Objective:To explore the dynamic expression and migration of the novel testis- and sperm-specific protein FSCB in mouse spermatogenesis and observe the morphological localization of FSCB on normal and abnormal mouse sperm. Methods: For exactly staging of the mouse tubule sections, Periodic acid-Schiff staining was performed at first, and then the dynamic expression of FSCB on mouse testis and sperm by indirect Immunofluorescence was observed. Results:FSCB is a post meiotic protein initially expressed at step 11(stage Ⅺ)of mouse spermatogenesis in the elongating spermatids, and it’s expresson gradully become stronger and stronger from step 11(stage Ⅺ) through step 15 (stage Ⅵ). It subsequently begin migrate into the flagellar at step 15, and at last totally localize to the sperm flagellar at step 16(stage Ⅷ)accompanied with its disappearing from the cytoplasma of sperm. The exact localization of FSCB on normal sperm is in the flagellar principal piece. Its expression and localization on abnormal sperm was variablely changed:a.No expression on the whole flagellar;b. The expression segment on the flagellar was shift to near the head and become short, suggest this sperm defect middle piece;c. Its distribution on the flagellar showed beads like shape. Conclusion: FSCB is an important constituent protein of the fibrous sheath, is assembled at the surface of fibrous sheath core structure and constitutes the final assembly step of fibrous sheath at the later stages of spermiogenesis. The expression of FSCB in abnormal sperm was significantly changed. The relationship between the abnormal expression of FSCB and the sperm motility need to be further studied.

With the rapid development at home and abroad,mobile learning has become the fo-cus in the education field. In response to the current medical English teaching situation, this paper ana-lyzed the prospects that mobile learning can promote medical English teaching from the perspectives of improving students' learning interests,creating collaborative learning environment and enhancing immer-sion learning experiences and drew the conclusion that mobile learning can enlighten medical English teaching from aspects of changing teaching philosophy,updating teaching mode and training teachers.

Objective To evaluate diagnosis value of PP65 antigenaemia assay(AA) on cytomegalovirus(CMV) infection in febrile children.Method One hundred and twenty-eight febrile children with febrile duration between one to two weeks,their blood preparations and urine aliquots were screened,indirect IF staining(antigen PP65),ELISA(CMV-IgM) and light microscope(urinary cytomegalic inclusion).Three methods above mentioned were used in all patients.Result The positive rate of PP65 antigenaemia,CMV-IgM and urinary cytomegalic inclusion was 17.2%,8.6%,6.3%,respectively.The positive coincidence rate of PP65 antigen with CMV-IgM and PP65 antigen with urinary cytomegalic inclusion was 50%(11/22),36%(8/22),respectively.Nineteen childrens antigenaemia PP65 became negative with patient′s condition improved.Three children′s PP65 antigenaemia remained positive,when they were clinic cured.Two of them became negative after two weeks and one after three weeks.Conclusion Antigenaemia PP65 is effective in early diagnosis of active CMV infection,predicting patient′s condition and providing early intervention and treatment.

2500mg/kg ( ig ) gave no any toxic symptom nor death. In long-term toxicity test, 50, 1000 and 2000mg/kg?d-1 ig on 30~90 consecutive days to rats and 280 and 560mg/kg?d-1 ig on 90 consecutive days to dogs respectively, revealed no significant toxicity in general physical signs, body weight, appearance. behavious, routine urine test, routine blood test, liver and kidney function tests, histological examinations of important organs, blood biochemistry tests and electrocardiogram, except that the platelet numbers of both animals increased.

The development of gastrointestinal minimally invasive surgery in China is changing during the past decade.The Chinese laparoscopic gastrointestinal surgery study (CLASS) group as the earliest and most closely cooperated organization for surgical clinical researches,under the support of Chinese Medical Association,Chinese Anti-cancer Association and Chinese Medical Doctor Association,the clinical research capacity and minimally invasive technology of the CLASS group continue to be developed.With the launch of the prospective,randomized,controlled CLASS-01 trial for the treatment of locally advanced gastric cancer in 2012 and the publication of safety results of laparoscopic surgery for gastric cancer in 2016,the international academic community is increasingly focusing its attention on China.This article attempts to review the history and development of the CLASS group in the past 7 years,aiming to prospect the future and attempt to create trends.

Objective To investigate the prevalence of lower urinary tract symptoms in women in a community in Fuzhou. Methods We designed a patient-completed questionnaire according to Bristol Female Lower Urinary Tract Symptoms (BFLUTS) questionnaire.A few parts of the questionnaire were adjusted based on living habits and social backgrounds in our country.The health care workers distributed and collected the questionnaires and instructed the women to fill in them one by one. Results A total of 6066 questionnaires were distributed and 5587 were collected. Of the responders 4745 women were more than 18 years. The prevalence of stress incontinence, urgency incontinence,urgency,frequency and nocturia were 9.0% (425/4745),2.4% (113/4745),10.3% (489/4745),1.5% (72/4745) and 9.8% (446/4745),respectively.The prevalence of storage symptoms increased with age,showing a 20.5%(104/507) in 18~27 years’ group,19.1% (332/1740) in 28~37,22.5%(338/1503) in 38~47,32.2%(202/628) in 48~57,41.2%(61/148) in 58~64,58.0% (127/219) in ≥65 years’ group. The overall prevalence was 24.5%.The prevalence of delayed urinary,intermittent stream,increased abdominal urination, incomplete emptying and voiding pain were 7.0%(330 cases),4.1%(194),4.6% (217),4.9% (233) and 4.8%(229),respectively.The prevalence of general voiding dysfunction increased with age,showing a 13.0% (66/507) in 18~27 years’ group,9.3% (162/1740) in 28~37,10.7%(161/1503) in 38~47,13.2%(83/628) in 48~57,16.2% (24/148) in 58~64 and 26.9%(59/219) in ≥65 years’ group.The overall prevalence was 11.7%. Conclusions The prevalence of lower urinary tract symptoms are high in community-dwelling women,and the prevalence of storage symptoms are more common than that of voiding dysfunction in these women.

Objective To construct a finite element analytical model of multiloop edgewise arch wire(MEAW),and study its mechanical characteristics.Methods Three-dimensional(3D) model of dentition-bracket-arch wire was set up in an initial graphics exchange specification(IGES) format complex by Pro/E software.The multiloop edgewise arch wire was spliced on the lower dentition.After dividing the arch wire and brackets with quarter-tetrahedron element method,the model was analyzed by ANSYS(an engineering simulation software).Results The finite element analytical model of multiloop edgewise arch wire was successfully built up.Based on the model,when multiloop edgewise arch wire was subjected to a heavy strength in certain parts(such as tooth 3,4),the anti-force strength on the other brackets(such as tooth 1,2,5,6,7) was not so heavy.The subjected strength was light and soft,especially on the first molar.Conclusion The results suggest that the finite element analytical model of multiloop edgewise arch wire built up in the experiment may be used to study the integrated mechanical characteristics,reveal the orthodontic specialty,and discuss the orthodontic mechanisms of multiloop edgewise arch wire techniques.

Objective To study the differences between diet induced obesity (DIO) and diet induced obesity resistance (DIO-R) during insulin resistance (IR) in rats. Method Male SD rats were fed by high-fat diet 1 (45 % fat kJ) after adaptation. At 3rd week, rats were divided into 3 groups according to their body weight: rats belonging to the middle 1/3 of their body weights were the control group, and was fed by control diet (10 % fat kJ). The heaviest 1/3 and the lightest 1/3 belong to DIO and DIO-R group, respectively, and were fed by high-fat diet 2 (60 % fat kJ). Five rats were selected randomly from each group and sacarified at 3rd, 6th, 8th, 10th and 11th week. The energy intake, body fat contents, fasting blood glucose (FBG), serum lipid, serum insulin, insulin sensitive index(ISI)and leptin of rats were monitored dynamically. Results DIO rats were significantly different from DIO-R and control rats in energy intake, body fat content, FBG, lipid, serum insulin and leptin. Conclusion Fed by the same high-fat diet, DIO rats are more sensitive than DIO-R rats to become IR.

Objective To observe the effect of Pingyi(PY) Mixture combined with chemotherapy to Ⅱ～Ⅲ patients after tumour surgery which can resist transference and recurrence of tumour.Method According to the random principle,120 patients who had suffered the surgery of commonly seen tumour were divided into observed group and matched group.Observed group adopted PY Mixture combined with chemotherapy and matched group adopted simple chemotherapy.After 3 periodic treatments,the effect of the patients after tumour surgery was observed including the ratio of recurrence and transference of observed group within 1 or 2 years,survival ratio,quality of life,body weight,immunological indexes,the toxic and sub-effect,the blood rheology behavior,and compared with simple chemotherapy.Results The ratio of recurrence and transference of observed group within 1 or 2 years was respectively 25.74% and 31.43%,which was obviously lower than that of control group,which was 32.00%,52.00%(P

Objective To study the expression of PTEN et al and their significance in T lymphoblastic lymphoma/leukaemia (T-LBL/ALL). Methods Seventy-six cases of T-LBL/ALL were studied by using immunohistochemistry (EnVision method) for CD3, CD7, CD10, CD20, CD23, CD43, CD45RO, CD99, TdT, MPO and PTEN. Follow-up was included. Results In the Seventy-six cases of T-LBL/ALL, the percentages of tumor cells expressing TdT, CD99, CD3, CD7, CD45RO and CD43 were 93.42%, 94.74%, 67.12%, 92.11%, 36.85%,and 51.33%, respectivly while MPO, CD20 and CD23 were all negative. The index of Ki-67 expression higher than 80 % was found in 27 and ≤80% in 49 cases. The expression of PTEN (64.47 %) in T-LBL/ALL was lower than that of in reactivated lymphoid tissue (100%, P<0.05). PTEN was reversely correlated with stage,Ki-67 expression and LDH level (P<0.05). CD3-positive cases were more than CD3-negative cases in abnormal LDH level (P<0.05). Follow-up data was obtained in 50 cases, ranging from 1 to 108 months. The overall survival rate was 35.8 %, with median survival time 330 days. No prognostic significance was found in the expression of PTEN, CD3, CD7, CD10, CD43, Ki-67 and CD45RO (P >0.05). Conclusion The antibodies of CD3,CD7, CD10, CD20, CD43, CD45RO, CD99, TdT, MPO and Ki-67 were very helpful for the diagnosis of T-LBL/ALL.Down-regulation of PTEN may play an important role on the development of T-LBL/ALL.