juss.) Benth. and the components absorbed from E. rutaecarpa. Methods Taking berberine, evodiamine and rutaecarpine as targets, HPLC method was used to determine the components of C. chinensis, E. rutaecarpa, C. chinensis processed with 10%, 20%, 30%, 40%, 50% E. rutaecarpa. Results The content of berberine in C. chinensis processed with E. rutaecarpa decreased, and that of C. chinensis processed with 20% and 30% E. rutaecarpa was higher than the rest. C. chinensis processed with E. rutaecarpa absorbed the components of E. rutaecarpa really. Conclusion The suitable quantity of E. rutaecarpa is 20% when processing for C. chinensis.

Aim To explore the hemodynamic change of elderly isolated hypertension(EISH). Methods Color doppler ultrasonography were applied to examine thirty EISH patients and twenty eight elderly healthy subjects. The left ventricular systolic function as well as hemodynamic parameters were measured. Results The indices of left ventricular systolic function in EISH group such as SV,CO,EF and CI were obviously higher than those of control group(P<0.05),Linear relevant analysis show that pulse pressure was remarkable related to SV,CO,EF and CI in EISH group,with r value 0.43,0.56,0.46,0.45 respectively.Conclusion EISH patients apparently had ventricular systolic dysfunction and hemodynamic abnormality. Pulse pressure may be associated with development of sysytolic dysfunction.

Objective:To improve the quality standard for Kangshiming mixtures. Methods:The identification of Angelicae Sinen-sis Radix, Astragali Radix and Phellodendri Chinensis Cortex was carried out by TLC. The contents of puerarin and paeoniflorin in the preparations were determined by HPLC. Results:The spots displayed in TLC were clear without interference from the negative control. The linear range for puerarin was 1. 64-49. 20μg·ml-1(r=0. 999 9) and 4. 22-63. 30μg·ml-1(r=0. 999 6) for paeoniflorin. The average recovery was 99. 63%(RSD=2. 14%,n=9) and 99. 05%(RSD=2. 70%,n=9) for puerarin and paeoniflorin, respectively. Conclusion:The method is accurate, reliable and specific, and can be used in the quality control of Kangshiming mixtures.

Objective To detect the variations of the serum α-MSH and TNF-α in multiple-trauma patients and discuss their role in severity of casualties.Methods Fifty casualties were divided into two groups for study.There were 30 casualties with moderate severe trauma(ISS 16 ～ 25 point)and 20 patients with extreme severe trauma(ISS ＞ 25 point),and another 15 healthy subjects were enrolled as controls.The blood samples were obtained within 24 hours,and 3 days,5 days,7 days after admission.The serum levels of α-MSH and TNF-α in casualties with multiple injuries were determined by using enzyme-linked immunosorbent double antibody sandwich method(ELISA).The data were expressed in((x)± s),and analyzed with chi-square test and repetitive measures of ANOVA by using SPSS 13.0 package.P value less than 0.05 indicated statistical significance Results The serum α-MSH levels of casualties within 24 hours,and 3 days,5 days,7 days after injury in the two groups were much lower than those in the control group (P ＜ 0.01),while the serum TNF-α levels of casualties were much higher than those in the control group (P ＜0.01).The serum α-MSH levels of casualties with extreme severe traumawere lower,and the TNF-αlevels of casualties with extreme severe trauma were higher than those in patients with moderate severe trauma(P ＜0.01,respectively).There were negative correlations between two biomarkers 24 hours,5d and 7d after injury.Conclusions In casualties,the serum levels of α-MSH decreased and the serum levelsof TNF-α increased,and the degrees of changes were closely depended on the severity of trauma,the more severe the more significant changes.There was a negative correlation between two biomarkers.